Gene Expression & Genotyping

ICBR-Genotyping@ad.ufl.edu   ///   ICBR-GeneExpression@ad.ufl.edu   ///   (352) 273-8043   ///   RRID:SCR_019145
Single Cell RNA-Seq, CRISPR Services, Digital PCR and More

CORE OVERVIEW

Welcome to UF ICBR Gene Expression and Genotyping Core (GE). Our up-to-date gene expression and genotyping analysis methods allow scientists to be on the cutting edge of research. Our high-quality data can help researchers for their grant applications and scientific paper publications.

The Gene Expression & Genotyping core provides state-of-the-art technical services and consultation on Single Cell RNA-Seq, 10x Visium Spatial Gene Expression, Covid-seq, RNA-Seq, 16s metagenomics and ThemoFisher Axiom Genotyping arrays using Beckman Biomek Liquid Handling Automation instrument. High-throughput RNA-seq libraries, 16s libraries and sequencing capture libraries can be done by using the SPT Labtech nanoliter pipetting instrument Mosquito, DragonFly and Agilent Bravo robot. QuantiGene RNA Assays measure up to 80 gene targets directly with degraded and cross-linked RNA in FFPE tissues and blood, with no RNA purification required. The BioRad QX200 AutoDG Droplet Digital PCR System provides absolute quantification of target DNA or RNA molecules with greater precision and sensitivity than qPCR, the sensitivity off ddPCR System can facilitate expanded analysis of single cells.

To help researchers at every aspect, GE also provides a variety of basic molecular biology services such as DNA isolations, PCR reactions, and PCR purifications for sequencing. High quality of DNA and RNA are the key for reliable data; therefore, DNA and RNA quality assessment services are offered using an Agilent Bioanalyzer and TapeStation instrument at GE.

The walkup service for DNA RNA extraction with QIAcube is available at GE as well, for more information, please contact us at GeneExpression@ad.ufl.edu

SERVICES OVERVIEW

General Services

Library Construction Services

Single Cell Analysis

Genotyping Services

  • Project Consultation
  • Digital PCR services
  • RNA DNA QC: Bioanalyzer/TapeStation, Qubit, PicoGreen
  • CRISPR services
  • Automated NextGen Library Preparation
  • QuantiGen assays (up to 80 targets): Gene Expression and CNV
  • 16s metagenomics library Automation
  • Illumina Directional RNA-Seq Library Preparation (Poly A)
  • Illumina Non-Directional RNA-Seq Library Preparation (Poly A)
  • Illumina RNA-Seq library Prep for low input, ribo-depletion or degraded RNA
  • Illumina Small RNA libray w/Barcode
  • RNA-Seq library automation
  • rRNAs Depletion
  • Agilent SureSelect Target Enrichment
  • IDT Exome Capture Library Construction with/without Pre-pooling
  • Takara Single Cell Library Construction
  • 16s library Automation Per Sample (batch w/48 samples, 96 samples)
  • Illumina RNA-Seq libraries (Poly A)
  • Illumina RNA-Seq library Prep for low input, ribo-depletion or degraded RNA
  • Illumina RNA-Seq library Prep (polyA with 140 samples and up, 500 samples and up)
  • Illumina Small RNA-Seq library w/Barcode
  • NEB Directional RNA-Seq Library Preparation
  • NEB Non-directional RNA-Seq Library preparation
  • NextGen Sequencing Library Quantification
  • RNA-Seq library automation per sample (24 samples, 48 samples, 96 samples)
  • rRNAs Depletion
  • RNA Extraction, RNA Clean-up
  • Agilent SureSelect Target Enrichment
  • IDT Exome Capture Library Construction with Pre-pooling, without Pre-pooling
  • 10X Genomics Chromium 3’ Single Cell RNA-Seq
  • 10X Genomics Chromium 5’ Single Cell RNA-Seq
  • 10X Genomics Chromium Single Cell CNV analysis
  • 10X Genomics Single Human/Mouse T cell V(D)J Library Prep (per sample)
  • 10X Genomics Chromium Single Cell Feature Barcode for CITE-Seq
  • 10X Genomics Chromium Single Cell Feature Barcode for CRISPR Screening
  • SNP analysis by qPCR or digital PCR
Looking for data analysis services? Visit ICBR Bioinformatics for services.
Don’t see the service you are looking for? Visit ICBR NextGen DNA Sequencing for more options.

CORE NEWS

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ICBR Closed Friday for a Strategic Retreat ICBR will be closed from 8am-5pm on Friday (7/18) for a special All Staff Strategic Retreat. We will not be accepting packages or samples on Friday. Our normal business hours will resume on Monday. Please refer to our staff directory for additional details. ...
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ICBR Closed Friday (7/18) for Team Building
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ICBR Closed Friday for the 4th of July ICBR will be closed this Friday (7/4) in observation of the Fourth of July   We will not be accepting packages or samples on Friday. Our normal business hours will resume on Monday (7/7). Enjoy the the long weekend!   Please refer to our staff directory for additional details. ...
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ICBR Closed Friday for the 4th of July
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Environmental DNA – It’s All Up in the Air Steven J. Madore, PhD (ICBR Associate Director for Science) (Note: this article has been summarized from UF News “Environmental DNA floating in the air tracks wildlife, viruses — even drugs” June 3, 2025 by Eric Hamilton) Scientists can now use the power of genetic information to identify biodiversity by sampling environmental DNA or eDNA. “The level of information that’s available in environmental DNA is such that we’re only starting to consider what t...
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ICBR Closed Thursday for Juneteenth ICBR will be closed this Thursday (6/19) in observation of Juneteenth   We will not be accepting packages or samples on Thursday. Our normal business hours will resume on Friday (6/20).   Please refer to our staff directory for additional details. ...
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ICBR Closed Thursday for Juneteenth
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UF Investigators Identify Mechanism of Drug Resistance in Non-Small Cell Lung Cancer Steven J Madore, PhD (ICBR Associate Director for Science) Summarized from J. Clin. Inv. 2025;135(6); e185149 Non-small cell lung cancer (NSCLC) is a leading cause of cancer-related deaths worldwide. Despite a combination of surgery and chemotherapy, survival rate remains low due to cancer cell metastasis and drug resistance.  Mutations in the oncogene KRAS are frequently found in human cancers, including up to ...
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New Instruments in the ICBR NGS Shared Resource Will Lower DNA Sequencing Costs for UF Investigators Steven Madore, PhD, ICBR Associate Director for Science The mission of the ICBR Next Generation DNA Sequencing shared resource (NGS-SR) is to provide cost-effective access to cutting edge DNA sequencing technologies for the UF research community. The NGS-SR offers library preparation and sequencing instrumentation for both short (300-500 base pairs fragments) and long read (5,000 to 25,000 base p...
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New Instruments in the ICBR NGS Shared Resource Lowers DNA Sequencing Costs for UF Investigators
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CORE INSTRUMENTS

Gene Expression & Genotyping