Proteomics & Mass Spectrometry

Home
PROTEOMICS & MASS SPECTROMETRY:     Home   |   Staff & Contact   |  Services  |   Instruments  |   Core Resources

Email: ICBR-Proteomics@ad.ufl.edu  Phone: (352) 273-8060

Overview

ICBR Proteomics & Mass Spectrometry Facility has provided unprecedented tools for fast, accurate, high throughput biomolecular separation and characterization, which are indispensable towards understanding the biological and medical systems. Studying at the protein level allows researchers to investigate how proteins, their dynamics, modifications, and interactions affect cellular processes and how cellular processes and environment affect proteins. The mission of our facility is to provide excellent service and training in proteomics and mass spectrometry. Here we present our capabilities in proteomics, highlighting differential proteomics, which include two-dimensional difference gel electrophoresis (2D-DIGE), isobaric tags for relative and absolute quantitation (iTRAQ), tandem mass tags (TMT), iodoTMT, Stable isotope labeling with amino acids in cell culture (SILAC), quantification of proteins by label-free LC-MS/MS, and the analysis of protein post-translational modifications (PTM).

We have a suite of state-of-the-art mass spectrometers and software available for detailed characterization of proteins and peptides, including post-translational modification analysis and accurate molecular weight determination. To ensure success and maximize productivity, the Proteomics & Mass Spectrometry core offers education, consultation, data processing and reporting, and support of grant application.

Core News

ICBR Bioinformatics Contributes to Landmark UFHCC Publication ICBR Bioinformatics Contributes to Landmark UFHCC Publication
ICBR Bioinformatics Contributes to Landmark UFHCC Publication
The ICBR Bioinformatics Core contributed to a high-profile paper from the cancer research laboratory of UFHCC Director Dr. Jonathan Licht, recently published in Cancer Discovery. The paper, entitled “A Mutation in Histone H2B Represents a New Class of Oncogenic Driver”, describes how mutations in core histones disrupt nucleosome stability, leading to disregulation of gene expression and acting as a novel oncogenic mechanism. As part of this study the investigators used a technique ca...
Read More
ICBR Bioinformatics Contributes to Landmark UFHCC Publication
Help ICBR Grow in Ten Quick Questions! Help ICBR Grow in Ten Quick Questions!
Help ICBR Grow in Ten Quick Questions!
We want to hear about your experience working with UF ICBR. What do you like? How can we improve? We want to hear from you. For your convenience, we kept our survey to ten quick questions. This is your opportunity to help us continue our quest to become the best resource on campus. Click Here to Participate in our Survey ...
Read More
Help ICBR Grow in Ten Quick Questions!
Instrument Spotlight: Protein Simple Wes Instrument Spotlight: Protein Simple Wes
Instrument Spotlight: Protein Simple Wes
Wes™ lets you separate and analyze proteins by size from 2-440 kDa. He also gets you down to pg-level sensitivity with just 3 µL of starting material. Got a lot of samples and no time? No problem. Wes runs up to 25 samples in 3 hours flat and gives you quantitative, size-based data including total protein. Don’t wait to discover, get going today with Wes! Our Simple Western systems offer the right throughput for your needs. Whether you are looking to process up to 24 samples per run or ...
Read More
Instrument Spotlight: Protein Simple Wes
Instrument Spotlight: PacBio Sequel Instrument Spotlight: PacBio Sequel
Instrument Spotlight: PacBio Sequel
GAIN A COMPREHENSIVE VIEW OF GENOMES, TRANSCRIPTOMES, AND EPIGENOMES – Create high-quality whole genome de novo assemblies of eukaryotic organisms – Survey large population cohorts and resolve structural variants – Read full-length transcripts to characterize isoform diversity – Target all variant types across relevant genomic regions – Detect epigenetic modifications simultaneously Get Started: Contact ICBR NextGen DNA Sequencing Click here to see SEQUEL Services &...
Read More
Instrument Spotlight: PacBio Sequel
ICBR Closed: September 2nd and September 3rd ICBR Closed: September 2nd and September 3rd
ICBR Closed: September 2nd and September 3rd
ICBR will be closed on Monday, September 2nd in observation of Labor Day, and on Tuesday, September 3rd due to Hurricane Dorian. There is a chance we will be closed on Wednesday; please confirm our availability via phone or email. ...
Read More
ICBR Closed: September 2nd and September 3rd
Single Cell Genomics on the 10X Genomics Chromium System Single Cell Genomics on the 10X Genomics Chromium System
Single Cell Genomics on the 10X Genomics Chromium System
The ICBR and UFHCC have partnered in the acquisition of new cutting-edge technology with the capability of analyzing gene structure and gene activity at the single cell level. The Chromium instrument developed by California-based 10X Genomics inc. provides two unique key technologies to the UF research community. In one application, the instrument uses synthetic gel beads to partition thousands of separate enzymatic reactions necessary for downstream DNA sequencing. In another application, short...
Read More
Single Cell Genomics on the 10X Genomics Chromium System
CRISPR at UF ICBR CRISPR at UF ICBR
CRISPR at UF ICBR
In collaboration with the UFHCC, ICBR is pleased to announce several new CRISPR genome editing services now available to the UF research community. The CRISPR/Cas9 system allows researchers to easily introduce deletions, insertions and edited DNA sequences into mammalian cell genomes. It can be used to introduce very specific surgical edits to any gene and can in fact introduce foreign genes into specific regions of the animal cell genome. At the University of Florida, CRISPR is used within re...
Read More
CRISPR at UF ICBR
Is Your Monoclonal Antibody Authentic? Confirming Hybridoma Cell Line Identity by IgC cDNA Sequencing Is Your Monoclonal Antibody Authentic? Confirming Hybridoma Cell Line Identity by IgC cDNA Sequencing
Is Your Monoclonal Antibody Authentic? Confirming Hybridoma Cell Line Identity by IgC cDNA Sequencing
Widespread reports of irreproducibility in health sciences research has prompted the NIH to update new grant application guidelines to include a brief description of methods that will be used to ensure identity and validity of key biological and/or chemical resources used in the proposed study. In this regard there is a significant effort now underway using DNA “fingerprinting” as a way to use microsatellite repeat lengths as a powerful tool for human cell line authentication. This method ha...
Read More
Is Your Monoclonal Antibody Authentic? Confirming Hybridoma Cell Line Identity by IgC cDNA Sequencing

Instruments

Staff

NameTitleTelephoneE-mailRoom
Sixue ChenProteomics & Mass Spectrometry Faculty Director(352) 273-8057schen@ad.ufl.edu169A CGRC
Jin KohProteomics & Mass Spectrometry Scientific Director(352) 273-8059jinkoh@ufl.edu168A CGRC
Tingting WangProteomics & Mass Spectrometry Assistant Scientist(352) 273-8060wangt1@ufl.edu168 CGRC
Ran ZhengProteomics & Mass Spectrometry Core Research Specialist I(352) 273-8061ranzheng@ufl.edu169 CGRC
Ning ZhuProteomics & Mass Spectrometry Core Research Specialist I(352) 273-8067nzhu@ufl.edu169 CGRC
Fanchao ZhuProteomics & Mass Spectrometry Core Research Technician III(352) 273-8060fzhu9482@ufl.edu169 CGRC