Gene Expression & Genotyping

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GENE EXPRESSION & GENOTYPING: Home | Staff & Contact | Services | Instruments | Getting Started | Sample Requirements | Core Resources

Email: ICBR-GeneExpression@ad.ufl.edu | ICBR-Genotyping@ad.ufl.edu Phone: (352) 273-8043 | (352) 273-8036

Overview

The Gene Expression & Genotyping core provides state-of-the-art technical services and consultation on Single Cell RNA-Seq, RNA-Seq, 16s metagenomics and Affymetrix gene expression arrays. High-throughput RNA-seq libraries, 16s libraries and sequencing capture libraries can be done by using the Agilent Bravo robot. QuantiGene RNA Assays measure up to 80 gene targets directly with degraded and cross-linked RNA in FFPE tissues and blood, with no RNA purification required. The BioRad QX200 AutoDG Droplet Digital PCR System provides absolute quantification of target DNA or RNA molecules with greater precision and sensitivity than qPCR, the sensitivty off ddPCR System can facilitate expanded analysis of single cells. The Genotyping services include fragment analysis using AB3730 (96 capillary technology), development of microsatellite libraries using Illumina sequence data, and genotyping using mouse tails or ear punches. These up-to-date gene expression and genotyping analysis methods allow scientists to be on the cutting edge of research.

ICBR Gene Expression & Genotyping Services

Don't see the service you are looking for? Visit ICBR NextGen DNA Sequencing for more options.

General Services

Library Construction Services

Single Cell Analysis

Genotyping Services

Project Consultation
Antibody Sequencing
Digital PCR services
Microarray Services
Sample Prep: RNA, DNA extraction
RNA DNA QC: Bioanalyzer/TapeStation, Qubit, PicoGreen
Sanger Sequencing Load only
CRISPR services
Bravo automation
QuantiGen assays (up to 80 targets): Gene Expression and CNV

16s metagenomics library Automation
Illumina Directional RNA-Seq Library Preparation (Poly A)
Illumina Non-Directional RNA-Seq Library Preparation (Poly A)
Illumina RNA-Seq library Prep for low input, ribo-depletion or degraded RNA
Illumina Small RNA libray w/Barcode
RNA-Seq library automation
rRNAs Depletion
Agilent SureSelect Target Enrichment
IDT Exome Capture Library Construction with/without Pre-pooling
Takara Single Cell Library Construction
16s library Automation Per Sample (batch w/48 samples, 96 samples)
Illumina RNA-Seq libraries (Poly A)
Illumina RNA-Seq library Prep for low input, ribo-depletion or degraded RNA
Illumina RNA-Seq library Prep (polyA with 140 samples and up, 500 samples and up)
Illumina Small RNA-Seq library w/Barcode
NEB Directional RNA-Seq Library Preparation
NEB Non-directional RNA-Seq Library preparation
NextGen Sequencing Library Quantification
RNA-Seq library automation per sample (24 samples, 48 samples, 96 samples)
rRNAs Depletion
RNA Extraction, RNA Clean-up
Agilent SureSelect Target Enrichment
IDT Exome Capture Library Construction with Pre-pooling, without Pre-pooling

10X Genomics Chromium 3’ Single Cell RNA-Seq
10X Genomics Chromium 5’ Single Cell RNA-Seq
10X Genomics Chromium Single Cell CNV analysis
10X Genomics Single Human/Mouse T cell V(D)J Library Prep (per sample)
10X Genomics Chromium Single Cell Feature Barcode for CITE-Seq
10X Genomics Chromium Single Cell Feature Barcode for CRISPR Screening

Fragment Analysis
STR Analysis
Cell Line Authentication
Copy Number Variance
SNP analysis by qPCR or digital PCR

Please note that our library construction and sequencing services do not include data analysis. Visit ICBR Bioinformatics to request Data Analysis services.

Core News

ICBR Bioinformatics Contributes to Landmark UFHCC Publication

The ICBR Bioinformatics Core contributed to a high-profile paper from the cancer research laboratory of UFHCC Director Dr. Jonathan Licht, recently published in Cancer Discovery. The paper, entitled “A Mutation in Histone H2B Represents a New Class of Oncogenic Driver”, describes how mutations in core histones disrupt nucleosome stability, leading to disregulation of gene expression and acting as a novel oncogenic mechanism. As part of this study the investigators used a technique ca...

Help ICBR Grow in Ten Quick Questions!

We want to hear about your experience working with UF ICBR. What do you like? How can we improve? We want to hear from you. For your convenience, we kept our survey to ten quick questions. This is your opportunity to help us continue our quest to become the best resource on campus. Click Here to Participate in our Survey ...

Instrument Spotlight: Protein Simple Wes

Wes™ lets you separate and analyze proteins by size from 2-440 kDa. He also gets you down to pg-level sensitivity with just 3 µL of starting material. Got a lot of samples and no time? No problem. Wes runs up to 25 samples in 3 hours flat and gives you quantitative, size-based data including total protein. Don’t wait to discover, get going today with Wes! Our Simple Western systems offer the right throughput for your needs. Whether you are looking to process up to 24 samples per run or ...

Instrument Spotlight: PacBio Sequel

GAIN A COMPREHENSIVE VIEW OF GENOMES, TRANSCRIPTOMES, AND EPIGENOMES – Create high-quality whole genome de novo assemblies of eukaryotic organisms – Survey large population cohorts and resolve structural variants – Read full-length transcripts to characterize isoform diversity – Target all variant types across relevant genomic regions – Detect epigenetic modifications simultaneously Get Started: Contact ICBR NextGen DNA Sequencing Click here to see SEQUEL Services &...

ICBR Closed: September 2nd and September 3rd

ICBR will be closed on Monday, September 2nd in observation of Labor Day, and on Tuesday, September 3rd due to Hurricane Dorian. There is a chance we will be closed on Wednesday; please confirm our availability via phone or email. ...

Single Cell Genomics on the 10X Genomics Chromium System

The ICBR and UFHCC have partnered in the acquisition of new cutting-edge technology with the capability of analyzing gene structure and gene activity at the single cell level. The Chromium instrument developed by California-based 10X Genomics inc. provides two unique key technologies to the UF research community. In one application, the instrument uses synthetic gel beads to partition thousands of separate enzymatic reactions necessary for downstream DNA sequencing. In another application, short...

CRISPR at UF ICBR

In collaboration with the UFHCC, ICBR is pleased to announce several new CRISPR genome editing services now available to the UF research community. The CRISPR/Cas9 system allows researchers to easily introduce deletions, insertions and edited DNA sequences into mammalian cell genomes. It can be used to introduce very specific surgical edits to any gene and can in fact introduce foreign genes into specific regions of the animal cell genome. At the University of Florida, CRISPR is used within re...

Is Your Monoclonal Antibody Authentic? Confirming Hybridoma Cell Line Identity by IgC cDNA Sequencing

Widespread reports of irreproducibility in health sciences research has prompted the NIH to update new grant application guidelines to include a brief description of methods that will be used to ensure identity and validity of key biological and/or chemical resources used in the proposed study. In this regard there is a significant effort now underway using DNA “fingerprinting” as a way to use microsatellite repeat lengths as a powerful tool for human cell line authentication. This method ha...