High content screening offers robust, multi-parametric and scalable analysis of large numbers of cells in heterogenous populations with the benefit of spatial and temporal resolution afforded by fluorescence microscopy. This seminar will provide an overview of practical considerations for fluorescence labeling and detection, as well as newly developed probes and assays for various aspects of cell structure, health, and function using both traditional fluorescence microscopy and high content imaging and analysis. Some emphasis will be given to visualization of cell stress and death mechanisms, proliferation, and internalization, including tools for immuno-oncology applications in both 2D and 3D models.

Coffee and doughnuts starting at 9:00 am, seminar beginning at 9:30 am.

Speaker: Michael S. Janes. M.S.

Mike received his M.S. in 1996 from Bowling Green State University in the United States, where his graduate work focused on mitochondrial biology and comparative biochemistry between parasitic helminths and their mammalian hosts. Mike continued his training as a cell biologist and microscopist at National Jewish Medical Center in Denver, Colorado where he developed fluorescence-based assays for the study of inflammation and apoptosis in respiratory diseases until 2000. He has been a research scientist at Thermo Fisher Scientific developing reagents and assays for fluorescence microscopy since 2000. Mike currently leads the Scientist to Scientist (S2S) Program, intended to connect R&D scientists at Thermo Fisher Scientific with researchers across the globe to drive technical exchange and develop collaborations.

Date: June 13