Ettan DALTsix Electrophoresis
System function and description
In 2-D electrophoresis, proteins are separated according to isoelectric
point by isoelectric focusing, most reliably on Immobiline™ DryStrip
immobilized pH gradient (IPG) gel strips using the IPGphor™ or
Multiphor™ II IEF Systems. The second-dimension electrophoresis
separates the proteins on the basis of their molecular mass using sodium
dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The
Ettan™ DALTsix Electrophoresis System is designed to handle large
second-dimension gels in a simple, efficient, and reproducible manner.
The Ettan DALTsix Electrophoresis Unit accommodates up to six
25.5 × 20.5 cm slab gels, either 1 mm or 1.5 mm thick, in a common
tank under identical conditions. A sample, focused in an IPG strip, is
placed on the cathodic (upper) surface of a slab gel and sealed in place
with agarose. Up to six gel cassettes are inserted into the electrophoresis
unit and any unused slots are filled with blank cassette inserts. The Upper
Buffer Chamber/Buffer Seal (UBC) is pushed down over the cassettes,
holding the gel cassettes in slots flanked by a double rubber gasket.
Power is supplied to the unit by an external source such as an EPS 601
Power Supply. The unit is capable of handling 600 V, 400 mA, or 100 W.
A pump mounted under the lower buffer chamber circulates the buffer,
pumping it up into the chamber on the right-hand side, between the
cassettes, down the left side, and over the internal heat exchanger before
returning to the pump. The pump starts when it is plugged in. For temperature
control,the heat exchanger located in the bottom of the unitmust be connected
to a MultiTemp™ III or similar circulating water bath.
The Ettan DALTsix Gel Caster holds up to six 1-mm or 1.5-mm gel
cassettes, with separator sheets, for casting homogenous or gradient gels.
When desired, fewer gels can be cast at one time by using blank cassette
inserts to occupy any unneeded volume. The removable faceplate and
separator sheets simplify loading and unloading the casting unit. The
groove in the back of the caster provides the channel through which the
gel solution is poured into the caster unless gradients are being the cast
by displacement through the lower tubing fitting.