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The service includes cloning and sequencing mouse heavy and light chains starting with total RNA, alignments of the heavy and light chains, to a detailed project report with annotated antibody DNA, protein sequences and CDRs.
Bravo 16s and RNAseq Library Automation (Bravo Automations)
16s ribosomal RNA sequencing can be used to identify and compare bacteria present within a given sample.
RNA-Seq is a powerful research tool for expression and variant analysis. Its’ application includes quantifying mRNA abundance; determining the transcriptional structure of genes: start sites, 5′ and 3′ ends, and splicing patterns; comparably detecting the changing expression levels of each transcript during development and under different conditions. It picks up all sequences that are present in the cell, regardless of their annotation status.
Gene Expression Core offers library preparation automation by Agilent Bravo Automation Workstation.The constructed libraries can be run on all NextGen sequencing platforms: Illumina HiSeq, MiSeq, and NextSeq. Sequence can be done by ICBR NextGen Sequencing Core.
We also offer Agilent’s SureSelect Target Enrichment platform which allows you to focus your NextGen sequencing workflow on key genomic regions of interest while reducing cost per sample. Other NextGen relative services include cDNA library construction, normalization and rRNA depletion (Ribominus). If requested, we will directly deliver the libraries to our ICBR NextGen Sequencing Core with an e-mail notification to you. Otherwise the library will be returned to you.
Custom Bravo Run (Bravo Automations)
The service includes the Bravo consumables and run assistance.
For NEW customers and NEW projects: Schedule a consultation to discuss experimental design and prepare a project outline with an ICBR staff member.
This service allows the customer to do absolute quantification, rare allele detection, quantification using TaqMan SNP array and CNV (Copy Number Variation) in a more sensitive, specific, and precise way.
Agilent 2100 Bioanalyzer/2200 TapeStation Analysis (RNA DNA QC)
**Attention: If you are submitting libaries for a sequencing run in the NextGen core, please request their library evaluation services**
The Agilent 2100 Bioanalyzer and 2200 TapeStation system automatically perform the multiple steps of gel-based electrophoresis, replacing the need to run agarose gels to separate and quantitate your RNA or DNA samples. A software tool, RNA Integrity Number (RIN) can help scientists estimate the integrity of total RNA samples. Data is returned in the form of PDF.
Sample Concentration Measurement (RNA DNA QC)
Quantitate micro-volume of samples via NanoDrop, or Qubit. Please provide the DNA size if caculated nM concentration is needed.
RNA-Seq is a powerful research tool for expression and variant analysis. Its’ application includes quantifying mRNA abundance; determining the transcriptional structure of genes; start sits, 5′ and 3′ ends, and splicing patterns; comparably detecting the changing expression levels of each transcript during development and under different conditions. It picks up all sequences that are present in the cell, regardless of their annotation status.
Service includes gDNA/RNA extraction, RNA clean up, PCR purification, revers transcription, ds cDNA synthesis, TA cloning and more
The 10x Genomics Chromium Controller System
The 10X Genomics Chromium Controller instrument is designed to capture thousands of single cells or DNA fragment, and combined them with reagents and gel bead (which contains barcoded oligonucleotides) in emulsion droplets (GEMs) to perform a variety of assays. Each GEM are uniquely barcoded.
It can perform Single Cell RNA-seq, Single Cell Copy Number Variation Profiling, Single Cell Immune Repertoire Profiling, Single Nuclei Expression Profiling, and Single Cell CITE-seq. For those assays, it can capture 500-10,000 individual cells per sample. Additionally, it partitions high molecular weight (HMW) DNA into individual GEMs to capture long-range information using Illumina sequencer. This assay requires low input of high molecular weight DNA to produce “linked read” information, which can be used for assembling genomes, assessing structural variants and obtaining genome phase information.
General Service (qPCR)
qPCR analysis offered at Gene Expression and Genotyping are: SYBR® or TaqMan™ reagents allowing absolute, relative, relative standard or comparative Ct quantification. Services includes but not limited to qPCR Absolute Quantification Data Analysis/Per Standard Curve; RT Profiler PCR arrays – chemistry; qPCR post amplification read (SNP); qPCR Mid service on CFX (1 plate); qPCR Plate set-up for 384 well Absolute Quantitation; qPCR primer and probe sets assay design.
Quantification of NextGen Sequencing libraries by qPCR
This method is widely used because its ability to target adapter sequences for a specific sequences in NextGen libraries. It enables accurate quantification of NextGen libraries. It measures the functional molecular of the libraries.
SYBR Green-based Detection (qPCR)
This real-time PCR assay uses SYBR Green Chemistry to detect PCR products as it accumulate during real time PCR process.
TaqMan-based Detection (qPCR)
This real-time PCR assay consists a target-specific probe and two gene specific primes that can increase the specificity of quantification PCR. The fluorescence single generated by probe allows quantitate measurements of the accumulated PCR product and increases the specificity of gene detection.
Services | UF | Non-Profit | Commercial | Details |
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Chip Hybridization and Scanning | $60 | $69 | $75 | Customer provides labeled cRNA. Service includes hybridization, wash, and scan of the chip along with delivery of the raw data. |
Affymetrix miRNA Array | $115 | $135 | $145 | Service includes total RNA QC with Bioanalyzer, labeling, hybridization, wash, scan, and raw data delivery. Customer provides own arrays. |
Affymetrix Labeling: 10+ Samples | $160 | $180 | $200 | Service with bulk rate discount includes total RNA QC with Bioanalyzer, cDNA synthesis, labeling, hybridization, wash, scan, and raw data delivery. Customer provides own reagents and arrays. |
Affymetrix Labeling: 1-9 Samples | $190 | $210 | $230 | Service includes reagents but not arrays, total RNA QC with Bioanalyzer, cDNA synthesis, labeling, hybridization, wash, scan, and raw data delivery. |
Affymetrix GeneChip Chemistry: 10+ Samples | $210 | $230 | $250 | Service includes reagents, processing of Affymetrix GeneST, exon, HTA arrays starting with total RNA and raw data delivery. Bulk discount for 10 or more samples. |
Affymetrix GeneChip Chemistry: 1-9 Samples | $225 | $245 | $265 | Services includes processing Affymetrix GeneST, exon, HTA arrays starting with total RNA, and raw data delivery. Customer provides own arrays. |
Services | UF | Non-Profit | Commercial | Details |
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Agilent SureSelect Target Enrichment | $180 | $220 | $300 | Service includes hybridized indexed gDNA libraries with biotinylated RNA baits, select targeted regions using magnetic streptavidin beads, and amplification of the libraries. |
Services | UF | Non-Profit | Commercial | Details |
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Antibody Sequencing (VH VL domain) | $750 | $850 | $980 | The service includes cloning and sequencing mouse heavy and light chains starting with total RNA, alignments of the heavy and light chains, to a detailed project report with annotated antibody DNA, protein sequences and CDRs. There will be extra $50 charge for RNA extraction if you submit hybridoma cell pellets (1X106). |
Services | UF | Non-Profit | Commercial | Details |
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16 s library Automation Per Sample (batch w/48 samples) | $74 | $83.25 | $92.50 | |
16 s library Automation Per Sample (batch w/96 samples) | $69 | $77.63 | $86.25 | |
AmliSeq library Automation (>384 samples) | $105 | $107 | $110 | Service includes preparation, pooling of barcoded libraries to one single library, and loading on Ion Torrent chip for sequencing. |
AmliSeq library Automation (<192 samples) | $200 | $250 | $324 | Service includes preparation, pooling of barcoded libraries to one single library, and loading on Ion Torrent chip for sequencing. |
AmliSeq library Automation (193-384 samples) | $160 | $205 | $279 | Service includes preparation, pooling of barcoded libraries to one single library, and loading on Ion Torrent chip for sequencing. |
AmpureBead Clean up Per Run (batch w/32 samples, Ampure not included) | $117.58 | $132.28 | $146.98 | |
AmpureBead Clean up Per Run (batch w/48 samples, Ampure not included) | $128.76 | $144.86 | $160.95 | |
AmpureBead Clean up Per Run (batch w/96 samples, Ampure not included) | $162.30 | $182.59 | $202.88 | |
AmpureBead Clean up Per Run w/24 samples, Ampure not included) | $106.40 | $119.70 | $133 | |
DNASeq library automation per sample (24 samples) | $165 | $215 | $289 | Service includes DNASeq library preparation by Agilent's Bravo Automated Liquid Handling platform for 24 samples. Final pooled library will be delivered to Sequencing facilities or customer. Automating sequencing library preparation minimizes errors, reduces hands-on time, and enables higher throughput. |
DNASeq library automation per sample (48 samples) | $160 | $210 | $284 | Service includes DNASeq library preparation by Agilent's Bravo Automated Liquid Handling platform for 44-48 samples. Final pooled library will be delivered to Sequencing facilities or customer. Automating sequencing library preparation minimizes errors, reduces hands-on time, and enables higher throughput. |
DNASeq library automation per sample (96 samples) | $150 | $200 | $274 | Service includes DNASeq library preparation by Agilent's Bravo Automated Liquid Handling platform for 89-96 samples. Final pooled library will be delivered to Sequencing facilities or customer. Automating sequencing library preparation minimizes errors, reduces hands-on time, and enables higher throughput. |
Primer transfer from 96-well to 384-well plate Per Run w/up to 10 plates | $78.50 | $88.31 | $98.13 | |
Primer transfer from 96-well to 384-well plate Per Run w/up to 20 plate | $96 | $108 | $120 | |
Primer transfer from 96-well to 384-well plate Per Run w/up to 5 plates | $67.50 | $75.94 | $84.38 | |
RNA-Seq library automation per sample (24 samples) | $220 | $270 | $344 | Service include RNA-Seq library preparation by Agilent's Bravo Automated Liquid Handling platform for 24 samples. Final pooled library will be delivered to Sequencing facilities or customer. Automating sequencing library preparation minimizes errors, reduces hands-on time, and enables higher throughput. |
RNA-Seq library automation per sample (48 samples) | $200 | $250 | $324 | Service include RNA-Seq library preparation by Agilent's Bravo Automated Liquid Handling platform for 44-48 samples. Final pooled library will be delivered to Sequencing facilities or customer. Automating sequencing library preparation minimizes errors, reduces hands-on time, and enables higher throughput. |
RNA-Seq library automation per sample (96 samples) | $175 | $225 | $299 | Service include RNA-Seq library preparation by Agilent's Bravo Automated Liquid Handling platform for 89-96 samples. Final pooled library will be delivered to Sequencing facilities or customer. Automating sequencing library preparation minimizes errors, reduces hands-on time, and enables higher throughput. |
Services | UF | Non-Profit | Commercial | Details |
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(CR001) CRISPR Project consultation (1 hour) | $0 | $0 | $0 | |
(CR002) CRISPR Project consultation (additional hours) | $50 | $57.50 | $62.50 | |
(CR003) Cell Line Optimization for Transduction or Nucleofection | $50 | $57.50 | $62.50 | This work will determine the optimal conditions for transduction or nucleofection of a lentivirus construct for CRISPR modification in a particular cell line. The process will include cell culture, viral preparation and packaging, transfection and viral titration to determine the optimal multiplicity of infection (MOI) for transduction of nucleofection. |
(CR004) CRISPR Single Cell KO in Cell Line Pool Using 1 sgRNA | $400 | $460 | $500 | This work will generate a pool of CRISPR knock outs of a single gene in a mammalian cell line using 1 sgRNA. Please note that the individual targeting success of any 1 sgRNA can be difficult to predict so we recommend using three different sgRNA (CR005) if a particular sgRNA has not previously been validated. The process includes the design and synthesis of 1 sgRNA, cell culture, viral preparation and packaging, transfection, viral titration and Puromycin selection. Knock out success will be evaluated by T7 endonuclease assay and in vitro Cas9 assay. Requester will receive a heterogeneous pool of cells containing the CRISPR targeted gene KO. Please note that the pool contains multiple different KO clones as each targeting event and repair results in an individual genetic modification. Individual knockout clones can be isolated from the pool as described in CR006 and CR007. |
(CR005) CRISPR Single Cell KO in Cell Line Pool Using 3 sgRNAs (Recommended) | $1000 | $1150 | $1250 | This work will generate three pools of CRISPR knock outs of a single gene in a mammalian cell line using 3 sgRNAs. Please note that the individual targeting success of any 1 sgRNA can be difficult to predict so we recommend using three different sgRNA if a particular sgRNA has not previously been validated. The process includes the design and synthesis of 3 sgRNA, cell culture, viral preparation and packaging, transfection, viral titration and Puromycin selection. Knock out success for each sgRNA in the pool will be evaluated by T7 endonuclease assay and in vitro Cas9 assay. Requester will receive a heterogeneous pool of cells containing the CRISPR targeted gene KO for each sgRNA. Please note that the pool contains multiple different KO clones as each targeting event and repair results in an individual genetic modification. Individual knockout clones can be isolated from the pool as described in CR006 and CR007. |
(CR006) Generation of CRISPR Single Gene KO Stable Cell Line Using 3 sgRNAs [3 Clonal Isolates per Gene] | $2500 | $2875 | $3125 | Three individual validated knockout clones will be isolated from the CRISPR KO pools generated in CR004/CR005 for each sgRNA by limiting dilution cloning or other approaches depending on the cell line. Each isolated clone will be validated by T7 endonuclease assay and in vitro Cas9 assay. |
(CR008) Vector-Free CRISPR Single Gene KO Stable Cell Line Using Nucleofection of Cas9 Protein and 3 sgRNA [3 Clonal Isolates] | $2100 | $2415 | $2625 | No viral vector is used. Ribonucleoprotein (RNP) complex of Cas9 protein and sgRNA are directly introduced into the cell line of interest to generate a single gene knock out stable cell line for the desired gene. The process includes the design and synthesis of 1 sgRNA, sgRNA and Cas9 protein complex formation (RNP complexing), and nucleofection of the RNP into the cell line to generate heterogenous pool of Knockout clones. Overall knockout efficiency will be determined by T7 endonuclease assay and in vitro Cas 9 assay. Three individual knockout clones will be isolated from the CRISPR knockout pools by limiting dilution cloning or other approaches depending on the cell line. Each isolated clone will be validated by T7 endonuclease assay and in vitro Cas 9 assay. |
(CR009) Vector-Free CRISPR Single Gene KO Cell Line Pool Using Nucleofection of Cas9 Protein and 1 sgRNA [3 Clonal Isolates] | $700 | $805 | $875 | No viral vector is used. Ribonucleoprotein (RNP) complex of Cas9 protein and sgRNA are directly introduced into the cell line of interest to generate a single gene knock out stable cell line for the desired gene. The process includes the design and synthesis of 3 sgRNA, sgRNA and Cas9 protein complex formation (RNP complexing), and nucleofection of the RNP into the cell line to generate heterogenous pool of Knockout clones. Overall knockout efficiency will be determined by T7 endonuclease assay and in vitro Cas 9 assay. Three individual knockout clones for each sgRNA will be isolated from the CRISPR knockout pools by limiting dilution cloning or other approaches depending on the cell line. Each isolated clone will be validated by T7 endonuclease assay and in vitro Cas 9 assay. |
(CR100-000) CRISPR Whole Genome or Targeted Genome Screening-Consultation | $0 | $0 | $0 | Required no charge consultation for all CRISPR-based screening projects. |
(CR100-111) CRISPR Genome Screening -1 chemical at 1 dose at 1 time point | $2995 | $3445 | $3745 | Screening of 1 chemical at 1 dose for 1 time point. Performed in triplicate. Please order Bioinformatics Data analysis and NGS NEXTSEQ 500 1 x 75 services separately at additional costs. |
(CR100-112) CRISPR Genome Screening -1 chemical at 1 dose at 2 time points | $4470 | $5140 | $5585 | Screening of 1 chemical at 1 dose for 2 time points. Performed in triplicate. Please order Bioinformatics Data analysis and NGS NEXTSEQ 500 1 x 75 services separately at additional costs. |
(CR100-113) CRISPR Genome Screening -1 chemical at 1 dose at 3 time points | $5945 | $6840 | $7430 | Screening of 1 chemical at 1 dose for 3 time points. Performed in triplicate. Please order Bioinformatics Data analysis and NGS NEXTSEQ 500 1 x 75 services separately at additional costs. |
(CR100-121) CRISPR Genome Screening -1 chemical at 2 doses at 1 time point | $4170 | $4795 | $5215 | Screening of 1 chemical at 2 doses for 1 time point. Performed in triplicate. Please order Bioinformatics Data analysis and NGS NEXTSEQ 500 1 x 75 services separately at additional costs. |
(CR100-122) CRISPR Genome Screening -1 chemical at 2 doses at 2 time points | $6030 | $6935 | $7535 | Screening of 1 chemical at 2 doses for 2 time points. Performed in triplicate. Please order Bioinformatics Data analysis and NGS NEXTSEQ 500 1 x 75 services separately at additional costs. |
(CR100-123) CRISPR Genome Screening -1 chemical at 2 dose at 3 time points | $7890 | $9075 | $9865 | Screening of 1 chemical at 2 doses for 3 time points. Performed in triplicate. Please order Bioinformatics Data analysis and NGS NEXTSEQ 500 1 x 75 services separately at additional costs. |
(CR100-131) CRISPR Genome Screening -1 chemical at 3 doses at 1 time point | $5345 | $6145 | $6680 | Screening of 1 chemical at 3 doses for 1 time point. Performed in triplicate. Please order Bioinformatics Data analysis and NGS NEXTSEQ 500 1 x 75 services separately at additional costs. |
(CR100-132) CRISPR Genome Screening -1 chemical at 3 doses at 2 time points | $7205 | $8285 | $9005 | Screening of 1 chemical at 3 doses for 2 time points. Performed in triplicate. Please order Bioinformatics Data analysis and NGS NEXTSEQ 500 1 x 75 services separately at additional costs. |
(CR100-133) CRISPR Genome Screening -1 chemical at 3 doses at 3 time points | $9065 | $10425 | $11335 | Screening of 1 chemical at 3 doses for 3 time points. Performed in triplicate. Please order Bioinformatics Data analysis and NGS NEXTSEQ 500 1 x 75 services separately at additional costs. |
(CR100-211) CRISPR Genome Screening -2 chemicals at 1 dose at 1 time point | $3515 | $4045 | $4395 | Screening of 2 chemicals at 1 dose for 1 time point. Performed in triplicate. Please order Bioinformatics Data analysis and NGS NEXTSEQ 500 1 x 75 services separately at additional costs. |
(CR100-212) CRISPR Genome Screening -2 chemicals at 1 dose at 2 time points | $5375 | $6180 | $6720 | Screening of 2 chemicals at 1 dose for 2 time points. Performed in triplicate. Please order Bioinformatics Data analysis and NGS NEXTSEQ 500 1 x 75 services separately at additional costs. |
(CR100-213) CRISPR Genome Screening -2 chemicals at 1 dose at 3 time points | $7235 | $8320 | $9045 | Screening of 2 chemicals at 1 dose for 3 time points. Performed in triplicate. Please order Bioinformatics Data analysis and NGS NEXTSEQ 500 1 x 75 services separately at additional costs. |
(CR100-221) CRISPR Genome Screening -2 chemicals at 2 doses at 1 time point | $5045 | $5800 | $6035 | Screening of 2 chemicals at 2 dose2 for 1 time point. Performed in triplicate. Please order Bioinformatics Data analysis and NGS NEXTSEQ 500 1 x 75 services separately at additional costs. |
(CR100-222) CRISPR Genome Screening -2 chemicals at 2 doses at 2 time points | $7290 | $8385 | $9115 | Screening of 2 chemicals at 2 doses for 2 time points. Performed in triplicate. Please order Bioinformatics Data analysis and NGS NEXTSEQ 500 1 x 75 services separately at additional costs. |
(CR100-223) CRISPR Genome Screening -2 chemicals at 2 doses at 3 time points | $9540 | $10975 | $11925 | Screening of 2 chemicals at 2 doses for 3 time points. Performed in triplicate. Please order Bioinformatics Data analysis and NGS NEXTSEQ 500 1 x 75 services separately at additional costs. |
(CR100-231) CRISPR Genome Screening -2 chemicals at 3 doses at 1 time point | $5530 | $6360 | $6915 | Screening of 2 chemicals at 3 doses for 1 time point. Performed in triplicate. Please order Bioinformatics Data analysis and NGS NEXTSEQ 500 1 x 75 services separately at additional costs. |
(CR100-232) CRISPR Genome Screening -2 Chemicals at 3 doses at 2 time points | $8165 | $9390 | $10206 | Screening of 2 chemicals at 3 doses for 2 time points. Performed in triplicate. Please order Bioinformatics Data analysis and NGS NEXTSEQ 500 1 x 75 services separately at additional costs. |
(CR100-233) CRISPR Genome Screening -2 chemicals at 3 doses at 3 time point | $10800 | $12420 | $13500 | Screening of 2 chemicals at 3 doses for 3 time points. Performed in triplicate. Please order Bioinformatics Data analysis and NGS NEXTSEQ 500 1 x 75 services separately at additional costs. |
(CR100-311) CRISPR Genome Screening -3 chemicals at 1 dose at 1 time point | $4040 | $4646 | $5050 | Screening of 3 chemicals at 1 dose for 1 time point. Performed in triplicate. Please order Bioinformatics Data analysis and NGS NEXTSEQ 500 1 x 75 services separately at additional costs. |
(CR100-312) CRISPR Genome Screening -3 chemicals at 1 dose at 2 time points | $5900 | $6785 | $7375 | Screening of 3 chemicals at 1 dose for 2 time points. Performed in triplicate. Please order Bioinformatics Data analysis and NGS NEXTSEQ 500 1 x 75 services separately at additional costs. |
(CR100-313) CRISPR Genome Screening -3 chemicals at 1 dose at 3 time points | $7765 | $8930 | $9705 | Screening of 3 chemicals at 1 dose for 3 time points. Performed in triplicate. Please order Bioinformatics Data analysis and NGS NEXTSEQ 500 1 x 75 services separately at additional costs. |
(CR100-321) CRISPR Genome Screening -3 chemicals at 2 doses at 1 time point | $5540 | $6371 | $6925 | Screening of 3 chemicals at 2 dose2 for 1 time point. Performed in triplicate. Please order Bioinformatics Data analysis and NGS NEXTSEQ 500 1 x 75 services separately at additional costs. |
(CR100-322) CRISPR Genome Screening -3 chemicals at 2 doses at 2 time points | $8175 | $9400 | $10220 | Screening of 3 chemicals at 2 doses for 2 time points. Performed in triplicate. Please order Bioinformatics Data analysis and NGS NEXTSEQ 500 1 x 75 services separately at additional costs. |
(CR100-323) CRISPR Genome Screening -3 chemicals at 2 doses at 3 time points | $10810 | $12430 | $13515 | Screening of 3 chemicals at 2 doses for 3 time points. Performed in triplicate. Please order Bioinformatics Data analysis and NGS NEXTSEQ 500 1 x 75 services separately at additional costs. |
(CR100-331) CRISPR Genome Screening -3 chemicals at 3 doses at 1 time point | $6025 | $6929 | $7531 | Screening of 3 chemicals at 3 doses for 1 time point. Performed in triplicate. Please order Bioinformatics Data analysis and NGS NEXTSEQ 500 1 x 75 services separately at additional costs. |
(CR100-332) CRISPR Genome Screening -3 chemicals at 3 doses at 2 time points | $9050 | $10407 | $11315 | Screening of 3 chemicals at 3 doses for 2 time points. Performed in triplicate. Please order Bioinformatics Data analysis and NGS NEXTSEQ 500 1 x 75 services separately at additional costs. |
(CR100-333) CRISPR Genome Screening -3 chemicals at 3 doses at 3 time point | $12075 | $13886 | $15100 | Screening of 3 chemicals at 3 doses for 3 time points. Performed in triplicate. Please order Bioinformatics Data analysis and NGS NEXTSEQ 500 1 x 75 services separately at additional costs. |
Services | UF | Non-Profit | Commercial | Details |
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Data Analysis | $50 | $57.50 | $62.50 | Service includes array analysis, p-value, FDR and fold change. |
Services | UF | Non-Profit | Commercial | Details |
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Digital PCR Drop-off Service | $25 | $28 | $45 | Service includes starting with ready to run BioRad digital PCR plate to raw data file delivery. Process includes droplet generation, PCR amplification, read the plate reader and raw data file delivery. |
Digital PCR Droplet Auto Generation/Plate Reader walk up service per EXTRA 8 reactions | $55 | $62 | $77 | Service includes the 1st 8 rections for the drop off plate |
Digital PCR Droplet Auto Generation/Plate Reader walk up servicefor the 1st 8 rections | $67 | $75 | $94 | Service includes the 1st 8 rections for the drop off plate |
Digital PCR full service for the 1st 8 reactions | $97 | $109 | $121 | Service includes the 1st 8 rections set up and date delivery as excel format for BioRad digital PCR analysis with primers/assays provided by customer |
Digital PCR full service per EXTRA 8 reactions | $87 | $98 | $109 | Service includes every extra 8 rections set up and date delivery as excel format for BioRad digital PCR analysis with primers/assays provided by customer |
Illumina Library quantification by digital PCR (per sample) | $45 | $50 | $56 | NextGen Library quantification by BioRad Digital PCR system. |
Services | UF | Non-Profit | Commercial | Details |
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Consultation and data interpretation (1 hour) | $50 | $75 | $100 | Service includes one hour of assisting customers in understanding their qPCR data. |
Services | UF | Non-Profit | Commercial | Details |
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Bioanalyzer run for DNA RNA QC (up to 11/12 samples) | $71 | $96 | $126 | Service includes RNA and DNA quality assessment, reagents, chip, and ladder for Nano, Pico, DNA 1000, or DNA 12000. Due to increased reagent cost, the Bioanalyzer/TapeStation service fees is increased accordingly starting Jan 2, 2018. Please check the service fee before you submit your RNA/DNA QC samples. |
DNA High Sensitivity Bioanalyzer (up to 11 samples) | $99 | $123 | $126 | Service includes fragmented DNA, NextGen libray quality assessment with concentrations as low as 5 pg/µL. 11samples/per chip. Due to increased reagent cost, the Bioanalyzer/TapeStation service fees is increased accordingly starting Jan 2, 2018. Please check the service fee before you submit your RNA/DNA QC samples. |
DNA High Sensitivity TapeStation per plate ( 96 samples) for DNA, Fragment, NextGen library QC | $582 | $782 | $962 | DNA High Sensitivity TapeStation per plate (96 samples) for DNA Fragment, NextGen library QC: High Sensitivity DNA ScreenTape per plate service for DNA fragment and NextGen libraries with the sample concentration of 10-1000pg/ul. |
DNA High Sensitivity TapeStation/Bioanalyzer run (per sample) for DNA Fragment, NextGen library QC | $10.20 | $13.20 | $18.20 | Service includes fragmented DNA, NextGen library quality assessment with concentrations as low as 5pg/ul. Due to increased reagent cost, the Bioanalyzer/TapeStation service fees is increased accordingly starting Jan 2, 2018. Please check the service fee before you submit your RNA/DNA QC samples. |
DNA RNA QC by TapeStation/Bioanalyzer (per sample) | $7.20 | $10.20 | $16.20 | Service includes RNA and DNA quality assessment, reagents, chip, and ladder for Nano, Pico, DNA 1000, or DNA 12000. Minimum of 7 samples to run chip. If submitting less than 7 samples, customer will have to wait until we have at least 7 samples to fill up the chip. Due to increased reagent cost, the Bioanalyzer/TapeStation service fees is increased accordingly starting Jan 2, 2018. Please check the service fee before you submit your RNA/DNA QC samples. |
NanoDrop: Samples #1-8 | $3 | $5 | $8 | Customer provides at least 2ul/per sample for Nanodrop. |
NanoDrop: Samples #17-24 | $7 | $9 | $12 | Customer provides at least 2ul/per sample for Nanodrop. |
NanoDrop: Samples #25-32 | $9 | $11 | $14 | Customer provides at least 2ul/per sample for Nanodrop. |
NanoDrop: Samples #9-16 | $5 | $7 | $10 | Customer provides at least 2ul/per sample for Nanodrop. |
PicoGreen Assay: 1-16 Samples | $38 | $57 | $75 | Service includes quantitation assay of DNA and RNA for 1-16 Samples. |
PicoGreen Assay: 17-40 Samples | $50 | $75 | $75 | Service includes quantitation assay of DNA and RNA for 17-40 Samples. |
Small RNA Bioanalyzer | $85 | $110 | $135 | Service includes resolving small nucleic acids in the size range of 6 to 150 nt. 11 samples/ per chip. Due to increased reagent cost, the Bioanalyzer/TapeStation service fees is increased accordingly starting Jan 2, 2018. Please check the service fee before you submit your RNA/DNA QC samples. |
TapeStation per plate (96 samples) | $552 | $752 | $932 | TapeStation per plate (96 samples): DNA ScreenTape per plate service for DNA fragment and NextGen libraries with the sample concentration of 1-50ng/ul, or RNA ScreenTape per plate service with the sample concentration of 25-500ng/ul. |
Services | UF | Non-Profit | Commercial | Details |
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Qubit Quantification (one charge for every 1-10 samples) | $20 | $23 | $25 | Service includes quantification of DNA and RNA samples by using Qubit. Cost of reagents included. |
Services | UF | Non-Profit | Commercial | Details |
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cDNA library construction and normalization | $1000 | $1500 | $2200 | Service begins with total RNA to normalized cDNA library, to limit the sequencing read of abundant genes. |
IDT Exome Capture Library Construction with Pre-pooling of 3 or less (per sample) | $330 | $370 | $420 | IDT Exome Capture Library Construction with Pre-pooling of 3 or less (per sample) |
IDT Exome Capture Library Construction with Pre-pooling of 4 and up (per sample) | $300 | $340 | $390 | IDT Exome Capture Library Construction with Pre-pooling of 4 and up (per sample) |
IDT Exome Capture Library Construction Without Pre-pooling (per sample) | $370 | $410 | $460 | IDT Exome Capture Library Construction Without Pre-pooling (per sample) |
Illumina RNA-Seq libraries (Poly A) | $150 | $187 | $210 | Service includes total RNA QC with Bioanalyzer, library construction, library QC, and library delivery. There will be extra $50 if starts with cell pellet or tissue samples. |
Illumina RNA-Seq library Prep for low input, ribo-depletion or degraded RNA | $220 | $275 | $299 | Service starts with low input total RNA OR ribo depletion followed by library construction, library QC and library delivery to customers or NextGen for sequencing. |
Illumina RNA-Seq library Prep (polyA with 140 samples and up) | $140 | $175 | $196 | |
Illumina RNA-Seq library Prep (polyA with 500 samples and up) | $120 | $150 | $168 | |
Illumina Small RNA libray w/Barcode | $300 | $354 | $375 | Service includes total RNA QC with Bioanalyzer, library construction, library QC, and library delivery. |
NEB RNA-Seq Library preparation (kit provided) | $165 | $200 | $250 | Service includes total RNA QC with Bioanalyzer, directional library construction, library QC, and library delivery. |
rRNAs Depletion | $160 | $175 | $200 | Service includes removal of human, mouse, rat, bacteria, and plant ribosomal RNA. |
Services | UF | Non-Profit | Commercial | Details |
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Affymetrix Reagents | $85 | $85 | $85 | |
GA-G05 Miscellaneous | $0 | $0 | $0 | Service includes purchase of customer's kits, supplies, reagents, etc. |
Generic reagent & supply order | $23 | $- | $- | |
RNA Extraction Reagents | $10 | $10 | $10 |
Services | UF | Non-Profit | Commercial | Details |
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Additional sample plate | $25 | $25 | $25 | Price per additional sample plate run on the AB 7500 RT-PCR self-service instrument. |
Sample plate | $25 | $25 | $25 | Price per inital sample plate run on the AB 7500 RT-PCR self-service instrument. |
Training | $60 | $60 | $60 | Pricing is per one hour session, without a sample plate run. Or per three hour session with customer providing a sample plate to run on the instrument. |
Services | UF | Non-Profit | Commercial | Details |
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ds cDNA Synthesis | $125 | $140 | $165 | Service includes ds cDNA synethsis starting with total RNA using Mint2 kit. |
GA-B02: PCR Reaction | $10 | $10 | $20 | Service includes primers purchase, protocol design, and preparation for PCR reaction. |
GA-B03: PCR Purification | $10 | $10 | $20 | Service includes PCR purification for products of PCR reaction using QIAquick PCR Purification Kit. |
GA-C03: Primer Synthesis | $8 | $8 | $8 | Per primer service includes oligonucleotide synthesis of the desired sequence. |
GA-C04: Primer Screen/primer | $150 | $150 | $300 | Per primer screen. |
GA-C05: Primer Optimization/primer | $150 | $150 | $300 | Per primer optimization. |
GA-G03: gDNA extraction (<20 samples) | $15 | $30 | $45 | Per sample service includes gDNA extraction with less than 20 samples. |
gDNA Extraction (>20 samples) | $10 | $15 | $20 | Per sample service includes gDNA extraction from different organisms and tissues with 20 samples or up. |
microbiome DNA extraction | $20 | $24 | $27 | |
Plasmid DNA Extraction | $15 | $30 | $45 | Service includes plasmid DNA extraction for bacterial plate. |
Reverse Transcription (>20 samples) | $30 | $37.50 | $37.50 | Per sample (20 or more) service includes synthesis of cDNA starting with total RNA. |
Reverse Transcription (<20 samples) | $40 | $50 | $50 | Per sample (up to 20) service includes synthesis of cDNA starting with total RNA. |
RNA Clean-up | $22 | $33 | $44 | Per sample service includes RNA clean up for gDNA contaminated RNA samples. |
RNA Extraction | $50 | $60 | $75 | Service includes RNA extraction from different organisms and tissues as requested. If customer provides the reagents, discount will be applied. |
TA Cloning | $35 | $70 | $95 | Service includes PCR product cloned to TA vector. |
Services | UF | Non-Profit | Commercial | Details |
---|---|---|---|---|
Shipping | $40 | $- | $- |
Services | UF | Non-Profit | Commercial | Details |
---|---|---|---|---|
10XGenomics Single Cell 3'/5' RNAseq Library Prep (per sample) | $1765.50 | $1985.06 | $2205.63 | |
10XGenomics Single Cell CNV library Prep (Per sample batch with 16 samples are required) | $1720 | $1935 | $2150 | |
10XGenomics Single Human/Mouse T cell V(D)J Library Prep (per sample ) | $1850 | $2081.25 | $2312.50 | |
10X Genomics Single Cell Chip Cost | $247 | $247 | $247 | A chip cost will be applied if you perform less than 4 samples for 10X Genomics Chromium study. |
Luna Cell Count | $10.00 | $11.25 | $12.50 |
Services | UF | Non-Profit | Commercial | Details |
---|---|---|---|---|
Absolute Quantification Data Analysis (Per Standard Curve) | $30 | $45 | $60 | Service includes the creation of a standard curve to quantify unknowns to the standard curve and extrapolate a value. |
Mid Service on CFX | $30 | $35 | $40 | Per plate service is performed on a plate that is ready to run and includes data delivery. |
NextGen Sequencing Library Quantification | $45 | $50 | $67.50 | Service includes quantification of the NextGen Sequencing Library. |
NextGen Sequencing Library Quantification (30 or more submitted at one time) | $35 | $38.50 | $52.50 | Service includes quantification of the NextGen Sequencing Library with bulk rate discount. |
Plate Set-Up Fee for 384 well Absolute Quantification | $50 | $75 | $100 | Service includes 384 well plate set-up and cost of consumables. |
Plate Set-Up Fee for Pathway Plates (96 or 384 well) | $50 | $75 | $100 | Service includes 96 or 384 well plate set-up and cost of consumables. |
Plate/Opti-cap per plate | $10 | $15 | $20 | Per plate service includes the plate and Opti-cap. |
Post Amplification Read (SNP Genotyping) | $10 | $15 | $20 | Service is performed on a plate that is ready to run. |
Primer and Probe Sets Assay Design (20 reactions) | $30 | $35 | $40 | Technician uses software for primer design. |
qPCR Equipment Use Fee (Full Service) | $50 | $75 | $100 | Service is performed on a plate that is ready to run. |
qPCR Equipment Use Fee (Full Service) more than 5 plates per month. | $40 | $60 | $80 | Service is performed on a plate that is ready to run. |
RT Profiler PCR Arrays - Chemistry | $150 | $187.50 | $187.50 | Service includes expression analysis of a focused panel of genes. |
SYBR Green Chemistry qPCR Optimization | $1250 | $1875 | $2500 | Service includes the optimization of template and primers concentrations for better qPCR results. |
SYBR Green Dissociation Curve Test | $20 | $30 | $40 | Service is performed on a plate that is ready to run. |
SYBR Green qPCR (per sample/per gene) | $15 | $22 | $30 | Service includes SYBR Green qPCR analysis for one sample per gene done in triplicate. |
SYBR Green qPCR Reaction | $5 | $7.50 | $10 | Service includes SYBR Green qPCR analysis for one sample in one well starting with gDNA or cDNA. |
SYBR Green qPCR Reaction Mix | $100 | $150 | $200 | Service includes SYBR Green qPCR analysis per plate. |
SYBR Green RT-qPCR (per sample/per gene) | $25 | $38 | $50 | Service includes SYBR Green qPCR analysis for one sample per gene, done in triplicate, starting with total RNA. |
TaqMan Chemistry qPCR Optimization | $950 | $1425 | $1900 | Service includes optimization of template, primers and probe concentrations for better TaqMan assay result. |
TaqMan Low Density Array (TLDA) Chemistry and Plate Set-up | $160 | $240 | $320 | Service includes set-up and run of TaqMan Low Density Array and raw data delivery. |
TaqMan qPCR (1 reaction) | $7 | $10.50 | $14 | Service includes TaqMan qPCR analysis for one sample, in one well, starting with gDNA or cDNA. |
TaqMan qPCR (per sample/per gene) | $20 | $30 | $40 | Service includes TaqMan qPCR analysis for one sample per gene, done in triplicate. |
TaqMan qPCR reaction mix (per plate) | $150 | $225 | $300 | Service includes TaqMan qPCR analysis per plate. |
TaqMan RT-qPCR (per sample/per gene) | $30 | $45 | $60 | Service includes TaqMan qPCR analysis for one sample per gene, done in triplicate, starting with total RNA. |