The Monoclonal Antibody core in the Interdisciplinary Center for Biotechnology Research (ICBR) is hiring a Core Research Facility Technician III. ICBR is a fee for service science center focused on enabling scientific discovery and innovation with good customer service and access to world-class biotechnology expertise and instrumentation. 

Job Duties:

– Rodent handling and non-surgical procedures for the purpose of generating unique antibody-producing hybridoma cell lines. Occasional after hours, weekend, and holiday post procedure monitoring required.

– Develop and perform biochemical assays (e.g., ELISA and western blot). Analyze experimental data. Keep legible, accurate and complete records.

– Serve as a contact for REES equipment alarms after hours, weekends, and holidays, including troubleshooting equipment malfunctions during nonbusiness hours. Perform the following routine lab maintenance: maintain cryogenic freezers, move compressed gas tanks liquid nitrogen (300 lbs.) and carbon dioxide (145 lbs.), autoclave, wash labware, prepare lab solutions, decontaminate incubators.

– Perform affinity chromatography for monoclonal/polyclonal antibody purification. Develop and optimize purification methods. Analyze quality, quantity, and activity of purified antibody. Aliquot sterile culture media and chemical reagents. The understanding/practice of aseptic technique and record keeping is essential. Culture and maintain hybridoma and recombinant antibody cell lines.

– Plan, organize and carry out assigned lab and administrative tasks. Present data and generate electronic notes during lab meetings. Manage projects and communicate frequently with each client. Enter service requests using the CrossLab (iLab) system. Perform intake functions such as receiving and logging samples or requests for services. Ensure that timely progress is made on all assigned projects.

– Maintain information on each new hybridoma cell line and storage locations using an electronic inventory system. Periodically purge un-cloned hybridoma cell lines from cryo-storage.

– Other duties assigned such as maintain chemical inventory and manage chemical waste pickup. Complete monthly hazardous waste checklist. Acting instructor of Mammalian Cell Culture Workshop

The NextGen DNA Sequencing core in the Interdisciplinary Center for Biotechnology Research (ICBR) is hiring a Core Research Facility Technician III. ICBR is a fee for service science center focused on enabling scientific discovery and innovation with good customer service and access to world-class biotechnology expertise and instrumentation. 

Job Duties:

-Technical Functions
Plan and execute Illumina MiSeq, iSeq, NovaSeq and ONT sequencing runs.
Maintain and troubleshoot Illumina MiSeq, iSeq, NovaSeqand ONTinstruments.
Evaluate MiSeq, iSeq, NovaSeqand ONT instruments data and prepare written reports for research projects.
Construct and perform quality assessment of samples, and sequence libraries for the ONT, PacBio and Illumina platforms.
Test, troubleshoot and implement new protocols for NGS library construction protocol, using novel procedures as they become available in the literature or from vendors.
Perform all basic QC assays that are part of sample preparation processes for NGS, including, but not limited to: Fluorometric assays (on the QUBIT and FLUOstar Omega BMG), size selection protocol (on the SAGE-ELF and gels, magnetic bead purification (AMPure), and DNA fragmentation protocols (Covaris S220 and Covaris E220).
Document all work by using established lab tracking sheets (LTS) and digital documentation tools (FileShare).
Communicate with supervisor and other core personnel about experimental results in a timely and professional manner. Work with supervisor to define deliverables and timelines for project completion.
Contribute to the preparation, updating and organization of Standard Operating Procedures (SOPs).

-General Lab Operation and Maintenance Functions
Evaluate new laboratory methods for the implementation of new services in the NGS core.
Contribute to the development and maintenance of lab tracking system (LIMS) in the NGS workflows.
Attend all relevant coordination and organizational meetings as requested by supervisor.
Contribute to the maintenance of a complete inventory of reagents, consumables and equipment used for NGS sequencing.
Assist lab manager in keeping track of inventories and placing orders of reagents, consumables for ONT, PacBio and Illumina sequencing as needed.
Assist the Environmental Health and Safety (EHS) manager for the NextGen lab as needed, for the purpose of complying with all EHS institutional requirements.

– Educational Functions
Keep abreast of new developments in the NGS field through the literature and scientific meetings.
Help in the training of other personnel in the NGS Core group.
Participate in cross-training activities in the NGS core and ICBR as necessary or as requested by lab director.

– Misc.
Help in laboratory organization, cleaning and general maintenance
Receive, store, and help document orders arriving into the lab. Help send off outgoing parcels when requested.
– Other duties as assigned