Proteomics & Mass Spectrometry

ICBR-Proteomics@ad.ufl.edu   ///  (352) 273-8060  ///  RRID:SCR_019151
Gel Electrophoresis, High Throughput Separation, Protein Quantification and More

OVERVIEW

ICBR Proteomics & Mass Spectrometry Facility has provided unprecedented tools for fast, accurate, high throughput biomolecular separation and characterization, which are indispensable towards understanding the biological and medical systems. Studying at the protein level allows researchers to investigate how proteins, their dynamics, modifications, and interactions affect cellular processes and how cellular processes and environment affect proteins. The mission of our facility is to provide excellent service and training in proteomics and mass spectrometry. Here we present our capabilities in proteomics, highlighting differential proteomics, which include two-dimensional difference gel electrophoresis (2D-DIGE), isobaric tags for relative and absolute quantitation (iTRAQ), tandem mass tags (TMT), iodoTMT, Stable isotope labeling with amino acids in cell culture (SILAC), quantification of proteins by label-free LC-MS/MS, and the analysis of protein post-translational modifications (PTM).

We have a suite of state-of-the-art mass spectrometers and software available for detailed characterization of proteins and peptides, including post-translational modification analysis and accurate molecular weight determination. To ensure success and maximize productivity, the Proteomics & Mass Spectrometry core offers education, consultation, data processing and reporting, and support of grant application.

CORE NEWS

Surviving Space: Extreme Plant Adaptation Surviving Space: Extreme Plant Adaptation
Surviving Space: Extreme Plant Adaptation
Which plants have the best chances for survival in space? That’s the question being addressed by Principal Investigators Anna-Lisa Paul and Robert Ferl in the Epigenetic Adaptation to the Spaceflight Environment – Accumulated Genomic Change Induced by Generations in Space (Plant Habitat-03) investigation which will soon launch to the International Space Station aboard Northrop Grumman’s 18th commercial resupply services mission for NASA. “One of the exciting things about space biology ...
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Surviving Space: Extreme Plant Adaptation
UF ICBR Closed for Martin Luther King, Jr. Day UF ICBR Closed for Martin Luther King, Jr. Day
UF ICBR Closed for Martin Luther King, Jr. Day
UF ICBR Closed for Martin Luther King, Jr. Day UF ICBR will be closed on Monday, January 16th in observation of Martin Luther King, Jr. Day. Normal hours will resume on Tuesday, January 17th. No packages will be accepted during this time! ...
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UF ICBR Closed for Martin Luther King, Jr. Day
ICBR Closed for the Holidays! ICBR Closed for the Holidays!
ICBR Closed for the Holidays!
Wishing you and yours a Happy Holiday and a joyful New Year. Best wishes from your friends at UF | ICBR! ICBR will be closed Monday (December 26th), returning on Tuesday (January 3rd). We here at ICBR are thankful for you, and hope you enjoy the holiday! And a special thank you the near thousand users that came through our doors this year, including 300+ PIs from over a hundred UF departments! We look forward to working with you 2023!     Normal business hours will resume on Tuesday, J...
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ICBR Closed for the Holidays!
Important Dates and Submission Deadlines for Calendar Year End Important Dates and Submission Deadlines for Calendar Year End
Important Dates and Submission Deadlines for Calendar Year End
Please note: Due to the Calendar Year End, the last invoices to process for 2022 will be created on Monday, December 12th. ICBR’s upcoming billing schedule will be altered as follows: 12/12/2022 – Last invoices generated in 2022 12/19/2022 at noon – Deadline to submit disputes for 2022 payment. If you have expiring funds, please work with your core laboratory contact to make sure your project is completed and billed before the 6/13/2022 invoices are posted.   Sample Submission D...
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Important Dates and Submission Deadlines for Calendar Year End
ICBR Closed over Thanksgiving Holiday ICBR Closed over Thanksgiving Holiday
ICBR Closed over Thanksgiving Holiday
ICBR will be closed Thursday (November 24th) and Friday (November 25th) for the Thanksgiving holiday. We here at ICBR are thankful for you, and hope you enjoy the holiday!   Normal business hours will resume on Monday, November 28th.     ...
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ICBR Closed over Thanksgiving Holiday
Scientific expedition to study human impact on Florida Everglades Scientific expedition to study human impact on Florida Everglades
Scientific expedition to study human impact on Florida Everglades
Scientific expedition to study human impact on Florida Everglades A team of five that includes a female University of Florida professor as its lead scientist will embark Thursday on a dangerous trek across the Florida Everglades to assess the impact of humans on the world’s largest subtropical wilderness. The group will retrace an 1897 canoe journey that was first completed by explorer and scientist Hugh de Laussat Willoughby. Tracie Baker, an associate professor of environmental and global h...
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Scientific expedition to study human impact on Florida Everglades
Big Changes for ICBR Electron Microscopy Core in 2023 Big Changes for ICBR Electron Microscopy Core in 2023
Big Changes for ICBR Electron Microscopy Core in 2023
We are excited to announce that our EM core is going to be relocated entirely in CGRC. The move should not only offer our users additional convenience with a singular location and increased proximity to our peer cores, but the renovation will provide our users with a revamped lab space that will expand the capabilities and offerings of our EM team. We are very excited about the next steps, but there is still a lot of work left to do. EM is currently doing their best to facilitate this move while...
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Big Changes for ICBR Electron Microscopy Core in 2023
ICBR Announces Special Pilot Grant Opportunity ICBR Announces Special Pilot Grant Opportunity
ICBR Announces Special Pilot Grant Opportunity
DEADLINE EXTENDED: ICBR Announces Special Pilot Grant Opportunity The mission of ICBR is to champion and enable scientific discovery and innovation throughout the University of Florida research community by providing access to world-class biotechnology expertise and instrumentation. To introduce the UF research community to the use of Oxford Nanopore Technology PromethION nanopore DNA sequencing, the ICBR is requesting pilot project proposals utilizing this technology for funding consideration. ...
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ICBR Announces Special Pilot Grant Opportunity

SELECT INSTRUMENTS

Proteomics & Mass Spectrometry