Nikon Ti-E “Live Cell” Widefield Microscope (Motorized)
Inverted widefield microscope with motorized XY stage for fluorescent or brightfield samples. Available Tokai-Hit stage top incubator with active CO2 and temperature regulation and passive humidity, for imaging live cells in a 35mm dish or a chambered coverglass. Motorized XY stage movement for multi-point time-lapse or tile-mapping (AKA mosaic or stitching.)
Objectives: 10x dry, 20x dry, 40x dry and 60x water.
Software: Nikon NIS-Elements v4.51 on a Windows 7 workstation. This software is also available (with the optional Deconvolution module) on our separate Windows 7 Analysis Workstation.
Multi-line LED lamp for fluorescent excitation
Fluorescent filter sets: DAPI, GFP/FITC, TRITC, Texas Red, and CY5
Phase Contrast optics for 10x and 20x
DIC (Nomarski) optics for 40x and 60x
Two cameras: Andor Zyla sCMOS 11 bit monochrome for fluorescence and a Nikon DS-Fi2 color camera for stained brightfield samples
Location: CTAC Imaging Suite (MBI LG-164)