Gene Expression & Genotyping
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GENE EXPRESSION & GENOTYPING: Home | Staff & Contact | Services | Instruments | Getting Started | Sample Requirements | Core Resources
Email: ICBR-GeneExpression@ad.ufl.edu | ICBR-Genotyping@ad.ufl.edu Phone: (352) 273-8043 | (352) 273-8036
Overview
The Gene Expression & Genotyping core provides state-of-the-art technical services and consultation on Single Cell RNA-Seq, RNA-Seq, 16s metagenomics and Affymetrix gene expression arrays. High-throughput RNA-seq libraries, 16s libraries and sequencing capture libraries can be done by using the Agilent Bravo robot. QuantiGene RNA Assays measure up to 80 gene targets directly with degraded and cross-linked RNA in FFPE tissues and blood, with no RNA purification required. The BioRad QX200 AutoDG Droplet Digital PCR System provides absolute quantification of target DNA or RNA molecules with greater precision and sensitivity than qPCR, the sensitivity off ddPCR System can facilitate expanded analysis of single cells. The Genotyping services include fragment analysis using AB3730 (96 capillary technology), development of microsatellite libraries using Illumina sequence data, and genotyping using mouse tails or ear punches. These up-to-date gene expression and genotyping analysis methods allow scientists to be on the cutting edge of research.
ICBR Gene Expression & Genotyping Services
Don't see the service you are looking for? Visit ICBR NextGen DNA Sequencing for more options.
General Services
Library Construction Services
Single Cell Analysis
Genotyping Services
- Project Consultation
- Antibody Sequencing
- Digital PCR services
- Sample Prep: RNA, DNA extraction
- RNA DNA QC: Bioanalyzer/TapeStation, Qubit, PicoGreen
- Sanger Sequencing Load only
- CRISPR services
- Bravo automation
- QuantiGen assays (up to 80 targets): Gene Expression and CNV
- 16s metagenomics library Automation
- Illumina Directional RNA-Seq Library Preparation (Poly A)
- Illumina Non-Directional RNA-Seq Library Preparation (Poly A)
- Illumina RNA-Seq library Prep for low input, ribo-depletion or degraded RNA
- Illumina Small RNA libray w/Barcode
- RNA-Seq library automation
- rRNAs Depletion
- Agilent SureSelect Target Enrichment
- IDT Exome Capture Library Construction with/without Pre-pooling
- Takara Single Cell Library Construction
- 16s library Automation Per Sample (batch w/48 samples, 96 samples)
- Illumina RNA-Seq libraries (Poly A)
- Illumina RNA-Seq library Prep for low input, ribo-depletion or degraded RNA
- Illumina RNA-Seq library Prep (polyA with 140 samples and up, 500 samples and up)
- Illumina Small RNA-Seq library w/Barcode
- NEB Directional RNA-Seq Library Preparation
- NEB Non-directional RNA-Seq Library preparation
- NextGen Sequencing Library Quantification
- RNA-Seq library automation per sample (24 samples, 48 samples, 96 samples)
- rRNAs Depletion
- RNA Extraction, RNA Clean-up
- Agilent SureSelect Target Enrichment
- IDT Exome Capture Library Construction with Pre-pooling, without Pre-pooling
- 10X Genomics Chromium 3’ Single Cell RNA-Seq
- 10X Genomics Chromium 5’ Single Cell RNA-Seq
- 10X Genomics Chromium Single Cell CNV analysis
- 10X Genomics Single Human/Mouse T cell V(D)J Library Prep (per sample)
- 10X Genomics Chromium Single Cell Feature Barcode for CITE-Seq
- 10X Genomics Chromium Single Cell Feature Barcode for CRISPR Screening
- Fragment Analysis
- STR Analysis
- Cell Line Authentication
- Copy Number Variance
- SNP analysis by qPCR or digital PCR
Please note that our library construction and sequencing services do not include data analysis. Visit ICBR Bioinformatics to request Data Analysis services.
Core News
Important FY2021 Fiscal Dates
Important FY2021 Fiscal Dates
As the fiscal year comes to an end, we want to make sure you don’t forget the significant dates on the horizon. Please see below:
Please note: Due to the Fiscal Year End, the last invoices to process for FY2021 will be created on Monday, June 14th.Due to Fiscal Year End, ICBR’s upcoming billing schedule will be altered as follows:
6/14/2021 – Last invoices generated in fiscal year 2021
6/21/2021 at noon – Deadline to submit disputes for FY2021 payment.
7/5/2021 – Invoices wil... Read More
UF Researcher Dr. Marco Salemi Utilizes ICBR Genomics Expertise to Understand COVID19 Epidemiology
UF Researcher Dr. Marco Salemi Utilizes ICBR Genomics Expertise to Understand COVID19 Epidemiology
By Steven Madore, Ph.D.
In recent years, public health emergencies caused by epidemics have led to the use of genome sequencing to identify and characterize viral pathogens. Rapid acquisition of high quality viral genomic sequences is critical for understanding viral pathogen origin, transmission and epidemiological spread. Ultimately deciphering the molecular characteristics of viruses accelerates the development of diagnostic assays and vaccine development and drug design and is important in u... Read More
UF researchers use AI datasets to track feral pigs, minimize disease risk
UF researchers use AI datasets to track feral pigs, minimize disease risk
Courtesy of UF/IFAS
Written by Brad Buck
Feral pigs cost the agriculture industry at least $1.5 billion in damage, disease and control costs around the United States annually, running rampant on large swaths of grazing lands. The swine root in soil and eat most everything in sight.
Farmers and ranchers will benefit from research by University of Florida scientists who are using artificial intelligence to gather data on feral hog reproduction and movement.
As they roam grazing land, feral swine ... Read More
Extinct Caribbean bird’s closest relatives hail from Africa, South Pacific
Extinct Caribbean bird’s closest relatives hail from Africa, South Pacific
Courtesy of the Florida Museum of Natural History
Written by Natalie van Hoose
In a genetic surprise, ancient DNA shows the closest family members of an extinct bird known as the Haitian cave-rail are not in the Americas, but Africa and the South Pacific, uncovering an unexpected link between Caribbean bird life and the Old World.
Like many animals unique to the Caribbean, cave-rails became extinct soon after people settled the islands. The last of three known West Indian species of cave-rails... Read More
UF | ICBR Scientists Contribute to Clinical Trial Testing Novel Treatment for Canine Atopic Dermatitis
UF | ICBR Scientists Contribute to Clinical Trial Testing Novel Treatment for Canine Atopic Dermatitis
Atopic dermatitis, or AD, is a common chronic inflammatory skin disease affecting dogs, causing defects of the skin barrier and modifications of the normal cutaneous microflora. While multiple treatment options are available, there is a growing interest in the use of alternative natural ingredient products. In human AD reports show the potential benefits of topical applications of heat-killed beneficial bacteria, with some studies demonstrating the efficacy of rapidly killed beneficial la... Read More
UF researchers to use AI to predict how hurricanes spread invasive plants
UF researchers to use AI to predict how hurricanes spread invasive plants
Courtesy of UF/IFAS
Written by Brad Buck
Scientists project hurricane intensity and frequency will increase with climate change. That leads researchers to want to better predict how storms will disperse and establish nonnative plant invaders.
Knowing where invasive plants spread will always be useful. But it may become especially handy soon as the National Hurricane Center is considering moving the official start of the Atlantic hurricane season two weeks earlier, starting it May 15, instead of ... Read More
World-Renowned UF Health Biochemistry Researcher Passes Away
World-Renowned UF Health Biochemistry Researcher Passes Away
Courtesy of College of Medicine News
March 3, 2021 — University of Florida Health biochemistry professor Mavis Agbandje-McKenna, Ph.D., whose world-renowned work on the detailed structure of viruses led to advances in gene therapy treatments for different diseases, died Wednesday at her home near Gainesville of the neurodegenerative disease amyotrophic lateral sclerosis. She was 57.
Agbandje-McKenna’s work with the small, infectious particles had a worldwide impact, allowing other scientists to ... Read More
UF Health Virologist Talks about Variants and the Future of the Coronavirus
UF Health Virologist Talks about Variants and the Future of the Coronavirus
Courtesy of UF Health
Written by Bill Levesque
The novel coronavirus is doing what viruses do — in fact, what all life does. It’s mutating and creating new variants. This is evolution 101.
Much of the discussion about the coronavirus in recent weeks has been about the potential impact of these variants on COVID-19 vaccines now being administered worldwide. Just what does the future hold?
University of Florida Health virologist John Lednicky, Ph.D., a professor in the in the UF College of Public ... Read More
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