Proteomics & Mass Spectrometry

OVERVIEW

ICBR Proteomics & Mass Spectrometry Facility has provided unprecedented tools for fast, accurate, high throughput biomolecular separation and characterization, which are indispensable towards understanding the biological and medical systems. Studying at the protein level allows researchers to investigate how proteins, their dynamics, modifications, and interactions affect cellular processes and how cellular processes and environment affect proteins. The mission of our facility is to provide excellent service and training in proteomics and mass spectrometry. Here we present our capabilities in proteomics, highlighting differential proteomics, which include two-dimensional difference gel electrophoresis (2D-DIGE), isobaric tags for relative and absolute quantitation (iTRAQ), tandem mass tags (TMT), iodoTMT, Stable isotope labeling with amino acids in cell culture (SILAC), quantification of proteins by label-free LC-MS/MS, and the analysis of protein post-translational modifications (PTM).

We have a suite of state-of-the-art mass spectrometers and software available for detailed characterization of proteins and peptides, including post-translational modification analysis and accurate molecular weight determination. To ensure success and maximize productivity, the Proteomics & Mass Spectrometry core offers education, consultation, data processing and reporting, and support of grant application.

CORE NEWS

COVID Updates for UF | ICBR

Dear ICBR colleagues and customers, The COVID19 Delta Variant is causing many of the concerns we all had in the early stages of the pandemic, and as a consequence, there is a recognition that we as a community can help mitigate the spread by also returning to some of the precautions we adopted last year. Because ICBR and UF Health share work spaces, we will adopt the same masking policy that is currently being implemented within Shands/UF Health. This practice means that everyone within ICBR pub...

UF ICBR Launches New Self-Service Timer

UF | ICBR has launched their latest version of our self-service timer. Please see below, a guide on how the new timer can benefit your experience. Accessing the Application To access the application, follow these steps: Navigate to selfservice.biotech.ufl.edu Click on Access the Self Service Suite button Authenticate with your GatorLink credentials Click on Timer Browsing the Cores To browse the list of cores, follow these steps: On the navigation bar on top of the screen, click on C...

UF Researcher Dr. Marco Salemi Utilizes ICBR Genomics Expertise to Understand COVID19 Epidemiology

By Steven Madore, Ph.D. In recent years, public health emergencies caused by epidemics have led to the use of genome sequencing to identify and characterize viral pathogens. Rapid acquisition of high quality viral genomic sequences is critical for understanding viral pathogen origin, transmission and epidemiological spread. Ultimately deciphering the molecular characteristics of viruses accelerates the development of diagnostic assays and vaccine development and drug design and is important in u...

UF researchers use AI datasets to track feral pigs, minimize disease risk

Courtesy of UF/IFAS Written by Brad Buck Feral pigs cost the agriculture industry at least $1.5 billion in damage, disease and control costs around the United States annually, running rampant on large swaths of grazing lands. The swine root in soil and eat most everything in sight. Farmers and ranchers will benefit from research by University of Florida scientists who are using artificial intelligence to gather data on feral hog reproduction and movement. As they roam grazing land, feral swine ...

UF | ICBR Scientists Contribute to Clinical Trial Testing Novel Treatment for Canine Atopic Dermatitis

Atopic dermatitis, or AD, is a common chronic inflammatory skin disease affecting dogs, causing defects of the skin barrier and modifications of the normal cutaneous microflora. While multiple treatment options are available, there is a growing interest in the use of alternative natural ingredient products. In human AD reports show the potential benefits of topical applications of heat-killed beneficial bacteria, with some studies demonstrating the efficacy of rapidly killed beneficial la...

UF Researchers to Use AI to Predict How Hurricanes Spread Invasive Plants

Courtesy of UF/IFAS Written by Brad Buck Scientists project hurricane intensity and frequency will increase with climate change. That leads researchers to want to better predict how storms will disperse and establish nonnative plant invaders. Knowing where invasive plants spread will always be useful. But it may become especially handy soon as the National Hurricane Center is considering moving the official start of the Atlantic hurricane season two weeks earlier, starting it May 15, instead of ...

World-Renowned UF Health Biochemistry Researcher Passes Away

Courtesy of College of Medicine News University of Florida Health biochemistry professor Mavis Agbandje-McKenna, Ph.D., whose world-renowned work on the detailed structure of viruses led to advances in gene therapy treatments for different diseases, died Wednesday at her home near Gainesville of the neurodegenerative disease amyotrophic lateral sclerosis. She was 57. Agbandje-McKenna’s work with the small, infectious particles had a worldwide impact, allowing other scientists to be more precise ...

UF Health Virologist Talks about Variants and the Future of the Coronavirus

Courtesy of UF Health Written by Bill Levesque The novel coronavirus is doing what viruses do — in fact, what all life does. It’s mutating and creating new variants. This is evolution 101. Much of the discussion about the coronavirus in recent weeks has been about the potential impact of these variants on COVID-19 vaccines now being administered worldwide. Just what does the future hold? University of Florida Health virologist John Lednicky, Ph.D., a professor in the in the UF College of Public ...