Proteomics & Mass Spectrometry

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PROTEOMICS & MASS SPECTROMETRY:     Home   |   Staff & Contact   |  Services  |   Instruments  |   Core Resources

Email: ICBR-Proteomics@ad.ufl.edu  Phone: (352) 273-8060

Overview

ICBR Proteomics & Mass Spectrometry Facility has provided unprecedented tools for fast, accurate, high throughput biomolecular separation and characterization, which are indispensable towards understanding the biological and medical systems. Studying at the protein level allows researchers to investigate how proteins, their dynamics, modifications, and interactions affect cellular processes and how cellular processes and environment affect proteins. The mission of our facility is to provide excellent service and training in proteomics and mass spectrometry. Here we present our capabilities in proteomics, highlighting differential proteomics, which include two-dimensional difference gel electrophoresis (2D-DIGE), isobaric tags for relative and absolute quantitation (iTRAQ), tandem mass tags (TMT), iodoTMT, Stable isotope labeling with amino acids in cell culture (SILAC), quantification of proteins by label-free LC-MS/MS, and the analysis of protein post-translational modifications (PTM).

We have a suite of state-of-the-art mass spectrometers and software available for detailed characterization of proteins and peptides, including post-translational modification analysis and accurate molecular weight determination. To ensure success and maximize productivity, the Proteomics & Mass Spectrometry core offers education, consultation, data processing and reporting, and support of grant application.

Core News

BI assistant scientist develops a tool for stratifying cancer patients BI assistant scientist develops a tool for stratifying cancer patients
BI assistant scientist develops a tool for stratifying cancer patients
Bioinformatics assistant scientist Tongjun Gu Ph.D. has developed an advanced deep learning method to stratify cancer patients into clinically relevant subgroups and further identify the potential biomarkers between the subgroups. This will help with the development of personalized treatments and prognosis. The method can integrate multi-platform datasets like gene expression, miRNA expression, methylation, copy number alteration, and protein expression. It can also do transfer learning between ...
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BI assistant scientist develops a tool for stratifying cancer patients
ICBR Proteomics & Mass Spectrometry (PM) aims to bring single-cell proteomics and metabolomics technologies to customers ICBR Proteomics & Mass Spectrometry (PM) aims to bring single-cell proteomics and metabolomics technologies to customers
ICBR Proteomics & Mass Spectrometry (PM) aims to bring single-cell proteomics and metabolomics technologies to customers
The cell is the basic unit of life. For multicellular organisms like animals and plants, each cell is different. Due to limited sensitivity, conventional ‘omic analyses require the input of tissues, organs or a mixture of thousands of cells, where information at the single-cell level has been diluted and averaged. This problem has severely limited our understanding of the intricate signaling and metabolic pathways and networks in different cells. In recent years, single-cell biology has become...
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ICBR Proteomics & Mass Spectrometry (PM) aims to bring single-cell proteomics and metabolomics technologies to customers
Important Upcoming Billing Dates Important Upcoming Billing Dates
Important Upcoming Billing Dates
University of Florida and ICBR core labs will be officially closed during the UF holiday: Wednesday, December 25, through Wednesday, January 1. No samples or other packages can be accepted during this holiday closure time. Please coordinate with individual core laboratories for delivery of samples or other packages either before or after the UF winter break. Due to the winter break, ICBR’s upcoming billing schedule will be altered as follows: 12/16/2019 – Last invoices generated in 2019...
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Important Upcoming Billing Dates
A New Method to Examine Human Dopamine Transporter in Peripheral Blood A New Method to Examine Human Dopamine Transporter in Peripheral Blood
A New Method to Examine Human Dopamine Transporter in Peripheral Blood 
A team of UF neuroscientists led by Habibeh Khoshbouei, Ph.D., Pharm.D., Professor of Neuroscience and Psychiatry in the UF College of Medicine, has recently discovered a novel method using blood-borne immune cells to study the role of the neurotransmitter dopamine in the human brain.  This finding was reported in a publication titled ”A novel approach to study markers of dopamine signaling in peripheral immune cells” appearing in the Journal of Immunological Methods. While immunohistochemi...
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A New Method to Examine Human Dopamine Transporter in Peripheral Blood
Dr. Tongjun Gu, from ICBR Bioinformatics, Improves Discovery Pipeline Dr. Tongjun Gu, from ICBR Bioinformatics, Improves Discovery Pipeline
Dr. Tongjun Gu, from ICBR Bioinformatics, Improves Discovery Pipeline
Bioinformatics assistant scientist Tongjun Gu Ph.D. has improved the pipeline for discovering ncRNA editing sites from four human cancers. Published in JCO Clinical Cancer Informatics, Clinical Relevance of Noncoding Adenosine-to-Inosine RNA Editing in Multiple Human Cancers, Dr. Gu’s research also presents multiple advanced statistical methods for differential and survival analysis. More information: Bioinformatics Services Contact Bioinformatics ...
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Dr. Tongjun Gu, from ICBR Bioinformatics, Improves Discovery Pipeline
ICBR Faculty Director to Speak at 2019 Florida Genetics Symposium ICBR Faculty Director to Speak at 2019 Florida Genetics Symposium
ICBR Faculty Director to Speak at 2019 Florida Genetics Symposium
We are excited to announce that Sixue Chen, PhD will present at the 2019 Florida Genetics Symposium, presented by the University of Florida Genetics Institute. This two day event on November 4th and 5th will bring together some of the brightest minds in genetics. Faculty Director of the ICBR Proteomics & Mass Spectrometry Core, Dr. Chen will present “Single Cell-Type Proteomics and Metabolomics: New Insights into Guard Cell Umminuty and CO2 Responses” on Monday, November 4th from 1:45pm ...
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ICBR Faculty Director to Speak at 2019 Florida Genetics Symposium
The Beast is Here!  ICBR Launches the NovaSeq 6000 DNA Sequencer The Beast is Here! ICBR Launches the NovaSeq 6000 DNA Sequencer
The Beast is Here! ICBR Launches the NovaSeq 6000 DNA Sequencer
Dr. Steven Madore, ICBR Associate Director for Science and UFHCC Associate Director for Shared Resources, next to the new NovaSeq6000 (“the Beast“) with ICBR NGS Core Scientific Director Dr. David Moraga. The ICBR NextGen DNA Sequencing Core provides cutting edge instrumentation and technical expertise required to support campus-wide research activites. Established in 1987, the NGS Core has contributed to significant scientific discoveries on campus. For example, did you know that the first...
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The Beast is Here!  ICBR Launches the NovaSeq 6000 DNA Sequencer
The Luminex FLEXMAP 3D®  System – A Multiplexed Analytical Platform for  Novel Biomarker Discovery The Luminex FLEXMAP 3D® System – A Multiplexed Analytical Platform for Novel Biomarker Discovery
The Luminex FLEXMAP 3D® System – A Multiplexed Analytical Platform for Novel Biomarker Discovery
Figure 1. Luminex bead architecture technology ICBR Proteomics & Mass Spectrometry (PM) Core is now offering access to the Luminex FLEXMAP 3D®   analytical platform for discovering and validation of protein biomarkers across a wide range of diseases. The FLEXMAP 3D® system combines differentially dyed fluorescent microsphere sets with an innovative instrument design to enable simultaneous measurement of  the concentration of a large number of different analytes in a rapid multiplexed as...
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The Luminex FLEXMAP 3D®  System – A Multiplexed Analytical Platform for  Novel Biomarker Discovery

Instruments

Staff

NameTitleTelephoneE-mailRoom
Sixue Chen, Ph.D.Proteomics & Mass Spectrometry Faculty Director(352) 273-8057schen@ufl.edu169A CGRC
Jin Koh, Ph.D.Proteomics & Mass Spectrometry Scientific Director(352) 273-8059jinkoh@ufl.edu168A CGRC
Tingting Wang, Ph.D.Proteomics & Mass Spectrometry Assistant Scientist(352) 273-8060wangt1@ufl.edu168 CGRC
Ran Zheng, M.S.Proteomics & Mass Spectrometry Core Research Specialist I(352) 273-8061ranzheng@ufl.edu169 CGRC
Ning Zhu, B.S.Proteomics & Mass Spectrometry Core Research Specialist I(352) 273-8067nzhu@ufl.edu169 CGRC
Fanchao Zhu, B.S.Proteomics & Mass Spectrometry Core Research Technician III(352) 273-8060fzhu9482@ufl.edu169 CGRC