Gene Expression & Genotyping

ICBR-Genotyping@ad.ufl.edu   ///   ICBR-GeneExpression@ad.ufl.edu   ///   (352) 273-8043   ///   RRID:SCR_019145
Single Cell RNA-Seq, CRISPR Services, Digital PCR and More

CORE OVERVIEW

Welcome to UF ICBR Gene Expression and Genotyping Core (GE). Our up-to-date gene expression and genotyping analysis methods allow scientists to be on the cutting edge of research. Our high-quality data can help researchers for their grant applications and scientific paper publications.

The Gene Expression & Genotyping core provides state-of-the-art technical services and consultation on Single Cell RNA-Seq, 10x Visium Spatial Gene Expression, Covid-seq, RNA-Seq, 16s metagenomics and ThemoFisher Axiom Genotyping arrays using Beckman Biomek Liquid Handling Automation instrument. High-throughput RNA-seq libraries, 16s libraries and sequencing capture libraries can be done by using the SPT Labtech nanoliter pipetting instrument Mosquito, DrogenFly and Agilent Bravo robot. QuantiGene RNA Assays measure up to 80 gene targets directly with degraded and cross-linked RNA in FFPE tissues and blood, with no RNA purification required. The BioRad QX200 AutoDG Droplet Digital PCR System provides absolute quantification of target DNA or RNA molecules with greater precision and sensitivity than qPCR, the sensitivity off ddPCR System can facilitate expanded analysis of single cells.

To help researchers at every aspect, GE also provides a variety of basic molecular biology services such as DNA isolations, PCR reactions, and PCR purifications for sequencing. High quality of DNA and RNA are the key for reliable data; therefore, DNA and RNA quality assessment services are offered using an Agilent Bioanalyzer and TapeStation instrument at GE.

The walkup service for DNA RNA extraction with QIAcube is available at GE as well. 

GE also offer CRISPR services, for more information, please contact us at GeneExpression@ad.ufl.edu

SERVICES OVERVIEW

General Services

Library Construction Services

Single Cell Analysis

Genotyping Services

  • Project Consultation
  • Antibody Sequencing
  • Digital PCR services
  • Sample Prep: RNA, DNA extraction
  • RNA DNA QC: Bioanalyzer/TapeStation, Qubit, PicoGreen
  • CRISPR services
  • Bravo automation
  • QuantiGen assays (up to 80 targets): Gene Expression and CNV
  • 16s metagenomics library Automation
  • Illumina Directional RNA-Seq Library Preparation (Poly A)
  • Illumina Non-Directional RNA-Seq Library Preparation (Poly A)
  • Illumina RNA-Seq library Prep for low input, ribo-depletion or degraded RNA
  • Illumina Small RNA libray w/Barcode
  • RNA-Seq library automation
  • rRNAs Depletion
  • Agilent SureSelect Target Enrichment
  • IDT Exome Capture Library Construction with/without Pre-pooling
  • Takara Single Cell Library Construction
  • 16s library Automation Per Sample (batch w/48 samples, 96 samples)
  • Illumina RNA-Seq libraries (Poly A)
  • Illumina RNA-Seq library Prep for low input, ribo-depletion or degraded RNA
  • Illumina RNA-Seq library Prep (polyA with 140 samples and up, 500 samples and up)
  • Illumina Small RNA-Seq library w/Barcode
  • NEB Directional RNA-Seq Library Preparation
  • NEB Non-directional RNA-Seq Library preparation
  • NextGen Sequencing Library Quantification
  • RNA-Seq library automation per sample (24 samples, 48 samples, 96 samples)
  • rRNAs Depletion
  • RNA Extraction, RNA Clean-up
  • Agilent SureSelect Target Enrichment
  • IDT Exome Capture Library Construction with Pre-pooling, without Pre-pooling
  • 10X Genomics Chromium 3’ Single Cell RNA-Seq
  • 10X Genomics Chromium 5’ Single Cell RNA-Seq
  • 10X Genomics Chromium Single Cell CNV analysis
  • 10X Genomics Single Human/Mouse T cell V(D)J Library Prep (per sample)
  • 10X Genomics Chromium Single Cell Feature Barcode for CITE-Seq
  • 10X Genomics Chromium Single Cell Feature Barcode for CRISPR Screening
  • SNP analysis by qPCR or digital PCR
Looking for data analysis services? Visit ICBR Bioinformatics for services.
Don’t see the service you are looking for? Visit ICBR NextGen DNA Sequencing for more options.

CORE NEWS

UF/IFAS study: Forget the queen, kill the brood to eliminate subterranean termite colonies UF/IFAS study: Forget the queen, kill the brood to eliminate subterranean termite colonies
UF/IFAS study: Forget the queen, kill the brood to eliminate subterranean termite colonies
By Lourdes Mederos UF/IFAS DAVIE, Fla –  Taking an extended look inside a subterranean termite colony is a rare and almost non-existent opportunity unless you raise one. But scientists like Thomas Chouvenc rear colonies from a king and queen, allowing them to produce thousands of eggs that grow to full maturity. In doing so, Chouvenc sheds a world of light on the species, the colony’s social behaviors, survival tactics and weaknesses. Thomas Chouvenc of UF IFAS Fort Lauderdale Research and Educ...
Read More
UF/IFAS study: Forget the queen, kill the brood to eliminate subterranean termite colonies
Hippos’ Constant Defecating Turns African Pools into Communal Guts Hippos’ Constant Defecating Turns African Pools into Communal Guts
Hippos’ Constant Defecating Turns African Pools into Communal Guts
Lauren Barnett UF CLAS Hippopotamuses can eat nearly 100 pounds of food daily — and, as a result, they fill the pools where they spend much of their lives with huge amounts of poop. All that excrement, new research has found, turns the pools into extensions of the hippos’ guts, as bacteria and other microbes expelled into the water survive and are shared among the congregating animals. This “meta-gut,” as the researchers termed it, could have major impacts on the hippos’ ecosystems. The findings...
Read More
Hippos’ Constant Defecating Turns African Pools into Communal Guts
Hot Tumors: The Turbo Engine That Immunotherapy Needed Hot Tumors: The Turbo Engine That Immunotherapy Needed
Hot Tumors: The Turbo Engine That Immunotherapy Needed
by Maja Aganovic UF Innovate Immunotherapy is a hot topic of conversation when it comes to cancer treatment. Pediatric oncologist Dr. Elias Sayour and his team have found a way to make a hot tumor, their way to make immunotherapy, an alternative to chemotherapy, more effective. “I spend the bulk of my time doing research, 80 percent of my time trying to develop new therapies for childhood cancer,” said Sayour, an associate professor of neurosurgery and pediatrics and principal investigator of th...
Read More
Hot Tumors: The Turbo Engine That Immunotherapy Needed
UF Study: Consumers like Shelved Tomato Juice More than a Refrigerated Product UF Study: Consumers like Shelved Tomato Juice More than a Refrigerated Product
UF Study: Consumers like Shelved Tomato Juice More than a Refrigerated Product
by Brad Buck UF/IFAS Tempted by the tangy taste of tomato juice? Consumers may prefer a product sold from grocery store shelves over a minimally pasteurized refrigerated product, but only by a small margin, new University of Florida research shows. Researchers at the UF Institute of Food and Agricultural Sciences made four types of tomato juice, combining six UF/IFAS tomato varieties. In this study, scientists wanted to know how heat treatments impact flavor compounds in tomato juices. Generally...
Read More
UF Study: Consumers like Shelved Tomato Juice More than a Refrigerated Product
Even at the Cellular Level, Ancestry Matters Even at the Cellular Level, Ancestry Matters
Even at the Cellular Level, Ancestry Matters
by Emily Cardinali UF News Diseases impact different populations with varying prevalence, which is reflected in health disparities that are seen for illnesses like lupus, cancer, and cardiovascular disease. To create effective medical treatments, researchers need representative population samples. Even though disease impacts everybody, medical research samples are often not representative of diverse populations. In a new article published by Nature, UF professors Erika Moore, Josephine Allen and...
Read More
Even at the Cellular Level, Ancestry Matters
Two Common Compounds Show Effectiveness Against COVID-19 Virus in Early Testing Two Common Compounds Show Effectiveness Against COVID-19 Virus in Early Testing
Two Common Compounds Show Effectiveness Against COVID-19 Virus in Early Testing
by Doug Bennett UF Health A pair of over-the-counter compounds has been found in preliminary tests to inhibit the virus that causes COVID-19, University of Florida Health researchers have found. The combination includes diphenhydramine, an antihistamine used for allergy symptoms. When paired with lactoferrin, a protein found in cow and human milk, the compounds were found to hinder the SARS-CoV-2 virus during tests in monkey cells and human lung cells. The findings by David A. Ostrov, Ph.D., an ...
Read More
Two Common Compounds Show Effectiveness Against COVID-19 Virus in Early Testing
Stomata Molecular Switches and the Future of Agriculture with Dr. Sixue Chen Stomata Molecular Switches and the Future of Agriculture with Dr. Sixue Chen
Stomata Molecular Switches and the Future of Agriculture with Dr. Sixue Chen
By Rosie Kereston UF Genetics Institute Dr. Sixue Chen, professor in the Department of Biology and faculty director of the UF | ICBR Proteomics and Mass Spectrometry core, has dedicated his research to improving the future of agriculture and global food security. “I have always wanted to do something useful for other people. That keeps me going. Every day, I ask myself how I can make a positive difference in the lives of as many people as possible. That’s why I love my job- it is a perfect...
Read More
Stomata Molecular Switches and the Future of Agriculture with Dr. Sixue Chen
Underbite regained: Species feared extinct is the only frog with true teeth on its lower jaw Underbite regained: Species feared extinct is the only frog with true teeth on its lower jaw
Underbite regained: Species feared extinct is the only frog with true teeth on its lower jaw
By Jerald Pinson Florida Museum In a new study, biologists laid to rest a century-old debate by confirming that a single species of frog, out of the more than 7,000 living today, has true teeth on its lower jaw. The culprit, a large marsupial frog named Gastrotheca guentheri, has puzzled scientists since its discovery in 1882 for possessing what appeared to be a complete set of jagged, daggerlike teeth on the top and bottom of its mouth. Scientists have since waffled over the exact nature of the...
Read More
Underbite regained: Species feared extinct is the only frog with true teeth on its lower jaw

CORE INSTRUMENTS

Gene Expression & Genotyping