Proteomics & Mass Spectrometry

OVERVIEW

ICBR Proteomics & Mass Spectrometry Facility has provided unprecedented tools for fast, accurate, high throughput biomolecular separation and characterization, which are indispensable towards understanding the biological and medical systems. Studying at the protein level allows researchers to investigate how proteins, their dynamics, modifications, and interactions affect cellular processes and how cellular processes and environment affect proteins. The mission of our facility is to provide excellent service and training in proteomics and mass spectrometry. Here we present our capabilities in proteomics, highlighting differential proteomics, which include two-dimensional difference gel electrophoresis (2D-DIGE), isobaric tags for relative and absolute quantitation (iTRAQ), tandem mass tags (TMT), iodoTMT, Stable isotope labeling with amino acids in cell culture (SILAC), quantification of proteins by label-free LC-MS/MS, and the analysis of protein post-translational modifications (PTM).

We have a suite of state-of-the-art mass spectrometers and software available for detailed characterization of proteins and peptides, including post-translational modification analysis and accurate molecular weight determination. To ensure success and maximize productivity, the Proteomics & Mass Spectrometry core offers education, consultation, data processing and reporting, and support of grant application.

CORE NEWS

UF ICBR Closed for Memorial Day

UF ICBR Closed for Memorial Day UF ICBR will be closed on Monday, May 29th in observation of Memorial Day. Normal business hours will resume on Tuesday, May 30th. No sample or packages will be accepted during this time. ...

Important Dates and Submission Deadlines for Fiscal Year End

Please note: Due to the University of Florida Fiscal Year End, the last invoices to process for FY2023 will be created on Monday, June 12th. ICBR’s upcoming billing schedule will be altered as follows: 6/12/2023 – Last invoices generated in fiscal year 2023 6/19/2023 at noon – Deadline to submit disputes for FY2023 payment. 7/3//2023 – Invoices will begin to be created for FY2023 If you have expiring funds, please work with your core laboratory contact to make sure your project is c...

ICBR’s Dr. Anna-Lisa Paul Honored as Lifetime Fellow by the AAAS

ICBR's Dr. Anna-Lisa Paul Honored as Lifetime Fellow by the AAAS

The American Association for the Advancement of Science honors 19 UF faculty as Lifetime Fellows Dr. Anna-Lisa Paul, alongside 18 other UF faculty, has been honored as a Lifetime Fellow of AAAS. Dr. Paul was honored for “distinguished contributions to the understanding of life’s ability to move beyond planet Earth, as well as extraordinary service to the space biology science community”. Her research focuses on how plants respond to environmental change, with emphasis on spaceflight...

Surviving Space: Extreme Plant Adaptation

Which plants have the best chances for survival in space? That’s the question being addressed by Principal Investigators Anna-Lisa Paul and Robert Ferl in the Epigenetic Adaptation to the Spaceflight Environment – Accumulated Genomic Change Induced by Generations in Space (Plant Habitat-03) investigation which will soon launch to the International Space Station aboard Northrop Grumman’s 18th commercial resupply services mission for NASA. “One of the exciting things about space biology ...

Important Dates and Submission Deadlines for Calendar Year End

Please note: Due to the Calendar Year End, the last invoices to process for 2022 will be created on Monday, December 12th. ICBR’s upcoming billing schedule will be altered as follows: 12/12/2022 – Last invoices generated in 2022 12/19/2022 at noon – Deadline to submit disputes for 2022 payment. If you have expiring funds, please work with your core laboratory contact to make sure your project is completed and billed before the 6/13/2022 invoices are posted. Sample Submission D...

ICBR Closed over Thanksgiving Holiday

ICBR will be closed Thursday (November 24th) and Friday (November 25th) for the Thanksgiving holiday. We here at ICBR are thankful for you, and hope you enjoy the holiday! Normal business hours will resume on Monday, November 28th. ...

Scientific expedition to study human impact on Florida Everglades

Scientific expedition to study human impact on Florida Everglades A team of five that includes a female University of Florida professor as its lead scientist will embark Thursday on a dangerous trek across the Florida Everglades to assess the impact of humans on the world’s largest subtropical wilderness. The group will retrace an 1897 canoe journey that was first completed by explorer and scientist Hugh de Laussat Willoughby. Tracie Baker, an associate professor of environmental and global h...

Big Changes for ICBR Electron Microscopy Core in 2023

We are excited to announce that our EM core is going to be relocated entirely in CGRC. The move should not only offer our users additional convenience with a singular location and increased proximity to our peer cores, but the renovation will provide our users with a revamped lab space that will expand the capabilities and offerings of our EM team. We are very excited about the next steps, but there is still a lot of work left to do. EM is currently doing their best to facilitate this move while...