Monoclonal Antibody
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Overview
The Monoclonal Antibody core develops new mouse monoclonal antibodies for investigators to use in basic and applied research. Major service categories include: immunization of mice, cell fusion, cell cloning, screening by ELISA and Western blot, monoclonal antibody production, purification and labeling, monoclonal antibody analyses such as antibody/antigen affinity measurements, epitope binning and identification of matched antibody pairs. Customer training on select self-service instruments is also offered.
Core News
Introducing Core Directors’ Open Consultation Meetings
Introducing Core Directors' Open Consultation Meetings
In a continued effort to provide accessible and specialized expertise to our users, UF ICBR is proud to announce our Core Directors’ Open Consultation Meetings. Each month we will be available for a two hour walk-in session to discuss the best approaches and strategies for using ICBR technologies and expertise to drive your research forward. Our first meeting will be on Monday, October 7th from 11:00am to 1:00pm in CGRC 189. No appointment is require nor do you have to be a current ICBR user t... Read More
Instrument Spotlight: Protein Simple Wes
Instrument Spotlight: Protein Simple Wes
Wes™ lets you separate and analyze proteins by size from 2-440 kDa. He also gets you down to pg-level sensitivity with just 3 µL of starting material. Got a lot of samples and no time? No problem. Wes runs up to 25 samples in 3 hours flat and gives you quantitative, size-based data including total protein. Don’t wait to discover, get going today with Wes!
Our Simple Western systems offer the right throughput for your needs. Whether you are looking to process up to 24 samples per run or ... Read More
Is Your Monoclonal Antibody Authentic? Confirming Hybridoma Cell Line Identity by IgC cDNA Sequencing
Is Your Monoclonal Antibody Authentic? Confirming Hybridoma Cell Line Identity by IgC cDNA Sequencing
Widespread reports of irreproducibility in health sciences research has prompted the NIH to update new grant application guidelines to include a brief description of methods that will be used to ensure identity and validity of key biological and/or chemical resources used in the proposed study. In this regard there is a significant effort now underway using DNA “fingerprinting” as a way to use microsatellite repeat lengths as a powerful tool for human cell line authentication. This method ha... Read More
Staff
| Name | Title | Telephone | Room | |
|---|---|---|---|---|
| Angel Sampson, Ph.D. | Monoclonal Antibody Scientific Director | (352) 273-8039 | angel.sampson@ufl.edu | 175 CGRC |
| Shadi Bootorabi | Monoclonal Antibody Senior Biological Scientist | (352) 273-8041 | shb@ufl.edu | 171A CGRC |
| Mike Mathieu | Monoclonal Antibody Core Research Technician III | (352) 273-8040 | Mikema91@ufl.edu | 171 CGRC |