Nikon Ti-E Widefield Microscope

Inverted widefield microscope with motorized XY stage for fluorescent or brightfield samples. Available Tokai-Hit stage top incubator with active CO2 and temperature regulation and passive humidity, for imaging live cells in a 35mm dish or a chambered coverglass. Motorized XY stage movement for multi-point time-lapse or tile-mapping (AKA mosaic or stitching.)

Objectives: 10x dry, 20x dry, 40x dry and 60x water.

Software: Nikon NIS-Elements v4.51 on a Windows 7 workstation. This software is also available (with the optional Deconvolution module) on our separate Windows 7 Analysis Workstation.

Features:
Multi-line LED lamp for fluorescent excitation
Fluorescent filter sets: DAPI, GFP/FITC, TRITC, Texas Red, and CY5
Phase Contrast optics for 10x and 20x
DIC (Nomarski) optics for 40x and 60x

Two cameras: Hamamatsu Orca Flash 4.0 sCMOS monochrome for fluorescence and a Nikon DS-Fi2 color camera for stained brightfield samples

Hamamatsu Orca Flash 4.0, Nikon DS-Fi2 Color 5.24 Megapixels camera.

NIS-Elements Image Capture and Analysis Software.

Equipped with a stage-top Tokai Hit Incubation Chamber and digital gas mixer, with sensor feedback system for the Elements software. Holds one multi-well culture plate or two 35mm dishes.

Nikon Perfect Focus combats axial focus flux in real time.
Objectives

MRH10101 CFI Plan Fluor DLL 10X Objective Lens NA 0.3 WD 16MM, Eco-Glass
MRD30205 CFI Plan Apochromat Lambda DM 20x Objective Lens NA 0.75 WD 1.00MM
MRD00405 CFI Plan Apochromat Lambda 40x Dry Objective Lens NA 0.95 WD 0.21MM
MRD07602 CFI Plan Apo VC 60X Water Immersion (WI) Objective Lens NA 1.2 WD0.27MM, Eco-Glass

Fluorescent Filter Sets

Spectra X; Polychroic and quad bandpass emitter for use with the following single bandpass filters: ET395/25x, ET470/24x, ET550/15x,ET640/30x
Spectra X; Polychroic and triple bandpass emitter; CFP/YFP/M-Cherry
Custom DAPI Filter Set for use with SOLA, Exciter: ET395/25x, 25mmDichroic: T425LPXREmitter: ET460/50m, 25mm
FITC/RSGFP/BO FLTR ST MTD CB
TRITC (Rhodamine with Narrow-Band Excitation Filter) Filter Set,Ti Cube.Exciter: HQ545/30x, 25mmDichroic: Q570LPEmitter: HQ610/75m
41025 Piston GFP Bandpass Filter Set. Exciter: HQ470/40x,25mm Dichroic: Q495LP Emitter: HQ515/30m
TXRD FLTR ST MTD CUBE
C-FL CY5 HYQ

Location: CTAC Imaging Suite (MBI LG-164)