NextGen DNA Sequencing
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NEXTGEN DNA SEQUENCING: Home | Staff & Contact | Instruments | Services & Prices | Getting Started | Sample Requirements | Core Resources
Overview
Welcome to the UF ICBR NextGen DNA Sequencing core facility. The ICBR-NGS core provides researchers with quality, massively parallel, high-throughput sequencing data using the most current instrumentation, at a reasonable cost. We currently support the most popular platforms: Illumina NextSeq500 and MiSeq, HiSeq3000 and the Pacific Biosciences SEQUEL system. Together, these instruments cover a broad range of powerful applications. Our free consultation services aim to help researchers navigate through the complex matrix of experimental options represented by the available sequencing technologies. Some of these important considerations include: read length, error rate, predominant type of error, data output/run, speed, cost, etc. The ever-broadening range of applications include (but are not limited to) de novo sequencing of whole genomes, targeted sequencing, transcriptome sequencing (RNA-Seq), chromatin immunoprecipitation (ChIP) sequencing, methylation analysis and metagenomics.
Our sample prep and library construction services are offered through both the NGS and the GeneExpression/Genotyping Cores. Please contact us if the service of your interest is not listed. We strive to support most common applications.
ICBR NEXTGEN DNA Sequencing Services
Don't see the service you are looking for? Visit ICBR Gene Expression & Genotyping for more options.
Sequencing Services
Library Construction Services
General Services
NovaSeq 6000 Sequencing
- Request services for: Sequencing formats (S4 2×150, S4 2×100, S2 2×150, S2 2×100, S2 2×50, S1 2×150, S1 2×100, S1 2×50, SP 2×250, SP 2×150, SP 2×50)
MiSeq Illumina Sequencing
- Request services for: Sequencing formats (2x300v3, 2x250v2, 2x150v2, 2x75v3, 1x300v2, 1x150v3, 1x50v2)
NextSeq500 Illumina Sequencing
- Request services for: Sequencing formats (HIGH output: 2×150, 2×75, 1×150, 1×75. MID output: 2×150, 2×75, 1×150)
PacBio SEQUEL Sequencing
- Sequencing formats (10-hour movies, 20-hour movies). LR v3 SMRT cells
- Current chemistry (early 2019): v3. SMRT Link 6.0
- Reads per SMRT cell run: 400k-600k reads
- Illumina Standard DNA-Seq
- Illumina PCR-free DNA-Seq
- Illumina Whole Genome Bisulfite-converted WGBS)
- Illumina Reduced Representation Bisulfite converted (RRBS)
- Illumina Ultra-low input DNA-Seq
- Illumina ATAC-seq
- 10X Genomics linked-reads
- 10X Genomics ATAC-Seq
- PacBio Long-insert DNA-Seq for de novo assemblies
- PacBio Barcoded bacterial DNA for multiplex sequencing
- PacBio Barcoded amplicon-Seq (up to 10 Kb)
- PacBio IsoSeq for transcriptome/isoform analysis
- PacBio Full-length 16S metagenomics
- Project Consultation
- Bioanalyzer, TapeStation
- Qubit Assays
- Assisted use of Covaris for sample fragmentation
- Fragment Selection (ELF-based)
- Sample/library clean-up
- Load only sequencing instrument runs
- Library pooling and pool quantitation
Please note that our library construction and sequencing services do not include data processing. Visit ICBR Bioinformatics to request Data Analysis services.
NextGen DNA Sequencing News
UF | ICBR Scientists Contribute to Clinical Trial Testing Novel Treatment for Canine Atopic Dermatitis
UF | ICBR Scientists Contribute to Clinical Trial Testing Novel Treatment for Canine Atopic Dermatitis
Atopic dermatitis, or AD, is a common chronic inflammatory skin disease affecting dogs, causing defects of the skin barrier and modifications of the normal cutaneous microflora. While multiple treatment options are available, there is a growing interest in the use of alternative natural ingredient products. In human AD reports show the potential benefits of topical applications of heat-killed beneficial bacteria, with some studies demonstrating the efficacy of rapidly killed beneficial la... Read More
UF researchers to use AI to predict how hurricanes spread invasive plants
UF researchers to use AI to predict how hurricanes spread invasive plants
Courtesy of UF/IFAS
Written by Brad Buck
Scientists project hurricane intensity and frequency will increase with climate change. That leads researchers to want to better predict how storms will disperse and establish nonnative plant invaders.
Knowing where invasive plants spread will always be useful. But it may become especially handy soon as the National Hurricane Center is considering moving the official start of the Atlantic hurricane season two weeks earlier, starting it May 15, instead of ... Read More
World-Renowned UF Health Biochemistry Researcher Passes Away
World-Renowned UF Health Biochemistry Researcher Passes Away
Courtesy of College of Medicine News
March 3, 2021 — University of Florida Health biochemistry professor Mavis Agbandje-McKenna, Ph.D., whose world-renowned work on the detailed structure of viruses led to advances in gene therapy treatments for different diseases, died Wednesday at her home near Gainesville of the neurodegenerative disease amyotrophic lateral sclerosis. She was 57.
Agbandje-McKenna’s work with the small, infectious particles had a worldwide impact, allowing other scientists to ... Read More
UF Health pilot program to deliver COVID-19 monoclonal antibody therapy to rural communities
UF Health pilot program to deliver COVID-19 monoclonal antibody therapy to rural communities
Courtesy of UF Health
Written by Diana Tonnessen
A University of Florida Health pilot program will bring a new COVID-19 monoclonal antibody therapy to rural communities in North Florida during the coming weeks.
As part of the White House’s Operation Warp Speed initiative, the U.S. Department of Health and Human Services selected UF Health and the UF Clinical and Translational Science Institute to coordinate the distribution and administration of 1,000 doses of bamlanivimab, a monoclonal antibody... Read More
UF offers powerful new NVIDIA supercomputer to students and researchers across State University System
UF offers powerful new NVIDIA supercomputer to students and researchers across State University System
Courtesy of UF NEWS
Written by Brittany Wise
University of Florida’s new supercomputer — the fastest artificial intelligence supercomputer in higher education — will soon be available for students and faculty across the State University System, UF Provost Joe Glover announced today.
The computer will give faculty and students from across the State unparalleled computing power in artificial intelligence (AI) to apply across many areas to improve lives, bolster industry, and create ec... Read More
New Approach Isolating Stem Cells Could Lead to Breakthrough In Regenerative Medicine
New Approach Isolating Stem Cells Could Lead to Breakthrough In Regenerative Medicine
A recent study conducted at the University of Florida could transform the way we isolate stem cells, validating a method that takes only minutes and can be performed just about anywhere. Stem cells are “immature” cells that have the unique ability to develop into other cell types. For example, hematopoietic stem cells (HSCs) regenerate blood cells and mesenchymal stem cells (MSCs) regenerate cells of solid tissues. Because HSCs and MSCs are often used for clinical regenerative medicine, isolati... Read More
Novel CD33 Antibodies Unravel Localization, Biology, and Therapeutic Implications of CD33 Isoforms
Novel CD33 Antibodies Unravel Localization, Biology, and Therapeutic Implications of CD33 Isoforms
UF | ICBR Drs. Angel Sampson and Andria Doty recently teamed up with Dr. Jatinder Lamba in the Department of Pharmacotherapy and Translational Research in the UF College of Pharmacy, and Dr. Soheil Meshinchi at the Fred Hutchinson Cancer Research Center, University of Washington, in an effort to further understand the therapeutic relevance of various forms of the CD33 protein as it relates to the antibody-drug therapy. CD33 is expressed on malignant cancer cells in nearly all patients with ac... Read More
Featured Video: Black Voices in Research
Featured Video: Black Voices in Research
As part of its mission to facilitate honest and vulnerable conversations about race, the University of Florida Clinical and Translational Science Institute Diversity and Cultural Competence Council (UF CTSI DC3) presents its inaugural Black Voices in Research Storytelling Event. This event and others like it will serve to underscore the inherent value of sharing personal stories and the role that these stories can play in creating and promoting an inclusive, diverse, equitable, and accessible ... Read More
Instruments
Staff
| Name | Title | Telephone | Room | |
|---|---|---|---|---|
| David Moraga | NextGen DNA Sequencing Scientific Director | (352) 273-8053 | moraga@ufl.edu | 178J CGRC |
| Nedka Panayotova | NextGen DNA Sequencing Senior Biological Scientist | (352) 273-8050 | nedpan@ufl.edu | 178 CGRC |
| Lydia Von Borstel, B.S. | NextGen DNA Sequencing Core Research Technician III | (352) 273-8050 | lvonborstel@ufl.edu | 178 CGRC |
| Benjamin Hoffman | NextGen DNA Sequencing Core Research Technician III | (352) 273-8050 | semplar@ufl.edu | 178 CGRC |
| Diansy Zincke | NextGen DNA Sequencing Core Research Technician II | (352) 273-8050 | dzincke@ufl.edu | 178 CGRC |
| Xiao Hui Zhou | NextGen DNA Sequencing Core Research Technician II | (352) 273-8050 | zxh@ufl.edu | 178 CGRC |