Gene Expression & Genotyping

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GENE EXPRESSION & GENOTYPING: Home | Staff & Contact | Services | Instruments | Getting Started | Sample Requirements | Core Resources

Email: ICBR-GeneExpression@ad.ufl.edu | ICBR-Genotyping@ad.ufl.edu Phone: (352) 273-8043 | (352) 273-8036

Overview

The Gene Expression & Genotyping core provides state-of-the-art technical services and consultation on Single Cell RNA-Seq, RNA-Seq, 16s metagenomics and Affymetrix gene expression arrays. High-throughput RNA-seq libraries, 16s libraries and sequencing capture libraries can be done by using the Agilent Bravo robot. QuantiGene RNA Assays measure up to 80 gene targets directly with degraded and cross-linked RNA in FFPE tissues and blood, with no RNA purification required. The BioRad QX200 AutoDG Droplet Digital PCR System provides absolute quantification of target DNA or RNA molecules with greater precision and sensitivity than qPCR, the sensitivty off ddPCR System can facilitate expanded analysis of single cells. The Genotyping services include fragment analysis using AB3730 (96 capillary technology), development of microsatellite libraries using Illumina sequence data, and genotyping using mouse tails or ear punches. These up-to-date gene expression and genotyping analysis methods allow scientists to be on the cutting edge of research.

ICBR Gene Expression & Genotyping Services

Don't see the service you are looking for? Visit ICBR NextGen DNA Sequencing for more options.

General Services

Library Construction Services

Single Cell Analysis

Genotyping Services

Project Consultation
Antibody Sequencing
Digital PCR services
Microarray Services
Sample Prep: RNA, DNA extraction
RNA DNA QC: Bioanalyzer/TapeStation, Qubit, PicoGreen
Sanger Sequencing Load only
CRISPR services
Bravo automation
QuantiGen assays (up to 80 targets): Gene Expression and CNV

16s metagenomics library Automation
Illumina Directional RNA-Seq Library Preparation (Poly A)
Illumina Non-Directional RNA-Seq Library Preparation (Poly A)
Illumina RNA-Seq library Prep for low input, ribo-depletion or degraded RNA
Illumina Small RNA libray w/Barcode
RNA-Seq library automation
rRNAs Depletion
Agilent SureSelect Target Enrichment
IDT Exome Capture Library Construction with/without Pre-pooling
Takara Single Cell Library Construction
16s library Automation Per Sample (batch w/48 samples, 96 samples)
Illumina RNA-Seq libraries (Poly A)
Illumina RNA-Seq library Prep for low input, ribo-depletion or degraded RNA
Illumina RNA-Seq library Prep (polyA with 140 samples and up, 500 samples and up)
Illumina Small RNA-Seq library w/Barcode
NEB Directional RNA-Seq Library Preparation
NEB Non-directional RNA-Seq Library preparation
NextGen Sequencing Library Quantification
RNA-Seq library automation per sample (24 samples, 48 samples, 96 samples)
rRNAs Depletion
RNA Extraction, RNA Clean-up
Agilent SureSelect Target Enrichment
IDT Exome Capture Library Construction with Pre-pooling, without Pre-pooling

10X Genomics Chromium 3’ Single Cell RNA-Seq
10X Genomics Chromium 5’ Single Cell RNA-Seq
10X Genomics Chromium Single Cell CNV analysis
10X Genomics Single Human/Mouse T cell V(D)J Library Prep (per sample)
10X Genomics Chromium Single Cell Feature Barcode for CITE-Seq
10X Genomics Chromium Single Cell Feature Barcode for CRISPR Screening

Fragment Analysis
STR Analysis
Cell Line Authentication
Copy Number Variance
SNP analysis by qPCR or digital PCR

Please note that our library construction and sequencing services do not include data analysis. Visit ICBR Bioinformatics to request Data Analysis services.

Core News

ICBR Faculty Director to Speak at 2019 Florida Genetics Symposium

We are excited to announce that Sixue Chen, PhD will present at the 2019 Florida Genetics Symposium, presented by the University of Florida Genetics Institute. This two day event on November 4th and 5th will bring together some of the brightest minds in genetics. Faculty Director of the ICBR Proteomics & Mass Spectrometry Core, Dr. Chen will present “Single Cell-Type Proteomics and Metabolomics: New Insights into Guard Cell Umminuty and CO2 Responses” on Monday, November 4th from 1:45pm ...

The Beast is Here! ICBR Launches the NovaSeq 6000 DNA Sequencer

Dr. Steven Madore, ICBR Associate Director for Science and UFHCC Associate Director for Shared Resources, next to the new NovaSeq6000 (“the Beast“) with ICBR NGS Core Scientific Director Dr. David Moraga. The ICBR NextGen DNA Sequencing Core provides cutting edge instrumentation and technical expertise required to support campus-wide research activites. Established in 1987, the NGS Core has contributed to significant scientific discoveries on campus. For example, did you know that the first...

The Luminex FLEXMAP 3D® System – A Multiplexed Analytical Platform for Novel Biomarker Discovery

Figure 1. Luminex bead architecture technology ICBR Proteomics & Mass Spectrometry (PM) Core is now offering access to the Luminex FLEXMAP 3D® analytical platform for discovering and validation of protein biomarkers across a wide range of diseases. The FLEXMAP 3D® system combines differentially dyed fluorescent microsphere sets with an innovative instrument design to enable simultaneous measurement of the concentration of a large number of different analytes in a rapid multiplexed as...

Dr. Anna-Lisa Paul receives NASA’s Exceptional Scientific Achievement Medal

Dr. Anna-Lisa Paul receives NASA's Exceptional Scientific Achievement Medal

Congratulations to UF’s Dr. Anna-Lisa Paul on receiving the NASA Exceptional Scientific Achievement Medal this past September! The prestigious award is reserved for individual efforts that are in line with NASA’s mission and have resulted in considerable scientific discovery or resulted in contributions of fundamental importance in their field or significantly improved the understanding of their field. Dr. Paul is the Interim Director at UF ICBR and Research Professor in the UF Horticultural...

Introducing Core Directors’ Open Consultation Meetings

Introducing Core Directors' Open Consultation Meetings

In a continued effort to provide accessible and specialized expertise to our users, UF ICBR is proud to announce our Core Directors’ Open Consultation Meetings. Each month we will be available for a two hour walk-in session to discuss the best approaches and strategies for using ICBR technologies and expertise to drive your research forward. Our first meeting will be on Monday, October 7th from 11:00am to 1:00pm in CGRC 189. No appointment is require nor do you have to be a current ICBR user t...

The NovaSeq 6000 has arrived at UF ICBR!

After much anticipation, the Illumina NovaSeq 6000 has arrived at UF ICBR. Users can expect access later this fall. The NovaSeq 6000 Sequencing System unleashes groundbreaking innovations that leverage our proven technology. Now you can get scalable throughput and flexibility for virtually any sequencing method, genome, and scale of project. The NovaSeq will be housed in our NextGen DNA Sequencing core. Stay tuned for more information and events this fall. For more information on the instrument...

ICBR Bioinformatics Contributes to Landmark UFHCC Publication

The ICBR Bioinformatics Core contributed to a high-profile paper from the cancer research laboratory of UFHCC Director Dr. Jonathan Licht, recently published in Cancer Discovery. The paper, entitled “A Mutation in Histone H2B Represents a New Class of Oncogenic Driver”, describes how mutations in core histones disrupt nucleosome stability, leading to disregulation of gene expression and acting as a novel oncogenic mechanism. As part of this study the investigators used a technique ca...

Help ICBR Grow in Ten Quick Questions!

We want to hear about your experience working with UF ICBR. What do you like? How can we improve? We want to hear from you. For your convenience, we kept our survey to ten quick questions. This is your opportunity to help us continue our quest to become the best resource on campus. Click Here to Participate in our Survey ...