Proteomics & Mass Spectrometry

Home

PROTEOMICS & MASS SPECTROMETRY: Home | Staff & Contact | Services | Instruments | Core Resources

Email: ICBR-Proteomics@ad.ufl.edu Phone: (352) 273-8060

Overview

ICBR Proteomics & Mass Spectrometry Facility has provided unprecedented tools for fast, accurate, high throughput biomolecular separation and characterization, which are indispensable towards understanding the biological and medical systems. Studying at the protein level allows researchers to investigate how proteins, their dynamics, modifications, and interactions affect cellular processes and how cellular processes and environment affect proteins. The mission of our facility is to provide excellent service and training in proteomics and mass spectrometry. Here we present our capabilities in proteomics, highlighting differential proteomics, which include two-dimensional difference gel electrophoresis (2D-DIGE), isobaric tags for relative and absolute quantitation (iTRAQ), tandem mass tags (TMT), iodoTMT, Stable isotope labeling with amino acids in cell culture (SILAC), quantification of proteins by label-free LC-MS/MS, and the analysis of protein post-translational modifications (PTM).

We have a suite of state-of-the-art mass spectrometers and software available for detailed characterization of proteins and peptides, including post-translational modification analysis and accurate molecular weight determination. To ensure success and maximize productivity, the Proteomics & Mass Spectrometry core offers education, consultation, data processing and reporting, and support of grant application.

Core News

Novel CD33 Antibodies Unravel Localization, Biology, and Therapeutic Implications of CD33 Isoforms

UF | ICBR Drs. Angel Sampson and Andria Doty recently teamed up with Dr. Jatinder Lamba in the Department of Pharmacotherapy and Translational Research in the UF College of Pharmacy, and Dr. Soheil Meshinchi at the Fred Hutchinson Cancer Research Center, University of Washington, in an effort to further understand the therapeutic relevance of various forms of the CD33 protein as it relates to the antibody-drug therapy. CD33 is expressed on malignant cancer cells in nearly all patients with ac...

Featured Video: Black Voices in Research

As part of its mission to facilitate honest and vulnerable conversations about race, the University of Florida Clinical and Translational Science Institute Diversity and Cultural Competence Council (UF CTSI DC3) presents its inaugural Black Voices in Research Storytelling Event. This event and others like it will serve to underscore the inherent value of sharing personal stories and the role that these stories can play in creating and promoting an inclusive, diverse, equitable, and accessible ...

UF | ICBR Staff Answer Challenge to Support Local Students in Need

For the third consecutive year, UF | ICBR held their annual food drive to provide food and supplies to Gainesville grade schools (event concluded 12/4). Well, although our results were tremendous in 2018 and 2019, this year took the cake. Over the course of eight weeks, our staff of 46 individuals collected over 5,150 items for Gainesville students, or approximately 112 items per employee. UF | ICBR’s HR Manager, Erica Boyd, led the charge; generating buzz, building rapport with nearby sch...

Important Year-End Dates for UF | ICBR Users

As 2020 comes to a close, ICBR’s upcoming billing schedule will be altered as follows: 12/14/2020 – Last invoices generated in 2020 12/21/2020 at noon – Deadline to submit disputes for 2020 payment. If you have expiring funds, please work with your core laboratory contact to make sure your project is completed and billed before the 12/14/2020 invoices are posted. Core-Specific Dates and Deadlines Cytometry 12/22/2020 – CY labs, both CGRC and MBI, will shut down for the holiday break and...

Excessive Heat Stroke Induces Long‐term Physiological Changes That are Linked to Altered Epigenetic Profiles

Epigenetics is the study of heritable phenotypic change that involves chemical modifications to the DNA genome sequence or associated DNA-binding proteins. These specific alterations affect how cells turn genes off or on. Exertional heat stroke (EHS) can cause long-term negative effects on health. Although epigenetic “reprogramming” of the genome is involved in the response to heat acclimation, it is not known whether the long-term effects of EHS are mediated by epigenetic reprogramming. In a st...

Lots of Samples, Little Time? Try Protein Simple Wes

Unlike traditional western blots, the Protein Simple Wes at ICBR Monoclonal Antibody runs up to 25 samples in 3 hours flat and gives you quantitative, size-based data including total protein. With a long list of applications, including cancer, SARS-CoV-2, cell & gene therapy, targeted degradation and more, there is no shortage of opportunities. The same Protein Simple Wes appeared in a recent publication featured in Nature Communications from Dr. Shinichi Someya’s research group in the Depar...

Are BPA Alternatives Safer? New Research Takes a Look

New research conducted at the University of Florida shows that a bisphenol A (BPA) alternative for making plastics, bisphenol S (BPS), could come with its own set of health risks. After past studies revealed the widely used BPA was contaminating food and water supplies, consumers quickly searched for alternatives. Market demand for BPA-free products led to the emergence of BPS as a commonly used bisphenol analog in plastic products. The UF | ICBR Proteomics and Mass Spectrometry (PM) core contri...

UPDATE: UF | ICBR Continues to Provide Support to UF Community

With the progression of recent events, there are a lot of questions about how it affects ICBR users. Please see below, important changes that could impact your experience at ICBR. These changes will be in place for the foreseeable future. Please refer back to this page for timely updates. As always, check the UF dedicated site for campus-wide information and initiatives. LATEST NEWS ICBR is now open, Monday through Friday, to support research on the University of Florida campus. Please be...

Instruments

Staff

Name	Title	Telephone	E-mail	Room
Sixue Chen	Proteomics & Mass Spectrometry Faculty Director	(352) 273-8057	schen@ufl.edu	169A CGRC
Jin Koh	Proteomics & Mass Spectrometry Scientific Director	(352) 273-8059	jinkoh@ufl.edu	168A CGRC
Ran Zheng	Proteomics & Mass Spectrometry Core Research Specialist I	(352) 273-8061	ranzheng@ufl.edu	169 CGRC
Ning Zhu	Proteomics & Mass Spectrometry Core Research Specialist I	(352) 273-8067	nzhu@ufl.edu	169 CGRC
Fanchao Zhu	Proteomics & Mass Spectrometry Core Research Technician III	(352) 273-8060	fzhu9482@ufl.edu	169 CGRC