Gene Expression & Genotyping

ICBR-Genotyping@ad.ufl.edu   ///   ICBR-GeneExpression@ad.ufl.edu   ///   (352) 273-8043   ///   RRID:SCR_019145
Single Cell RNA-Seq, CRISPR Services, Digital PCR and More

CORE OVERVIEW

Welcome to UF ICBR Gene Expression and Genotyping Core (GE). Our up-to-date gene expression and genotyping analysis methods allow scientists to be on the cutting edge of research. Our high-quality data can help researchers for their grant applications and scientific paper publications.

The Gene Expression & Genotyping core provides state-of-the-art technical services and consultation on Single Cell RNA-Seq, 10x Visium Spatial Gene Expression, Covid-seq, RNA-Seq, 16s metagenomics and ThemoFisher Axiom Genotyping arrays using Beckman Biomek Liquid Handling Automation instrument. High-throughput RNA-seq libraries, 16s libraries and sequencing capture libraries can be done by using the SPT Labtech nanoliter pipetting instrument Mosquito, DragonFly and Agilent Bravo robot. QuantiGene RNA Assays measure up to 80 gene targets directly with degraded and cross-linked RNA in FFPE tissues and blood, with no RNA purification required. The BioRad QX200 AutoDG Droplet Digital PCR System provides absolute quantification of target DNA or RNA molecules with greater precision and sensitivity than qPCR, the sensitivity off ddPCR System can facilitate expanded analysis of single cells.

To help researchers at every aspect, GE also provides a variety of basic molecular biology services such as DNA isolations, PCR reactions, and PCR purifications for sequencing. High quality of DNA and RNA are the key for reliable data; therefore, DNA and RNA quality assessment services are offered using an Agilent Bioanalyzer and TapeStation instrument at GE.

The walkup service for DNA RNA extraction with QIAcube is available at GE as well, for more information, please contact us at GeneExpression@ad.ufl.edu

SERVICES OVERVIEW

General Services

Library Construction Services

Single Cell Analysis

Genotyping Services

  • Project Consultation
  • Antibody Sequencing
  • Digital PCR services
  • Sample Prep: RNA, DNA extraction
  • RNA DNA QC: Bioanalyzer/TapeStation, Qubit, PicoGreen
  • CRISPR services
  • Bravo automation
  • QuantiGen assays (up to 80 targets): Gene Expression and CNV
  • 16s metagenomics library Automation
  • Illumina Directional RNA-Seq Library Preparation (Poly A)
  • Illumina Non-Directional RNA-Seq Library Preparation (Poly A)
  • Illumina RNA-Seq library Prep for low input, ribo-depletion or degraded RNA
  • Illumina Small RNA libray w/Barcode
  • RNA-Seq library automation
  • rRNAs Depletion
  • Agilent SureSelect Target Enrichment
  • IDT Exome Capture Library Construction with/without Pre-pooling
  • Takara Single Cell Library Construction
  • 16s library Automation Per Sample (batch w/48 samples, 96 samples)
  • Illumina RNA-Seq libraries (Poly A)
  • Illumina RNA-Seq library Prep for low input, ribo-depletion or degraded RNA
  • Illumina RNA-Seq library Prep (polyA with 140 samples and up, 500 samples and up)
  • Illumina Small RNA-Seq library w/Barcode
  • NEB Directional RNA-Seq Library Preparation
  • NEB Non-directional RNA-Seq Library preparation
  • NextGen Sequencing Library Quantification
  • RNA-Seq library automation per sample (24 samples, 48 samples, 96 samples)
  • rRNAs Depletion
  • RNA Extraction, RNA Clean-up
  • Agilent SureSelect Target Enrichment
  • IDT Exome Capture Library Construction with Pre-pooling, without Pre-pooling
  • 10X Genomics Chromium 3’ Single Cell RNA-Seq
  • 10X Genomics Chromium 5’ Single Cell RNA-Seq
  • 10X Genomics Chromium Single Cell CNV analysis
  • 10X Genomics Single Human/Mouse T cell V(D)J Library Prep (per sample)
  • 10X Genomics Chromium Single Cell Feature Barcode for CITE-Seq
  • 10X Genomics Chromium Single Cell Feature Barcode for CRISPR Screening
  • SNP analysis by qPCR or digital PCR
Looking for data analysis services? Visit ICBR Bioinformatics for services.
Don’t see the service you are looking for? Visit ICBR NextGen DNA Sequencing for more options.

CORE NEWS

Fueling Discoveries Together: Acknowledge UF ICBR in Your Publications Fueling Discoveries Together: Acknowledge UF ICBR in Your Publications
Fueling Discoveries Together: Acknowledge UF ICBR in Your Publications
Fueling Discoveries Together: Acknowledge UF ICBR in Your Publications In our pursuit of advancing scientific discovery, the UF Interdisciplinary Center for Biotechnology Research (UF ICBR) is committed to facilitating seamless acknowledgments for your invaluable contributions. We introduce Research Resource Identifiers (RRIDs), ensuring a straightforward and precise referencing system for our resources. Simply copy and paste the relevant core RRID, conveniently located here. Proper acknowledgm...
Read More
Fueling Discoveries Together: Acknowledge UF ICBR in Your Publications
Nadia Brown Joins UF ICBR as Assistant Director of Operations Nadia Brown Joins UF ICBR as Assistant Director of Operations
Nadia Brown Joins UF ICBR as Assistant Director of Operations
Nadia Brown Joins UF ICBR as Assistant Director of Operations The University of Florida Interdisciplinary Center for Biotechnology Research (ICBR) welcomes Nadia Brown as the Assistant Director of Operations. Nadia brings a wealth of experience and expertise to the position with a Bachelor of Science in Business Administration, majoring in accounting and finance from Kansas State University, and a Master of Science in Family, Youth, and Community Sciences with a financial planning concentration ...
Read More
Nadia Brown Joins UF ICBR as Assistant Director of Operations
ICBR Electron Microscopy Core Acquires Talos L120C ICBR Electron Microscopy Core Acquires Talos L120C
ICBR Electron Microscopy Core Acquires Talos L120C
ICBR Electron Microscopy Core Acquires Talos L120C The University of Florida Interdisciplinary Center for Biotechnology Research is excited to announce the latest addition to its Electron Microscopy core: the Thermo Scientific Talos L120C G2, a 120 kV Transmission Electron Microscope. This state-of-the-art instrument brings a new level of flexibility and imaging capabilities for researchers. One of the key advantages of the Talos is its user-friendly interface, which allows our staff to easily...
Read More
ICBR Electron Microscopy Core Acquires Talos L120C
Feeding the Community: UF ICBR’s 6th Annual Backpack Food Drive Competition Feeding the Community: UF ICBR’s 6th Annual Backpack Food Drive Competition
Feeding the Community: UF ICBR's 6th Annual Backpack Food Drive Competition
Feeding the Community: UF ICBR’s 6th Annual Backpack Food Drive Competition The 6th Annual Backpack Food Drive Competition was recently held by UF ICBR, with tremendous success. During this competition, all of our cores came together to make a significant impact by donating a total of 9,540 servings of food. “Food insecurity is always a concern and especially in recent years as food prices have increased,” shared Erica Boyd, Human Resources Generalist III at ICBR, who organized...
Read More
Feeding the Community: UF ICBR’s 6th Annual Backpack Food Drive Competition
ICBR Enhances DNA Sequencing Capabilities with the Illumina NovaSeq X Plus ICBR Enhances DNA Sequencing Capabilities with the Illumina NovaSeq X Plus
ICBR Enhances DNA Sequencing Capabilities with the Illumina NovaSeq X Plus
ICBR Enhances DNA Sequencing Capabilities with the Illumina NovaSeq X Plus The ICBR Next Generation DNA Sequencing Core is thrilled to announce the introduction of the Illumina NovaSeq X Plus DNA sequencing platform! This revolutionary system brings a multitude of advantages, including enhanced throughput, reduced run times, and uncompromised sequencing data quality, surpassing the capabilities of its predecessor, the NovaSeq6000. Perhaps one of the most remarkable features of the NovaSeq X Plus...
Read More
ICBR Enhances DNA Sequencing Capabilities with the Illumina NovaSeq X Plus
Signaling Molecules Help Plants Fight Microbes – ICBR Scientists Contribute to Groundbreaking Research Signaling Molecules Help Plants Fight Microbes – ICBR Scientists Contribute to Groundbreaking Research
Signaling Molecules Help Plants Fight Microbes - ICBR Scientists Contribute to Groundbreaking Research
Signaling Molecules Help Plants Fight Microbes – ICBR Scientists Contribute to Groundbreaking Research Scientists from the University of Florida Interdisciplinary Center for Biotechnology Research (ICBR) have made a significant contribution to a study published in the journal Nature Communications that advances our understanding of plant immune response. The research study was led by Dr. Zhonglin Mou in the UF Molecular and Cell Sciences Department and Dr. Shweta Chhajed in the UF Departme...
Read More
Signaling Molecules Help Plants Fight Microbes – ICBR Scientists Contribute to Groundbreaking Research
High School Students Dive into Biotechnology at the UF Interdisciplinary Center for Biotechnology Research High School Students Dive into Biotechnology at the UF Interdisciplinary Center for Biotechnology Research
High School Students Dive into Biotechnology at the UF Interdisciplinary Center for Biotechnology Research
High School Students Dive into Biotech at the UF Interdisciplinary Center for Biotechnology Research Last week, high school students from Mount Dora Christian Academy visited the Interdisciplinary Center for Biotechnology Research (ICBR). During their visit, they explored the expansive world of biotechnology and they learned about the ICBR’s pivotal role in advancing discoveries in the life sciences at UF. “Hosting visits of young aspiring scientists like this allows students with an interes...
Read More
High School Students Dive into Biotechnology at the UF Interdisciplinary Center for Biotechnology Research
ICBR Gene Expression & Genotyping Acquires LeviCell ICBR Gene Expression & Genotyping Acquires LeviCell
ICBR Gene Expression & Genotyping Acquires LeviCell
ICBR Gene Expression & Genotyping Acquires LeviCell ICBR GE has recently acquired the LeviCell system, a cutting-edge technology that is now available for walk-up service. This innovative system uses magnetic levitation to separate live cells from dead cells and debris, making it a reliable, stress-free, and label-free solution for sorting cell populations. Here’s how it works: First, the cell suspension is mixed with a reagent and loaded into the LeviCell cartridge. Then, the cartridg...
Read More
ICBR Gene Expression & Genotyping Acquires LeviCell

CORE INSTRUMENTS

Gene Expression & Genotyping