NextGen DNA Sequencing

Overview

CORE OVERVIEW

Welcome to the UF ICBR NextGen DNA Sequencing core facility. ICBR NextGen DNA Sequencing provides quality, massively parallel, high-throughput sequencing data. We currently support the following platforms: PacBio (SEQUEL IIe), Illumina (NovaSeq X Plus, MiSeq), Oxford NanoPore (PromethION-24, MinION Mk1c). These instruments allow for a broad range of sequencing applications for biological research. Customers are encouraged to consult with core staff for the purpose of tailoring data acquisition to specific research questions.

Our sample prep and library construction services are offered through both the NGS and the Gene Expression/Genotyping Cores. Please contact us if the service of your interest is not listed. We strive to support most common applications.

SERVICES OVERVIEW

Don’t see the service you are looking for? Visit UF | ICBR Gene Expression & Genotyping for more options.

Sequencing Services

Library Construction Services

Sample/Library QC

General Services

Sequencing

- Illumina NovaSeq X Plus (high-throughput and high-plexicity projects , any run configuration, partial lane flexibility)
- Illumina MiSeq (Standard v3 and v2 runs, Micro and Nano flow cell runs for pilot runs)
- Illumina iSeq (for shallow, pilot runs)
- PacBio SEQUEL IIe (Hi-Fi long reads for de novo genome assembly and isoform analysis, etc.)
- Oxford NanoPore PromethION-24 (Ultra-long reads for de novo genome assembly of high-throughput projects)
- Oxford NanoPore MinION (Ultra-long reads for de novo genome assembly of small genomes or pilot runs)

Library Construction Services for Major Applications (in conjunction with GE/GT core):

- Illumina (shotgun-genome, WGBS, RRBS, 16S metagenomics, COVID-seq, RNA-seq, Exome-seq)
- PacBio (large-insert genome, IsoSeq, Amplicon-seq, full-length 16S)
- Oxford NanoPore (large-insert genome, full-length cDNA, Direct RNA-seq, full-length 16S, COVID-seq)
- 10X Genomics (Single-cell: RNA-Seq, ATACseq, VDJ)

Sample/Library QC

- QUBIT
- Covaris S220, E220
- qPCR
- TapeStation
- SAGE ELF

Project Consultation
TapeStation
Qubit Assays
Assisted use of Covaris for sample fragmentation
Fragment Selection (ELF-based)
Sample/library clean-up
Load only sequencing instrument runs
Library pooling and pool quantitation

Please note that our library construction and sequencing services do not include data processing. Visit ICBR Bioinformatics to request Data Analysis services.

CORE NEWS

UF Investigators Identify Mechanism of Drug Resistance in Non-Small Cell Lung Cancer

UF Investigators Identify Mechanism of Drug Resistance in Non-Small Cell Lung Cancer Steven J Madore, PhD (ICBR Associate Director for Science) Summarized from J. Clin. Inv. 2025;135(6); e185149 Non-small cell lung cancer (NSCLC) is a leading cause of cancer-related deaths worldwide. Despite a combination of surgery and chemotherapy, survival rate remains low due to cancer cell metastasis and drug resistance. Mutations in the oncogene KRAS are frequently found in human cancers, including up to ...

Stars Align for Science: University of Florida and NSF NRAO Forge Path for Discovery

Stars Align for Science: University of Florida and NSF NRAO Forge Path for Discovery The U.S. National Science Foundation National Radio Astronomy Observatory (NSF NRAO) and the University of Florida (UF) have announced a dynamic new partnership to advance science education and public outreach at a future Next Generation Very Large Array (ngVLA) antenna site in Florida. This collaboration, signed with a Memorandum of Understanding (MOU) at the debut of the ngVLA prototype antenna in New Mexico l...

New Instruments in the ICBR NGS Shared Resource Will Lower DNA Sequencing Costs for UF Investigators

New Instruments in the ICBR NGS Shared Resource Will Lower DNA Sequencing Costs for UF Investigators Steven Madore, PhD, ICBR Associate Director for Science The mission of the ICBR Next Generation DNA Sequencing shared resource (NGS-SR) is to provide cost-effective access to cutting edge DNA sequencing technologies for the UF research community. The NGS-SR offers library preparation and sequencing instrumentation for both short (300-500 base pairs fragments) and long read (5,000 to 25,000 base p...

Florida Trend: Seeding Exploration

Seeding Exploration Twelve years to get 12 grams. Twelve years of research and planning, of conducting experiments with simulated material, of traveling to the world’s most inhospitable environments. Twelve years of writing proposals and being told no. University of Florida research scientists Rob Ferl and Anna-Lisa Paul weren’t asking NASA for just 12 grams oflunar regolith – basically dust and dirt from the moon – but that was the payoff in 2022 for their perseverance. ...

Sony ID7000 Spectral Cytometer at UF ICBR

Sony ID7000 Spectral Cytometer at UF ICBR The Cytometry & Optical Microscopy core at UF ICBR has recently acquired the state-of-the-art Sony ID7000 Spectral Cytometer, located at McKnight Brain Institute (MBI) room L3-160. This newest addition to the facility offers unparalleled capabilities for researchers, significantly expanding their experimental possibilities. The Sony ID7000 features an impressive configuration of five lasers and 144 detectors, enabling experiments utilizing over 40 fl...

ICBR Billing Schedule Update: Key Dates for Fiscal Year End 2025

Please note: Due to the Fiscal Year End, the last invoices to process for FY2025 will be created on Monday, June 9th. ICBR’s upcoming billing schedule will be altered as follows: 6/9/2025 – Last invoices generated in fiscal year 2025 6/16/2025 at noon – Deadline to submit disputes for FY2025 payment. 6/30/2025 – Invoices will begin to be created for FY2026 If you have expiring funds, please work with your core laboratory contact to make sure your project is completed and billed before the 06/...

Negative Effects of Common Weed Killer on Manatee Immune System

Negative Effects of Common Weed Killer on Manatee Immune System by Steven Madore, PhD; (Summarized from www.sciencedirect.com) Glyphosate is the herbicide used globally for agricultural and non-agricultural purposes to treat a broad spectrum of weeds. In Florida, this chemical is used in agriculture and to control the growth of invasive aquatic plants in lakes and rivers. It is also used widely for non-agricultural purposes on sidewalks, parks, and private homes to kill weeds. Glyphosate can be ...

UF ICBR Closed for Martin Luther King, Jr. Day

UF ICBR Closed for Martin Luther King, Jr. Day UF ICBR will be closed on Monday, January 20h in observation of Martin Luther King, Jr. Day. Normal hours will resume on Tuesday, January 21st. No packages will be accepted during this time! ...

CORE INSTRUMENTS