Gene Expression & Genotyping

ICBR-Genotyping@ad.ufl.edu   ///   ICBR-GeneExpression@ad.ufl.edu   ///   (352) 273-8043   ///   RRID:SCR_019145
Single Cell RNA-Seq, CRISPR Services, Digital PCR and More

CORE OVERVIEW

Welcome to UF ICBR Gene Expression and Genotyping Core (GE). Our up-to-date gene expression and genotyping analysis methods allow scientists to be on the cutting edge of research. Our high-quality data can help researchers for their grant applications and scientific paper publications.

The Gene Expression & Genotyping core provides state-of-the-art technical services and consultation on Single Cell RNA-Seq, 10x Visium Spatial Gene Expression, Covid-seq, RNA-Seq, 16s metagenomics and ThemoFisher Axiom Genotyping arrays using Beckman Biomek Liquid Handling Automation instrument. High-throughput RNA-seq libraries, 16s libraries and sequencing capture libraries can be done by using the SPT Labtech nanoliter pipetting instrument Mosquito, DragonFly and Agilent Bravo robot. QuantiGene RNA Assays measure up to 80 gene targets directly with degraded and cross-linked RNA in FFPE tissues and blood, with no RNA purification required. The BioRad QX200 AutoDG Droplet Digital PCR System provides absolute quantification of target DNA or RNA molecules with greater precision and sensitivity than qPCR, the sensitivity off ddPCR System can facilitate expanded analysis of single cells.

To help researchers at every aspect, GE also provides a variety of basic molecular biology services such as DNA isolations, PCR reactions, and PCR purifications for sequencing. High quality of DNA and RNA are the key for reliable data; therefore, DNA and RNA quality assessment services are offered using an Agilent Bioanalyzer and TapeStation instrument at GE.

The walkup service for DNA RNA extraction with QIAcube is available at GE as well. 

GE also offer CRISPR services, for more information, please contact us at GeneExpression@ad.ufl.edu

SERVICES OVERVIEW

General Services

Library Construction Services

Single Cell Analysis

Genotyping Services

  • Project Consultation
  • Antibody Sequencing
  • Digital PCR services
  • Sample Prep: RNA, DNA extraction
  • RNA DNA QC: Bioanalyzer/TapeStation, Qubit, PicoGreen
  • CRISPR services
  • Bravo automation
  • QuantiGen assays (up to 80 targets): Gene Expression and CNV
  • 16s metagenomics library Automation
  • Illumina Directional RNA-Seq Library Preparation (Poly A)
  • Illumina Non-Directional RNA-Seq Library Preparation (Poly A)
  • Illumina RNA-Seq library Prep for low input, ribo-depletion or degraded RNA
  • Illumina Small RNA libray w/Barcode
  • RNA-Seq library automation
  • rRNAs Depletion
  • Agilent SureSelect Target Enrichment
  • IDT Exome Capture Library Construction with/without Pre-pooling
  • Takara Single Cell Library Construction
  • 16s library Automation Per Sample (batch w/48 samples, 96 samples)
  • Illumina RNA-Seq libraries (Poly A)
  • Illumina RNA-Seq library Prep for low input, ribo-depletion or degraded RNA
  • Illumina RNA-Seq library Prep (polyA with 140 samples and up, 500 samples and up)
  • Illumina Small RNA-Seq library w/Barcode
  • NEB Directional RNA-Seq Library Preparation
  • NEB Non-directional RNA-Seq Library preparation
  • NextGen Sequencing Library Quantification
  • RNA-Seq library automation per sample (24 samples, 48 samples, 96 samples)
  • rRNAs Depletion
  • RNA Extraction, RNA Clean-up
  • Agilent SureSelect Target Enrichment
  • IDT Exome Capture Library Construction with Pre-pooling, without Pre-pooling
  • 10X Genomics Chromium 3’ Single Cell RNA-Seq
  • 10X Genomics Chromium 5’ Single Cell RNA-Seq
  • 10X Genomics Chromium Single Cell CNV analysis
  • 10X Genomics Single Human/Mouse T cell V(D)J Library Prep (per sample)
  • 10X Genomics Chromium Single Cell Feature Barcode for CITE-Seq
  • 10X Genomics Chromium Single Cell Feature Barcode for CRISPR Screening
  • SNP analysis by qPCR or digital PCR
Looking for data analysis services? Visit ICBR Bioinformatics for services.
Don’t see the service you are looking for? Visit ICBR NextGen DNA Sequencing for more options.

CORE NEWS

Dr. David Moraga: 30 Years of Advancing Scientific Excellence at ICBR Dr. David Moraga: 30 Years of Advancing Scientific Excellence at ICBR
Dr. David Moraga: 30 Years of Advancing Scientific Excellence at ICBR
Dr. David Moraga: 30 Years of Advancing Scientific Excellence at ICBR David Moraga, PhD, the NextGen Sequencing director at the ICBR, is retiring after 30 years of service. Since 1993, Dr. Moraga has played a vital role in advancing the center’s mission of providing cutting-edge scientific technology and research support services to the global scientific community. “Dr. David Moraga’s name has been almost synonymous with DNA sequencing for decades at ICBR and in the UF biotech communi...
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Dr. David Moraga: 30 Years of Advancing Scientific Excellence at ICBR
Labor Day Notice: ICBR Closed on September 4th Labor Day Notice: ICBR Closed on September 4th
Labor Day Notice: ICBR Closed on September 4th
Labor Day Notice: ICBR Closed on September 4th Please note that the ICBR will be closed on September 4th in observance of Labor Day. Kindly be aware that we will not be able to accept any packages or samples during this time. We will resume regular operations on the following business day. Please refer to our staff directory for additional details. ...
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Labor Day Notice: ICBR Closed on September 4th
ICBR Closed Wednesday, August 30th for Tropical Storm Idalia ICBR Closed Wednesday, August 30th for Tropical Storm Idalia
ICBR Closed Wednesday, August 30th for Tropical Storm Idalia
ICBR Closed Wednesday, August 30th for Tropical Storm Idalia Due to Tropical Storm Idalia, the ICBR will close beginning at noon on (8/29) through Wednesday (8/30). We will not be accepting packages or samples during this time. The university expects to make an announcement on Wednesday regarding the resumption of normal campus operations. Please visit www.ufl.edu for updates and information on storm preparation.   Please refer to our staff directory for additional details. ...
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ICBR Closed Wednesday, August 30th for Tropical Storm Idalia
ICBR Gene Expression & Genotyping Acquires LeviCell ICBR Gene Expression & Genotyping Acquires LeviCell
ICBR Gene Expression & Genotyping Acquires LeviCell
ICBR Gene Expression & Genotyping Acquires LeviCell ICBR GE has recently acquired the LeviCell system, a cutting-edge technology that is now available for walk-up service. This innovative system uses magnetic levitation to separate live cells from dead cells and debris, making it a reliable, stress-free, and label-free solution for sorting cell populations. Here’s how it works: First, the cell suspension is mixed with a reagent and loaded into the LeviCell cartridge. Then, the cartridg...
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ICBR Gene Expression & Genotyping Acquires LeviCell
Dr. Alberto Riva at ISMB/ECCB2023: Advancing Bioinformatics Cores and Inspiring Collaboration Dr. Alberto Riva at ISMB/ECCB2023: Advancing Bioinformatics Cores and Inspiring Collaboration
Dr. Alberto Riva at ISMB/ECCB2023: Advancing Bioinformatics Cores and Inspiring Collaboration
Audience at the BioInfo Core Session at ISMB 2023 Dr. Alberto Riva at ISMB/ECCB2023: Advancing Bioinformatics Cores and Inspiring Collaboration Dr. Alberto Riva, the Scientific Director for the ICBR Bioinformatics core, recently attended the highly acclaimed Intelligent Systems for Molecular Biology (ISMB) conference, renowned as the largest and most prestigious gathering in the field of computational biology and bioinformatics.  This year’s conference, held in Lyon, France in partnership w...
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Dr. Alberto Riva at ISMB/ECCB2023: Advancing Bioinformatics Cores and Inspiring Collaboration
Dr. Diansy Zincke Named Scientific Director of ICBR NextGeneration DNA Sequencing Dr. Diansy Zincke Named Scientific Director of ICBR NextGeneration DNA Sequencing
Dr. Diansy Zincke Named Scientific Director of ICBR NextGeneration DNA Sequencing
Dr. Diansy Zincke Named Scientific Director of ICBR NextGeneration DNA Sequencing The University of Florida Interdisciplinary Center for Biotechnology Research (ICBR) recently announced the appointment of Dr. Diansy Zincke as the new Scientific Director of the Next Generation DNA Sequencing Core (NS). Dr. Zincke, who has been working with the ICBR since April 2021 as a Research Facility Specialist and Lab Manager, brings with her more than seven years of experience in the field of biology. Dr. Z...
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Dr. Diansy Zincke Named Scientific Director of ICBR NextGeneration DNA Sequencing
ICBR’s Dr. Anna-Lisa Paul Honored as Lifetime Fellow by the AAAS ICBR’s Dr. Anna-Lisa Paul Honored as Lifetime Fellow by the AAAS
ICBR's Dr. Anna-Lisa Paul Honored as Lifetime Fellow by the AAAS
The American Association for the Advancement of Science honors 19 UF faculty as Lifetime Fellows Dr. Anna-Lisa Paul, alongside 18 other UF faculty, has been honored as a Lifetime Fellow of AAAS. Dr. Paul was honored for “distinguished contributions to the understanding of life’s ability to move beyond planet Earth, as well as extraordinary service to the space biology science community”. Her research focuses on how plants respond to environmental change, with emphasis on spaceflight...
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ICBR’s Dr. Anna-Lisa Paul Honored as Lifetime Fellow by the AAAS
Surviving Space: Extreme Plant Adaptation Surviving Space: Extreme Plant Adaptation
Surviving Space: Extreme Plant Adaptation
Which plants have the best chances for survival in space? That’s the question being addressed by Principal Investigators Anna-Lisa Paul and Robert Ferl in the Epigenetic Adaptation to the Spaceflight Environment – Accumulated Genomic Change Induced by Generations in Space (Plant Habitat-03) investigation which will soon launch to the International Space Station aboard Northrop Grumman’s 18th commercial resupply services mission for NASA. “One of the exciting things about space biology ...
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Surviving Space: Extreme Plant Adaptation

CORE INSTRUMENTS

Gene Expression & Genotyping