Proteomics & Mass Spectrometry

OVERVIEW

ICBR Proteomics & Mass Spectrometry Facility has provided unprecedented tools for fast, accurate, high throughput biomolecular separation and characterization, which are indispensable towards understanding the biological and medical systems. Studying at the protein level allows researchers to investigate how proteins, their dynamics, modifications, and interactions affect cellular processes and how cellular processes and environment affect proteins. The mission of our facility is to provide excellent service and training in proteomics and mass spectrometry. Here we present our capabilities in proteomics, highlighting differential proteomics, which include two-dimensional difference gel electrophoresis (2D-DIGE), isobaric tags for relative and absolute quantitation (iTRAQ), tandem mass tags (TMT), iodoTMT, Stable isotope labeling with amino acids in cell culture (SILAC), quantification of proteins by label-free LC-MS/MS, and the analysis of protein post-translational modifications (PTM).

We have a suite of state-of-the-art mass spectrometers and software available for detailed characterization of proteins and peptides, including post-translational modification analysis and accurate molecular weight determination. To ensure success and maximize productivity, the Proteomics & Mass Spectrometry core offers education, consultation, data processing and reporting, and support of grant application.

CORE NEWS

Dr. David Moraga: 30 Years of Advancing Scientific Excellence at ICBR

Dr. David Moraga: 30 Years of Advancing Scientific Excellence at ICBR David Moraga, PhD, the NextGen Sequencing director at the ICBR, is retiring after 30 years of service. Since 1993, Dr. Moraga has played a vital role in advancing the center’s mission of providing cutting-edge scientific technology and research support services to the global scientific community. “Dr. David Moraga’s name has been almost synonymous with DNA sequencing for decades at ICBR and in the UF biotech communi...

Labor Day Notice: ICBR Closed on September 4th

Labor Day Notice: ICBR Closed on September 4th Please note that the ICBR will be closed on September 4th in observance of Labor Day. Kindly be aware that we will not be able to accept any packages or samples during this time. We will resume regular operations on the following business day. Please refer to our staff directory for additional details. ...

ICBR Closed Wednesday, August 30th for Tropical Storm Idalia

ICBR Closed Wednesday, August 30th for Tropical Storm Idalia Due to Tropical Storm Idalia, the ICBR will close beginning at noon on (8/29) through Wednesday (8/30). We will not be accepting packages or samples during this time. The university expects to make an announcement on Wednesday regarding the resumption of normal campus operations. Please visit www.ufl.edu for updates and information on storm preparation. Please refer to our staff directory for additional details. ...

Dr. Alberto Riva at ISMB/ECCB2023: Advancing Bioinformatics Cores and Inspiring Collaboration

Audience at the BioInfo Core Session at ISMB 2023 Dr. Alberto Riva at ISMB/ECCB2023: Advancing Bioinformatics Cores and Inspiring Collaboration Dr. Alberto Riva, the Scientific Director for the ICBR Bioinformatics core, recently attended the highly acclaimed Intelligent Systems for Molecular Biology (ISMB) conference, renowned as the largest and most prestigious gathering in the field of computational biology and bioinformatics. This year’s conference, held in Lyon, France in partnership w...

ICBR’s Dr. Anna-Lisa Paul Honored as Lifetime Fellow by the AAAS

ICBR's Dr. Anna-Lisa Paul Honored as Lifetime Fellow by the AAAS

The American Association for the Advancement of Science honors 19 UF faculty as Lifetime Fellows Dr. Anna-Lisa Paul, alongside 18 other UF faculty, has been honored as a Lifetime Fellow of AAAS. Dr. Paul was honored for “distinguished contributions to the understanding of life’s ability to move beyond planet Earth, as well as extraordinary service to the space biology science community”. Her research focuses on how plants respond to environmental change, with emphasis on spaceflight...

Surviving Space: Extreme Plant Adaptation

Which plants have the best chances for survival in space? That’s the question being addressed by Principal Investigators Anna-Lisa Paul and Robert Ferl in the Epigenetic Adaptation to the Spaceflight Environment – Accumulated Genomic Change Induced by Generations in Space (Plant Habitat-03) investigation which will soon launch to the International Space Station aboard Northrop Grumman’s 18th commercial resupply services mission for NASA. “One of the exciting things about space biology ...

Scientific expedition to study human impact on Florida Everglades

Scientific expedition to study human impact on Florida Everglades A team of five that includes a female University of Florida professor as its lead scientist will embark Thursday on a dangerous trek across the Florida Everglades to assess the impact of humans on the world’s largest subtropical wilderness. The group will retrace an 1897 canoe journey that was first completed by explorer and scientist Hugh de Laussat Willoughby. Tracie Baker, an associate professor of environmental and global h...

Big Changes for ICBR Electron Microscopy Core in 2023

We are excited to announce that our EM core is going to be relocated entirely in CGRC. The move should not only offer our users additional convenience with a singular location and increased proximity to our peer cores, but the renovation will provide our users with a revamped lab space that will expand the capabilities and offerings of our EM team. We are very excited about the next steps, but there is still a lot of work left to do. EM is currently doing their best to facilitate this move while...