Spectral Instruments Imaging (SII) Lago Bioluminescence Imager

UF | ICBR Cytometry

Spectral Instruments Imaging (SII) Lago Luminescence Imager

Please note: This new Lago imager is different from the old IVIS imager, and utilizes different software. Users who were previously trained and operated the IVIS system will need to undergo a new user training session before accessing this instrument.

Luminescence Imager for measuring and quantifying fluorescent or chemiluminescent (Luciferin/Luciferase) signal expression in in-vivo, ex-vivo, or in-vitro specimens.

Software: SII Aura on a Windows 10 workstation.

LED Excitation Wavelengths:

360, 405, 430, 465, 500, 535, 570, 605, 640, 675, 710, 745, 770, and 805nm

Emission filters:

(Listed wavelength is the filter centerline.)

490, 510, 530, 550, 570, 590, 610, 630, 650, 670, 690, 710, 730, 750, 770, 790, 810, 830, 850, and 870nm

 

When performing in-vivo experiments using fluorescence, it is highly advisable to use a fluorescent label that emits above 600nm.

The SII Lago system includes an isoflurane vaporizer unit for in-vivo experiments. Users must provide their own isoflurane and charcoal scrubbers. Medical grade oxygen for running the vaporizer is provided with the system. Users must provide any other necessary reagents/supplies for prepping their samples.

Free copies of SII’s Aura analysis software may be obtained from SII’s website at:

https://spectralinvivo.com/software/

 

All In-vivo experiments require an approved IACUC protocol.

Location: CTAC Imaging Suite (MBI LG-164)

Software – Spectral Instruments Imaging

Aura Software enables you to capture and analyze your in vivo imaging data quickly, easily and accurately.