Gene Expression & Genotyping

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GENE EXPRESSION & GENOTYPING:    Home  |  Staff & Contact  ServicesInstruments  |  Getting Started  |  Sample Requirements |  Core Resources

Email: ICBR-GeneExpression@ad.ufl.edu  | ICBR-Genotyping@ad.ufl.edu       Phone: (352) 273-8043 | (352) 273-8036

Overview

The Gene Expression & Genotyping core provides state-of-the-art technical services and consultation on Single Cell RNA-Seq, RNA-Seq, 16s metagenomics and Affymetrix gene expression arrays. High-throughput RNA-seq libraries, 16s libraries and sequencing capture libraries can be done by using the Agilent Bravo robot. QuantiGene RNA Assays measure up to 80 gene targets directly with degraded and cross-linked RNA in FFPE tissues and blood, with no RNA purification required. The BioRad QX200 AutoDG Droplet Digital PCR System provides absolute quantification of target DNA or RNA molecules with greater precision and sensitivity than qPCR, the sensitivty off ddPCR System can facilitate expanded analysis of single cells. The Genotyping services include fragment analysis using AB3730 (96 capillary technology), development of microsatellite libraries using Illumina sequence data, and genotyping using mouse tails or ear punches. These up-to-date gene expression and genotyping analysis methods allow scientists to be on the cutting edge of research.

ICBR Gene Expression & Genotyping Services

Don't see the service you are looking for? Visit ICBR NextGen DNA Sequencing for more options.

General Services

Library Construction Services

Single Cell Analysis

Genotyping Services

  • Project Consultation
  • Antibody Sequencing
  • Digital PCR services
  • Microarray Services
  • Sample Prep: RNA, DNA extraction
  • RNA DNA QC: Bioanalyzer/TapeStation, Qubit, PicoGreen
  • Sanger Sequencing Load only
  • CRISPR services
  • Bravo automation
  • QuantiGen assays (up to 80 targets): Gene Expression and CNV
  • 16s metagenomics library Automation
  • Illumina Directional RNA-Seq Library Preparation (Poly A)
  • Illumina Non-Directional RNA-Seq Library Preparation (Poly A)
  • Illumina RNA-Seq library Prep for low input, ribo-depletion or degraded RNA
  • Illumina Small RNA libray w/Barcode
  • RNA-Seq library automation
  • rRNAs Depletion
  • Agilent SureSelect Target Enrichment
  • IDT Exome Capture Library Construction with/without Pre-pooling
  • Takara Single Cell Library Construction
  • 16s library Automation Per Sample (batch w/48 samples, 96 samples)
  • Illumina RNA-Seq libraries (Poly A)
  • Illumina RNA-Seq library Prep for low input, ribo-depletion or degraded RNA
  • Illumina RNA-Seq library Prep (polyA with 140 samples and up, 500 samples and up)
  • Illumina Small RNA-Seq library w/Barcode
  • NEB Directional RNA-Seq Library Preparation
  • NEB Non-directional RNA-Seq Library preparation
  • NextGen Sequencing Library Quantification
  • RNA-Seq library automation per sample (24 samples, 48 samples, 96 samples)
  • rRNAs Depletion
  • RNA Extraction, RNA Clean-up
  • Agilent SureSelect Target Enrichment
  • IDT Exome Capture Library Construction with Pre-pooling, without Pre-pooling
  • 10X Genomics Chromium 3’ Single Cell RNA-Seq
  • 10X Genomics Chromium 5’ Single Cell RNA-Seq
  • 10X Genomics Chromium Single Cell CNV analysis
  • 10X Genomics Single Human/Mouse T cell V(D)J Library Prep (per sample)
  • 10X Genomics Chromium Single Cell Feature Barcode for CITE-Seq
  • 10X Genomics Chromium Single Cell Feature Barcode for CRISPR Screening
  • Fragment Analysis
  • STR Analysis
  • Cell Line Authentication
  • Copy Number Variance
  • SNP analysis by qPCR or digital PCR

Please note that our library construction and sequencing services do not include data analysis. Visit ICBR Bioinformatics to request Data Analysis services.

Core News

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Figure 1. Luminex bead architecture technology ICBR Proteomics & Mass Spectrometry (PM) Core is now offering access to the Luminex FLEXMAP 3D®   analytical platform for discovering and validation of protein biomarkers across a wide range of diseases. The FLEXMAP 3D® system combines differentially dyed fluorescent microsphere sets with an innovative instrument design to enable simultaneous measurement of  the concentration of a large number of different analytes in a rapid multiplexed as...
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Congratulations to UF’s Dr. Anna-Lisa Paul on receiving the NASA Exceptional Scientific Achievement Medal this past September! The prestigious award is reserved for individual efforts that are in line with NASA’s mission and have resulted in considerable scientific discovery or resulted in contributions of fundamental importance in their field or significantly improved the understanding of their field. Dr. Paul is the Interim Director at UF ICBR and Research Professor in the UF Horticultural...
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Dr. Anna-Lisa Paul receives NASA’s Exceptional Scientific Achievement Medal
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In a continued effort to provide accessible and specialized expertise to our users, UF ICBR is proud to announce our Core Directors’ Open Consultation Meetings. Each month we will be available for a two hour walk-in session to discuss the best approaches and strategies for using ICBR technologies and expertise to drive your research forward. Our first meeting will be on Monday, October 7th from 11:00am to 1:00pm in CGRC 189. No appointment is require nor do you have to be a current ICBR user t...
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Introducing Core Directors’ Open Consultation Meetings
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After much anticipation, the Illumina NovaSeq 6000 has arrived at UF ICBR. Users can expect access later this fall. The NovaSeq 6000 Sequencing System unleashes groundbreaking innovations that leverage our proven technology. Now you can get scalable throughput and flexibility for virtually any sequencing method, genome, and scale of project. The NovaSeq will be housed in our NextGen DNA Sequencing core. Stay tuned for more information and events this fall. For more information on the instrument...
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We want to hear about your experience working with UF ICBR. What do you like? How can we improve? We want to hear from you. For your convenience, we kept our survey to ten quick questions. This is your opportunity to help us continue our quest to become the best resource on campus. Click Here to Participate in our Survey ...
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