NextGen DNA Sequencing

Overview

CORE OVERVIEW

Welcome to the UF ICBR NextGen DNA Sequencing core facility. ICBR NextGen DNA Sequencing provides quality, massively parallel, high-throughput sequencing data. We currently support the following platforms: PacBio (SEQUEL IIe), Illumina (NovaSeq6000, MiSeq), Oxford NanoPore (PromethION-24, MinION), and 10X Genomics Chromium,. These instruments allow for a broad range of sequencing applications for biological research. Customers are encouraged to consult with core staff for the purpose of tailoring data acquisition to specific research questions.

Our sample prep and library construction services are offered through both the NGS and the GeneExpression/Genotyping Cores. Please contact us if the service of your interest is not listed. We strive to support most common applications.

SERVICES OVERVIEW

Don’t see the service you are looking for? Visit UF | ICBR Gene Expression & Genotyping for more options.

Sequencing Services

Library Construction Services

Sample/Library QC

General Services

Sequencing

- Illumina NovaSeq6000 (high-throughput and high-plexicity projects , any run configuration, partial lane flexibility)
- Illumina MiSeq (Standard v3 and v2 runs, Micro and Nano flow cell runs for pilot runs)
- Illumina iSeq (for shallow, pilot runs)
- PacBio SEQUEL IIe (Hi-Fi long reads for de novo genome assembly and isoform analysis, etc.)
- Oxford NanoPore PromethION-24 (Ultra-long reads for de novo genome assembly of high-throughput projects)
- Oxford NanoPore MinION (Ultra-long reads for de novo genome assembly of small genomes or pilot runs)

Library Construction Services for Major Applications (in conjunction with GE/GT core):

- Illumina (shotgun-genome, WGBS, RRBS, 16S metagenomics, COVID-seq, RNA-seq, Exome-seq)
- PacBio (large-insert genome, IsoSeq, Amplicon-seq, full-length 16S
- Oxford NanoPore (large-insert genome, full-length cDNA, Direct RNA-seq, full-length 16S, COVID-seq)
- 10X Genomics (Single-cell: RNA-Seq, ATACseq, VDJ)

Sample/Library QC

- QUBIT
- Covaris S220, E220
- qPCR
- TapeStation
- SAGE ELF

Project Consultation
Bioanalyzer, TapeStation
Qubit Assays
Assisted use of Covaris for sample fragmentation
Fragment Selection (ELF-based)
Sample/library clean-up
Load only sequencing instrument runs
Library pooling and pool quantitation

Please note that our library construction and sequencing services do not include data processing. Visit ICBR Bioinformatics to request Data Analysis services.

CORE NEWS

Important Dates for Fiscal Year End

Please note: Due to the Fiscal Year End, the last invoices to process for FY2022 will be created on Monday, June 13th. Due to Fiscal Year End, ICBR’s upcoming billing schedule will be altered as follows: 12/12/2022 – Last invoices generated in 2022 12/19/2022 at noon – Deadline to submit disputes for 2022 payment. If you have expiring funds, please work with your core laboratory contact to make sure your project is completed and billed before the 6/13/2022 invoices are posted. ...

ICBR Closed over Thanksgiving Holiday

ICBR will be closed Thursday (November 24th) and Friday (November 25th) for the Thanksgiving holiday. We here at ICBR are thankful for you, and hope you enjoy the holiday! Normal business hours will resume on Monday, November 28th. ...

Scientific expedition to study human impact on Florida Everglades

Scientific expedition to study human impact on Florida Everglades A team of five that includes a female University of Florida professor as its lead scientist will embark Thursday on a dangerous trek across the Florida Everglades to assess the impact of humans on the world’s largest subtropical wilderness. The group will retrace an 1897 canoe journey that was first completed by explorer and scientist Hugh de Laussat Willoughby. Tracie Baker, an associate professor of environmental and global h...

Big Changes for ICBR Electron Microscopy Core in 2023

We are excited to announce that our EM core is going to be relocated entirely in CGRC. The move should not only offer our users additional convenience with a singular location and increased proximity to our peer cores, but the renovation will provide our users with a revamped lab space that will expand the capabilities and offerings of our EM team. We are very excited about the next steps, but there is still a lot of work left to do. EM is currently doing their best to facilitate this move while...

ICBR Announces Special Pilot Grant Opportunity

DEADLINE EXTENDED: ICBR Announces Special Pilot Grant Opportunity The mission of ICBR is to champion and enable scientific discovery and innovation throughout the University of Florida research community by providing access to world-class biotechnology expertise and instrumentation. To introduce the UF research community to the use of Oxford Nanopore Technology PromethION nanopore DNA sequencing, the ICBR is requesting pilot project proposals utilizing this technology for funding consideration. ...

Repeat performance: UF again ranked No. 5 among public universities by U.S. News & World Report

Repeat performance: UF again ranked No. 5 among public universities by U.S. News & World Report UF News For the second year in a row, the University of Florida is listed as one of the top five public research universities in the nation, according to the 2023 Best Colleges rankings released today by U.S. News & World Report. The No. 5 recognition reaffirms UF’s status as an academic powerhouse in research, innovation, educational excellence, student outcomes and technology transfer. ...

Study uncovers differences in saliva bacteria of students with recent suicidal thoughts

Study uncovers differences in saliva bacteria of students with recent suicidal thoughts Samantha Murray A new University of Florida study has found that bacteria in the saliva of college students who reported recent thoughts of suicide differed in significant ways from those found in students who had not experienced recent suicidal thinking. While there is a growing body of research on mental health and the human microbiome, this is the first study to look at bacterial differences in the sa...

Ant queens control insulin to boost lifespan, reproduction

Ant queens control insulin to boost lifespan, reproduction Eric Hamilton In most of the animal world, there’s a sad trade-off to make: The more babies you have, the shorter you live. But ants buck the system. The queens — the only individuals in a nest that reproduce — also live five, 10, up to 30 times longer than their genetically identical worker sisters. How do they pull off what the rest of animalkind cannot? A new study from University of Florida biologist Hua Yan and his collea...

CORE INSTRUMENTS