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BioRad Real Time Digital PCR
September 12, 2019 @ 12:00 pm - 1:00 pm
Discover the Power of Droplet Digital PCR: Come hear why researchers are turning to droplet digital real-time PCR technology vs traditional real-time PCR
Please join us for a special lunch and learn with ICBR Gene Expression & Genotyping and BioRad in CGRC 451
Digital PCR enables the absolute quantitation of nucleic acids in a sample. The lack of scalable and practical technologies for digital PCR implementation has hampered the widespread adoption of this inherently powerful technique. This talk will provide a technical overview of a commercially available droplet digital PCR (ddPCR™) system that enables processing of 2 million PCR reactions using conventional TaqMan assays with a 96-well plate workflow. Data from key applications will be presented to demonstrate that the massive partitioning afforded by our ddPCR system provides orders of magnitude more precision and sensitivity than real-time PCR. First, we show the accurate measurement of germline copy number variation. Second, for rare alleles, we show sensitive detection of mutant DNA in a 100,000-fold excess of wildtype background. Third, we demonstrate the power of absolute quantitation for applications including sequencing library quantitation and measuring miRNAs in plasma. We anticipate this ddPCR system will allow researchers to explore complex genetic landscapes, discover and validate new disease associations, and define a new era of molecular diagnostics.
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