Gene Expression & Genotyping

Home

GENE EXPRESSION & GENOTYPING: Home | Staff & Contact | Services | Instruments | Getting Started | Sample Requirements | Core Resources

Email: ICBR-GeneExpression@ad.ufl.edu | ICBR-Genotyping@ad.ufl.edu Phone: (352) 273-8043 | (352) 273-8036

Overview

The Gene Expression & Genotyping core provides state-of-the-art technical services and consultation on Single Cell RNA-Seq, RNA-Seq, 16s metagenomics and Affymetrix gene expression arrays. High-throughput RNA-seq libraries, 16s libraries and sequencing capture libraries can be done by using the Agilent Bravo robot. QuantiGene RNA Assays measure up to 80 gene targets directly with degraded and cross-linked RNA in FFPE tissues and blood, with no RNA purification required. The BioRad QX200 AutoDG Droplet Digital PCR System provides absolute quantification of target DNA or RNA molecules with greater precision and sensitivity than qPCR, the sensitivity off ddPCR System can facilitate expanded analysis of single cells. The Genotyping services include fragment analysis using AB3730 (96 capillary technology), development of microsatellite libraries using Illumina sequence data, and genotyping using mouse tails or ear punches. These up-to-date gene expression and genotyping analysis methods allow scientists to be on the cutting edge of research.

ICBR Gene Expression & Genotyping Services

Don't see the service you are looking for? Visit ICBR NextGen DNA Sequencing for more options.

General Services

Library Construction Services

Single Cell Analysis

Genotyping Services

Project Consultation
Antibody Sequencing
Digital PCR services
Microarray Services
Sample Prep: RNA, DNA extraction
RNA DNA QC: Bioanalyzer/TapeStation, Qubit, PicoGreen
Sanger Sequencing Load only
CRISPR services
Bravo automation
QuantiGen assays (up to 80 targets): Gene Expression and CNV

16s metagenomics library Automation
Illumina Directional RNA-Seq Library Preparation (Poly A)
Illumina Non-Directional RNA-Seq Library Preparation (Poly A)
Illumina RNA-Seq library Prep for low input, ribo-depletion or degraded RNA
Illumina Small RNA libray w/Barcode
RNA-Seq library automation
rRNAs Depletion
Agilent SureSelect Target Enrichment
IDT Exome Capture Library Construction with/without Pre-pooling
Takara Single Cell Library Construction
16s library Automation Per Sample (batch w/48 samples, 96 samples)
Illumina RNA-Seq libraries (Poly A)
Illumina RNA-Seq library Prep for low input, ribo-depletion or degraded RNA
Illumina RNA-Seq library Prep (polyA with 140 samples and up, 500 samples and up)
Illumina Small RNA-Seq library w/Barcode
NEB Directional RNA-Seq Library Preparation
NEB Non-directional RNA-Seq Library preparation
NextGen Sequencing Library Quantification
RNA-Seq library automation per sample (24 samples, 48 samples, 96 samples)
rRNAs Depletion
RNA Extraction, RNA Clean-up
Agilent SureSelect Target Enrichment
IDT Exome Capture Library Construction with Pre-pooling, without Pre-pooling

10X Genomics Chromium 3’ Single Cell RNA-Seq
10X Genomics Chromium 5’ Single Cell RNA-Seq
10X Genomics Chromium Single Cell CNV analysis
10X Genomics Single Human/Mouse T cell V(D)J Library Prep (per sample)
10X Genomics Chromium Single Cell Feature Barcode for CITE-Seq
10X Genomics Chromium Single Cell Feature Barcode for CRISPR Screening

Fragment Analysis
STR Analysis
Cell Line Authentication
Copy Number Variance
SNP analysis by qPCR or digital PCR

Please note that our library construction and sequencing services do not include data analysis. Visit ICBR Bioinformatics to request Data Analysis services.

Core News

UPDATE: ICBR Continues to Provide Support to UF Community

With the progression of recent events, there are a lot of questions about how it affects ICBR users. Please see below, important changes that could impact your experience at ICBR. These changes will be in place for the foreseeable future. Please refer back to this page for timely updates. As always, check the UF dedicated site for campus-wide information and initiatives. LATEST NEWS ICBR is now open, Monday through Friday, to support research on the University of Florida campus. Please be...

UF | ICBR: Shut Down for Academics for Black Lives

UF | ICBR will be closed on the afternoon of Friday, June 19th, starting at noon and ending at 5pm, to allow employees time to reflect on our personal actions and continue to educate ourselves about racism. These efforts are in conjunction with those of the President of the University of Florida and Academics for Black Lives, an initiative intended for academic faculty and staff to examine the toll of racial trauma on Black people, combat racism and hate, and seek both accountability and colle...

Dr. Anna-Lisa Paul Appointed Director of UF ICBR

The University of Florida Interdisciplinary Center for Biotechnology Research recently announced the appointment of its new director, Anna-Lisa Paul, Ph.D. Dr. Paul assumed the position effective March 20, 2020. For the past year, Dr. Paul has served as ICBR’s interim director. During that time, Dr. Paul spearheaded the center’s acquisition of state-of-the-art equipment and furthered ICBR’s collaborative relationship with UF Research departments. “The University of Florida Health Cancer Center h...

ICBR Contributes to First Genomic Sequence of COVID-19 in New Orleans, LA

While clinicians are working on the front lines to treat patients with COVID-19, researchers are working behind the scenes, investigating the viral life cycle and advancing new developments in therapeutics and vaccines. Critical to this research effort is determining the genetic code the virus uses for infection and propagation. UF | ICBR is doing its part to provide essential services to drive this research further; delivering support not only locally, but on a national scale. In a recent colla...

UF Research Community Joins Hands to Donate Gloves, Masks and Other PPE

The same personal protective equipment (PPE) that is used in research labs is also used in clinical settings. Currently, this equipment is incredibly valuable as health workers are on the front lines treating COVID-19. In keeping with the UF spirit of collaboration and innovation, researchers from across the University of Florida donated thousands of PPE to support local medical workers.The solicitation of the donation drive was orchestrated by UF|Research. The donated supplies, including gloves...

ICBR Cyberinfrastructure: Zoom into Security

Recent information has shown that Zoom is not able to encrypt ALL methods of entry into a meeting, i.e. by calling in on a phone. Although Zoom is doing its best to encrypt and secure our video meetings and chats, we should ensure all university data is securely handled and that we are acting exactly as we would in an office, (i.e. attire, language, etc.) in these meetings. There have been countless recent reports, both at UF and outside of the university, of “Zoombombing” (when an uninvi...

BI associate scientist contributes to two publications

Bioinformatics associate scientist Fahong Yu, Ph.D. provided research assistance in two recent publications (2019). Oral epithelial cells (OEC) that line the oral cavity are the first line of defense against pathogenic organisms including viruses that infect the body through the oral route. The role of the OEC in triggering the immune response to viral infection is poorly understood. ICBR Bioinformatics expert Dr. Fahong Yu performed data analysis and was a key collaborator in a study performed ...

BI assistant scientist develops a tool for stratifying cancer patients

Bioinformatics assistant scientist Tongjun Gu Ph.D. has developed an advanced deep learning method to stratify cancer patients into clinically relevant subgroups and further identify the potential biomarkers between the subgroups. This will help with the development of personalized treatments and prognosis. The method can integrate multi-platform datasets like gene expression, miRNA expression, methylation, copy number alteration, and protein expression. It can also do transfer learning between ...