Gene Expression & Genotyping

CORE OVERVIEW

Welcome to UF ICBR Gene Expression and Genotyping Core (GE). Our up-to-date gene expression and genotyping analysis methods allow scientists to be on the cutting edge of research. Our high-quality data can help researchers for their grant applications and scientific paper publications.

The Gene Expression & Genotyping core provides state-of-the-art technical services and consultation on Single Cell RNA-Seq, 10x Visium Spatial Gene Expression, Covid-seq, RNA-Seq, 16s metagenomics and ThemoFisher Axiom Genotyping arrays using Beckman Biomek Liquid Handling Automation instrument. High-throughput RNA-seq libraries, 16s libraries and sequencing capture libraries can be done by using the SPT Labtech nanoliter pipetting instrument Mosquito, DrogenFly and Agilent Bravo robot. QuantiGene RNA Assays measure up to 80 gene targets directly with degraded and cross-linked RNA in FFPE tissues and blood, with no RNA purification required. The BioRad QX200 AutoDG Droplet Digital PCR System provides absolute quantification of target DNA or RNA molecules with greater precision and sensitivity than qPCR, the sensitivity off ddPCR System can facilitate expanded analysis of single cells.

To help researchers at every aspect, GE also provides a variety of basic molecular biology services such as DNA isolations, PCR reactions, and PCR purifications for sequencing. High quality of DNA and RNA are the key for reliable data; therefore, DNA and RNA quality assessment services are offered using an Agilent Bioanalyzer and TapeStation instrument at GE.

The walkup service for DNA RNA extraction with QIAcube is available at GE as well.

GE also offer CRISPR services, for more information, please contact us at GeneExpression@ad.ufl.edu

SERVICES OVERVIEW

General Services

Library Construction Services

Single Cell Analysis

Genotyping Services

Project Consultation
Antibody Sequencing
Digital PCR services
Sample Prep: RNA, DNA extraction
RNA DNA QC: Bioanalyzer/TapeStation, Qubit, PicoGreen
CRISPR services
Bravo automation
QuantiGen assays (up to 80 targets): Gene Expression and CNV

16s metagenomics library Automation
Illumina Directional RNA-Seq Library Preparation (Poly A)
Illumina Non-Directional RNA-Seq Library Preparation (Poly A)
Illumina RNA-Seq library Prep for low input, ribo-depletion or degraded RNA
Illumina Small RNA libray w/Barcode
RNA-Seq library automation
rRNAs Depletion
Agilent SureSelect Target Enrichment
IDT Exome Capture Library Construction with/without Pre-pooling
Takara Single Cell Library Construction
16s library Automation Per Sample (batch w/48 samples, 96 samples)
Illumina RNA-Seq libraries (Poly A)
Illumina RNA-Seq library Prep for low input, ribo-depletion or degraded RNA
Illumina RNA-Seq library Prep (polyA with 140 samples and up, 500 samples and up)
Illumina Small RNA-Seq library w/Barcode
NEB Directional RNA-Seq Library Preparation
NEB Non-directional RNA-Seq Library preparation
NextGen Sequencing Library Quantification
RNA-Seq library automation per sample (24 samples, 48 samples, 96 samples)
rRNAs Depletion
RNA Extraction, RNA Clean-up
Agilent SureSelect Target Enrichment
IDT Exome Capture Library Construction with Pre-pooling, without Pre-pooling

10X Genomics Chromium 3’ Single Cell RNA-Seq
10X Genomics Chromium 5’ Single Cell RNA-Seq
10X Genomics Chromium Single Cell CNV analysis
10X Genomics Single Human/Mouse T cell V(D)J Library Prep (per sample)
10X Genomics Chromium Single Cell Feature Barcode for CITE-Seq
10X Genomics Chromium Single Cell Feature Barcode for CRISPR Screening

SNP analysis by qPCR or digital PCR

Looking for data analysis services? Visit ICBR Bioinformatics for services.

Don’t see the service you are looking for? Visit ICBR NextGen DNA Sequencing for more options.

CORE NEWS

ICBR Closed Monday for the 4th of July

ICBR Closed Monday for the 4th of July ICBR will be closed this Monday (7/4) in observation of the 4th of July We will not be accepting packages or samples on Monday. Our normal business hours will resume on Tuesday (7/5). Enjoy the the long weekend and celebrate responsibly! Please refer to our staff directory for additional details. ...

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ICBR attends AGBT 2022 General Meeting!

This June, ICBR attended the Advances in Genome Biology and Technology (AGBT) General Meeting in Orlando, the preeminent genome science and technology conference for top global researchers, leaders and innovators. AGBT delivers a premier experience where heads of labs, institutions, businesses, financial analysts and other high-level stakeholders come together to advance the field and drive game-changing innovation. Significant scientific advances are announced and showcased, science and industr...

In 27,000-acre Preserve, Scientists Begin ‘Age of Discovery’

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Sea turtle conservation gets boost from new DNA detection method

Sea turtle conservation gets boost from new DNA detection method UF News Natalie van Hoose DNA “fingerprints” left behind by sea turtles offer scientists a simple, powerful way of tracking the health and whereabouts of these endangered animals, a key step forward in their conservation. A study led by University of Florida researchers is the first to sequence environmental DNA, or eDNA, from sea turtles — genetic material shed as they travel over beaches and in water. The research project is also...

Important Dates for Fiscal Year End

Please note: Due to the Fiscal Year End, the last invoices to process for FY2022 will be created on Monday, June 13th. Due to Fiscal Year End, ICBR’s upcoming billing schedule will be altered as follows: 6/13/2022 – Last invoices generated in fiscal year 2022 6/23/2022 at noon – Deadline to submit disputes for FY2022 payment. 7/5/2022 – Invoices will begin to be created for FY2023 If you have expiring funds, please work with your core laboratory contact to make sure your project is completed ...

A first: Scientists grow plants in soil from the Moon

A first: Scientists grow plants in soil from the Moon from UF/IFAS by Samantha Murray Scientists have grown plants in soil from the Moon, a first in human history and a milestone in lunar and space exploration. In a new paper published in the journal “Communications Biology,” University of Florida researchers showed that plants can successfully sprout and grow in lunar soil. Their study also investigated how plants respond biologically to the Moon’s soil, also known as lunar regolith, which is r...