Proteomics & Mass Spectrometry

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PROTEOMICS & MASS SPECTROMETRY:     Home   |   Staff & Contact   |  Services  |   Instruments  |   Core Resources

Email: ICBR-Proteomics@ad.ufl.edu  Phone: (352) 273-8060

Overview

ICBR Proteomics & Mass Spectrometry Facility has provided unprecedented tools for fast, accurate, high throughput biomolecular separation and characterization, which are indispensable towards understanding the biological and medical systems. Studying at the protein level allows researchers to investigate how proteins, their dynamics, modifications, and interactions affect cellular processes and how cellular processes and environment affect proteins. The mission of our facility is to provide excellent service and training in proteomics and mass spectrometry. Here we present our capabilities in proteomics, highlighting differential proteomics, which include two-dimensional difference gel electrophoresis (2D-DIGE), isobaric tags for relative and absolute quantitation (iTRAQ), tandem mass tags (TMT), iodoTMT, Stable isotope labeling with amino acids in cell culture (SILAC), quantification of proteins by label-free LC-MS/MS, and the analysis of protein post-translational modifications (PTM).

We have a suite of state-of-the-art mass spectrometers and software available for detailed characterization of proteins and peptides, including post-translational modification analysis and accurate molecular weight determination. To ensure success and maximize productivity, the Proteomics & Mass Spectrometry core offers education, consultation, data processing and reporting, and support of grant application.

Core News

ICBR Faculty Director to Speak at 2019 Florida Genetics Symposium ICBR Faculty Director to Speak at 2019 Florida Genetics Symposium
ICBR Faculty Director to Speak at 2019 Florida Genetics Symposium
We are excited to announce that Sixue Chen, PhD will present at the 2019 Florida Genetics Symposium, presented by the University of Florida Genetics Institute. This two day event on November 4th and 5th will bring together some of the brightest minds in genetics. Faculty Director of the ICBR Proteomics & Mass Spectrometry Core, Dr. Chen will present “Single Cell-Type Proteomics and Metabolomics: New Insights into Guard Cell Umminuty and CO2 Responses” on Monday, November 4th from 1:45pm ...
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ICBR Faculty Director to Speak at 2019 Florida Genetics Symposium
The Beast is Here!  ICBR Launches the NovaSeq 6000 DNA Sequencer The Beast is Here! ICBR Launches the NovaSeq 6000 DNA Sequencer
The Beast is Here! ICBR Launches the NovaSeq 6000 DNA Sequencer
Dr. Steven Madore, ICBR Associate Director for Science and UFHCC Associate Director for Shared Resources, next to the new NovaSeq6000 (“the Beast“) with ICBR NGS Core Scientific Director Dr. David Moraga. The ICBR NextGen DNA Sequencing Core provides cutting edge instrumentation and technical expertise required to support campus-wide research activites. Established in 1987, the NGS Core has contributed to significant scientific discoveries on campus. For example, did you know that the first...
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The Beast is Here!  ICBR Launches the NovaSeq 6000 DNA Sequencer
The Luminex FLEXMAP 3D®  System – A Multiplexed Analytical Platform for  Novel Biomarker Discovery The Luminex FLEXMAP 3D® System – A Multiplexed Analytical Platform for Novel Biomarker Discovery
The Luminex FLEXMAP 3D® System – A Multiplexed Analytical Platform for Novel Biomarker Discovery
Figure 1. Luminex bead architecture technology ICBR Proteomics & Mass Spectrometry (PM) Core is now offering access to the Luminex FLEXMAP 3D®   analytical platform for discovering and validation of protein biomarkers across a wide range of diseases. The FLEXMAP 3D® system combines differentially dyed fluorescent microsphere sets with an innovative instrument design to enable simultaneous measurement of  the concentration of a large number of different analytes in a rapid multiplexed as...
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The Luminex FLEXMAP 3D®  System – A Multiplexed Analytical Platform for  Novel Biomarker Discovery
Dr. Anna-Lisa Paul receives NASA’s Exceptional Scientific Achievement Medal Dr. Anna-Lisa Paul receives NASA’s Exceptional Scientific Achievement Medal
Dr. Anna-Lisa Paul receives NASA's Exceptional Scientific Achievement Medal
Congratulations to UF’s Dr. Anna-Lisa Paul on receiving the NASA Exceptional Scientific Achievement Medal this past September! The prestigious award is reserved for individual efforts that are in line with NASA’s mission and have resulted in considerable scientific discovery or resulted in contributions of fundamental importance in their field or significantly improved the understanding of their field. Dr. Paul is the Interim Director at UF ICBR and Research Professor in the UF Horticultural...
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Dr. Anna-Lisa Paul receives NASA’s Exceptional Scientific Achievement Medal
Introducing Core Directors’ Open Consultation Meetings Introducing Core Directors’ Open Consultation Meetings
Introducing Core Directors' Open Consultation Meetings
In a continued effort to provide accessible and specialized expertise to our users, UF ICBR is proud to announce our Core Directors’ Open Consultation Meetings. Each month we will be available for a two hour walk-in session to discuss the best approaches and strategies for using ICBR technologies and expertise to drive your research forward. Our first meeting will be on Monday, October 7th from 11:00am to 1:00pm in CGRC 189. No appointment is require nor do you have to be a current ICBR user t...
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Introducing Core Directors’ Open Consultation Meetings
The NovaSeq 6000 has arrived at UF ICBR! The NovaSeq 6000 has arrived at UF ICBR!
The NovaSeq 6000 has arrived at UF ICBR!
After much anticipation, the Illumina NovaSeq 6000 has arrived at UF ICBR. Users can expect access later this fall. The NovaSeq 6000 Sequencing System unleashes groundbreaking innovations that leverage our proven technology. Now you can get scalable throughput and flexibility for virtually any sequencing method, genome, and scale of project. The NovaSeq will be housed in our NextGen DNA Sequencing core. Stay tuned for more information and events this fall. For more information on the instrument...
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The NovaSeq 6000 has arrived at UF ICBR!
ICBR Bioinformatics Contributes to Landmark UFHCC Publication ICBR Bioinformatics Contributes to Landmark UFHCC Publication
ICBR Bioinformatics Contributes to Landmark UFHCC Publication
The ICBR Bioinformatics Core contributed to a high-profile paper from the cancer research laboratory of UFHCC Director Dr. Jonathan Licht, recently published in Cancer Discovery. The paper, entitled “A Mutation in Histone H2B Represents a New Class of Oncogenic Driver”, describes how mutations in core histones disrupt nucleosome stability, leading to disregulation of gene expression and acting as a novel oncogenic mechanism. As part of this study the investigators used a technique ca...
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ICBR Bioinformatics Contributes to Landmark UFHCC Publication
Help ICBR Grow in Ten Quick Questions! Help ICBR Grow in Ten Quick Questions!
Help ICBR Grow in Ten Quick Questions!
We want to hear about your experience working with UF ICBR. What do you like? How can we improve? We want to hear from you. For your convenience, we kept our survey to ten quick questions. This is your opportunity to help us continue our quest to become the best resource on campus. Click Here to Participate in our Survey ...
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Help ICBR Grow in Ten Quick Questions!

Instruments

Staff

NameTitleTelephoneE-mailRoom
Sixue Chen, Ph.D.Proteomics & Mass Spectrometry Faculty Director(352) 273-8057schen@ufl.edu169A CGRC
Jin Koh, Ph.D.Proteomics & Mass Spectrometry Scientific Director(352) 273-8059jinkoh@ufl.edu168A CGRC
Tingting Wang, Ph.D.Proteomics & Mass Spectrometry Assistant Scientist(352) 273-8060wangt1@ufl.edu168 CGRC
Ran Zheng, M.S.Proteomics & Mass Spectrometry Core Research Specialist I(352) 273-8061ranzheng@ufl.edu169 CGRC
Ning Zhu, B.S.Proteomics & Mass Spectrometry Core Research Specialist I(352) 273-8067nzhu@ufl.edu169 CGRC
Fanchao Zhu, B.S.Proteomics & Mass Spectrometry Core Research Technician III(352) 273-8060fzhu9482@ufl.edu169 CGRC