NextGen DNA Sequencing

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Overview

Welcome to the UF ICBR NextGen DNA Sequencing core facility. The ICBR-NGS core provides researchers with quality, massively parallel, high-throughput sequencing data using the most current instrumentation, at a reasonable cost. We currently support the most popular platforms: Illumina NextSeq500 and MiSeq, HiSeq3000 and the Pacific Biosciences SEQUEL system. Together, these instruments cover a broad range of powerful applications. Our free consultation services aim to help researchers navigate through the complex matrix of experimental options represented by the available sequencing technologies. Some of these important considerations include: read length, error rate, predominant type of error, data output/run, speed, cost, etc. The ever-broadening range of applications include (but are not limited to) de novo sequencing of whole genomes, targeted sequencing, transcriptome sequencing (RNA-Seq), chromatin immunoprecipitation (ChIP) sequencing, methylation analysis and metagenomics.

Our sample prep and library construction services are offered through both the NGS and the GeneExpression/Genotyping Cores. Please contact us if the service of your interest is not listed. We strive to support most common applications.

ICBR NEXTGEN DNA Sequencing Services

Don't see the service you are looking for? Visit ICBR Gene Expression & Genotyping for more options.

Sequencing Services

Library Construction Services

General Services

NovaSeq 6000 Sequencing

  • Request services for: Sequencing formats (S4 2×150, S4 2×100, S2 2×150, S2 2×100, S2 2×50, S1 2×150, S1 2×100, S1 2×50, SP 2×250, SP 2×150, SP 2×50)

MiSeq Illumina Sequencing 

  • Request services for: Sequencing formats (2x300v3, 2x250v2, 2x150v2, 2x75v3, 1x300v2, 1x150v3, 1x50v2)

NextSeq500 Illumina Sequencing 

  • Request services for: Sequencing formats (HIGH output: 2×150, 2×75, 1×150, 1×75. MID output: 2×150, 2×75, 1×150)

PacBio SEQUEL Sequencing 

  • Sequencing formats (10-hour movies, 20-hour movies). LR v3 SMRT cells
  • Current chemistry (early 2019): v3. SMRT Link 6.0
  • Reads per SMRT cell run: 400k-600k reads
  • Illumina Standard DNA-Seq
  • Illumina PCR-free DNA-Seq
  • Illumina Whole Genome Bisulfite-converted WGBS)
  • Illumina Reduced Representation Bisulfite converted (RRBS)
  • Illumina Ultra-low input DNA-Seq
  • Illumina ATAC-seq
  • 10X Genomics linked-reads
  • 10X Genomics ATAC-Seq
  • PacBio Long-insert DNA-Seq for de novo assemblies
  • PacBio Barcoded bacterial DNA for multiplex sequencing
  • PacBio Barcoded amplicon-Seq (up to 10 Kb)
  • PacBio IsoSeq for transcriptome/isoform analysis
  • PacBio Full-length 16S metagenomics
  • Project Consultation
  • Bioanalyzer, TapeStation
  • Qubit Assays
  • Assisted use of Covaris for sample fragmentation
  • Fragment Selection (ELF-based)
  • Sample/library clean-up
  • Load only sequencing instrument runs
  • Library pooling and pool quantitation

Please note that our library construction and sequencing services do not include data processing. Visit ICBR Bioinformatics to request Data Analysis services.

NextGen DNA Sequencing News

UF | ICBR Scientists Contribute to Clinical Trial Testing Novel Treatment for Canine Atopic Dermatitis UF | ICBR Scientists Contribute to Clinical Trial Testing Novel Treatment for Canine Atopic Dermatitis
UF | ICBR Scientists Contribute to Clinical Trial Testing Novel Treatment for Canine Atopic Dermatitis
  Atopic dermatitis, or AD, is a common chronic inflammatory skin disease affecting dogs, causing defects of the skin barrier and modifications of the normal cutaneous microflora. While multiple treatment options are available, there is a growing interest in the use of alternative natural ingredient products. In human AD reports show the potential benefits of topical applications of heat-killed beneficial bacteria, with some studies demonstrating the efficacy of rapidly killed beneficial la...
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UF | ICBR Scientists Contribute to Clinical Trial Testing Novel Treatment for Canine Atopic Dermatitis
UF researchers to use AI to predict how hurricanes spread invasive plants UF researchers to use AI to predict how hurricanes spread invasive plants
UF researchers to use AI to predict how hurricanes spread invasive plants
Courtesy of UF/IFAS Written by Brad Buck Scientists project hurricane intensity and frequency will increase with climate change. That leads researchers to want to better predict how storms will disperse and establish nonnative plant invaders. Knowing where invasive plants spread will always be useful. But it may become especially handy soon as the National Hurricane Center is considering moving the official start of the Atlantic hurricane season two weeks earlier, starting it May 15, instead of ...
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UF researchers to use AI to predict how hurricanes spread invasive plants
World-Renowned UF Health Biochemistry Researcher Passes Away World-Renowned UF Health Biochemistry Researcher Passes Away
World-Renowned UF Health Biochemistry Researcher Passes Away
Courtesy of College of Medicine News March 3, 2021 — University of Florida Health biochemistry professor Mavis Agbandje-McKenna, Ph.D., whose world-renowned work on the detailed structure of viruses led to advances in gene therapy treatments for different diseases, died Wednesday at her home near Gainesville of the neurodegenerative disease amyotrophic lateral sclerosis. She was 57. Agbandje-McKenna’s work with the small, infectious particles had a worldwide impact, allowing other scientists to ...
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World-Renowned UF Health Biochemistry Researcher Passes Away
UF Health pilot program to deliver COVID-19 monoclonal antibody therapy to rural communities UF Health pilot program to deliver COVID-19 monoclonal antibody therapy to rural communities
UF Health pilot program to deliver COVID-19 monoclonal antibody therapy to rural communities
Courtesy of UF Health Written by Diana Tonnessen A University of Florida Health pilot program will bring a new COVID-19 monoclonal antibody therapy to rural communities in North Florida during the coming weeks. As part of the White House’s Operation Warp Speed initiative, the U.S. Department of Health and Human Services selected UF Health and the UF Clinical and Translational Science Institute to coordinate the distribution and administration of 1,000 doses of bamlanivimab, a monoclonal antibody...
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UF Health pilot program to deliver COVID-19 monoclonal antibody therapy to rural communities
UF offers powerful new NVIDIA supercomputer to students and researchers across State University System UF offers powerful new NVIDIA supercomputer to students and researchers across State University System
UF offers powerful new NVIDIA supercomputer to students and researchers across State University System
Courtesy of UF NEWS Written by Brittany Wise University of Florida’s new supercomputer — the fastest artificial intelligence supercomputer in higher education — will soon be available for students and faculty across the State University System, UF Provost Joe Glover announced today. The computer will give faculty and students from across the State unparalleled computing power in artificial intelligence (AI) to apply across many areas to improve lives, bolster industry, and create ec...
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UF offers powerful new NVIDIA supercomputer to students and researchers across State University System
New Approach Isolating Stem Cells Could Lead to Breakthrough In Regenerative Medicine New Approach Isolating Stem Cells Could Lead to Breakthrough In Regenerative Medicine
New Approach Isolating Stem Cells Could Lead to Breakthrough In Regenerative Medicine
A recent study conducted at the University of Florida could transform the way we isolate stem cells, validating a method that takes only minutes and can be performed just about anywhere. Stem cells are “immature” cells that have the unique ability to develop into other cell types.  For example, hematopoietic stem cells (HSCs) regenerate blood cells and mesenchymal stem cells (MSCs) regenerate cells of solid tissues. Because HSCs and MSCs are often used for clinical regenerative medicine, isolati...
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New Approach Isolating Stem Cells Could Lead to Breakthrough In Regenerative Medicine
Novel CD33 Antibodies Unravel Localization, Biology, and Therapeutic Implications of CD33 Isoforms Novel CD33 Antibodies Unravel Localization, Biology, and Therapeutic Implications of CD33 Isoforms
Novel CD33 Antibodies Unravel Localization, Biology, and Therapeutic Implications of CD33 Isoforms
UF | ICBR  Drs. Angel Sampson and Andria Doty recently teamed up with Dr. Jatinder Lamba in the Department of Pharmacotherapy and Translational Research in the UF College of Pharmacy, and  Dr. Soheil Meshinchi at the Fred Hutchinson Cancer Research Center, University of Washington, in an effort to further understand the therapeutic relevance of various forms of the CD33 protein as it relates to the antibody-drug therapy.  CD33 is expressed on malignant cancer cells in nearly all patients with ac...
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Novel CD33 Antibodies Unravel Localization, Biology, and Therapeutic Implications of CD33 Isoforms
Featured Video: Black Voices in Research Featured Video: Black Voices in Research
Featured Video: Black Voices in Research
 As part of its mission to facilitate honest and vulnerable conversations about race, the University of Florida Clinical and Translational Science Institute Diversity and Cultural Competence Council (UF CTSI DC3) presents its inaugural Black Voices in Research Storytelling Event. This event and others like it will serve to underscore the inherent value of sharing personal stories and the role that these stories can play in creating and promoting an inclusive, diverse, equitable, and accessible ...
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Featured Video: Black Voices in Research

Instruments

Staff

NameTitleTelephoneE-mailRoom
David MoragaNextGen DNA Sequencing Scientific Director(352) 273-8053moraga@ufl.edu178J CGRC
Nedka PanayotovaNextGen DNA Sequencing Senior Biological Scientist(352) 273-8050nedpan@ufl.edu178 CGRC
Lydia Von Borstel, B.S.NextGen DNA Sequencing Core Research Technician III(352) 273-8050lvonborstel@ufl.edu178 CGRC
Benjamin HoffmanNextGen DNA Sequencing Core Research Technician III(352) 273-8050semplar@ufl.edu178 CGRC
Diansy ZinckeNextGen DNA Sequencing Core Research Technician II(352) 273-8050dzincke@ufl.edu178 CGRC
Xiao Hui ZhouNextGen DNA Sequencing Core Research Technician II(352) 273-8050zxh@ufl.edu178 CGRC