Gene Expression & Genotyping

CORE OVERVIEW

Welcome to UF ICBR Gene Expression and Genotyping Core (GE). Our up-to-date gene expression and genotyping analysis methods allow scientists to be on the cutting edge of research. Our high-quality data can help researchers for their grant applications and scientific paper publications.

The Gene Expression & Genotyping core provides state-of-the-art technical services and consultation on Single Cell RNA-Seq, 10x Visium Spatial Gene Expression, Covid-seq, RNA-Seq, 16s metagenomics and ThemoFisher Axiom Genotyping arrays using Beckman Biomek Liquid Handling Automation instrument. High-throughput RNA-seq libraries, 16s libraries and sequencing capture libraries can be done by using the SPT Labtech nanoliter pipetting instrument Mosquito, DrogenFly and Agilent Bravo robot. QuantiGene RNA Assays measure up to 80 gene targets directly with degraded and cross-linked RNA in FFPE tissues and blood, with no RNA purification required. The BioRad QX200 AutoDG Droplet Digital PCR System provides absolute quantification of target DNA or RNA molecules with greater precision and sensitivity than qPCR, the sensitivity off ddPCR System can facilitate expanded analysis of single cells.

To help researchers at every aspect, GE also provides a variety of basic molecular biology services such as DNA isolations, PCR reactions, and PCR purifications for sequencing. High quality of DNA and RNA are the key for reliable data; therefore, DNA and RNA quality assessment services are offered using an Agilent Bioanalyzer and TapeStation instrument at GE.

The walkup service for DNA RNA extraction with QIAcube is available at GE as well.

GE also offer CRISPR services, for more information, please contact us at GeneExpression@ad.ufl.edu

SERVICES OVERVIEW

General Services

Library Construction Services

Single Cell Analysis

Genotyping Services

Project Consultation
Antibody Sequencing
Digital PCR services
Sample Prep: RNA, DNA extraction
RNA DNA QC: Bioanalyzer/TapeStation, Qubit, PicoGreen
CRISPR services
Bravo automation
QuantiGen assays (up to 80 targets): Gene Expression and CNV

16s metagenomics library Automation
Illumina Directional RNA-Seq Library Preparation (Poly A)
Illumina Non-Directional RNA-Seq Library Preparation (Poly A)
Illumina RNA-Seq library Prep for low input, ribo-depletion or degraded RNA
Illumina Small RNA libray w/Barcode
RNA-Seq library automation
rRNAs Depletion
Agilent SureSelect Target Enrichment
IDT Exome Capture Library Construction with/without Pre-pooling
Takara Single Cell Library Construction
16s library Automation Per Sample (batch w/48 samples, 96 samples)
Illumina RNA-Seq libraries (Poly A)
Illumina RNA-Seq library Prep for low input, ribo-depletion or degraded RNA
Illumina RNA-Seq library Prep (polyA with 140 samples and up, 500 samples and up)
Illumina Small RNA-Seq library w/Barcode
NEB Directional RNA-Seq Library Preparation
NEB Non-directional RNA-Seq Library preparation
NextGen Sequencing Library Quantification
RNA-Seq library automation per sample (24 samples, 48 samples, 96 samples)
rRNAs Depletion
RNA Extraction, RNA Clean-up
Agilent SureSelect Target Enrichment
IDT Exome Capture Library Construction with Pre-pooling, without Pre-pooling

10X Genomics Chromium 3’ Single Cell RNA-Seq
10X Genomics Chromium 5’ Single Cell RNA-Seq
10X Genomics Chromium Single Cell CNV analysis
10X Genomics Single Human/Mouse T cell V(D)J Library Prep (per sample)
10X Genomics Chromium Single Cell Feature Barcode for CITE-Seq
10X Genomics Chromium Single Cell Feature Barcode for CRISPR Screening

SNP analysis by qPCR or digital PCR

Looking for data analysis services? Visit ICBR Bioinformatics for services.

Don’t see the service you are looking for? Visit ICBR NextGen DNA Sequencing for more options.

CORE NEWS

UF/IFAS study: Forget the queen, kill the brood to eliminate subterranean termite colonies

By Lourdes Mederos UF/IFAS DAVIE, Fla – Taking an extended look inside a subterranean termite colony is a rare and almost non-existent opportunity unless you raise one. But scientists like Thomas Chouvenc rear colonies from a king and queen, allowing them to produce thousands of eggs that grow to full maturity. In doing so, Chouvenc sheds a world of light on the species, the colony’s social behaviors, survival tactics and weaknesses. Thomas Chouvenc of UF IFAS Fort Lauderdale Research and Educ...

Hippos’ Constant Defecating Turns African Pools into Communal Guts

Lauren Barnett UF CLAS Hippopotamuses can eat nearly 100 pounds of food daily — and, as a result, they fill the pools where they spend much of their lives with huge amounts of poop. All that excrement, new research has found, turns the pools into extensions of the hippos’ guts, as bacteria and other microbes expelled into the water survive and are shared among the congregating animals. This “meta-gut,” as the researchers termed it, could have major impacts on the hippos’ ecosystems. The findings...

Hot Tumors: The Turbo Engine That Immunotherapy Needed

by Maja Aganovic UF Innovate Immunotherapy is a hot topic of conversation when it comes to cancer treatment. Pediatric oncologist Dr. Elias Sayour and his team have found a way to make a hot tumor, their way to make immunotherapy, an alternative to chemotherapy, more effective. “I spend the bulk of my time doing research, 80 percent of my time trying to develop new therapies for childhood cancer,” said Sayour, an associate professor of neurosurgery and pediatrics and principal investigator of th...

UF Study: Consumers like Shelved Tomato Juice More than a Refrigerated Product

by Brad Buck UF/IFAS Tempted by the tangy taste of tomato juice? Consumers may prefer a product sold from grocery store shelves over a minimally pasteurized refrigerated product, but only by a small margin, new University of Florida research shows. Researchers at the UF Institute of Food and Agricultural Sciences made four types of tomato juice, combining six UF/IFAS tomato varieties. In this study, scientists wanted to know how heat treatments impact flavor compounds in tomato juices. Generally...

Even at the Cellular Level, Ancestry Matters

by Emily Cardinali UF News Diseases impact different populations with varying prevalence, which is reflected in health disparities that are seen for illnesses like lupus, cancer, and cardiovascular disease. To create effective medical treatments, researchers need representative population samples. Even though disease impacts everybody, medical research samples are often not representative of diverse populations. In a new article published by Nature, UF professors Erika Moore, Josephine Allen and...

Two Common Compounds Show Effectiveness Against COVID-19 Virus in Early Testing

by Doug Bennett UF Health A pair of over-the-counter compounds has been found in preliminary tests to inhibit the virus that causes COVID-19, University of Florida Health researchers have found. The combination includes diphenhydramine, an antihistamine used for allergy symptoms. When paired with lactoferrin, a protein found in cow and human milk, the compounds were found to hinder the SARS-CoV-2 virus during tests in monkey cells and human lung cells. The findings by David A. Ostrov, Ph.D., an ...

Stomata Molecular Switches and the Future of Agriculture with Dr. Sixue Chen

By Rosie Kereston UF Genetics Institute Dr. Sixue Chen, professor in the Department of Biology and faculty director of the UF | ICBR Proteomics and Mass Spectrometry core, has dedicated his research to improving the future of agriculture and global food security. “I have always wanted to do something useful for other people. That keeps me going. Every day, I ask myself how I can make a positive difference in the lives of as many people as possible. That’s why I love my job- it is a perfect...

Underbite regained: Species feared extinct is the only frog with true teeth on its lower jaw

By Jerald Pinson Florida Museum In a new study, biologists laid to rest a century-old debate by confirming that a single species of frog, out of the more than 7,000 living today, has true teeth on its lower jaw. The culprit, a large marsupial frog named Gastrotheca guentheri, has puzzled scientists since its discovery in 1882 for possessing what appeared to be a complete set of jagged, daggerlike teeth on the top and bottom of its mouth. Scientists have since waffled over the exact nature of the...