Proteomics & Mass Spectrometry

ICBR-Proteomics@ad.ufl.edu   ///  (352) 273-8060  ///  RRID:SCR_019151
Gel Electrophoresis, High Throughput Separation, Protein Quantification and More

OVERVIEW

ICBR Proteomics & Mass Spectrometry Facility has provided unprecedented tools for fast, accurate, high throughput biomolecular separation and characterization, which are indispensable towards understanding the biological and medical systems. Studying at the protein level allows researchers to investigate how proteins, their dynamics, modifications, and interactions affect cellular processes and how cellular processes and environment affect proteins. The mission of our facility is to provide excellent service and training in proteomics and mass spectrometry. Here we present our capabilities in proteomics, highlighting differential proteomics, which include two-dimensional difference gel electrophoresis (2D-DIGE), isobaric tags for relative and absolute quantitation (iTRAQ), tandem mass tags (TMT), iodoTMT, Stable isotope labeling with amino acids in cell culture (SILAC), quantification of proteins by label-free LC-MS/MS, and the analysis of protein post-translational modifications (PTM).

We have a suite of state-of-the-art mass spectrometers and software available for detailed characterization of proteins and peptides, including post-translational modification analysis and accurate molecular weight determination. To ensure success and maximize productivity, the Proteomics & Mass Spectrometry core offers education, consultation, data processing and reporting, and support of grant application.

CORE NEWS

ICBR Closed Friday (7/18) for Team Building ICBR Closed Friday (7/18) for Team Building
ICBR Closed Friday (7/18) for Team Building
ICBR Closed Friday for a Strategic Retreat ICBR will be closed from 8am-5pm on Friday (7/18) for a special All Staff Strategic Retreat. We will not be accepting packages or samples on Friday. Our normal business hours will resume on Monday. Please refer to our staff directory for additional details. ...
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ICBR Closed Friday (7/18) for Team Building
ICBR Closed Friday for the 4th of July ICBR Closed Friday for the 4th of July
ICBR Closed Friday for the 4th of July
ICBR Closed Friday for the 4th of July ICBR will be closed this Friday (7/4) in observation of the Fourth of July   We will not be accepting packages or samples on Friday. Our normal business hours will resume on Monday (7/7). Enjoy the the long weekend!   Please refer to our staff directory for additional details. ...
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ICBR Closed Friday for the 4th of July
Environmental DNA – It’s All Up in the Air Environmental DNA – It’s All Up in the Air
Environmental DNA – It’s All Up in the Air
Environmental DNA – It’s All Up in the Air Steven J. Madore, PhD (ICBR Associate Director for Science) (Note: this article has been summarized from UF News “Environmental DNA floating in the air tracks wildlife, viruses — even drugs” June 3, 2025 by Eric Hamilton) Scientists can now use the power of genetic information to identify biodiversity by sampling environmental DNA or eDNA. “The level of information that’s available in environmental DNA is such that we’re only starting to consider what t...
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Environmental DNA – It’s All Up in the Air
ICBR Closed Thursday for Juneteenth ICBR Closed Thursday for Juneteenth
ICBR Closed Thursday for Juneteenth
ICBR Closed Thursday for Juneteenth ICBR will be closed this Thursday (6/19) in observation of Juneteenth   We will not be accepting packages or samples on Thursday. Our normal business hours will resume on Friday (6/20).   Please refer to our staff directory for additional details. ...
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ICBR Closed Thursday for Juneteenth
UF Investigators Identify Mechanism of Drug Resistance in Non-Small Cell Lung Cancer UF Investigators Identify Mechanism of Drug Resistance in Non-Small Cell Lung Cancer
UF Investigators Identify Mechanism of Drug Resistance in Non-Small Cell Lung Cancer
UF Investigators Identify Mechanism of Drug Resistance in Non-Small Cell Lung Cancer Steven J Madore, PhD (ICBR Associate Director for Science) Summarized from J. Clin. Inv. 2025;135(6); e185149 Non-small cell lung cancer (NSCLC) is a leading cause of cancer-related deaths worldwide. Despite a combination of surgery and chemotherapy, survival rate remains low due to cancer cell metastasis and drug resistance.  Mutations in the oncogene KRAS are frequently found in human cancers, including up to ...
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UF Investigators Identify Mechanism of Drug Resistance in Non-Small Cell Lung Cancer
Stars Align for Science: University of Florida and NSF NRAO Forge Path for Discovery Stars Align for Science: University of Florida and NSF NRAO Forge Path for Discovery
Stars Align for Science: University of Florida and NSF NRAO Forge Path for Discovery
Stars Align for Science: University of Florida and NSF NRAO Forge Path for Discovery The U.S. National Science Foundation National Radio Astronomy Observatory (NSF NRAO) and the University of Florida (UF) have announced a dynamic new partnership to advance science education and public outreach at a future Next Generation Very Large Array (ngVLA) antenna site in Florida. This collaboration, signed with a Memorandum of Understanding (MOU) at the debut of the ngVLA prototype antenna in New Mexico l...
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Stars Align for Science: University of Florida and NSF NRAO Forge Path for Discovery
Florida Trend: Seeding Exploration Florida Trend: Seeding Exploration
Florida Trend: Seeding Exploration
Seeding Exploration Twelve years to get 12 grams. Twelve years of research and planning, of conducting experiments with simulated material, of traveling to the world’s most inhospitable environments. Twelve years of writing proposals and being told no. University of Florida research scientists Rob Ferl and Anna-Lisa Paul weren’t asking NASA for just 12 grams oflunar regolith – basically dust and dirt from the moon – but that was the payoff in 2022 for their perseverance. ...
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Florida Trend: Seeding Exploration
Sony ID7000 Spectral Cytometer at UF ICBR Sony ID7000 Spectral Cytometer at UF ICBR
Sony ID7000 Spectral Cytometer at UF ICBR
Sony ID7000 Spectral Cytometer at UF ICBR The Cytometry & Optical Microscopy core at UF ICBR has recently acquired the state-of-the-art Sony ID7000 Spectral Cytometer, located at McKnight Brain Institute (MBI) room L3-160. This newest addition to the facility offers unparalleled capabilities for researchers, significantly expanding their experimental possibilities. The Sony ID7000 features an impressive configuration of five lasers and 144 detectors, enabling experiments utilizing over 40 fl...
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Sony ID7000 Spectral Cytometer at UF ICBR

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Proteomics & Mass Spectrometry