Monoclonal Antibody
Acknowledge Core
Proper Citation:
University of Florida ICBR Monoclonal Antibody Core Facility, RRID:SCR_019147
Contact
ICBR-MonoclonalAntibody@ad.ufl.edu
(352) 273-8040
(352) 273-8057
schen@ufl.edu
169A CGRC
My research is focused on the signaling and metabolic mechanisms underlying plant interaction with the environment. My lab research has been particularly focused on three topics: glucosinolate metabolism, guard cell signal transduction, and plant pathogen interaction.
0000-0002-6690-7612
Project 1. Glucosinolate metabolism. Glucosinolates are a group of naturally occurring thioglucosides, present in Brassica plants (e.g., canola and cabbage). Glucosinolate degradation products display diverse biological activities, including defense against insects and herbivores, N/S nutrition and growth regulation. From a human perspective, glucosinolate metabolites account for the distinctive flavors of cabbage and condiments. Some of the metabolites such as isothiocyanates exhibit anticarcinogenic properties. The core glucosinolate pathway has been well studied in Arabidopsis. However, we know little about how the components in different pathways interact to produce plant phenotypes and traits. Nor do we know how different layers of molecular control work together. The lack of such fundamental knowledge is a major reason why plant genetic engineering has been largely unsuccessful. It poses a chronic problem for rational engineering of crops for better quality and defense. Research in this project is focused on characterizing the regulatory and metabolic networks involving glucosinolate metabolism using multidisciplinary approaches. We aim to identify protein and metabolite changes in response to perturbation of glucosinolate metabolism and to integrate the data into glucosinolate networks. The process of networking will generate new testable hypotheses concerning glucosinolate metabolic pathways and related pathways. The ultimate objective is to use the immense biosynthetic potential of plants as an efficient, environmentally friendly and renewable source of fine chemicals and pharmaceuticals.
Project 2. Guard cell signaling networks. Guard cells are highly specialized plant epidermal cells that enclose tiny pores called stomata. Stomatal movements control both uptake of carbon dioxide and loss of water, and thus play important roles in plant growth and acclimation to environmental stresses. The plant hormone abscisic acid (ABA) is a key indicator of drought stress. ABA induces stomatal closure via an intricate intracellular signaling network in guard cells, thereby promoting plant water conservation. It is our central hypothesis that protein redox modification and dynamic changes in key metabolites are critical regulatory mechanisms in ABA signaling. We are testing the hypothesis by pursuing: identification of guard cell proteins whose redox status is altered in response to ABA and determination of their specific redox-sensitive amino acid residues, quantification of ABA-induced changes in metabolites implicated in guard cell signaling, and integration of the new information into a dynamic model of ABA-induced stomatal closure. Accomplishing these objectives is significant because it will reveal novel components of ABA signaling networks and provide knowledge of regulatory mechanisms underlying stomatal movements that will help to develop crops with enhanced stress tolerance and productivity.
Project 3. Plant pathogen interaction. The study of pathogen response and defense in crop species is of essential importance as the applications are directly related to agricultural production. Pseudomonas syringae pv tomato (Pst DC3000) causes speck disease in tomato (Solanum Lycopersicum), a crop growing in large quantities in Florida and having both nutritional and economical value. The goal of this project is to take what is known about pathogen host interactions and observe in greater detail mechanisms that plants utilize in response to pathogen infection at the posttranscriptional levels, including protein expression, redox and phosphorylation/dephosphorylation switches. Understanding changes in protein expression as well as redox and phospho-switches will provide important insights into how plant response and resistance to pathogens are occurring. Further investigation into unique/novel proteins and regulations will advance our knowledge of plant defense against pathogens, and allow researchers to use biotechnology to prevent future bacterial speck disease outbreaks.
Interestingly, as we gain more and more knowledge, the above projects have become interconnected with each other. Glucosinolate metabolism plays a role in pathogen defense and affects stomatal movement, which serves as the first line of defense against pathogen invasion. In addition to hypothesis generation projects, another major part of my research program has been hypothesis driven, i.e., characterizing molecular, biochemical and physiological functions of specific genes and proteins identified by proteomics and metabolomics approaches. One of the projects has been focused on understanding the key steps in the methionine chain-elongation pathway, which directly connects methionine (primary) metabolism to glucosinolate (spealized) metabolism. Our integration of hypothesis generation and hypothesis driven research will ultimately lead to a holistic view of cellular networks and processes in plants and will create important stepping stones towards potential biotechnological applications in enhanced yield, bioenergy and defense.
Name | Telephone | Room | Department | |
---|---|---|---|---|
UF | ICBR Administration | Administration | |||
Name/Title/Department | Phone | Location | Administration | |
Dr. Anna-Lisa Paul | (352) 273-8029 | alp@ufl.edu | 160 CGRC | Administration |
Dr. Steven Madore | (352) 273-8038 | smadore@ufl.edu | 156 CGRC | Administration |
Heather Voet | (352) 273-9461 | hvoet@ufl.edu | 161 CGRC | Administration |
Administration | ||||
Business Services | ||||
Name/Title/Department | Phone | Location | Business Services | |
Heather Voet Associate Director of Administration Business Services | (352) 273-9461 | hvoet@ufl.edu | 161 CGRC | Business Services |
Patrick Carrao Finance Manager Business Services | (352) 273-8027 | pbcarrao@ufl.edu | 163 CGRC | Business Services |
Erica Boyd Human Resources Generalist III Business Services | (352) 273-8271 | ericalboyd@ufl.edu | 158 CGRC | Business Services |
Tina Hasko Business Administrative Specialist III Business Services | (352) 273-8048 | thasko@ufl.edu | 154 CGRC | Business Services |
Mackenzie Madore Administrative Specialist II Business Services | (352) 273-8065 | mmadore@ufl.edu | 157 CGRC | Business Services |
Tensi Krpan Administrative Specialist II Business Services | (352) 273-8026 | tenser9@ufl.edu | 181 CGRC | Business Services |
Kimberly Backer-Kelley | (352) 273-5341 | klbk@ufl.edu | 185 CGRC | Business Services |
Business Services | ||||
Cyberinfrastructure | ||||
Name/Title/Department | Phone | Location | Cyberinfrastructure | |
Clinton Collins Cyberinfrastructure Director Cyberinfrastructure | (352) 273-9274 | clintc@ufl.edu | 155 CGRC | Cyberinfrastructure |
Ben Thebaut IT Analyst II Cyberinfrastructure | (352) 273-6814 | ben.thebaut@ufl.edu | 188 CGRC | Cyberinfrastructure |
Cameron Smith Systems Administrator/Programmer II Cyberinfrastructure | (352) 273-9651 | pc.smith@ufl.edu | 188 CGRC | Cyberinfrastructure |
Manuel Luciano Systems Administrator/Programmer II Cyberinfrastructure | (352) 273-8054 | manuel.luciano@ufl.edu | 188 CGRC | Cyberinfrastructure |
Daniel Tarlini IT Analyst I Cyberinfrastructure | (352) 273-8170 | tarlinid@ufl.edu | 188 CGRC | Cyberinfrastructure |
Cyberinfrastructure | ||||
UF | ICBR Communications | Communications | |||
Name/Title/Department | Phone | Location | Communications | |
Chris Dillon Communications Section Director Communications | (352) 273-8168 | csdillon@ufl.edu | 162 CGRC | Communications |
Communications | ||||
Bioinformatics | ||||
Name/Title/Department | Phone | Location | Bioinformatics | |
Dr. Alberto Riva Scientific Director Bioinformatics | (352) 294-5475 | ariva@ufl.edu | 191 CGRC | Bioinformatics |
Dr. Fahong Yu Scientist Bioinformatics | (352) 273-8064 | fyu@ufl.edu | 191 CGRC | Bioinformatics |
Dr. Tongjun Gu Assistant Scientist Bioinformatics | (352) 273-8058 | tgu@ufl.edu | 191 CGRC | Bioinformatics |
Bioinformatics | ||||
Cytometry | ||||
Name/Title/Department | Phone | Location | Cytometry | |
Dr. Mariza Miranda Scientific Director Cytometry | (352) 273-8185 | marizamiranda@ufl.edu | 292 CGRC | Cytometry |
Doug Smith Senior Laboratory Technician Cytometry | (352) 392-1745 | dugsmith@ufl.edu | LG-164 | Cytometry |
Dr. Daniil Shabashvili Core Research Specialist II Cytometry | (352) 273-8186 | CGRC 292 | Cytometry | |
Alexander Linares Core Research Facility Technician III Cytometry | (352) 273-6032 | a.linares@ufl.edu | CGRC 292 | Cytometry |
Cytometry | ||||
Electron Microscopy | ||||
Name/Title/Department | Phone | Location | Electron Microscopy | |
Paul R. Chipman Scientific Director Electron Microscopy | (352) 294-1790 | pchipman@ufl.edu | 1012 MCS | Electron Microscopy |
Karen Kelley Core Research Facility Specialist III Electron Microscopy | (352) 273-8547 | vau@ufl.edu | 1012 MCS / 185 CGRC | Electron Microscopy |
Rudy Alvarado Core Research Facility Specialist I Electron Microscopy | (352) 846-0251 | ralvaradojr@ufl.edu | 1012 MCS | Electron Microscopy |
Mary Gragg OPS Lab Technician Electron Microscopy | (352) 846-0251 | mary.gragg@ufl.edu | 1012 MCS | Electron Microscopy |
Electron Microscopy | ||||
Gene Expression & Genotyping | ||||
Name/Title/Department | Phone | Location | Gene Expression & Genotyping | |
Dr. Yanping Zhang Scientific Director Gene Expression & Genotyping | (352) 273-8043 | yanp@ufl.edu | 178F CGRC | Gene Expression & Genotyping |
Brandon Pate Core Research Facility Specialist II Gene Expression & Genotyping | (352) 273-8036 | Bpate1@ufl.edu | 178 CGRC | Gene Expression & Genotyping |
Mark Renshaw Core Research Facility Specialist II Gene Expression & Genotyping | (352) 273-8036 | marenshaw@ufl.edu | 178 CGRC | Gene Expression & Genotyping |
Yasemin Can Core Research Facility Technician III Gene Expression & Genotyping | (352) 273-8036 | ycan@ufl.edu | 178 CGRC | Gene Expression & Genotyping |
Sewande Mudiyanselage Student Assistant Gene Expression & Genotyping | (352) 273-8036 | s.mudiyanselage@ufl.edu | 178 CGRC | Gene Expression & Genotyping |
Paul Fernandez Student Assistant Gene Expression & Genotyping | (352) 273-8036 | fernandezp@ufl.edu | 178 CGRC | Gene Expression & Genotyping |
Ashlyn Lake Student Assistant Gene Expression & Genotyping | (352) 273-8036 | ashlynlake@ufl.edu | 178 CGRC | Gene Expression & Genotyping |
Gene Expression & Genotyping | ||||
Monoclonal Antibody | ||||
Name/Title/Department | Phone | Location | Monoclonal Antibody | |
Dr. Angel Sampson Scientific Director Monoclonal Antibody | (352) 273-8039 | angel.sampson@ufl.edu | 175 CGRC | Monoclonal Antibody |
Shadi Bootorabi Senior Biological Scientist Monoclonal Antibody | (352) 273-8041 | shb@ufl.edu | 171A CGRC | Monoclonal Antibody |
Katrina Witas Core Research Facility Technician III Monoclonal Antibody | (352) 273-8040 | kaekelly@ufl.edu | 171 CGRC | Monoclonal Antibody |
Lourdes Guallpa Student Assistant Monoclonal Antibody | (352) 273-8040 | lguallpa@ufl.edu | 171 CGRC | Monoclonal Antibody |
Monoclonal Antibody | ||||
NextGen DNA Sequencing | ||||
Name/Title/Department | Phone | Location | NextGen DNA Sequencing | |
Dr. David Moraga Scientific Director NextGen DNA Sequencing | (352) 273-8053 | moraga@ufl.edu | 178J CGRC | NextGen DNA Sequencing |
Dr. Diansy Zincke Core Research Facility Specialist III NextGen DNA Sequencing | (352) 273-8050 | dzincke@ufl.edu | 178 CGRC | NextGen DNA Sequencing |
Dr. Liming Zhao Core Research Facility Specialist II NextGen DNA Sequencing | (352) 273-8050 | lmzhao@ufl.edu | 178 CGRC | NextGen DNA Sequencing |
Xiao Hui Zhou Core Research Facility Technician III NextGen DNA Sequencing | (352) 273-8050 | zxh@ufl.edu | 178 CGRC | NextGen DNA Sequencing |
Benjamin Hoffman Core Research Facility Technician III NextGen DNA Sequencing | (352) 273-8050 | semplar@ufl.edu | 178 CGRC | NextGen DNA Sequencing |
Daina Elsayed OPS Lab Technican NextGen DNA Sequencing | (352) 273-8050 | relsayed@ufl.edu | 178 CGRC | NextGen DNA Sequencing |
NextGen DNA Sequencing | ||||
Proteomics & Mass Spectrometry | ||||
Name/Title/Department | Phone | Location | Proteomics & Mass Spectrometry | |
Dr. Jin Koh Scientific Director Proteomics & Mass Spectrometry | (352) 273-8059 | jinkoh@ufl.edu | 168A CGRC | Proteomics & Mass Spectrometry |
Ran Zheng Core Research Facility Specialist II Proteomics & Mass Spectrometry | (352) 273-8061 | ranzheng@ufl.edu | 169 CGRC | Proteomics & Mass Spectrometry |
Ning Zhu Core Research Facility Specialist II Proteomics & Mass Spectrometry | (352) 273-8067 | nzhu@ufl.edu | 169 CGRC | Proteomics & Mass Spectrometry |
Fanchao Zhu Core Research Facility Specialist I Proteomics & Mass Spectrometry | (352) 273-8060 | fzhu9482@ufl.edu | 169 CGRC | Proteomics & Mass Spectrometry |
Proper Citation:
University of Florida ICBR Monoclonal Antibody Core Facility, RRID:SCR_019147
ICBR-MonoclonalAntibody@ad.ufl.edu
(352) 273-8040