Gene Expression & Genotyping

Getting Started
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Getting Started

How do I get Started?

If you are clear on what services you are requesting, please follow the following steps to get started, otherwise, schedule a meeting with core director (yanp@ufl.edu) for a consultation meeting.

  1. Create an ilab account
  2. Follow these guides for creating a specific service request:
  3. Read sample submission guidelines
  4. Bring samples to CGRC 178 B, or ship it to:

                Attn:

                Gene Expression & Genotyping/Service Name (example: RNA-Seq)

                CGRC 178 B

                Gainesville Fl 32610

Sample Submission Requirement

  • When samples are submitted they should be labeled with Sample IDs. These Sample IDs are associated with their sample names listed on the excel sample information sheet.
  • Please submit the samples in RNAase DNAase-free 8-tube strips or 96-well plate if you have more than 16 samples..
  • If you have less than 8 samples, you can submit in RNAase DNAase-free 1.5 ml microfuge tubes having numerical ID (e.g., 1, 2, 3….)

Sample Drop-Off Instruction

  • 10X Genomics Single Cell: Scheduling is needed. We are not able to process if it is not scheduled. We prefer to receive samples in morning time/as early as possible so that we can complete certain process to a ‘save stop’ point in the same day.
  • RNA-Seq, Microarray and 16s Metogenomics: Room 178B (In person or shipment on dry ice)
  • DNA RNA QC: please drop off in the refrigerator in Room 185 (please fill out the submission sheet next to the refrigerator)
  • Fragment Analysis: Drop off in refrigerator in 185 or ship with ice pack (please fill out the submission sheet next to the refrigerator)

Sample Shipping Instruction

Outside of the UF network or off-campus samples are to be shipped Monday-Thursday and delivered by domestic overnight courier service for express next day delivery. Please ship DNA samples on ice packs and RNA samples on dry ice. Samples must be in nuclease-free 1.5 mL Eppendorf tubes and sealed with Para film to minimize sample loss. The shipment must include the following: Printed copy of the Sample information which includes Sample ID (Numerical, e.g., 1, 2, 3…etc.), QC results (e.g., Nano Drop conc) etc.

Please ship samples to:

UF ICBR, Gene Expression & Genotyping Core

2033 Mowry Road, CGRC Room 178B

University of Florida

Gainesville, FL 32610

Attn: Yanping Zhang/Service Name