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The service includes cloning and sequencing mouse heavy and light chains starting with total RNA, alignments of the heavy and light chains, to a detailed project report with annotated antibody DNA, protein sequences and CDRs.
Bravo 16s and RNAseq Library Automation (Bravo Automations)
16s ribosomal RNA sequencing can be used to identify and compare bacteria present within a given sample.
RNA-Seq is a powerful research tool for expression and variant analysis. Its’ application includes quantifying mRNA abundance; determining the transcriptional structure of genes: start sites, 5′ and 3′ ends, and splicing patterns; comparably detecting the changing expression levels of each transcript during development and under different conditions. It picks up all sequences that are present in the cell, regardless of their annotation status.
Gene Expression Core offers library preparation automation by Agilent Bravo Automation Workstation.The constructed libraries can be run on all NextGen sequencing platforms: Illumina HiSeq, MiSeq, and NextSeq. Sequence can be done by ICBR NextGen Sequencing Core.
We also offer Agilent’s SureSelect Target Enrichment platform which allows you to focus your NextGen sequencing workflow on key genomic regions of interest while reducing cost per sample. Other NextGen relative services include cDNA library construction, normalization and rRNA depletion (Ribominus). If requested, we will directly deliver the libraries to our ICBR NextGen Sequencing Core with an e-mail notification to you. Otherwise the library will be returned to you.
Custom Bravo Run (Bravo Automations)
The service includes the Bravo consumables and run assistance.
For NEW customers and NEW projects: Schedule a consultation to discuss experimental design and prepare a project outline with an ICBR staff member.
This service allows the customer to do absolute quantification, rare allele detection, quantification using TaqMan SNP array and CNV (Copy Number Variation) in a more sensitive, specific, and precise way.
Agilent 2100 Bioanalyzer/2200 TapeStation Analysis (RNA DNA QC)
**Attention: If you are submitting libaries for a sequencing run in the NextGen core, please request their library evaluation services**
The Agilent 2100 Bioanalyzer and 2200 TapeStation system automatically perform the multiple steps of gel-based electrophoresis, replacing the need to run agarose gels to separate and quantitate your RNA or DNA samples. A software tool, RNA Integrity Number (RIN) can help scientists estimate the integrity of total RNA samples. Data is returned in the form of PDF.
Sample Concentration Measurement (RNA DNA QC)
Quantitate micro-volume of samples via NanoDrop, or Qubit. Please provide the DNA size if caculated nM concentration is needed.
RNA-Seq is a powerful research tool for expression and variant analysis. Its’ application includes quantifying mRNA abundance; determining the transcriptional structure of genes; start sits, 5′ and 3′ ends, and splicing patterns; comparably detecting the changing expression levels of each transcript during development and under different conditions. It picks up all sequences that are present in the cell, regardless of their annotation status.
Service includes gDNA/RNA extraction, RNA clean up, PCR purification, revers transcription, ds cDNA synthesis, TA cloning and more
The 10x Genomics Chromium Controller System
The 10X Genomics Chromium Controller instrument is designed to capture thousands of single cells or DNA fragment, and combined them with reagents and gel bead (which contains barcoded oligonucleotides) in emulsion droplets (GEMs) to perform a variety of assays. Each GEM are uniquely barcoded.
It can perform Single Cell RNA-seq, Single Cell Copy Number Variation Profiling, Single Cell Immune Repertoire Profiling, Single Nuclei Expression Profiling, and Single Cell CITE-seq. For those assays, it can capture 500-10,000 individual cells per sample. Additionally, it partitions high molecular weight (HMW) DNA into individual GEMs to capture long-range information using Illumina sequencer. This assay requires low input of high molecular weight DNA to produce “linked read” information, which can be used for assembling genomes, assessing structural variants and obtaining genome phase information.
General Service (qPCR)
qPCR analysis offered at Gene Expression and Genotyping are: SYBR® or TaqMan™ reagents allowing absolute, relative, relative standard or comparative Ct quantification. Services includes but not limited to qPCR Absolute Quantification Data Analysis/Per Standard Curve; RT Profiler PCR arrays – chemistry; qPCR post amplification read (SNP); qPCR Mid service on CFX (1 plate); qPCR Plate set-up for 384 well Absolute Quantitation; qPCR primer and probe sets assay design.
Quantification of NextGen Sequencing libraries by qPCR
This method is widely used because its ability to target adapter sequences for a specific sequences in NextGen libraries. It enables accurate quantification of NextGen libraries. It measures the functional molecular of the libraries.
SYBR Green-based Detection (qPCR)
This real-time PCR assay uses SYBR Green Chemistry to detect PCR products as it accumulate during real time PCR process.
TaqMan-based Detection (qPCR)
This real-time PCR assay consists a target-specific probe and two gene specific primes that can increase the specificity of quantification PCR. The fluorescence single generated by probe allows quantitate measurements of the accumulated PCR product and increases the specificity of gene detection.
| Service/Request Type | Instrument | Core | Equipment | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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UF | ICBR Bioinformatics | Customized Data Analysis, Genome/Transcriptome Assembly and Annotation, Metagenomics Analysis, Quantitative DNA Sequence Mapping, Sequence Variation Analysis, Single Cell Analysis, Transcriptome Analysis, Consultation, Training | Bioinformatics | Visit Bioinformatics: Home | Staff & Contact | Services | Core Resources | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Customized Data Analysis | Customized Data Analysis | Bioinformatics | Contact UF | ICBR Bioinformatics for Customized Data Analysis: ICBR-Bioinformatics@ad.ufl.edu /// 352.273.8049 | No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Data Analysis Services | Customized Data Analysis | Bioinformatics | General category for bioinformatics work, including development of methods and software tools. Ad-hoc analysis services includes ad-hoc analysis, development of software tools and pipelines.UF | ICBR Bioinformatics /// ICBR-Bioinformatics@ad.ufl.edu /// 352.273.8049
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Back to top of page | Consultation, Training, Customized Data Analysis, Genome/Transcriptome Assembly and Annotation, Metagenomics Analysis, Quantitative DNA Sequence Mapping, Sequence Variation Analysis, Single Cell Analysis, Transcriptome Analysis, | Bioinformatics | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
UF | ICBR Cytometry | Analysis Workstations, Consumables, Cytometers, Histology, In-Vivo Imaging, Microscopes, Courses, Training, Software | Win 7, Win XP, Imaging Workstation, Accuri C6, Aria II, Canto II, Fortessa, LSR, NS300, Sony SH800 Cell Sorter, Sony Spectral SP6800, Cryostat - Microm HM505E, Microtome - Microm HM325, Leica VT1000S Vibratome, Bioluminescent - PerkinElmer IVIS Spectrum, Ultrasound - VisualSonics Vevo770, Keyence BZ-X800 Microscope, Leica DM2500, Leica TCS-SP5, Nikon A1RMP, Nikon Ti-E, Olympus IX70, Olympus IX81-DSU, Zeiss Axioplan 2, Cytoflex LX, BD FACSymphony A3 | Cytometry | Visit Cytometry: Home | Contact | Services | Instruments | Core Resources | Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Analysis Workstations | Analysis Workstations | Win 7, Win XP, Imaging Workstation, | Cytometry | Contact UF | ICBR Cytometry for Analysis Workstations: ICBR-Cytometry@ad.ufl.edu /// 352.273.8186 | Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Analysis Workstation - Win 7 | Analysis Workstations | Win 7 | Cytometry | Windows 7 based PC workstation for offline processing of data acquired on other CTAC imaging systems. This workstation has the following software installed:
Location: CTAC Imaging Suite (MBI LG-164) UF | ICBR Cytometry /// ICBR-Cytometry@ad.ufl.edu /// 352.273.8186
| Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Analysis Workstation - Win XP | Analysis Workstations | Win XP | Cytometry | Windows XP based PC workstation for offline processing of data acquired on other CTAC imaging systems. This workstation has the following software installed:
Location: CTAC Imaging Suite (MBI LG-164) UF | ICBR Cytometry /// ICBR-Cytometry@ad.ufl.edu /// 352.273.8186
| Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Imaging Workstation | Analysis Workstations | Imaging Workstation | Cytometry | Imaging workstation for the post-analysis of microscopic images. Location: CGRC 292 UF | ICBR Cytometry /// ICBR-Cytometry@ad.ufl.edu /// 352.273.8186No charge for use of this resource | Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Consumables | Consumables | Cytometry | Contact UF | ICBR Cytometry for Consumables: ICBR-Cytometry@ad.ufl.edu /// 352.273.8186 | Yes | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 70/100 Micron Sort Chip | Cytometers | Accuri C6 | Cytometry | 70/100 Micron Sort Chip.UF | ICBR Cytometry /// ICBR-Cytometry@ad.ufl.edu /// 352.273.8186
| Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Cytometers | Cytometers, | Accuri C6, Aria II, Canto II, Fortessa, LSR, NS300, Sony SH800 Cell Sorter, Sony Spectral SP6800, BD FACSymphony A3, Cytoflex LX | Cytometry | Contact UF | ICBR Cytometry for Cytometers: ICBR-Cytometry@ad.ufl.edu /// 352.273.8186 | Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Accuri C6 | Cytometers | Accuri C6 | Cytometry | This instrument is for analysis only and has 2 lasers (blue and red) with 4 fluorescence detectors (green, yellow-orange, red from blue laser, and red from red laser). Unlike most flow cytometers, there are no PMT voltages to adjust, making it a very easy system to learn and use. Location: CGRC 292 UF | ICBR Cytometry /// ICBR-Cytometry@ad.ufl.edu /// 352.273.8186
| Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Aria II | Cytometers | Aria II | Cytometry | CGRC Location (Self-Service at MBI): This instrument is for sorting only and has 5 lasers (UV, violet, blue, yellow-green, and red) with 15 fluorescence detectors (1 from UV laser, 3 from violet laser, 2 from blue laser, 5 from yellow-green laser, 3 from red laser, and is custom configurable by staff). This instrument uses FACS Diva (Windows XP). MBI Location (Self-Service): This instrument is for sorting only and has 5 lasers (UV, violet, blue, yellow-green, and red) with 15 fluorescence detectors (1 from UV laser, 3 from violet laser, 2 from blue laser, 5 from yellow-green laser, 3 from red laser, and is custom configurable by staff). This instrument uses FACS Diva (Windows XP). Locations: CGRC 292, MBI UF | ICBR Cytometry /// ICBR-Cytometry@ad.ufl.edu /// 352.273.8186
| Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| BD FACSymphony A3 | Cytometers | BD FACSymphony A3 | Cytometry | The BD FACSymphony A3 has 5 lasers, UV, Violet, Blue, Yellow/Green and Red. It is capable of detecting 28 colors with a HTS 96 or 384 well plate loader, so it can aspirate at high speed. samples from a plate or single 5 mls FACS tubes. This advanced instrument feature ultra-quiet VPX electronics system that supports up to 30 high-performance detectors and improves sensitivity to enable you to identify and analyze rare cell types and events. The capabilities of this platform technology uniquely allow you to conduct deep and broad phenotyping and gain richer scientific insights by fully leveraging the broad portfolio of BD Horizon Brilliant™ Reagents. Locations: MBI UF | ICBR Cytometry /// ICBR-Cytometry@ad.ufl.edu /// 352.273.8186
| Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Cytoflex LX | Cytometers | Cytoflex LX | Cytometry | The Cytoflex LX has 6 lasers, Blue, Red, Yellow/Green, Violet UV, and IR. It is capable of detecting 21 colors and has a 96 well plate loader so it can aspirate the samples from single 5 mls FACS tube or a plate. It has exquisite sensitivity and it is enabled for nano-particle analysis of Extracellular vesicles, viruses and small particles down to 80 nm sensitivity. This platform has extensive repositionable band pass filters and Avalanche Photodiode Detectors instead of PMTs for increased sensitivity. Location: CGRC UF | ICBR Cytometry /// ICBR-Cytometry@ad.ufl.edu /// 352.273.8186
| Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Fortessa | Cytometers | Fortessa | Cytometry | This instrument is for analysis only and has 4 lasers (blue, red, violet, and yellow-green) with 17 fluorescence detectors (3 on the blue laser, 3 on the red, 6 on the violet, 5 on the yellow-green, and is custom configurable by staff). This instrument uses FACS Diva (Windows 7). Location: CGRC 292 UF | ICBR Cytometry /// ICBR-Cytometry@ad.ufl.edu /// 352.273.8186
| Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| NS300 | Cytometers | NS300 | Cytometry | The NS300 is a nanoparticle counter and sizer. It illuminates the particles with a 488nm laser. A video camera records light scattering from the particles, and the software generates size profiles through nanoparticle tracking analysis, based on their Brownian motion. Fluorescence discrimination of particles is also possible. Samples containing nanoparticles in aqueous solution in the size range of 30-1000 nanometers can be measured. Location: CGRC 292 UF | ICBR Cytometry /// ICBR-Cytometry@ad.ufl.edu /// 352.273.8186
| Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Sony SH800 Cell Sorter | Cytometers | Sony SH800 Cell Sorter | Cytometry | This instrument is equipped with 3 lasers and 6 fluorescence detectors. It is capable of exciting fluorescent dyes in the violet, blue and red regions of the spectrum. Emission options run from the blue to the far red. (New users should first discuss their plans with a staff member to get feasibility details.) It can sort cells at rates of up to 10,000 per second, and offers 2-way sorting into tubes. This instrument is available for training and subsequent self-service use. Location: CGRC 292 UF | ICBR Cytometry /// ICBR-Cytometry@ad.ufl.edu /// 352.273.8186
| Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Sony Spectral SP6800 | Cytometers | Sony Spectral SP6800 | Cytometry | The Sony SP6800 Spectral Analyzer uses spectral technology to optimize sensitivity and enhance dim signal detection by collecting photons from 420nm to 800nm. Spectral technology also simplifies multicolor panel design, by eliminating bandpass filters and conventional compensation matrices while delivering better data and simplifying visualization for the study of heterogeneous populations. This system enhances dim signal detection for better visualization of rare populations, fluorescent proteins, and fluorochromes excited by multiple lasers. Spectral flow cytometry streamlines workflow and yields better data by keeping all the light collected. In conventional systems, overlapping fluorescence is subtracted using color compensation, so less light is collected. Instead, spectral flow cytometers sum the fluorescence together and then use unmixing to mathematically separate the colors. A unique prism collection system delivers emitted light to a 32 channel PMT. This produces 66 data points of signal detection for fluorescence and bright auto fluorescence to achieve accurate visualizations of fluorescent populations. This lets researchers see the complete spectral fingerprint of each fluorochrome from 420nm to 800nm. Location: CGRC 292 UF | ICBR Cytometry /// ICBR-Cytometry@ad.ufl.edu /// 352.273.8186
| Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Histology | Histology | Cryostat - Microm HM505E, Microtome - Microm HM325, Leica VT1000S Vibratome, | Cytometry | Contact UF | ICBR Cytometry for Histology: ICBR-Cytometry@ad.ufl.edu /// 352.273.8186 | Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Cryostat - Microm HM505E | Histology | Cryostat - Microm HM505E | Cytometry | Microm HM505E cryostat for cutting OCT frozen tissue sections. Users must provide their own supplies (including blades, OCT, slides, cover-slips, and tissue chucks (AKA specimen discs)).
Self Service Only - Training or Staff Assisted Operation is not available for this instrument. UF | ICBR Cytometry /// ICBR-Cytometry@ad.ufl.edu /// 352.273.8186
| Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Microtome - Microm HM325 | Histology | Microtome - Microm HM325 | Cytometry | Microm HM325 microtome for cutting paraffin embedded tissue sections. Users must provide their own supplies (including blades, slides, and cover-slips). For paraffin tissue processing & embedding services, see the Molecular Pathology Core at: https://molecular.pathology.ufl.edu/
UF | ICBR Cytometry /// ICBR-Cytometry@ad.ufl.edu /// 352.273.8186
| Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Leica VT1000S Vibratome | Histology | Leica VT1000S Vibratome | Cytometry | Leica VT1000S Vibratome for cutting fresh tissue sections. Users must provide their own supplies (including blades, cyanoacrylate glue (super-glue), slides, and cover-slips). For best results, sapphire blades are highly recommended. Location: CTAC Histology Resource Lab (MBI L3-192) UF | ICBR Cytometry /// ICBR-Cytometry@ad.ufl.edu /// 352.273.8186
| Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| In-Vivo Imaging | In-Vivo Imaging | Bioluminescent - PerkinElmer IVIS Spectrum, Ultrasound - VisualSonics Vevo770, | Cytometry | Contact UF | ICBR Cytometry for In-Vivo Imaging: ICBR-Cytometry@ad.ufl.edu /// 352.273.8186 | Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Bioluminescent - PerkinElmer IVIS Spectrum | In-Vivo Imaging | Bioluminescent - PerkinElmer IVIS Spectrum | Cytometry | PerkinElmer IVIS Spectrum Bioluminescent Imager Bioluminescent Imager for measuring and quantifying fluorescent or chemiluminescent (Luciferin/Luciferase) signal expression in in-vivo, ex-vivo, or in-vitro specimens. Software: PerkinElmerliving Image v4.5.5 on a Windows 7 workstation.
When performing in-vivo experiments using fluorescence, it is highly advisable to use a fluorescent label that emits above 600nm. The IVIS system includes an isoflurane vaporizer unit for in-vivo experiments, but users must provide their own isoflurane and charcoal scrubbers (2 charcoal canisters are required). Medical grade oxygen for running the vaporizer is provided with the system. Users must provide any other necessary reagents/supplies for prepping their samples.
Location: CTAC Imaging Suite (MBI LG-164) UF | ICBR Cytometry /// ICBR-Cytometry@ad.ufl.edu /// 352.273.8186
| Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Microscopes | Microscopes | Keyence BZ-X800 Microscope, Leica DM2500, Leica TCS-SP5, Nikon A1RMP, Nikon Ti-E, Olympus IX70, Olympus IX81-DSU, Zeiss Axioplan 2 | Cytometry | Contact UF | ICBR Cytometry for Microscopes: ICBR-Cytometry@ad.ufl.edu /// 352.273.8186 | Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Keyence BZ-X800 Microscope | Microscopes | Keyence BZ-X800 Microscope | Cytometry | The BZ-X800 Series Microscope is an inverted, fully-motorized microscope capable of performing high-resolution imaging (fluorescence, brightfield, phase-contrast), live cell analysis, well plate and multi-slide screening, and cell quantification with a single platform. With an intuitive, easy-to-use workflow for ease of use. Features:
UF | ICBR Cytometry /// ICBR-Cytometry@ad.ufl.edu /// 352.273.8186
| Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Leica DM2500 | Microscopes | Leica DM2500 | Cytometry | Leica DM2500 Upright Widefield Epifluorescent Microscope Basic manually controlled upright microscope for fluorescent or brightfield samples mounted on slides. Mercury HBO white light lamp for fluorescence excitation and DAPI, GFP, and Texas Red filter cubes. The system also has a Green/Red (dual band) filter cube for simultaneous two channel visual observation. Additional CFP, YFP, and Cy5 filter cubes are available for this microscope if needed. Objectives: 1.25x dry, 5x dry, 10x dry, 20x dry, 40x dry, 63x water. The microscope is equipped with a QImaging Retiga 4000R monochrome camera for fluorescent pseudo-color or brightfield monochrome imaging. This microscope is not equipped for imaging color brightfield stains. Images are acquired using QCapture Pro 7 software. UF | ICBR Cytometry /// ICBR-Cytometry@ad.ufl.edu /// 352.273.8186
| Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Leica TCS-SP5 | Microscopes | Leica TCS-SP5 | Cytometry | Confocal Microscope; objectives: 10X dry, 20X dry, 40X oil, 63X oil, 100X oil. This instrument has violet (also used for UV), indigo, blue, blue-green, green, and red lasers with 3 fluorescence channels which are totally configurable for any band. The environmental control is 37°C chamber, humidified, 5% CO2 capable. Stage: X-Y-Z computer control; tiling and 3D reconstruction available with FRET and FRAP wizards and is time-lapse capable. It uses LAS AF (Windows XP). UF | ICBR Cytometry /// ICBR-Cytometry@ad.ufl.edu /// 352.273.8186
| Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Nikon A1RMP | Microscopes | Nikon A1RMP | Cytometry | Nikon A1RMP Confocal/Multiphoton/STORM Microscope Laser-scanning confocal (LSCM), multiphoton (MP), and STORM (super-resolution) inverted microscope for fluorescent samples. Available Tokai-Hit stage top incubator with active CO2 and temperature regulation, and passive humidity, for imaging live cells in a 35mm dish or a chambered coverglass. Motorized XY stage movement for multi-point time-lapse imaging or tile-mapping (AKA mosaic or stitching). Galvo and resonant scanning. Software: Nikon NIS-Elements v5.0 on a Windows 7 workstation. (This software is also available (with the optional Deconvolution module) on our separate Windows 7 Analysis Workstation.)
Location: CTAC Imaging Suite (MBI LG-164) UF | ICBR Cytometry /// ICBR-Cytometry@ad.ufl.edu /// 352.273.8186
| Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Nikon Ti-E | Microscopes | Nikon Ti-E | Cytometry | Nikon Ti-E “Live Cell” Widefield Microscope (Motorized) Inverted widefield microscope with motorized XY stage for fluorescent or brightfield samples. Available Tokai-Hit stage top incubator with active CO2 and temperature regulation, and passive humidity, for imaging live cells in a 35mm dish or a chambered coverglass. Motorized XY stage movement for multi-point time-lapse or tile-mapping (AKA mosaic or stitching). Objectives: 10x dry, 20x dry, 40x dry, and 60x water. Multi-line LED lamp for fluorescent excitation. Fluorescent filter sets: DAPI, GFP/FITC, TRITC, Texas Red, and CY5. Phase Contrast optics for 10x and 20x. DIC (Nomarski) optics for 40x and 60x. Two cameras: Andor Zyla sCMOS 11 bit monochrome for fluorescence, and a Nikon DS-Fi2 color camera for stained brightfield samples. Software: Nikon NIS-Elements v4.51 on a Windows 7 workstation. (This software is also available (with the optional Deconvolution module) on our separate Windows 7 Analysis Workstation.
Location: CTAC Imaging Suite (MBI LG-164) UF | ICBR Cytometry /// ICBR-Cytometry@ad.ufl.edu /// 352.273.8186
| Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Olympus IX70 | Microscopes | Olympus IX70 | Cytometry | Olympus IX70 Inverted Widefield Epifluorescent Microscope Basic manually controlled inverted microscope for fluorescent or brightfield samples mounted on slides, or in dishes/plates. Mercury metal-halide white light lamp for fluorescence excitation and DAPI, GFP, TRITC filter cubes. An additional Cy5 filter cube may be installed if needed. Objectives: 4x dry, 10x dry, 20x dry LWD, 40x dry, 60x water. The microscope is equipped with a QImaging Retiga 4000R monochrome camera for fluorescent pseudo-color or brightfield monochrome imaging. This system also has an optional LCD color module which allows it to capture images of color stained samples in brightfield. Images are acquired using QCapture Pro 7 software. Location: CTAC Imaging Suite (MBI LG-164) UF | ICBR Cytometry /// ICBR-Cytometry@ad.ufl.edu /// 352.273.8186
| Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Olympus IX81-DSU | Microscopes | Olympus IX81-DSU | Cytometry | Olympus IX81-DSU Spinning Disk Confocal Microscope #1 (Manual Stage) Spinning disk confocal inverted microscope with manual XY stage for fluorescence labeled samples. Motorized Z-drive for basic confocal Z-stack imaging. Objectives: 10x dry, 20x dry, 40x dry, and 60x water. LED lamp for fluorescent excitation. Fluorescent filter sets: DAPI, GFP/FITC, TRITC, Texas Red, and CY5. Camera: Hamamatsu ORCA Flash 4.0 LT+ (16 bit monochrome). Software: Olympus CellSens
Spinning disk confocal inverted microscope with motorized XY stage for fluorescence labeled samples. Available Tokai-Hit stage top incubator with active CO2 and temperature regulation, and passive humidity, for imaging live cells in a 35mm dish. Motorized XY stage movement for tile-mapping (AKA mosaic or stitching) and Z-drive for basic confocal Z-stack imaging. Objectives: 10x dry, 20x dry, 20x dry LWD, 40x dry, and 60x water. LED lamp for fluorescent excitation. Fluorescent filter sets: DAPI, GFP/FITC, TRITC, Texas Red, and CY5. Camera: Hamamatsu ORCA Flash 4.0 LT+ (16 bit monochrome). Software: Olympus CellSens Locations: CGRC 292, MBI LG-164 UF | ICBR Cytometry /// ICBR-Cytometry@ad.ufl.edu /// 352.273.8186
| Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Courses, Analysis and Cell Staining | Training, Courses, Data Analysis, Cell Staining | Cytometry | Contact UF | ICBR Cytometry for Training, Cell Staining, Data Analysis and Workshops: ICBR-Cytometry@ad.ufl.edu /// 352.273.8186 | No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Flow Cytometry Course I/II | Courses, Training | Cytometry | Flow Cytometry Course I/II The focus of this course is to teach the best practices when using this cutting edge technology to produce the most reproducible publication quality data for your research studies. We will cover the basics of instrument operations to proper controls, experimental set up and design as well as hands-on techniques in proper instrument operations, as well as the acquisition of a stained 6 color PBMC panel. Following the acquisition of real samples we will practice proper compensation set up as well as manual vs. digital compensation in your experiments. All of these topics and skills are required for new as well as experienced users to learn correctly and to apply them correctly to your daily research. Course Contents:
UF | ICBR Cytometry /// ICBR-Cytometry@ad.ufl.edu /// 352.273.8186
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| ARIA Sorter Operator Training Course | Courses, Training | Cytometry | ARIA Sorter Operator Training Course Cost per person. UF | ICBR Cytometry /// ICBR-Cytometry@ad.ufl.edu /// 352.273.8186
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| High Parameter Panel Design and optimization Course on the Symphony | Courses, Training | Cytometry | High Parameter Panel Design and optimization Course on the Symphony Cost per person. UF | ICBR Cytometry /// ICBR-Cytometry@ad.ufl.edu /// 352.273.8186
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| Cell Staining Fee Per Hour | Courses, Training, Cell Staining | Cytometry | Cell Staining. Fee Per HourUF | ICBR Cytometry /// ICBR-Cytometry@ad.ufl.edu /// 352.273.8186
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| Cytometry Data Analysis - Flowjo/FCS express | Courses, Training, Cell Staining | Cytometry | Cytometry Data Analysis - Flowjo/FCS express. Fee per hourUF | ICBR Cytometry /// ICBR-Cytometry@ad.ufl.edu /// 352.273.8186
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| Software | Software | Cytometry | Contact UF | ICBR Cytometry for Software: ICBR-Cytometry@ad.ufl.edu /// 352.273.8186 | No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Annual Cost FCS Express Software Individual User Seat | Software | Cytometry | Annual Cost FCS Express Software Individual User Seat FCS express Software License Key Annual Fee. Annual Cost FCS Express Software Individual User Seat UF | ICBR Cytometry /// ICBR-Cytometry@ad.ufl.edu /// 352.273.8186
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| Annual Cost FLOWJO Software Individual User Seat | Software | Cytometry | Annual Cost FLOWJO Software Individual User Seat Annual Cost FCS Express Software Individual User Seat UF | ICBR Cytometry /// ICBR-Cytometry@ad.ufl.edu /// 352.273.8186
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| Back to top of page | Analysis Workstations, Consumables, Cytometers, Histology, In-Vivo Imaging, Microscopes, Courses, Training, | Win 7, Win XP, Imaging Workstation, Accuri C6, Aria II, Canto II, Fortessa, LSR, NS300, Sony SH800 Cell Sorter, Sony Spectral SP6800, Cryostat - Microm HM505E, Microtome - Microm HM325, Leica VT1000S Vibratome, Bioluminescent - PerkinElmer IVIS Spectrum, Ultrasound - VisualSonics Vevo770, Keyence BZ-X800 Microscope, Leica DM2500, Leica TCS-SP5, Nikon A1RMP, Nikon Ti-E, Olympus IX70, Olympus IX81-DSU, Zeiss Axioplan 2 | Cytometry | Yes | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
UF | ICBR Electron Microscopy | Electron Microscopes, Light Microscopes, Microtome, Sample Processing, TEM, SEM, High Pressure Freeze, Instrument Charges, | FEI G2 F20 TEM 200kV, FEI Spirit TEM 120kV, Hitachi H-7000 TEM, Hitachi SU5000 Schottky Field-Emission Variable Pressure SEM, Confocal Zeiss LSM800 microscope, Leica VT1000S Vibratome, Brightfield/Epifluorescent microscope, CPDs: Bal-tec & Tousimis, Denton DeskV Sputter coater, Leica EM AFS2, Leica HPM100, Microwave Ovens: PelcoBioWave & PelcoBioWavePro, Vitrobot, Critical point dryer, Cryo-Grid Storage, HPM-100 High Pressure Freezer, Leica CM1950 Cryostat, Meiji Imaging Station, Meiji dissecting microscope, Nikon Optiphot-2, Olympus BH2, Sputter Coater Run, Ultramicrotome | Electron Microscopy, | Visit Electron Microscopy: Home | Contact | Services | Instruments | Core Resources | Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Electron Microscopes | Electron Microscopes, SEM, TEM | FEI G2 F20 TEM 200kV, FEI Spirit TEM 120kV, Hitachi H-7000 TEM, Hitachi SU5000 Schottky Field-Emission Variable Pressure SEM, | Electron Microscopy | Contact UF | ICBR Electron Microscopy for Electron Microscopes: ICBR-ElectronMicroscopy@ad.ufl.edu /// 352.273.1184 | Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| FEI G2 F20 TEM 200kV | Electron Microscopes, TEM | FEI G2 F20 TEM 200kV | Electron Microscopy | This Tecnai G2 F20 TWIN is a 200 kV / FEG Transmission Electron Microscope with a Gatan UltraScan 4000 (4k x4k) CCD camera. It is used to collect high-resolution Cryo-TEM single particle images and tilt image series for electron tomography. Locations: Microbiology and Cell Sciences (MCS) Room 1012 UF | ICBR Electron Microscopy /// ICBR-ElectronMicroscopy@ad.ufl.edu /// 352.392.1184
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| FEI Spirit TEM 120kV | Electron Microscopes, TEM | FEI Spirit TEM 120kV | Electron Microscopy | This Tecnai G2 Spirit TWIN is a 120 kV Transmission Electron Microscope is for imaging plastic sections and for screening samples by negative staining. It is equipped with an UltraScan 1000 (2k x 2k) CCD camera and an AMT side mount camera. Locations: Microbiology and Cell Sciences (MCS) Room 1012 UF | ICBR Electron Microscopy /// ICBR-ElectronMicroscopy@ad.ufl.edu /// 352.392.1184
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| Hitachi H-7000 TEM | Electron Microscopes, TEM | Hitachi H-7000 TEM | Electron Microscopy | Conventional transmission electron microscope from Hitachi. This microscope is operated at 100 kV and suited for routine TEM imaging of non-cryo samples. It is equipped with a Veleta (2 k X 2k) CCD side mount camera. Locations: Microbiology and Cell Sciences (MCS) Room 1012 UF | ICBR Electron Microscopy /// ICBR-ElectronMicroscopy@ad.ufl.edu /// 352.392.1184
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| Hitachi SU5000 Schottky Field-Emission Variable Pressure SEM | Electron Microscopes, SEM | Hitachi SU5000 Schottky Field-Emission Variable Pressure SEM | Electron Microscopy | FE-SEM allows for a simple transition between high vacuum and variable pressure mode. EM Wizard is a knowledge-based system for SEM imaging. Locations: CGRC 185 UF | ICBR Electron Microscopy /// ICBR-ElectronMicroscopy@ad.ufl.edu /// 352.392.1184
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| Light Microscopes | Light Microscopes | Confocal Zeiss LSM800 microscope, Olympus BH2, Meiji Imaging Station, Nikon Optiphot-2 | Electron Microscopy | Contact UF | ICBR Electron Microscopy for Light Microscopes: ICBR-ElectronMicroscopy@ad.ufl.edu /// 352.273.1184 | Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Confocal Zeiss LSM800 microscope | Light Microscopes | Confocal Zeiss LSM800 microscope | Electron Microscopy | Confocal laser scanning microscope (Axio Observer Z1) inverted microscope. Computer command center with Zen Blue System. Equipped with objectives lenses: 10x (high res, dry), 10x (wide field view, dry), 20x (dry), 40x (water) and 63x (oil). This instrument has four diode laser sources, 405, 488, 561 and 640 nm. Two multi-alkaline PMT detectors and one transmitted light detector (DIC).UF | ICBR Electron Microscopy /// ICBR-ElectronMicroscopy@ad.ufl.edu /// 352.392.1184
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| Microtome | Microtome | Leica CM1950 Cryostat, Leica VT1000S Vibratome, Ultramicrotome, | Electron Microscopy | Contact UF | ICBR Electron Microscopy for Microtome: ICBR-ElectronMicroscopy@ad.ufl.edu /// 352.273.1184 | Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Leica CM1950 Cryostat | Microtome | Leica CM1950 Cryo-Stat | Electron Microscopy | Rotary cryo-microtome enclosed in a temperature-controlled box (-35oC – 0oC). Frozen samples could be sliced into 5 to 100 micrometer sections. Typically used for histological sections. Equipped with UV chamber disinfection and vacuum-assisted waste removal.UF | ICBR Electron Microscopy /// ICBR-ElectronMicroscopy@ad.ufl.edu /// 352.392.1184
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| Leica VT1000S Vibratome | Microtome | Leica VT1000S Vibratome | Electron Microscopy | The Leica VT1000S Vibratome is a vibrating blade microtome used to produce 1 – 999um sections of fixed or fresh tissue under physiological conditions without freezing or embedding. Sectioning fresh tissue specimens with the VT1000S maintains the morphology, enzyme activity and cell viability of the tissue. The vibratome also minimizes artifacts, compression distortion, cell destruction and other inherent deleterious effects of sectioning.UF | ICBR Electron Microscopy /// ICBR-ElectronMicroscopy@ad.ufl.edu /// 352.392.1184
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| Ultramicrotome | Microtome | Ultramicrotome | Electron Microscopy | Ultramicrotome for slicing plastic-embedded samples into 60-500 nm thin sections. Ultracut R, Leica EM UC7, Leica Ultracut UCT, RMC PoweTomeX, HM 335 E UF | ICBR Electron Microscopy /// ICBR-ElectronMicroscopy@ad.ufl.edu /// 352.392.1184
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| Sample Processing | Sample Processing | CPDs: Bal-tec & Tousimis, Denton DeskV Sputter coater, Leica EM AFS2, Leica HPM100, Microwave Ovens: PelcoBioWave & PelcoBioWavePro, Vitrobot | Electron Microscopy | Contact UF | ICBR Electron Microscopy for Sample Processing: ICBR-ElectronMicroscopy@ad.ufl.edu /// 352.273.1184 | Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Vitrobot | Sample Processing | Vitrobot | Electron Microscopy | Automated plunge-freezer for cryoTEM. For reproducible vitrification (freezing without ice crystal formation), users can control temperature, relative humidity, blotting conditions and freezing velocity..UF | ICBR Electron Microscopy /// ICBR-ElectronMicroscopy@ad.ufl.edu /// 352.392.1184
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| Instrument Charges | Instrument Charges | Brightfield/Epifluorescent microscope, Confocal Zeiss LSM800 microscope, Hitachi SU5000 Schottky Field-Emission Variable Pressure SEM, HPM-100 High Pressure Freezer, Leica CM1950 Cryostat, Meiji dissecting microscope, TEM FEI F20, Pelco Microwave oven, Sputter Coater Run, TEM FEI Spirit, TEM Hitachi H-7000, Ultramicrotome, Vitrobot, Cryo-Grid Storage, Critical point dryer | Electron Microscopy | Contact UF | ICBR Electron Microscopy: ICBR-ElectronMicroscopy@ad.ufl.edu /// 352.273.1184 | No | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Critical Point Dryer | Instrument Charges, High Pressure Freezer | Critical point dryer | Electron Microscopy | Price per run of critical point dryer. CPD is an established method of dehydrating biological tissue prior to examination in a scanning electron microscope (SEM). Gently dries biological specimens such as pollen, tissue, plants, insects, etc. as well as industrial samples for MEMS.UF | ICBR Electron Microscopy /// ICBR-ElectronMicroscopy@ad.ufl.edu /// 352.392.1184
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| HPM-100 High Pressure Freezer | Instrument Charges, High Pressure Freezer | HPM-100 High Pressure Freezer | Electron Microscopy | Price per hr for Leica HPM-100 HPF used for cryofixation of biological and industrial samples. Ultrastructural and antigenicity preservation by high pressure freezing is superior to conventional chemical fixation. This HPM100 is a high pressure freezing system from Leica Microsystems for freezing of specimens up to 250 μm in thickness. Other charges may apply.UF | ICBR Electron Microscopy /// ICBR-ElectronMicroscopy@ad.ufl.edu /// 352.392.1184
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| Pelco Microwave oven | Instrument Charges, High Pressure Freezer | Pelco Microwave oven | Electron Microscopy | Daily fee for Pelco microwave processor. Microwave-assisted process rapidly fix and embed biological samples for EM. Microwave generated in the chamber along with gently vacuum accelerates penetration of fixatives and exchange of resin molecules significantly shortening sample processing time.UF | ICBR Electron Microscopy /// ICBR-ElectronMicroscopy@ad.ufl.edu /// 352.392.1184
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| Sputter Coater Run | Instrument Charges, High Pressure Freezer | Sputter Coater Run | Electron Microscopy | Price per run of the Desk V. Deposits gold palladium or carbon for rendering SEM samples conductive. Equipped with adjustable rotating and tilting stage with ultra-pure argon gas to ensure a highly uniform coating with excellent conformity. The Desk V converts easily from metal sputter to carbon evaporation.UF | ICBR Electron Microscopy /// ICBR-ElectronMicroscopy@ad.ufl.edu /// 352.392.1184
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| Back to top of page | Electron Microscopes, Light Microscopes, Microtome, Sample Processing, TEM, SEM, High Pressure Freeze, Instrument Charges, | Confocal Zeiss LSM800 microscope, Olympus BH2, Meiji Imaging Station, Nikon Optiphot-2, Leica CM1950 Cryostat, CPDs: Bal-tec & Tousimis, Denton DeskV Sputter coater, Leica EM AFS2, Leica HPM100, Microwave Ovens: PelcoBioWave & PelcoBioWavePro, Vitrobot, Brightfield/Epifluorescent microscope, Confocal Zeiss LSM800 microscope, Hitachi SU5000 Schottky Field-Emission Variable Pressure SEM, HPM-100 High Pressure Freezer, Leica VT1000S Vibratome, Meiji dissecting microscope, TEM FEI F20, Pelco Microwave oven, Sputter Coater Run, TEM FEI Spirit, TEM Hitachi H-7000, Ultramicrotome, Cryo-Grid Storage | Electron Microscopy | Yes | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
UF | ICBR Gene Expression & Genotyping | Agilent, Antibody Sequencing, Bravo Automation, PCR, Sample Prep, Sample Preparation, CRISPR, Data Analysis, Digital PCR Analysis, ELISA, RNA DNA QC, RNASeq & NGS Related Services, Real-Time PCR, Single Cell Analysis, qPCR, 10x Genomics, General Services, Consultation, Reagents, Supplies, GeneTitan Axiom Array, NextGen Library Automation | Gene Expression & Genotyping, | Visit Gene Expression & Genotyping: | No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Agilent | Agilent | Gene Expression & Genotyping | Contact UF | ICBR Gene Expression & Genotyping for Agilent Services: ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043 | No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Agilent SureSelect Target Enrichment | Agilent | Gene Expression & Genotyping | Agilent SureSelect Target Enrichment.UF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
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| Antibody Sequencing | Antibody Sequencing | Gene Expression & Genotyping | Contact UF | ICBR Gene Expression & Genotyping for Antibody Sequencing: ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043 | No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Antibody Sequencing (VH VL domain) | Antibody Sequencing | Gene Expression & Genotyping | The service includes cloning and sequencing mouse heavy and light chains starting with total RNA, alignments of the heavy and light chains, to a detailed project report with annotated antibody DNA, protein sequences and CDRs. There will be extra $50 charge for RNA extraction if you submit hybridoma cell pellets (1X106). Sanger sequencing cost is not included. The cost will differ depending which outsource vendor will be used and how many clonies will be sequenced. UF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
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| Data Analysis | Data Analysis | Gene Expression & Genotyping | Contact UF | ICBR Gene Expression & Genotyping for Data Analysis: ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043 | No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Data Analysis | Data Analysis | Gene Expression & Genotyping | Service includes array analysis, p-value, FDR and fold change.UF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
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| Digital PCR Analysis | Digital PCR Analysis | Gene Expression & Genotyping | Contact UF | ICBR Gene Expression & Genotyping for Digital PCR Analysis: ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043 | No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Digital PCR Drop-off Service | Digital PCR Analysis | Gene Expression & Genotyping | Service includes starting with ready to run BioRad digital PCR plate to raw data file delivery. Process includes droplet generation, PCR amplification, read the plate reader and raw data file delivery. UF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
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| Digital PCR Droplet Auto Generation/Plate Reader | Digital PCR Analysis | Gene Expression & Genotyping | Service includes the 1st 8 rections for the drop off plate UF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
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| Digital PCR full service | Digital PCR Analysis | Gene Expression & Genotyping | Service includes the 1st 8 rections set up and date delivery as excel format for BioRad digital PCR analysis with primers/assays provided by customer. ICBR-GeneExpression@ad.ufl.edu | 352.273.8043
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| GeneTitan Axiom Array | GeneTitan Axiom Array | Gene Expression & Genotyping | Contact UF | ICBR Gene Expression & Genotyping for GeneTitan Axiom Array: ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043 | No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| GeneTitan Axiom Plate Processing | GeneTitan Axiom Array | GeneTitan | Gene Expression & Genotyping | GeneTitan Axiom Plate ProcessingUF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
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| NextGen Library Automation | NextGen Library Automation | Agilent Bravo Liquid Handling Platform | Gene Expression & Genotyping | Contact UF | ICBR Gene Expression & Genotyping for NextGen Library Automation: ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043 | No | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 16 s library Automation Per Sample | Bravo Automation, NextGen Library Automation | Agilent Bravo Liquid Handling Platform | Gene Expression & Genotyping | Bravo Automation.UF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
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| Primer transfer from 96-well to 384-well plate | Bravo Automation, NextGen Library Automation | Agilent Bravo Liquid Handling Platform | Gene Expression & Genotyping | Bravo Automation.UF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
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| Illumina mRNA-seq library Miniaturization with Mosquito | Bravo Automation, NextGen Library Automation | Bravo Automation, Mosquito | Gene Expression & Genotyping | Illumina mRNA-seq library Miniaturization with MosquitoUF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
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| Illumina Total RNA-seq library Miniaturization with Mosquito | RNASeq & NGS Related Services, NextGen Library Automation | Bravo Automation, Mosquito | Gene Expression & Genotyping | Illumina Total RNA-seq library Miniaturization with MosquitoUF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
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| RNA DNA QC | RNA DNA QC | Gene Expression & Genotyping | Contact UF | ICBR Gene Expression & Genotyping for RNA DNA QC Services: ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043 | No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Bioanalyzer run for DNA RNA QC (up to 11/12 samples) | RNA DNA QC | Gene Expression & Genotyping | Service includes RNA and DNA quality assessment, reagents, chip, and ladder for Nano, Pico, DNA 1000, or DNA 12000. Due to increased reagent cost, the Bioanalyzer/TapeStation service fees is increased accordingly starting Jan 2, 2018. Please check the service fee before you submit your RNA/DNA QC samples. UF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
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| DNA High Sensitivity Bioanalyzer (up to 11 samples) | RNA DNA QC | Gene Expression & Genotyping | Service includes fragmented DNA, NextGen libray quality assessment with concentrations as low as 5 pg/µL. 11samples/per chip. Due to increased reagent cost, the Bioanalyzer/TapeStation service fees is increased accordingly starting Jan 2, 2018. Please check the service fee before you submit your RNA/DNA QC samples. UF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
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| DNA High Sensitivity TapeStation per plate ( 96 samples) for DNA, Fragment, NextGen library QC | RNA DNA QC | Gene Expression & Genotyping | DNA High Sensitivity TapeStation per plate (96 samples) for DNA Fragment, NextGen library QC: High Sensitivity DNA ScreenTape per plate service for DNA fragment and NextGen libraries with the sample concentration of 10-1000pg/ul.UF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
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| DNA High Sensitivity TapeStation/Bioanalyzer run (per sample) for DNA Fragment, NextGen library QC | RNA DNA QC | Gene Expression & Genotyping | Service includes fragmented DNA, NextGen library quality assessment with concentrations as low as 5pg/ul. Due to increased reagent cost, the Bioanalyzer/TapeStation service fees is increased accordingly starting Jan 2, 2018. Please check the service fee before you submit your RNA/DNA QC samples. UF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
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| DNA RNA QC by TapeStation/Bioanalyzer (per sample) | RNA DNA QC | Gene Expression & Genotyping | Service includes RNA and DNA quality assessment, reagents, chip, and ladder for Nano, Pico, DNA 1000, or DNA 12000. Minimum of 7 samples to run chip. If submitting less than 7 samples, customer will have to wait until we have at least 7 samples to fill up the chip. Due to increased reagent cost, the Bioanalyzer/TapeStation service fees is increased accordingly starting Jan 2, 2018. Please check the service fee before you submit your RNA/DNA QC samples. UF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Small RNA Bioanalyzer | RNA DNA QC | Gene Expression & Genotyping | Service includes resolving small nucleic acids in the size range of 6 to 150 nt. 11 samples/ per chip. Due to increased reagent cost, the Bioanalyzer/TapeStation service fees is increased accordingly starting Jan 2, 2018. Please check the service fee before you submit your RNA/DNA QC samples. UF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| TapeStation per plate (96 samples) | RNA DNA QC | Gene Expression & Genotyping | TapeStation per plate (96 samples): DNA ScreenTape per plate service for DNA fragment and NextGen libraries with the sample concentration of 1-50ng/ul, or RNA ScreenTape per plate service with the sample concentration of 25-500ng/ul.UF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| RNA DNA QC-Concentration Measurement | RNA DNA QC | Gene Expression & Genotyping | Contact UF | ICBR Gene Expression & Genotyping for RNA DNA QC-Concentration Measurement: ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043 | No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Qubit Quantification (one charge for every 1-10 samples) | RNA DNA QC | Gene Expression & Genotyping | Service includes quantification of DNA and RNA samples by using Qubit. Cost of reagents included.UF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| RNASeq & NextGen Related Services | RNASeq & NGS Related Services | Gene Expression & Genotyping | Contact UF | ICBR Gene Expression & Genotyping for RNASeq and NextGen Related Services: ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043 | No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| COVID-seq Library Prep with Mosquito | RNASeq & NGS Related Services | Mosquito | Gene Expression & Genotyping | COVID-seq Library Prep with MosquitoUF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
| No | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Illumina RNA-Seq libraries (Poly A) | RNASeq & NGS Related Services | Gene Expression & Genotyping | Service includes total RNA QC with Bioanalyzer, library construction, library QC, and library delivery. There will be extra $50 if starts with cell pellet or tissue samples.UF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Illumina RNA-Seq library Prep for low input, ribo-depletion or degraded RNA | RNASeq & NGS Related Services | Gene Expression & Genotyping | Illumina RNA-Seq library Prep for low input, ribo-depletion or degraded RNA.UF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Illumina Small RNA libray w/Barcode | RNASeq & NGS Related Services | Gene Expression & Genotyping | Service includes total RNA QC with Bioanalyzer, library construction, library QC, and library delivery.UF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| rRNAs Depletion | RNASeq & NGS Related Services | Gene Expression & Genotyping | Service includes removal of human, mouse, rat, bacteria, and plant ribosomal RNA.UF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Sample Prep and Other Services | Sample Prep | Gene Expression & Genotyping | Contact UF | ICBR Gene Expression & Genotyping: ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043 | No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| ds cDNA Synthesis | Sample Preparation | Gene Expression & Genotyping | Service includes ds cDNA synethsis starting with total RNA using Mint2 kit.UF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 70 ml Reagent Trough with Lid | Sample Preparation | Gene Expression & Genotyping | 70 ml Reagent Trough with Lid (per piece as requested) UF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 170 ml Reagent Trough | Sample Preparation | Gene Expression & Genotyping | 170 ml Reagent Trough (per piece as requested) UF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| gDNA extraction | Sample Preparation | Gene Expression & Genotyping | Per sample service includes gDNA extraction from different organisms and tissuesUF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Microbiome DNA Extraction | Sample Preparation, Sample Prep | Gene Expression & Genotyping | Microbiome DNA Extraction.UF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| QiaCube Walk up Service | Sample Preparation | Qiacube | Gene Expression & Genotyping | QiaCube Walk up Service UF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
| No | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Reverse Transcription (>20 samples) | Sample Preparation | Gene Expression & Genotyping | Reverse Transcription (>20 samples)UF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| RNA Extraction | Sample Preparation, Sample Prep | Gene Expression & Genotyping | Service includes RNA extraction from different organisms and tissues as requested. If customer provides the reagents, discount will be applied.UF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Single Cell Analysis | Single Cell Analysis, 10x Genomics | Gene Expression & Genotyping | Contact UF | ICBR Gene Expression & Genotyping for Single Cell Analysis: ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043 | No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 10x Genomics Single Cell Library Prep | Single Cell Analysis, 10x Genomics | Gene Expression & Genotyping | UF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 10x Load on only | Single Cell Analysis, 10x Genomics | Gene Expression & Genotyping | Load on only UF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 10x Single Cell Chip Cost | Single Cell Analysis, 10x Genomics | Gene Expression & Genotyping | Reagent UF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Luna Cell Count | Single Cell Analysis, 10x Genomics | Gene Expression & Genotyping | Luna Cell CountUF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| qPCR | qPCR | Gene Expression & Genotyping | Contact UF | ICBR Gene Expression & Genotyping for qPCR Services: ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043 | No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Mid Service on CFX | qPCR | Gene Expression & Genotyping | Per plate service is performed on a plate that is ready to run and includes data delivery.UF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| NextGen Sequencing Library Quantification | qPCR | Gene Expression & Genotyping | Service includes quantification of the NextGen Sequencing Library.UF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| SYBR Green qPCR Reaction | qPCR | Gene Expression & Genotyping | SYBR Green qPCR Reaction.UF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| TaqMan qPCR (1 reaction) | qPCR | Gene Expression & Genotyping | TaqMan qPCR (1 reaction).UF | ICBR Gene Expression & Genotyping /// ICBR-GeneExpression@ad.ufl.edu /// 352.273.8043
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Back to top of page | Affymetrix, Agilent, Antibody Sequencing, Bravo Automation, Real-Time PCR, Sample Prep, Sample Preparation, CRISPR, Data Analysis, Digital PCR Analysis, ELISA, RNA DNA QC, RNASeq & NGS Related Services, Real-Time PCR, Single Cell Analysis, qPCR, 10x Genomics, General Services, Consultation, Reagents, Supplies, GeneTitan Axiom Array, NextGen Library Automation | Gene Expression & Genotyping | No | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
UF | ICBR Monoclonal Antibody | Biotinylation, Cloning, Consultation, Deposit, Direct Detect, ELISA, Fusion, Immunization, Microplate Readers, Octet, Octet Qke System, Octet Services, Plate Reader, Production, Protein Quantitation, Protein Simple WES, Purification, Shipping, Spleen, Storage, Subculture, Supplies, Reagents, Western, Workshops, Training, | Monoclonal Antibody | Visit Monoclonal Antibody: Home | Contact | Services | Instruments | Core Resources | Yes | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Biotinylation | Biotinylation | Monoclonal Antibody | Contact UF | ICBR Monoclonal Antibody for Biotinylation Services: ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040 | No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Biotinylation (custom project) | Biotinylation | Monoclonal Antibody | Biotinylation of purified antibodies that includes biotin incorporation assay. Standard scale up to 5 mgs.UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Cloning | Cloning | Monoclonal Antibody | Contact UF | ICBR Monoclonal Antibody for Cloning Services: ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040 | No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Cloning | Cloning | Monoclonal Antibody | Single cell cloning of uncloned hybridoma cell line from fusion. Service will include: deposit of two monoclonal cell lines (primary and secondary), isotyping of primary cell line, mycoplasma testing of primary cell line. The Monoclonal Antibody Core will screen supernatants from single cell wells by ELISA for an additional fee.UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Cloning (deposit 3 additional vials) | Cloning | Monoclonal Antibody | UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Cloning (freeze extra vials) | Cloning | Monoclonal Antibody | Freezing of additional vials of primary and/or secondary clone. Price per vial.UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Cloning (No positives) | Cloning | Monoclonal Antibody | sufficient outgrowth of single colony wells, no antibody positives.UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Deposit | Deposit | Monoclonal Antibody | Contact UF | ICBR Monoclonal Antibody for Deposit Services: ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040 | No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Deposit Hybridoma Line Produced Outside ICBR | Deposit | Monoclonal Antibody | This service includes producing 4 vials for deposit. The price does not include cryo-storage or mycoplasma testing.UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Isotyping | Deposit | Monoclonal Antibody | UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Mycoplasma Testing | Deposit | Monoclonal Antibody | Mycoplasma Testing using Lonza Mycoalert kit and controls. Samples #2-4 must run in parallel and on same day as Sample #1.UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| ELISA | ELISA | Monoclonal Antibody | Contact UF | ICBR Monoclonal Antibody for ELISA Services: ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040 | No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| ELISA Screening | ELISA | Monoclonal Antibody | 96 well plate format. Price per plate.UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Fusion | Fusion | Monoclonal Antibody | Contact UF | ICBR Monoclonal Antibody for Fusion Services: ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040 | No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Cell Fusion | Fusion | Monoclonal Antibody | UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Immunization | Immunization | Monoclonal Antibody | Contact UF | ICBR Monoclonal Antibody for Immunization Services: ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040 | No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| ACS Mouse Order Standard Fee | Immunization | Monoclonal Antibody | UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| CpG Adjuvant (10 ug) | Immunization | Monoclonal Antibody | Adjuvant CpG, per 10 ul.UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Animal Weekend VetTech Checks (Per 2 Cages) | Immunization | Monoclonal Antibody | UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Housing Mice | Immunization | Monoclonal Antibody | Monthly housing charge after the first 6 months. Price per cage.UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Immunization Fee Per Cage | Immunization | Monoclonal Antibody | Immunization Fee per cageUF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Mouse | Immunization | Monoclonal Antibody | UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| MPL + TDM Adjuvant 9 Immunizations 60 day Expiration | Immunization | Monoclonal Antibody | MPL + TDM Emulsion (Sigma)UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Ordering Fee | Immunization | Monoclonal Antibody | UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Serum Titer Bleed per Cage | Immunization | Monoclonal Antibody | UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Microplate Readers | Microplate Readers | Monoclonal Antibody | Contact UF | ICBR Monoclonal Antibody for Microplate Readers: ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040 | No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| BioTek Synergy H4 | Microplate Readers | Monoclonal Antibody | BioTek Synergy H4 Multimode Microplate Reader: Self service All-in-One Hybrid Microplate Reader. The Gen5 Data Analysis Software is compatible with Take3™ Multi-Volume Plate with 2 uL microspots for low volume assays; Tunable monochromator wavelength range 230 – 999 nm, 1 nm increments; Microplate types: 1- to 384-well plates. High-Performance Luminescence with Fluorescence Detectors: Fluorescence intensity (FI), Fluorescence Polarization (FP), Time- Resolved Fluorescence (TRF), and TR-FRET.UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Spectramax M5 Multimode | Microplate Readers | Monoclonal Antibody | Self service Soft max PRO 5.3 system. It is a five-mode multi-detection microplate reader system: Fluorescence Intensity, Absorbance, Luminescence, Time-Resolved Fluorescence (TRF) and Fluorescence Polarization (FP) assays. Turnable monochromator wavelength range is 200 - 1000 nm with 1 nm increments.UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Octet | Octet | Monoclonal Antibody | Contact UF | ICBR Monoclonal Antibody for Octet Services: ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040 | No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Octet QKe Full Service-charged per hour (2 hour minimum) | Octet | Monoclonal Antibody | Does not include sensors or reagents/supplies; time cannot be split and does not rollover to multiple days.UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Octet Quantitation | Octet | Monoclonal Antibody | IgG subclass; Includes sensors. Price per sample.UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Octet Services | Octet Services | Monoclonal Antibody | Contact UF | ICBR Monoclonal Antibody for Octet Services: ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040 | No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Octet OKe System Self-Service per hour | Octet Services | Monoclonal Antibody | This includes the Qke buffer and plate, gloves, and pipette tipsUF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Production | Production | Monoclonal Antibody | Contact UF | ICBR Monoclonal Antibody for Production Services: ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040 | No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| CL 350 | Production | Monoclonal Antibody | UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Monoclonal antibody production | Production | Monoclonal Antibody | Cell culture supernatantUF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Protein Quantitation | Protein Quantitation | Monoclonal Antibody | Contact UF | ICBR Monoclonal Antibody for Protein Quantitation: ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040 | No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Direct Detect Protein Quantitation | Protein Quantitation | Monoclonal Antibody | Analyze proteins using Millipore Direct Detect Infrared Spectrometer. Price per card.UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Purification | Purification | Monoclonal Antibody | Contact UF | ICBR Monoclonal Antibody for Purification Services: ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040 | No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Purification | Purification | Monoclonal Antibody | Tandem, Small Scale and Large ScaleUF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Shipping | Shipping | Monoclonal Antibody | Contact UF | ICBR Monoclonal Antibody: ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040 | No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Shipping | Shipping | Monoclonal Antibody | UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Spleen | Spleen | Monoclonal Antibody | Contact UF | ICBR Monoclonal Antibody for Spleen Services: ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040 | No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Spleen Harvest | Spleen | Monoclonal Antibody | Includes removing spleen, processing, counting, and cryopreserving.UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Storage | Storage | Monoclonal Antibody | Contact UF | ICBR Monoclonal Antibody: ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040 | No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Cryo-Storage Retrieval | Storage | Monoclonal Antibody | UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Cryostorage Yearly Fee | Storage | Monoclonal Antibody | To be paid yearly for storage UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Freeze Uncloned Hybridomas (<20 cell lines) | Storage | Monoclonal Antibody | Limit of 2 vials per cell line. Price per cell line.UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Store Uncloned Hybridomas (per month) | Storage | Monoclonal Antibody | Annual fee for storage of uncloned hybridoma cell lines. Price per cell line. Per monthUF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Subculture | Subculture | Monoclonal Antibody | Contact UF | ICBR Monoclonal Antibody for Subculture Services: ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040 | No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Freeze 2 Extra CryoVials Per Line | Subculture | Monoclonal Antibody | UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Subculture ICBR Produced Hybridoma Cells | Subculture | Monoclonal Antibody | Subculture of hybridoma cellsUF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Supplies & Reagents | Reagents, Supplies | Monoclonal Antibody | Contact UF | ICBR Monoclonal Antibody: ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040 | No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Kits, supplies, reagents (per unit) | Supplies, Reagents | Monoclonal Antibody | UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Ordering Fee | Supplies, | Monoclonal Antibody | UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Western | Western | Monoclonal Antibody | Contact UF | ICBR Monoclonal Antibody for Western Services: ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040 | Yes | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Traditional Western Blot | Western | Monoclonal Antibody | UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| Yes | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Wes Equipment Cost Per Run | Western | Monoclonal Antibody | UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| Yes | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Wes Equipment Full Service (per run) | Western | Monoclonal Antibody | Wes Equipment Full Service (per run)UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| Yes | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Workshops | Workshops, Training | Monoclonal Antibody | Contact UF | ICBR Monoclonal Antibody: ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040 | No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Introduction to Mammalian Cell Culture Techniques | Workshops, Training | Monoclonal Antibody | This two-day workshop is designed for those new to mammalian cell culture or needing a refresher course on the basics. The workshop includes informal lectures and hands-on experience. The major topics are: maintaining mammalian cells in culture (aseptic technique, thawing, counting, expanding, freezing) and troubleshooting (rescuing sick cells and recogizing and dealing with contamination).UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Consultations, Training & Assistance (per hour) | Workshops, Training, Consultations | Monoclonal Antibody | Consultations, Training & Assistance (per hour)UF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Sample Processing and Reagent Preparation | Workshops, Training, Consultations | Monoclonal Antibody | Sample Processing and Reagent PreparationUF | ICBR Monoclonal Antibody /// ICBR-MonoclonalAntibody@ad.ufl.edu /// 352.273.8040
| No | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Back to top of page | Biotinylation, Cloning, Consultation, Deposit, Direct Detect, ELISA, Fusion, Immunization, Microplate Readers, Octet, Octet Qke System, Octet Services, Plate Reader, Production, Protein Quantitation, Protein Simple WES, Purification, Shipping, Spleen, Storage, Subculture, Supplies, Reagents, Western, Workshops, Training | Monoclonal Antibody | Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
UF | ICBR NextGen DNA Sequencing | 10x Genomics Library Construction, Consultation, 10x Genomics, Covaris Fragmentation, Data Delivery Media, General Services: Load only run & pooling, Illumina Library Construction Services, Illumina Sequenicng Services, Library or Fragment Selection, Library or sample clean-up and quantitation, Miscellaneous Services, NovaSeq Services, PacBio Library Construction Services, Sample or Library Evaluation, Workshops, Training, iSeq Sequencing Service, PacBio SEQUEL IIe Sequencing Runs, Illumina MiSeq Sequencing, Illumina iSeq Sequencing, Library or Sample QC and Clean-up, Illumina NovaSeq6000 Sequencing, Library or Fragment Size Selection and Pooling Illumina MiSeq Sequencing, Illumina iSeq Sequencing, Library or Sample QC and Clean-up, Illumina NovaSeq6000 Sequencing, Library or Fragment Size Selection and Pooling | Illumina MiSeq, Illumina iSeq, Illumina NovaSeq, Covaris S220, Covaris E220, 10x Genomics (Chromium), PacBio SEQUEL IIe | NextGen DNA Sequencing | Visit NextGen DNA Sequencing: Home | Contact | Services | Instruments | Core Resources | Getting Started | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Covaris Fragmentation | Covaris Fragmentation | Covaris S220, Covaris E220 | NextGen DNA Sequencing | Contact UF | ICBR NextGen DNA Sequencing for Covaris Fragmentation: ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Covaris assisted use (One hour). No consumables provided. Call NDS lab to reserve instrument. | Covaris Fragmentation | Covaris S220, Covaris E220 | NextGen DNA Sequencing | Reservation needed for assisted use of Covaris instruments for DNA fragmentation. Two instruments are available: E210 and S2, depending on number of samples. Consumables are not provided.UF | ICBR NextGen DNA Sequencing /// ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Illumina Library Construction Services | Illumina Library Construction Services | Illumina MiSeq, Illumina iSeq, Illumina NovaSeq | NextGen DNA Sequencing | Contact UF | ICBR NextGen DNA Sequencing for Illumina Library Construction Services: ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Illumina Reduced Representation Bisulfite-Converted (RRBS) Library | Illumina Library Construction Services | Illumina MiSeq, Illumina iSeq, Illumina NovaSeq | NextGen DNA Sequencing | UF | ICBR NextGen DNA Sequencing /// ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Illumina NEB Enzymatic-based Methylation libraries | Illumina Library Construction Services | Illumina MiSeq, Illumina iSeq, Illumina NovaSeq | NextGen DNA Sequencing | Illumina: NEB enzymatic-methyl, fragment library construction: single-read, paired-end, or barcoded.UF | ICBR NextGen DNA Sequencing /// ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Illumina COVID-RVOP-seq | Illumina Library Construction Services | Illumina MiSeq, Illumina iSeq, Illumina NovaSeq | NextGen DNA Sequencing | UF | ICBR NextGen DNA Sequencing /// ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Illumina Standard library construction (NEB) | Illumina Library Construction Services | Illumina MiSeq, Illumina iSeq, Illumina NovaSeq | NextGen DNA Sequencing | Illumina Standard library construction (New England Biolabs)UF | ICBR NextGen DNA Sequencing /// ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Illumina PCR-free library construction (VWR SparQ QuantaBio) | Illumina Library Construction Services | Illumina MiSeq, Illumina iSeq, Illumina NovaSeq | NextGen DNA Sequencing | Illumina PCR-free library construction (VWR SparQ QuantaBio)UF | ICBR NextGen DNA Sequencing /// ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Illumina Low-input library construction (NEB) | Illumina Library Construction Services | Illumina MiSeq, Illumina iSeq, Illumina NovaSeq | NextGen DNA Sequencing | Illumina Low-input library construction (New England Biolabs)UF | ICBR NextGen DNA Sequencing /// ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Illumina MiSeq Sequencing | Illumina MiSeq Sequencing | Illumina MiSeq, Illumina iSeq, Illumina NovaSeq | NextGen DNA Sequencing | Contact UF | ICBR NextGen DNA Sequencing for Illumina Sequencing Services: ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Illumina MiSeq | Illumina MiSeq Sequencing | Illumina MiSeq, Illumina iSeq, Illumina NovaSeq | NextGen DNA Sequencing | Illumina MiSeq Rapid turn-around sequencing. Data provided in one of many possible means: Illumina BaseSpace, secure-server, or hard drive.UF | ICBR NextGen DNA Sequencing /// ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050
** - Max output PE per FC run (Gb) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Library or Fragment Size Selection and Pooling | Library or Fragment Size Selection and Pooling | Illumina MiSeq, Illumina iSeq, Illumina NovaSeq, PacBio SEQUEL IIe | NextGen DNA Sequencing | Contact UF | ICBR NextGen DNA Sequencing: ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| General DNA or library fragment selection by AMPure with QUBIT and TapeStation (per sample) | Library or Fragment Size Selection and Pooling | Illumina MiSeq, Illumina iSeq, Illumina NovaSeq, PacBio SEQUEL IIe | NextGen DNA Sequencing | General DNA or library fragment selection by AMPure with QUBIT and TapeStation (per sample)UF | ICBR NextGen DNA Sequencing /// ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| General DNA or library fragment selection by ELF with QUBIT and TapeStation (per sample) | Library or Fragment Size Selection and Pooling | Illumina MiSeq, Illumina iSeq, Illumina NovaSeq, PacBio SEQUEL IIe | NextGen DNA Sequencing | General DNA or library fragment selection by ELF with QUBIT and TapeStation (per sample)UF | ICBR NextGen DNA Sequencing /// ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| General DNA or library fragment selection by Gel with QUBIT and TapeStation (per sample) | Library or Fragment Size Selection and Pooling | Illumina MiSeq, Illumina iSeq, Illumina NovaSeq, PacBio SEQUEL IIe | NextGen DNA Sequencing | General DNA or library fragment selection by Gel with QUBIT and TapeStation (per sample)UF | ICBR NextGen DNA Sequencing /// ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| General Library pooling (per every three samples, add one sample evaluation per pool) | Library or Fragment Size Selection and Pooling | Illumina MiSeq, Illumina iSeq, Illumina NovaSeq, PacBio SEQUEL IIe | NextGen DNA Sequencing | General Library pooling (per every three samples, add one sample evaluation per pool)UF | ICBR NextGen DNA Sequencing /// ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Library or Sample QC and Clean-up | Library or Sample QC and Clean-up | Illumina MiSeq, Illumina iSeq, Illumina NovaSeq, PacBio SEQUEL IIe | NextGen DNA Sequencing | Contact UF | ICBR NextGen DNA Sequencing: ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| General Amplicon or fragment library evaluation: QUBIT and TapeStation (per sample) | Library or Sample QC and Clean-up | Illumina MiSeq, Illumina iSeq, Illumina NovaSeq, PacBio SEQUEL IIe | NextGen DNA Sequencing | General Amplicon or fragment library evaluation: QUBIT and TapeStation (per sample)UF | ICBR NextGen DNA Sequencing /// ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| General DNA quantification by QUBIT (per sample) | Library or Sample QC and Clean-up | Illumina MiSeq, Illumina iSeq, Illumina NovaSeq, PacBio SEQUEL IIe | NextGen DNA Sequencing | General DNA quantification by QUBIT (per sample)UF | ICBR NextGen DNA Sequencing /// ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| General MoBio PowerClean clean-up, per sample | Library or Sample QC and Clean-up | Illumina MiSeq, Illumina iSeq, Illumina NovaSeq, PacBio SEQUEL IIe | NextGen DNA Sequencing | General MoBio PowerClean clean-up, per sampleUF | ICBR NextGen DNA Sequencing /// ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Illumina NovaSeq6000 Sequencing | NovaSeq Services, Illumina NovaSeq6000 Sequencing | Illumina NovaSeq | NextGen DNA Sequencing | Contact UF | ICBR NextGen DNA Sequencing for NovaSeq Services: ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Illumina NovaSeq: S1 | NovaSeq Services, Illumina NovaSeq6000 Sequencing | Illumina NovaSeq | NextGen DNA Sequencing | Illumina NovaSeq: S1UF | ICBR NextGen DNA Sequencing /// ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050
* - Number of SE reads per FC run or lane ** - Max output PE per FC run (Gb) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Illumina NovaSeq: S2 | NovaSeq Services, Illumina NovaSeq6000 Sequencing | Illumina NovaSeq | NextGen DNA Sequencing | Illumina NovaSeq: S2UF | ICBR NextGen DNA Sequencing /// ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050
* - Number of SE reads per FC run or lane ** - Max output PE per FC run (Gb) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Illumina NovaSeq: S4 | NovaSeq Services, Illumina NovaSeq6000 Sequencing | Illumina NovaSeq | NextGen DNA Sequencing | Illumina NovaSeq: S4UF | ICBR NextGen DNA Sequencing /// ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050
* - Number of SE reads per FC run or lane ** - Max output PE per FC run (Gb) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Illumina NovaSeq, Sp | NovaSeq Services, Illumina NovaSeq6000 Sequencing | Illumina NovaSeq | NextGen DNA Sequencing | Illumina NovaSeq: SPUF | ICBR NextGen DNA Sequencing /// ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050
* - Number of SE reads per FC run or lane ** - Max output PE per FC run (Gb) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| PacBio Library Construction Services | PacBio Library Construction Services | PacBio SEQUEL IIe | NextGen DNA Sequencing | Contact UF | ICBR NextGen DNA Sequencing for PacBio Library Construction Services: ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| PacBio IsoSeq libraries | PacBio Library Construction Services | PacBio SEQUEL IIe | NextGen DNA Sequencing | PacBio IsoSeq librariesUF | ICBR NextGen DNA Sequencing /// ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| PacBio Long-insert genomic (>15 kb) libraries | PacBio Library Construction Services | PacBio SEQUEL IIe | NextGen DNA Sequencing | PacBio Long-insert genomic (>15 kb) librariesUF | ICBR NextGen DNA Sequencing /// ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| PacBio SMRT bell library for single pool of barcoded genomic (<15 Kb) | PacBio Library Construction Services | PacBio SEQUEL IIe | NextGen DNA Sequencing | PacBio SMRT bell library for single pool of barcoded genomic (<15 Kb)UF | ICBR NextGen DNA Sequencing /// ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| PacBio SMRT bell library for single pool of barcoded amplicon (<10 Kb) | PacBio Library Construction Services | PacBio SEQUEL IIe | NextGen DNA Sequencing | PacBio SMRT bell library for single pool of barcoded amplicon (<10 Kb) ICBR-NextGenSeq@ad.ufl.edu | 352.273.8050
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| PacBio SMRT bell library for single pool of barcoded 16S | PacBio Library Construction Services | PacBio SEQUEL IIe | NextGen DNA Sequencing | PacBio SMRT bell library for single pool of barcoded 16SUF | ICBR NextGen DNA Sequencing /// ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| PacBio Barcoded Genomic (<15kb) library construction, per sample (Add PacBio SMRT bell library for single pool of barcoded genomic) | PacBio Library Construction Services | PacBio SEQUEL IIe | NextGen DNA Sequencing | PacBio Barcoded Genomic (<15kb) library construction, per sample (Add PacBio SMRT bell library for single pool of barcoded genomic)UF | ICBR NextGen DNA Sequencing /// ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| PacBio Barcoded amplicon (<10 kb) library construction, per sample (Add PacBio SMRT bell library for single pool of barcoded amplicons) | PacBio Library Construction Services | PacBio SEQUEL IIe | NextGen DNA Sequencing | PacBio Barcoded amplicon (<10 kb) library construction, per sample (Add PacBio SMRT bell library for single pool of barcoded amplicons)UF | ICBR NextGen DNA Sequencing /// ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| PacBio Barcoded 16S (full-length) library construction: per sample (Add PacBio SMRT bell library for single pool of barcoded 16S) | PacBio Library Construction Services | PacBio SEQUEL IIe | NextGen DNA Sequencing | PacBio Barcoded 16S (full-length) library construction: per sample (Add PacBio SMRT bell library for single pool of barcoded 16S)UF | ICBR NextGen DNA Sequencing /// ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| PacBio SEQUEL IIe Sequencing Runs | PacBio SEQUEL IIe Sequencing Runs | PacBio SEQUEL IIe | NextGen DNA Sequencing | Contact UF | ICBR NextGen DNA Sequencing for PacBio SEQUEL Sequencing Services: ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| PacBio SEQUEL IIe single SMRT cell run (3-5 million reads) | PacBio SEQUEL IIe Sequencing Runs | PacBio SEQUEL IIe | NextGen DNA Sequencing | PacBio SEQUEL IIe single SMRT cell run (3-5 million reads)UF | ICBR NextGen DNA Sequencing /// ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050
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| Illumina iSeq Sequencing | Illumina iSeq Sequencing | Illumina iSeq | NextGen DNA Sequencing | Contact UF | ICBR NextGen DNA Sequencing for iSeq Sequencing Services: ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Illumina iSeq: 2x150 (1.2 Gb output) | Illumina iSeq Sequencing | Illumina iSeq | NextGen DNA Sequencing | Illumina iSeq: 2x150 (1.2 Gb output)UF | ICBR NextGen DNA Sequencing /// ICBR-NextGenSeq@ad.ufl.edu /// 352.273.8050
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| Back to top of page | 10x Genomics Library Construction, Consultation, 10x Genomics, Covaris Fragmentation, Data Delivery Media, General Services: Load only run & pooling, Illumina Library Construction Services, Illumina Sequenicng Services, Library or Fragment Selection, Library or sample clean-up and quantitation, Miscellaneous Services, NovaSeq Services, PacBio Library Construction Services, Sample or Library Evaluation, Workshops, Training, iSeq Sequencing Service, PacBio SEQUEL IIe Sequencing Runs, Illumina MiSeq Sequencing, Illumina iSeq Sequencing, Library or Sample QC and Clean-up, Illumina NovaSeq6000 Sequencing, Library or Fragment Size Selection and Pooling | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
UF | ICBR Proteomics & Mass Spectrometry | Direct Infusion, Gel-Related Services, Instrumental Analysis, Luminex Services, MRM Quantitative Services, Method Development, Protein Identification, Quantitative Analysis, Quantitative Proteomics, Reagents, Sample Preparation, Training, Workshops, Consultation, TSQ Altis | TSQ Altis | Proteomics & Mass Spectrometry | Visit Proteomics & Mass Spectrometry: Home | Contact | Services | Instruments | Core Resources | Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Direct Infusion | Direct Infusion | Proteomics & Mass Spectrometry | Contact UF | ICBR Proteomics & Mass Spectrometry for Direct Infusion Services: ICBR-Proteomics@ad.ufl.edu /// 352.273.8060 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Direct Infusion | Direct Infusion | Proteomics & Mass Spectrometry | Service includes directly infusing pure samples to mass spectrometer; no liquid chromatography separation.UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| Gel-Related Services | Gel-Related Services | Proteomics & Mass Spectrometry | Contact UF | ICBR Proteomics & Mass Spectrometry for Gel-Related Services: ICBR-Proteomics@ad.ufl.edu /// 352.273.8060 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 1D PAGE Services | Gel-Related Services | Proteomics & Mass Spectrometry | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot.UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| 2D PAGE Services | Gel-Related Services | Proteomics & Mass Spectrometry | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot.UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| Excise protein gel plug | Gel-Related Services | Proteomics & Mass Spectrometry | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot.UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| Isoelectric Focusing | Gel-Related Services | Proteomics & Mass Spectrometry | Isoelectric FocusingUF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| Typhoon self service per scan | Gel-Related Services | Proteomics & Mass Spectrometry | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot.UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| Instrumental Analysis | Instrumental Analysis | Proteomics & Mass Spectrometry | Contact UF | ICBR Proteomics & Mass Spectrometry for Instrument Analysis Services: ICBR-Proteomics@ad.ufl.edu /// 352.273.8060 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| HPLC (SCX, SE) | Instrumental Analysis | Proteomics & Mass Spectrometry | Sample analysis on HPLC, LCMS instruments. Per sampleUF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| LC-MS/MS - 1 h gradient for protein ID | Instrumental Analysis | Proteomics & Mass Spectrometry | 1 h gradient for protein ID. Sample analysis on HPLC, LCMS instruments. Per sample.UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| LC-MS/MS - 1 h gradient for small molecule, per sample | Instrumental Analysis | Proteomics & Mass Spectrometry | UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| LC-MS/MS - 2 hr gradient for protein ID | Instrumental Analysis | Proteomics & Mass Spectrometry | 2 hr gradient for protein ID. Sample analysis on HPLC, LCMS instruments.UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| LC-MS/MS - 3 hr gradient for protein ID | Instrumental Analysis | Proteomics & Mass Spectrometry | 3 hr gradient for protein ID. Sample analysis on HPLC, LCMS instruments.UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| LC-MS/MS - 4 hr gradient for protein ID | Instrumental Analysis | Proteomics & Mass Spectrometry | 4 hr gradient for protein IDUF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| LC-MS/MS - EvoSep One | Instrumental Analysis | Proteomics & Mass Spectrometry | LC-MS/MS - EvoSep One UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| MALDI - timsTOF fleX MS per sample | Instrumental Analysis | Proteomics & Mass Spectrometry | Sample analysis on HPLC, LCMS instruments.UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| Luminex Services | Luminex Services | Proteomics & Mass Spectrometry | Contact UF | ICBR Proteomics & Mass Spectrometry for Luminex Services: ICBR-Proteomics@ad.ufl.edu /// 352.273.8060 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Luminex Services | Luminex Services | Proteomics & Mass Spectrometry | No data analysis. Up to 39 sample slots.UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| MRM Quantitative Services | MRM Quantitative Services | Proteomics & Mass Spectrometry | Contact UF | ICBR Proteomics & Mass Spectrometry for MRM Quantitative Services Services: ICBR-Proteomics@ad.ufl.edu /// 352.273.8060 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 4000 QTRAP MRM Self-Service | MRM Quantitative Services | Proteomics & Mass Spectrometry | MRM quantitative analysis of peptides and small molecules. Price per hour.UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| MRM-TSQ Altis | MRM Quantitative Services | Proteomics & Mass Spectrometry | UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| Method Development | Method Development | Proteomics & Mass Spectrometry | Contact UF | ICBR Proteomics & Mass Spectrometry for Method Development Services: ICBR-Proteomics@ad.ufl.edu /// 352.273.8060 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Build selected ion acquisition method | Method Development | Proteomics & Mass Spectrometry | UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| Custom PTM Database Searching (add SILAC, PTM) | Method Development | Proteomics & Mass Spectrometry | Custom PTM Database Searching (add SILAC, PTM)UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| Method development for special projects, per hour | Method Development | Proteomics & Mass Spectrometry | Method development service for special projects.UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| Protein Identification | Protein Identification | Proteomics & Mass Spectrometry | Contact UF | ICBR Proteomics & Mass Spectrometry for Protein Identification Services: ICBR-Proteomics@ad.ufl.edu /// 352.273.8060 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Additional data search /sample | Protein Identification | Proteomics & Mass Spectrometry | Additional data search /sample.UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| Quantitative Analysis | Quantitative Analysis | Proteomics & Mass Spectrometry | Contact UF | ICBR Proteomics & Mass Spectrometry for Quantitative Analysis Services: ICBR-Proteomics@ad.ufl.edu /// 352.273.8060 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| iTRAQ data search | Quantitative Analysis | Proteomics & Mass Spectrometry | UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| TMT Data Search | Quantitative Analysis | Proteomics & Mass Spectrometry | UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| Quantitative Proteomics | Quantitative Proteomics | Proteomics & Mass Spectrometry | Contact UF | ICBR Proteomics & Mass Spectrometry for Quantitative Proteomics Services: ICBR-Proteomics@ad.ufl.edu /// 352.273.8060 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| iodoTMT-6 plex | Quantitative Proteomics | Proteomics & Mass Spectrometry | iodoTMT-6 plex (Up to 6 samples, 14 fractions, including LC-MS/MS and 1 data search)UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| MRM-4000 QTRAP, per sample | Quantitative Proteomics | Proteomics & Mass Spectrometry | Per sample.UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| MRM-Method development (per hour) | Quantitative Proteomics | Proteomics & Mass Spectrometry | Per hour.UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| PRM-Orbitrap fusion, per sample | Quantitative Proteomics | Proteomics & Mass Spectrometry | PRM-Orbitrap fusion, per sampleUF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| PRM-QE plus, per sample | Quantitative Proteomics | Proteomics & Mass Spectrometry | UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| Quantitative Proteomic-Label Free | Quantitative Proteomics | Proteomics & Mass Spectrometry | Quantitative Proteomic-Label Free, with PRTC internal standards. Set up fee for 1 experiment (2 to 4 samples).UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| Quantitative Proteomics | Quantitative Proteomics | Proteomics & Mass Spectrometry | UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| Reagents | Reagents | Proteomics & Mass Spectrometry | Contact UF | ICBR Proteomics & Mass Spectrometry for Reagent Services: ICBR-Proteomics@ad.ufl.edu /// 352.273.8060 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| PC Reagents | Reagents | Proteomics & Mass Spectrometry | Charge for non routine reagents for specific projects, iTRAQ kits, and etc.UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| Sample Preparation | Sample Preparation | Proteomics & Mass Spectrometry | Contact UF | ICBR Proteomics & Mass Spectrometry: ICBR-Proteomics@ad.ufl.edu /// 352.273.8060 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Abundance Protein removal (concentrates low-abundance proteins), per sample | Sample Preparation | Proteomics & Mass Spectrometry | Per sample.UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| Protein Buffer exchange (3 KDa cut-off) | Sample Preparation | Proteomics & Mass Spectrometry | Protein Buffer exchange (3 KDa cut-off)UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| Metabolite EAE Extraction | Sample Preparation | Proteomics & Mass Spectrometry | UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| EZQ protein assay per plate | Sample Preparation | Proteomics & Mass Spectrometry | Per plate.UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| Phosphopeptide enrichment | Sample Preparation | Proteomics & Mass Spectrometry | Services includes various sample preparation steps such as trypsin digestion, desalting, protein assay, and enrichment for protein modifications.UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| Other Post-translational modification enrichment (inquire) | Sample Preparation | Proteomics & Mass Spectrometry | Services includes various sample preparation steps such as trypsin digestion, desalting, protein assay, and enrichment for protein modifications. UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| Protein Extraction | Sample Preparation | Proteomics & Mass Spectrometry | UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| Protein Precipitation | Sample Preparation | Proteomics & Mass Spectrometry | UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| Solid Phase Extraction/Zip tip/Evo tip | Sample Preparation | Proteomics & Mass Spectrometry | Solid Phase Extraction/Zip tip/Evo tip, per sampleUF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| Trypsin/Lys-C in gel digestion, per sample | Sample Preparation | Proteomics & Mass Spectrometry | Trypsin/Lys-C in gel digestion, per sample. Sample preparation in protein identification.UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| Trypsin/Lys-C in solution digestion | Sample Preparation | Proteomics & Mass Spectrometry | Trypsin/Lys-C in solution digestion, per sampleUF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| Training | Consultation, Training | Proteomics & Mass Spectrometry | Contact UF | ICBR Proteomics & Mass Spectrometry: ICBR-Proteomics@ad.ufl.edu /// 352.273.8060 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| MRM Triple Quadruple Mass Spectrometer Training | Training, Consultation | Proteomics & Mass Spectrometry | Two day training to learn MRM optimization to quantify peptides and small molecules.UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| Training 2D-PAGE | Training | Proteomics & Mass Spectrometry | UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| TSQ Altis | TSQ Altis | TSQ Altis | Proteomics & Mass Spectrometry | Contact UF | ICBR Proteomics & Mass Spectrometry: ICBR-Proteomics@ad.ufl.edu /// 352.273.8060 | Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| TSQ Altis Services | TSQ Altis | TSQ Altis | Proteomics & Mass Spectrometry | The Thermo Scientific TSQ Altis Triple Quadrupole Mass Spectrometer features segmented quadrupoles with hyperbolic faces, an enhanced dual-mode discrete-dynode electron multiplier detector, and high resolution SRM (0.2 FWHM) for unprecedented sensitivity, selectivity, and speed, as well as unmatched analytical flexibility, reproducibility, and outstanding precision. The TSQ Altis enables every analytical laboratory to successfully address its most demanding applications. It enables every analytical laboratory to successfully address its most demanding applications.UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
| Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Workshops | Workshops, Consultation, Training | Proteomics & Mass Spectrometry | Contact UF | ICBR Proteomics & Mass Spectrometry: ICBR-Proteomics@ad.ufl.edu /// 352.273.8060 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Proteomics Workshop | Workshops, Consultation, Training | Proteomics & Mass Spectrometry | Inquire about availability and upcoming dates.UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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| Back to top of page | Direct Infusion, Gel-Related Services, Instrumental Analysis, Luminex Services, MRM Quantitative Services, Method Development, Protein Identification, Quantitative Analysis, Quantitative Proteomics, Reagents, Sample Preparation, Training, Workshops, TSQ Altis | TSQ Altis | Proteomics & Mass Spectrometry |
Proper Citation:
University of Florida ICBR Gene Expression and Genotyping Core Core Facility, RRID:SCR_019145
ICBR-Genotyping@ad.ufl.edu
ICBR-GeneExpression@ad.ufl.edu
(352) 273-8043
(352) 273-8036
