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Illumina MiSeq i100

Illumina MiSeq i100

Access focused applications such as targeted resequencing, metagenomics, small whole-genome sequencing, targeted gene expression profiling, and more. MiSeq i100 reagents enable up to 30 Gb of output with 100 million sequencing reads and 2 × 300 bp read lengths.

The MiSeq i100 Series are powered by XLEAP-SBS chemistry, the fastest, most accurate, Illumina sequencing by synthesis (SBS) chemistry to date. Improved robustness and stability of XLEAP-SBS reagents allow for shipping and storage at room temperature, expediting run setup without waiting for reagents to thaw. MiSeq i100 Series generates highly accurate data with a minimum specification of > 90% of bases above Q30. Enhanced usability features, including load-and-go consumables, onboard denaturation, onboard cluster generation, and no post-run washing, streamline the sequencing workflow.

Flow CellCyclesRead LengthReads Passing FilterOutputRun Time
5M3002 x 150 bp5 Million1.5 Gb7 hr
6002 × 300 bp3 Gb15 hr
25M1002 x 50 bp25 Million2.5 Gb4 hr
3002 x 150 bp7.5 Gb7 hr
6002 × 300 bp15 Gb15 hr
50M1002 x 50 bp50 Million5 Gb4.5 hr
3002 x 150 bp15 Gb7.5 hr
6002 × 300 bp30 Gb15.5 hr
100M1002 x 50 bp100 Million105 hr
3002 x 150 bp30 Gb8 hr