Proteomics & Mass Spectrometry

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PROTEOMICS & MASS SPECTROMETRY: Home | Staff & Contact | Services | Instruments | Core Resources

Email: ICBR-Proteomics@ad.ufl.edu Phone: (352) 273-8060

Overview

ICBR Proteomics & Mass Spectrometry Facility has provided unprecedented tools for fast, accurate, high throughput biomolecular separation and characterization, which are indispensable towards understanding the biological and medical systems. Studying at the protein level allows researchers to investigate how proteins, their dynamics, modifications, and interactions affect cellular processes and how cellular processes and environment affect proteins. The mission of our facility is to provide excellent service and training in proteomics and mass spectrometry. Here we present our capabilities in proteomics, highlighting differential proteomics, which include two-dimensional difference gel electrophoresis (2D-DIGE), isobaric tags for relative and absolute quantitation (iTRAQ), tandem mass tags (TMT), iodoTMT, Stable isotope labeling with amino acids in cell culture (SILAC), quantification of proteins by label-free LC-MS/MS, and the analysis of protein post-translational modifications (PTM).

We have a suite of state-of-the-art mass spectrometers and software available for detailed characterization of proteins and peptides, including post-translational modification analysis and accurate molecular weight determination. To ensure success and maximize productivity, the Proteomics & Mass Spectrometry core offers education, consultation, data processing and reporting, and support of grant application.

Core News

UPDATE: ICBR Continues to Provide Support to UF Community

With the progression of recent events, there are a lot of questions about how it affects ICBR users. Please see below, important changes that could impact your experience at ICBR. These changes will be in place for the foreseeable future. Please refer back to this page for timely updates. As always, check the UF dedicated site for campus-wide information and initiatives. LATEST NEWS ICBR is now open, Monday through Friday, to support research on the University of Florida campus. Please be...

Dr. Anna-Lisa Paul Appointed Director of UF ICBR

The University of Florida Interdisciplinary Center for Biotechnology Research recently announced the appointment of its new director, Anna-Lisa Paul, Ph.D. Dr. Paul assumed the position effective March 20, 2020. For the past year, Dr. Paul has served as ICBR’s interim director. During that time, Dr. Paul spearheaded the center’s acquisition of state-of-the-art equipment and furthered ICBR’s collaborative relationship with UF Research departments. “The University of Florida Health Cancer ...

BI Tongjun New Pub

Bioinformatics assistant scientist Tongjun Gu Ph.D. has developed a two-step logistic regression model for association studies where known factors can serve as controls. The model is helpful for studies seeking to identify novel regulators in the RNA editing process where the dominant enzyme was found in the first step and served as a control in the second step. Advantages of the model: It considered the sequencing depth in the analysis. It can be validated in the first step by the association ...

BI assistant scientist develops a tool for stratifying cancer patients

Bioinformatics assistant scientist Tongjun Gu Ph.D. has developed an advanced deep learning method to stratify cancer patients into clinically relevant subgroups and further identify the potential biomarkers between the subgroups. This will help with the development of personalized treatments and prognosis. The method can integrate multi-platform datasets like gene expression, miRNA expression, methylation, copy number alteration, and protein expression. It can also do transfer learning between ...

ICBR Proteomics & Mass Spectrometry (PM) aims to bring single-cell proteomics and metabolomics technologies to customers

The cell is the basic unit of life. For multicellular organisms like animals and plants, each cell is different. Due to limited sensitivity, conventional ‘omic analyses require the input of tissues, organs or a mixture of thousands of cells, where information at the single-cell level has been diluted and averaged. This problem has severely limited our understanding of the intricate signaling and metabolic pathways and networks in different cells. In recent years, single-cell biology has become...

Important Upcoming Billing Dates

University of Florida and ICBR core labs will be officially closed during the UF holiday: Wednesday, December 25, through Wednesday, January 1. No samples or other packages can be accepted during this holiday closure time. Please coordinate with individual core laboratories for delivery of samples or other packages either before or after the UF winter break. Due to the winter break, ICBR’s upcoming billing schedule will be altered as follows: 12/16/2019 – Last invoices generated in 2019...

A New Method to Examine Human Dopamine Transporter in Peripheral Blood

A team of UF neuroscientists led by Habibeh Khoshbouei, Ph.D., Pharm.D., Professor of Neuroscience and Psychiatry in the UF College of Medicine, has recently discovered a novel method using blood-borne immune cells to study the role of the neurotransmitter dopamine in the human brain. This finding was reported in a publication titled ”A novel approach to study markers of dopamine signaling in peripheral immune cells” appearing in the Journal of Immunological Methods. While immunohistochemi...

Dr. Tongjun Gu, from ICBR Bioinformatics, Improves Discovery Pipeline

Bioinformatics assistant scientist Tongjun Gu Ph.D. has improved the pipeline for discovering ncRNA editing sites from four human cancers. Published in JCO Clinical Cancer Informatics, Clinical Relevance of Noncoding Adenosine-to-Inosine RNA Editing in Multiple Human Cancers, Dr. Gu’s research also presents multiple advanced statistical methods for differential and survival analysis. More information: Bioinformatics Services Contact Bioinformatics ...

Instruments

Staff

Name	Title	Telephone	E-mail	Room
Sixue Chen, Ph.D.	Proteomics & Mass Spectrometry Faculty Director	(352) 273-8057	schen@ufl.edu	169A CGRC
Jin Koh, Ph.D.	Proteomics & Mass Spectrometry Scientific Director	(352) 273-8059	jinkoh@ufl.edu	168A CGRC
Ran Zheng, M.S.	Proteomics & Mass Spectrometry Core Research Specialist I	(352) 273-8061	ranzheng@ufl.edu	169 CGRC
Ning Zhu, B.S.	Proteomics & Mass Spectrometry Core Research Specialist I	(352) 273-8067	nzhu@ufl.edu	169 CGRC
Fanchao Zhu, B.S.	Proteomics & Mass Spectrometry Core Research Technician III	(352) 273-8060	fzhu9482@ufl.edu	169 CGRC