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ThermoFisher HPLC and Mass Spectrometry
February 13 @ 12:00 pm - 1:30 pm
Join our Proteomics & Mass Spectrometry core and ThermoFisher for this seminar featuring two exciting talks:
“Understanding Column Chemistries in Metabolomics Workflows”
Metabolomics is the large-scale study of small molecules, commonly known as metabolites, within cells, biofluids, tissues or organisms. Collectively, these small molecules and their interactions within a biological system are known as the metabolome. Metabolites play an important role in a diverse range of applications. There are numerous fields of work that rely on successful small-molecule identification and characterization. However, even with advanced LC/MS technologies, the biomarker discovery using untargeted metabolomics remains challenging. The class of compounds being analyzed will determine how to proceed for chromatographic separation and the column chemistry that is needed for analysis. The importance of determining the proper column chemistry plays a vital role in the proper identification of metabolites.
Speaker: Dr. Pryor Katie
“Metabolomic Analysis Using Mass Spectrometry”
Metabolomic workflows mixing high resolution OrbitrapTMplatforms and low resolution triple quadrupole platforms with data analysis from targeted, untargeted and mixed mode methods will be demonstrated and explained using plant metabolomic data that has been created here at the University of Florida through collaborations with Thermo Fisher Scientific. Modern Vanquish™ Horizon UHPLC Systems employing reverse phase, HILIC, and hydrophobic long chain stationary phases will show how multiple chromatographic approaches can increase the depth of identified endogenous metabolites with high precision quantification. Library building with TraceFinderTM and XcaliburTM will complement untargeted analysis using Compound DiscovererTM, Lipid SearchTM, and mzCloudTM and will be used to drive mixed mode data dependent methods utilizing ion trapping type instruments.