Useful Documents

A collection of SOPs, manuals, protocols and other helpful tools for users at UF | ICBR

USEFUL DOCUMENTS BY CORE

Monoclonal Antibody

Proteomics & Mass Spectrometry

PROTEOMICS PROTOCOLS 

Isobaric labeling Quantification (iTRAQ/TMT/iodoTMT): Isobaric-Labeling-Quantification

SILAC: SILAC

Protein Extraction: Protein-isolation-using-Phenol

PTM Sample Preparation and Analysis: PTM-Sample-Prep-and-Analysis

MRM/SRM and PRM: MRM-SRM-PRM

Protein N-terminal Labeling and Analysis: N-Terminal-Labeling-and-Analysis

Label-Free Shotgun Proteomics: Label-Free-Shotgun

2D-DIGE: 2D-DIGE

 

PROTEOMICS DELIVERABLES AND DATA VISUALIZATION 

Deliverables

  • -iTRAQ: Raw files and ProteinPilot 5.0 or Scaffold with Mascot 2.6 output (please choose one)
  • -TMT: Raw files and Proteome Discoverer 2.2 output
  • -Label-Free Proteomics: Raw files and Scaffold Q+S with Mascot 2.6 or Proteome Discoverer 2.2 output
  • -identification: Raw files and Scaffold Q+S with Mascot 2.6 (*.dat)

Scaffold Viewer

www.proteomesoftware.com/products/qplus-s/

ProteinPilot Viewer

sciex.com/products/software/proteinpilot-software

Proteome Discoverer Viewer

thermo.flexnetoperations.com/

Mascot Public Search

www.matrixscience.com/cgi/search_form.pl?FORMVER=2&SEARCH=MIS

Other Documents