Gene Expression & Genotyping

ICBR-Genotyping@ad.ufl.edu   ///   ICBR-GeneExpression@ad.ufl.edu   ///   (352) 273-8043   ///   RRID:SCR_019145
Single Cell RNA-Seq, CRISPR Services, Digital PCR and More

CORE OVERVIEW

Welcome to UF ICBR Gene Expression and Genotyping Core (GE). Our up-to-date gene expression and genotyping analysis methods allow scientists to be on the cutting edge of research. Our high-quality data can help researchers for their grant applications and scientific paper publications.

The Gene Expression & Genotyping core provides state-of-the-art technical services and consultation on Single Cell RNA-Seq, 10x Visium Spatial Gene Expression, Covid-seq, RNA-Seq, 16s metagenomics and ThemoFisher Axiom Genotyping arrays using Beckman Biomek Liquid Handling Automation instrument. High-throughput RNA-seq libraries, 16s libraries and sequencing capture libraries can be done by using the SPT Labtech nanoliter pipetting instrument Mosquito, DrogenFly and Agilent Bravo robot. QuantiGene RNA Assays measure up to 80 gene targets directly with degraded and cross-linked RNA in FFPE tissues and blood, with no RNA purification required. The BioRad QX200 AutoDG Droplet Digital PCR System provides absolute quantification of target DNA or RNA molecules with greater precision and sensitivity than qPCR, the sensitivity off ddPCR System can facilitate expanded analysis of single cells.

To help researchers at every aspect, GE also provides a variety of basic molecular biology services such as DNA isolations, PCR reactions, and PCR purifications for sequencing. High quality of DNA and RNA are the key for reliable data; therefore, DNA and RNA quality assessment services are offered using an Agilent Bioanalyzer and TapeStation instrument at GE.

The walkup service for DNA RNA extraction with QIAcube is available at GE as well. 

GE also offer CRISPR services, for more information, please contact us at GeneExpression@ad.ufl.edu

SERVICES OVERVIEW

General Services

Library Construction Services

Single Cell Analysis

Genotyping Services

  • Project Consultation
  • Antibody Sequencing
  • Digital PCR services
  • Sample Prep: RNA, DNA extraction
  • RNA DNA QC: Bioanalyzer/TapeStation, Qubit, PicoGreen
  • CRISPR services
  • Bravo automation
  • QuantiGen assays (up to 80 targets): Gene Expression and CNV
  • 16s metagenomics library Automation
  • Illumina Directional RNA-Seq Library Preparation (Poly A)
  • Illumina Non-Directional RNA-Seq Library Preparation (Poly A)
  • Illumina RNA-Seq library Prep for low input, ribo-depletion or degraded RNA
  • Illumina Small RNA libray w/Barcode
  • RNA-Seq library automation
  • rRNAs Depletion
  • Agilent SureSelect Target Enrichment
  • IDT Exome Capture Library Construction with/without Pre-pooling
  • Takara Single Cell Library Construction
  • 16s library Automation Per Sample (batch w/48 samples, 96 samples)
  • Illumina RNA-Seq libraries (Poly A)
  • Illumina RNA-Seq library Prep for low input, ribo-depletion or degraded RNA
  • Illumina RNA-Seq library Prep (polyA with 140 samples and up, 500 samples and up)
  • Illumina Small RNA-Seq library w/Barcode
  • NEB Directional RNA-Seq Library Preparation
  • NEB Non-directional RNA-Seq Library preparation
  • NextGen Sequencing Library Quantification
  • RNA-Seq library automation per sample (24 samples, 48 samples, 96 samples)
  • rRNAs Depletion
  • RNA Extraction, RNA Clean-up
  • Agilent SureSelect Target Enrichment
  • IDT Exome Capture Library Construction with Pre-pooling, without Pre-pooling
  • 10X Genomics Chromium 3’ Single Cell RNA-Seq
  • 10X Genomics Chromium 5’ Single Cell RNA-Seq
  • 10X Genomics Chromium Single Cell CNV analysis
  • 10X Genomics Single Human/Mouse T cell V(D)J Library Prep (per sample)
  • 10X Genomics Chromium Single Cell Feature Barcode for CITE-Seq
  • 10X Genomics Chromium Single Cell Feature Barcode for CRISPR Screening
  • SNP analysis by qPCR or digital PCR
Looking for data analysis services? Visit ICBR Bioinformatics for services.
Don’t see the service you are looking for? Visit ICBR NextGen DNA Sequencing for more options.

CORE NEWS

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Dear ICBR colleagues and customers, The COVID19 Delta Variant is causing many of the concerns we all had in the early stages of the pandemic, and as a consequence, there is a recognition that we as a community can help mitigate the spread by also returning to some of the precautions we adopted last year. Because ICBR and UF Health share work spaces, we will adopt the same masking policy that is currently being implemented within Shands/UF Health. This practice means that everyone within ICBR pub...
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By Steven Madore, Ph.D. In recent years, public health emergencies caused by epidemics have led to the use of genome sequencing to identify and characterize viral pathogens. Rapid acquisition of high quality viral genomic sequences is critical for understanding viral pathogen origin, transmission and epidemiological spread. Ultimately deciphering the molecular characteristics of viruses accelerates the development of diagnostic assays and vaccine development and drug design and is important in u...
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Courtesy of UF/IFAS Written by Brad Buck  Feral pigs cost the agriculture industry at least $1.5 billion in damage, disease and control costs around the United States annually, running rampant on large swaths of grazing lands. The swine root in soil and eat most everything in sight. Farmers and ranchers will benefit from research by University of Florida scientists who are using artificial intelligence to gather data on feral hog reproduction and movement. As they roam grazing land, feral swine ...
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Courtesy of the Florida Museum of Natural History  Written by Natalie van Hoose  In a genetic surprise, ancient DNA shows the closest family members of an extinct bird known as the Haitian cave-rail are not in the Americas, but Africa and the South Pacific, uncovering an unexpected link between Caribbean bird life and the Old World. Like many animals unique to the Caribbean, cave-rails became extinct soon after people settled the islands. The last of three known West Indian species of cave-rails...
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CORE INSTRUMENTS

Gene Expression & Genotyping