Proteomics & Mass Spectrometry

OVERVIEW

ICBR Proteomics & Mass Spectrometry Facility has provided unprecedented tools for fast, accurate, high throughput biomolecular separation and characterization, which are indispensable towards understanding the biological and medical systems. Studying at the protein level allows researchers to investigate how proteins, their dynamics, modifications, and interactions affect cellular processes and how cellular processes and environment affect proteins. The mission of our facility is to provide excellent service and training in proteomics and mass spectrometry. Here we present our capabilities in proteomics, highlighting differential proteomics, which include two-dimensional difference gel electrophoresis (2D-DIGE), isobaric tags for relative and absolute quantitation (iTRAQ), tandem mass tags (TMT), iodoTMT, Stable isotope labeling with amino acids in cell culture (SILAC), quantification of proteins by label-free LC-MS/MS, and the analysis of protein post-translational modifications (PTM).

We have a suite of state-of-the-art mass spectrometers and software available for detailed characterization of proteins and peptides, including post-translational modification analysis and accurate molecular weight determination. To ensure success and maximize productivity, the Proteomics & Mass Spectrometry core offers education, consultation, data processing and reporting, and support of grant application.

CORE NEWS

A first: Scientists grow plants in soil from the Moon

A first: Scientists grow plants in soil from the Moon from UF/IFAS by Samantha Murray Scientists have grown plants in soil from the Moon, a first in human history and a milestone in lunar and space exploration. In a new paper published in the journal “Communications Biology,” University of Florida researchers showed that plants can successfully sprout and grow in lunar soil. Their study also investigated how plants respond biologically to the Moon’s soil, also known as lunar regolith, which is r...

SynGatorTron™ to speed medical research, alleviate privacy worries

from UF Health “Dr. Chatbot will see you now.” The next generation of super-smart computers, tablets and cell phones may come equipped with artificial intelligence-generated medical chatbots that can interact with patients using human language and medical knowledge. According to Yonghui Wu, Ph.D., director of natural language processing at the University of Florida Clinical and Translational Science Institute, the medical chatbot you interact with online will be able to use conversational langua...

UF Small Animal Hospital to offer free eye, heart screenings for service animals

UF Small Animal Hospital to offer free eye, heart screenings for service animals from UF Health The University of Florida’s Small Animal Hospital will offer free eye and heart screenings for service animals on May 20 as part of the American College of Veterinary Ophthalmologists-StokesRx National Service Animal Eye Exam event. Service animals typically include guide dogs, disability assistance dogs, detection dogs, police dogs, search and rescue dogs, and formally trained and certified therapy d...

Rhes protein key in spread of Huntington’s disease in the brain

Rhes protein key in spread of Huntington’s disease in the brain from UF Health By: Bill Levesque Pathogenic proteins help spread many neurodegenerative diseases. How they move between brain cells is often shrouded in mystery. But scientists at Scripps Research’s Florida campus have found that nanotube tunnels that act like roadways capable of transporting cargo between cells can transmit a toxic protein linked to Huntington’s disease from neuron to neuron in the live brains of mouse models. The ...

Soltis named 2021-2022 Teacher/Scholar of the Year

Soltis named 2021-2022 Teacher/Scholar of the Year from UF News by Blake Trauschke Pamela S. Soltis, a distinguished professor and curator at the Florida Museum of Natural History, has been selected as the 2021-2022 University of Florida Teacher/Scholar of the Year. UF’s most prestigious and oldest faculty award, the recognition offers an honorarium of $6,000 in addition to other acknowledgements. In selecting the winner, the Award Committee chooses a faculty member who demonstrates distinguish...

New COVID test can quickly detect variants

New COVID test can quickly detect variants from UF News University of Florida researchers have invented a test that can determine within 10-15 minutes whether patients test positive for COVID and, if so, which of the five known variants of concern they have. The research, published Saturday in The Lancet’s eBioMedicine, involves the use of a simple heating device and a cellphone. The team also used a new form of CRISPR — a means of finding and targeting a specific section of genetic materi...

UF | ICBR Invited to Speak at the 2022 Plant Biology Worldwide Summit

ICBR Director, Dr. Anna-Lisa Paul, and ICBR Proteomics & Mass Spectrometry Faculty Director, Dr. Sixue Chen, have been invited to speak at this year’s Plant Biology Worldwide Summit, hosted by the American Society of Plant Biologists. Dr. Paul’s will be on site to discuss Plants for Space, while Dr. Chen will address Applying Quantitative Proteomics in Plants. The annual plant biology meeting has been held for over eight decades and has been dedicated to the fostering of plant sc...

ICBR’s Dr. Yanping Zhang wins 2022 Superior Accomplishment Award

ICBR's Dr. Yanping Zhang wins 2022 Superior Accomplishment Award

Director of ICBR’s Gene Expression & Genotyping core, Dr. Yanping Zhang has been recognized as winner of the 2022 Superior Accomplishment Award! On February 16th, Dr. Zhang joined hundreds of other nominees for this prestigious recognition of her work. The Superior Accomplishment Award (SAA) program recognizes faculty and staff members who contribute outstanding and meritorious service, efficiency and/or economy, or to the quality of life provided to students and employees. Recognition...