NextGen DNA Sequencing

ICBR-NextGenSeq@ad.ufl.edu   ///  (352) 273-8050   ///    RRID:SCR_019152
High-throughput Sequencing Services, Library Construction, Fragmentation and More

Overview

CORE OVERVIEW

Welcome to the UF ICBR NextGen DNA Sequencing core facility. ICBR NextGen DNA Sequencing provides quality, massively parallel, high-throughput sequencing data. We currently support the following platforms: PacBio (SEQUEL IIe), Illumina (NovaSeq X Plus, MiSeq), Oxford NanoPore (PromethION-24, MinION Mk1c). These instruments allow for a broad range of sequencing applications for biological research. Customers are encouraged to consult with core staff for the purpose of tailoring data acquisition to specific research questions.

Our sample prep and library construction services are offered through both the NGS and the Gene Expression/Genotyping Cores. Please contact us if the service of your interest is not listed. We strive to support most common applications.

SERVICES OVERVIEW

Don’t see the service you are looking for? Visit UF | ICBR Gene Expression & Genotyping for more options.

Sequencing Services

Library Construction Services

Sample/Library QC

General Services

Sequencing

    • Illumina NovaSeq X Plus (high-throughput and high-plexicity projects , any run configuration, partial lane flexibility)
    • Illumina MiSeq (Standard v3 and v2 runs, Micro and Nano flow cell runs for pilot runs)
    • Illumina iSeq (for shallow, pilot runs)
    • PacBio SEQUEL IIe (Hi-Fi long reads for de novo genome assembly and isoform analysis, etc.)
    • Oxford NanoPore PromethION-24 (Ultra-long reads for de novo genome assembly of high-throughput projects)
    • Oxford NanoPore MinION (Ultra-long reads for de novo genome assembly of small genomes or pilot runs)

Library Construction Services for Major Applications (in conjunction with GE/GT core):

    • Illumina (shotgun-genome, WGBS, RRBS, 16S metagenomics, COVID-seq, RNA-seq, Exome-seq)
    • PacBio (large-insert genome, IsoSeq, Amplicon-seq, full-length 16S)
    • Oxford NanoPore (large-insert genome, full-length cDNA, Direct RNA-seq, full-length 16S, COVID-seq)
    • 10X Genomics (Single-cell: RNA-Seq, ATACseq, VDJ)

 

Sample/Library QC

    • QUBIT
    • Covaris S220, E220
    • qPCR
    • TapeStation
    • SAGE ELF
  • Project Consultation
  • TapeStation
  • Qubit Assays
  • Assisted use of Covaris for sample fragmentation
  • Fragment Selection (ELF-based)
  • Sample/library clean-up
  • Load only sequencing instrument runs
  • Library pooling and pool quantitation

Please note that our library construction and sequencing services do not include data processing. Visit ICBR Bioinformatics to request Data Analysis services.

CORE NEWS

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Turning Up the Heat on Tumors – ICBR Supports Landmark Study in Novel Cancer Vaccine Strategy The ICBR Cytometry and Electron Microscopy Shared Resources played an integral part in supporting a landmark study from UF cancer researchers published in the May 1 issue of the prestigious journal Cell.  The study, led by physician scientist Dr. Elias Sayour, a UF Health pediatric oncologist and co-leader of the UF Health Cancer Center’s Immuno-Oncology and Microbiome research program, represents...
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Due to the Fiscal Year End, the last invoices to process for FY2024 will be created on Monday, June 12th Due to Fiscal Year End, ICBR’s upcoming billing schedule will be altered as follows: 06/10/2024 – Last invoices generated in fiscal year 2024 06/17/2024 at noon – Deadline to submit disputes for FY2024 payment. 07/01/2024 – Invoices will begin to be created for FY2025 If you have expiring funds, please work with your core laboratory contact to make sure your project is completed and billed ...
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Advancing Spatial Transcriptomics Research with 10x Genomics Visium CytAssist The UF Interdisciplinary Center for Biotechnology Research (ICBR) is excited to announce that the 10x Genomics Visum CytAssist will be arriving to our Gene Expression and Genotyping core in May to help advance research in understanding mRNA and protein expression in normal and diseased tissue. The relationship between cells and their relative locations within tissue is critical to understanding normal development and d...
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CORE INSTRUMENTS

NextGen DNA Sequencing

CORE PERSONNEL

NameTelephoneE-mailRoom

UF | ICBR Administration

Administration
Name/Title/DepartmentPhoneEmailLocationAdministration

Dr. Anna-Lisa Paul
Director, UF | ICBR

Administration

(352) 273-8029alp@ufl.edu160 CGRCAdministration

Dr. Steven Madore
Associate Director of Science, UF | ICBR
Administration

(352) 273-8038smadore@ufl.edu156 CGRCAdministration

Nadia Brown
Assistant Director of Operations, UF | ICBR
Administration

(352) 273-9461nbahadori@ufl.edu161 CGRCAdministration



Administration

UF | ICBR Business Services

Business Services
Name/Title/DepartmentPhoneEmailLocationBusiness Services
Nadia Brown 
Assistant Director of Operations
Business Services
(352) 273-9461nbahadori@ufl.edu161 CGRCBusiness Services
Connie Caudle
Finance Manager 
Business Services
(352) 273-8027ccaudle@ufl.edu163 CGRCBusiness Services
Erica Boyd
Human Resources Generalist III
Business Services
(352) 273-8271ericalboyd@ufl.edu158 CGRCBusiness Services
Tina Hasko
Project Manager I
Business Services
(352) 273-8048thasko@ufl.edu181 CGRCBusiness Services
Mackenzie Madore
Administrative Specialist II
Business Services
(352) 273-8065mmadore@ufl.edu157 CGRCBusiness Services
Tensi Krpan
Administrative Specialist II
Business Services
(352) 273-8026tenser9@ufl.edu162 CGRCBusiness Services

Mason McDermid
Marketing & Communications Specialist
Business Services

(352) 273-8168masonmcdermid@ufl.edu154 CGRCBusiness Services



Business Services

UF | ICBR Cyberinfrastructure

Cyberinfrastructure
Name/Title/DepartmentPhoneEmailLocationCyberinfrastructure
Clinton Collins
Cyberinfrastructure Director
Cyberinfrastructure
(352) 273-9274clintc@ufl.edu155 CGRCCyberinfrastructure
Ben Thebaut
IT Analyst II
Cyberinfrastructure
(352) 273-6814ben.thebaut@ufl.edu188 CGRCCyberinfrastructure
Cameron Smith
Systems Administrator/Programmer II
Cyberinfrastructure 
(352) 273-9651pc.smith@ufl.edu188 CGRCCyberinfrastructure
Daniel Tarlini 
IT Analyst I
Cyberinfrastructure
(352) 273-8054tarlinid@ufl.edu188 CGRCCyberinfrastructure
Michael Harrison
End User Computing Specialist III
Cyberinfrastructure
(352) 273-8170mdharrison@ufl.edu188 CGRCCyberinfrastructure



Cyberinfrastructure

UF | ICBR Bioinformatics

Email Core

Bioinformatics
Name/Title/DepartmentPhoneEmailLocationBioinformatics
Dr. Alberto Riva
Scientific Director
Bioinformatics
(352) 294-5475ariva@ufl.edu191 CGRCBioinformatics
Dr. Fahong Yu
Scientist
Bioinformatics
(352) 273-8064fyu@ufl.edu191 CGRCBioinformatics
Dr. Massimiliano Tagliamonte
Bioinformatics Analyst III
Bioinformatics
(352) 273-8049mstagliamonte@ufl.edu191 CGRCBioinformatics



Bioinformatics

UF | ICBR Cytometry

Email Core

Cytometry
Name/Title/DepartmentPhoneEmailLocationCytometry
Dr. Mariza Miranda
Scientific Director
Cytometry
(352) 273-8185marizamiranda@ufl.edu292 CGRCCytometry
Doug Smith
Senior Laboratory Technician  
Cytometry
(352) 392-1745dugsmith@ufl.eduLG-164Cytometry
Kimberly Backer-Kelley
Core Research Facility Technician III
Cytometry 
(352) 273-6032klbk@ufl.edu292 CGRCCytometry
Dr. Daniil Shabashvili
Core Research Specialist II
Cytometry
(352) 273-8186

danshaba@ufl.edu

292 CGRCCytometry
Brittany Rein
Core Research Facility Technician III
Cytometry
(352) 273-6032reinb@ufl.edu292 CGRCCytometry
Dale Joseph
Core Research Facility Specialist I
Cytometry
(352) 273-6032da.joseph@ufl.edu292 CGRCCytometry



Cytometry

UF | ICBR Electron Microscopy

Email Core

Electron Microscopy
Name/Title/DepartmentPhoneEmailLocationElectron Microscopy
Paul R. Chipman
Scientific Director
Electron Microscopy
(352) 294-1790pchipman@ufl.edu184 CGRCElectron Microscopy
Karen Kelley
Core Research Facility Specialist III
Electron Microscopy
(352) 273-8547vau@ufl.edu185 CGRCElectron Microscopy
Rudy Alvarado
Core Research Facility Specialist I
Electron Microscopy
(352) 846-0251ralvaradojr@ufl.edu184 CGRCElectron Microscopy
Mary Gragg
Core Research Facility Technician III
Electron Microscopy
(352) 392-1184mary.gragg@ufl.edu184 CGRCElectron Microscopy
Quinnton Cooper
OPS Technician 
Electron Microscopy
(352) 392-1184qcooper@ufl.edu 184 CGRCElectron Microscopy



Electron Microscopy

UF | ICBR Gene Expression & Genotyping

Email Core

Gene Expression & Genotyping
Name/Title/DepartmentPhoneEmailLocationGene Expression & Genotyping
Dr. Yanping Zhang
Scientific Director
Gene Expression & Genotyping
(352) 273-8043yanp@ufl.edu178F CGRCGene Expression & Genotyping
Mark Renshaw
Core Research Facility Specialist II
Gene Expression & Genotyping
(352) 273-8036marenshaw@ufl.edu178 CGRCGene Expression & Genotyping
Dante Garcia
OPS Lab Technician
Gene Expression & Genotyping
(352) 273-8036dgarcia6@ufl.edu178 CGRCGene Expression & Genotyping
Sewande Mudiyanselage
Student Assistant
Gene Expression & Genotyping
(352) 273-8036s.mudiyanselage@ufl.edu178 CGRCGene Expression & Genotyping
Dr. Ru Dai
Core Research Facility Specialist II
Gene Expression & Genotyping
(352) 273-8036rudai@ufl.edu178 CGRCGene Expression & Genotyping
Brandon Wilson 
Core Research Facility Technician III
Gene Expression & Genotyping
(352) 273-8036brandonkwilson@ufl.edu178 CGRCGene Expression & Genotyping



Gene Expression & Genotyping

UF | ICBR Monoclonal Antibody

Email Core

Monoclonal Antibody
Name/Title/DepartmentPhoneEmailLocationMonoclonal Antibody
Dr. Angel Sampson
Scientific Director
Monoclonal Antibody
(352) 273-8039angel.sampson@ufl.edu175 CGRCMonoclonal Antibody
Shadi Bootorabi
Senior Biological Scientist
Monoclonal Antibody
(352) 273-8041shb@ufl.edu171A CGRCMonoclonal Antibody
Asher Shoucair
Core Research Facility Technician III
Monoclonal Antibody
(352) 273-8040ashoucair@ufl.edu171 CGRCMonoclonal Antibody



Monoclonal Antibody

UF | ICBR NextGen DNA Sequencing

Email Core

NextGen DNA Sequencing
Name/Title/DepartmentPhoneEmailLocationNextGen DNA Sequencing
Dr. Diansy Zincke
Scientific Director 
NextGen DNA Sequencing
(352) 273-8053dzincke@ufl.edu178 CGRCNextGen DNA Sequencing
Shanmugam Rajasekar
Core Research Facility Specialist II
NextGen DNA Sequencing
(352) 273-8068srajasekar1@ufl.edu178 CGRCNextGen DNA Sequencing
Dr. Liming Zhao
Core Research Facility Specialist II
NextGen DNA Sequencing
(352) 273-8050lmzhao@ufl.edu178 CGRCNextGen DNA Sequencing
Xiao Hui Zhou
Core Research Facility Technician III
NextGen DNA Sequencing
(352) 273-8050zxh@ufl.edu178 CGRCNextGen DNA Sequencing
Benjamin Hoffman
Core Research Facility Technician III
NextGen DNA Sequencing
(352) 273-8050semplar@ufl.edu178 CGRCNextGen DNA Sequencing
Chiara Licata
OPS Lab Technician 
NextGen DNA Sequencing
(352) 273-8050chiaralicata@ufl.edu178 CGRCNextGen DNA Sequencing
Gabrielle Johnson
OPS Lab Technician
NextGen DNA Sequencing
(352) 273-8050gabriellejohnson@ufl.edu178 CGRCNextGen DNA Sequencing



NextGen DNA Sequencing

UF | ICBR Proteomics & Mass Spectrometry

Email Core

Proteomics & Mass Spectrometry
Name/Title/DepartmentPhoneEmailLocationProteomics & Mass Spectrometry
Dr. Jin Koh
Scientific Director
Proteomics & Mass Spectrometry
(352) 273-8059jinkoh@ufl.edu168A CGRCProteomics & Mass Spectrometry
Ran Zheng
Core Research Facility Specialist II
Proteomics & Mass Spectrometry
(352) 273-8061ranzheng@ufl.edu169 CGRCProteomics & Mass Spectrometry
Ning Zhu
Core Research Facility Specialist II
Proteomics & Mass Spectrometry
(352) 273-8067nzhu@ufl.edu169 CGRCProteomics & Mass Spectrometry
Xinyue (Serena) Wang 
OPS Technician 
Proteomics & Mass Spectrometry
(352) 273-8060xinyue.wang@ufl.edu
169 CGRCProteomics & Mass Spectrometry