Proteomics & Mass Spectrometry

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PROTEOMICS & MASS SPECTROMETRY: Home | Staff & Contact | Services | Instruments | Core Resources

Email: ICBR-Proteomics@ad.ufl.edu Phone: (352) 273-8060

Overview

ICBR Proteomics & Mass Spectrometry Facility has provided unprecedented tools for fast, accurate, high throughput biomolecular separation and characterization, which are indispensable towards understanding the biological and medical systems. Studying at the protein level allows researchers to investigate how proteins, their dynamics, modifications, and interactions affect cellular processes and how cellular processes and environment affect proteins. The mission of our facility is to provide excellent service and training in proteomics and mass spectrometry. Here we present our capabilities in proteomics, highlighting differential proteomics, which include two-dimensional difference gel electrophoresis (2D-DIGE), isobaric tags for relative and absolute quantitation (iTRAQ), tandem mass tags (TMT), iodoTMT, Stable isotope labeling with amino acids in cell culture (SILAC), quantification of proteins by label-free LC-MS/MS, and the analysis of protein post-translational modifications (PTM).

We have a suite of state-of-the-art mass spectrometers and software available for detailed characterization of proteins and peptides, including post-translational modification analysis and accurate molecular weight determination. To ensure success and maximize productivity, the Proteomics & Mass Spectrometry core offers education, consultation, data processing and reporting, and support of grant application.

Core News

UF Researcher Dr. Marco Salemi Utilizes ICBR Genomics Expertise to Understand COVID19 Epidemiology

By Steven Madore, Ph.D. In recent years, public health emergencies caused by epidemics have led to the use of genome sequencing to identify and characterize viral pathogens. Rapid acquisition of high quality viral genomic sequences is critical for understanding viral pathogen origin, transmission and epidemiological spread. Ultimately deciphering the molecular characteristics of viruses accelerates the development of diagnostic assays and vaccine development and drug design and is important in u...

UF researchers use AI datasets to track feral pigs, minimize disease risk

Courtesy of UF/IFAS Written by Brad Buck Feral pigs cost the agriculture industry at least $1.5 billion in damage, disease and control costs around the United States annually, running rampant on large swaths of grazing lands. The swine root in soil and eat most everything in sight. Farmers and ranchers will benefit from research by University of Florida scientists who are using artificial intelligence to gather data on feral hog reproduction and movement. As they roam grazing land, feral swine ...

UF | ICBR Scientists Contribute to Clinical Trial Testing Novel Treatment for Canine Atopic Dermatitis

Atopic dermatitis, or AD, is a common chronic inflammatory skin disease affecting dogs, causing defects of the skin barrier and modifications of the normal cutaneous microflora. While multiple treatment options are available, there is a growing interest in the use of alternative natural ingredient products. In human AD reports show the potential benefits of topical applications of heat-killed beneficial bacteria, with some studies demonstrating the efficacy of rapidly killed beneficial la...

UF researchers to use AI to predict how hurricanes spread invasive plants

Courtesy of UF/IFAS Written by Brad Buck Scientists project hurricane intensity and frequency will increase with climate change. That leads researchers to want to better predict how storms will disperse and establish nonnative plant invaders. Knowing where invasive plants spread will always be useful. But it may become especially handy soon as the National Hurricane Center is considering moving the official start of the Atlantic hurricane season two weeks earlier, starting it May 15, instead of ...

World-Renowned UF Health Biochemistry Researcher Passes Away

Courtesy of College of Medicine News March 3, 2021 — University of Florida Health biochemistry professor Mavis Agbandje-McKenna, Ph.D., whose world-renowned work on the detailed structure of viruses led to advances in gene therapy treatments for different diseases, died Wednesday at her home near Gainesville of the neurodegenerative disease amyotrophic lateral sclerosis. She was 57. Agbandje-McKenna’s work with the small, infectious particles had a worldwide impact, allowing other scientists to ...

UF Health pilot program to deliver COVID-19 monoclonal antibody therapy to rural communities

Courtesy of UF Health Written by Diana Tonnessen A University of Florida Health pilot program will bring a new COVID-19 monoclonal antibody therapy to rural communities in North Florida during the coming weeks. As part of the White House’s Operation Warp Speed initiative, the U.S. Department of Health and Human Services selected UF Health and the UF Clinical and Translational Science Institute to coordinate the distribution and administration of 1,000 doses of bamlanivimab, a monoclonal antibody...

UF offers powerful new NVIDIA supercomputer to students and researchers across State University System

Courtesy of UF NEWS Written by Brittany Wise University of Florida’s new supercomputer — the fastest artificial intelligence supercomputer in higher education — will soon be available for students and faculty across the State University System, UF Provost Joe Glover announced today. The computer will give faculty and students from across the State unparalleled computing power in artificial intelligence (AI) to apply across many areas to improve lives, bolster industry, and create ec...

New Approach Isolating Stem Cells Could Lead to Breakthrough In Regenerative Medicine

A recent study conducted at the University of Florida could transform the way we isolate stem cells, validating a method that takes only minutes and can be performed just about anywhere. Stem cells are “immature” cells that have the unique ability to develop into other cell types. For example, hematopoietic stem cells (HSCs) regenerate blood cells and mesenchymal stem cells (MSCs) regenerate cells of solid tissues. Because HSCs and MSCs are often used for clinical regenerative medicine, isolati...

Instruments

Staff

Name	Title	Telephone	E-mail	Room
Sixue Chen	Proteomics & Mass Spectrometry Faculty Director	(352) 273-8057	schen@ufl.edu	169A CGRC
Jin Koh	Proteomics & Mass Spectrometry Scientific Director	(352) 273-8059	jinkoh@ufl.edu	168A CGRC
Ran Zheng	Proteomics & Mass Spectrometry Core Research Specialist I	(352) 273-8061	ranzheng@ufl.edu	169 CGRC
Ning Zhu	Proteomics & Mass Spectrometry Core Research Specialist I	(352) 273-8067	nzhu@ufl.edu	169 CGRC
Fanchao Zhu	Proteomics & Mass Spectrometry Core Research Technician III	(352) 273-8060	fzhu9482@ufl.edu	169 CGRC