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Zeiss on Your Campus (Seminar and Demonstration)

August 13, 2019 @ 8:00 am - August 14, 2019 @ 5:00 pm

Zeiss on your Campus

Please join Zeiss and ICBR Cytometry for this exciting series of talks and demonstrations in CGRC 101 and 184

August 13th:

10am: Optics in Microscopy

11am: Optimizing Imaging Parameters and Live Cell Imaging

12:30-5:00pm: Hands On Training

 

On August 14th

10-12:30pm: Optical sectioning methods and super-resolution microscopy.

12:30-5:00pm: Hands On Training

 

Hands On Sessions

For our hands on sessions on August 13/14th from 12:30-5:00pm- They will be in CGRC 184.

Zeiss will bring three different microscopes including the LSM980 which are all running on the same software (ZEN blue) so that the students can get comfortable very quick.

We would like to have the participants enrolled in all three rotations (one full day for a set of rotations) so that everyone sees all three stations.

Here are some of the aspects that will be discussed during the hands on sessions on August 13th and 14th in the afternoon:

On Widefield systems:

  • Discuss PSF to demonstrate effect of NA and resolution
  • Camera binning and ROI imaging
  • Incubation components. Immersol W. Oil vs Water lens comparison
  • Discuss influence of cameras and objectives on exposure times and sample preservation.
  • Live cell imaging including DIC vs Phase vs BF – Discuss benefits of LED and multiband pass imaging
  • Discuss imaging through plastic vs glass. #1.5 coverslip, sample prep, phenol red free imaging etc

On the LSM980 confocal Microscope:  

  • Selecting excitation and emission wavelength, Nyquist sampling –low laser power – sensitivity –SNR
  • Spatial and temporal resolution. Emphasize #1.5 and its significance.
  • Gentle live cell imaging with high resolution (3D) – Briefly discuss bleaching in TL imaging

 

The rotations are planned for Tuesday and Wednesday after the talks.

Thursday and possibly in Friday morning we will have more hands on time for interested researchers to bring their own samples and do imaging with assistance on the instruments.

Seminars

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Details

Start:
August 13, 2019 @ 8:00 am
End:
August 14, 2019 @ 5:00 pm

Organizer

ICBR Cytometry
Phone:
(352) 273-8186
Email:
ICBR-Cytometry@ad.ufl.edu
Website:
https://biotech.ufl.edu/cytometry/

Venue

CGRC Room 101
2033 Mowry Road
Gainesville, FL 32610 United States
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