NextGen DNA Sequencing

Overview

CORE OVERVIEW

Welcome to the UF ICBR NextGen DNA Sequencing core facility. The ICBR-NGS core provides researchers with quality, massively parallel, high-throughput sequencing data using the most current instrumentation, at a reasonable cost. We currently support the most popular platforms: Illumina NovaSeq6000 and MiSeq and the Pacific Biosciences SEQUEL IIe system. Together, these instruments cover a broad range of powerful applications. Our free consultation services aim to help researchers navigate through the complex matrix of experimental options represented by the available sequencing technologies. Some of these important considerations include: read length, error rate, predominant type of error, data output/run, speed, cost, etc. The ever-broadening range of applications include (but are not limited to) de novo sequencing of whole genomes, targeted sequencing, transcriptome sequencing (RNA-Seq), chromatin immunoprecipitation (ChIP) sequencing, methylation analysis and metagenomics.

Our sample prep and library construction services are offered through both the NGS and the GeneExpression/Genotyping Cores. Please contact us if the service of your interest is not listed. We strive to support most common applications.

SERVICES OVERVIEW

Don’t see the service you are looking for? Visit UF | ICBR Gene Expression & Genotyping for more options.

Sequencing Services

Library Construction Services

General Services

NovaSeq 6000 Sequencing

Request services for: Sequencing formats (S4 2×150, S4 2×100, S2 2×150, S2 2×100, S2 2×50, S1 2×150, S1 2×100, S1 2×50, SP 2×250, SP 2×150, SP 2×50)

MiSeq Illumina Sequencing

Request services for: Sequencing formats (2x300v3, 2x250v2, 2x150v2, 2x75v3, 1x300v2, 1x150v3, 1x50v2)

PacBio SEQUEL IIe Sequencing

Sequencing formats (10 to 30 hour movies). LR SMRT cells
Current chemistry (early 2021): SMRT Link 10.0
Reads per SMRT cell run: 4-5 million reads

Illumina Standard DNA-Seq
Illumina PCR-free DNA-Seq
Illumina Whole Genome Bisulfite-converted WGBS)
Illumina Reduced Representation Bisulfite converted (RRBS)
Illumina Ultra-low input DNA-Seq
Illumina ATAC-seq
Illumina COVID-Seq
10X Genomics ATAC-Seq
PacBio Long-insert DNA-Seq for de novo assemblies
PacBio Barcoded bacterial DNA for multiplex sequencing
PacBio Barcoded amplicon-Seq (up to 10 Kb)
PacBio IsoSeq for transcriptome/isoform analysis
PacBio Full-length 16S metagenomics

Project Consultation
Bioanalyzer, TapeStation
Qubit Assays
Assisted use of Covaris for sample fragmentation
Fragment Selection (ELF-based)
Sample/library clean-up
Load only sequencing instrument runs
Library pooling and pool quantitation

Please note that our library construction and sequencing services do not include data processing. Visit ICBR Bioinformatics to request Data Analysis services.

CORE NEWS

ICBR Closed Monday for the 4th of July

ICBR Closed Monday for the 4th of July ICBR will be closed this Monday (7/4) in observation of the 4th of July We will not be accepting packages or samples on Monday. Our normal business hours will resume on Tuesday (7/5). Enjoy the the long weekend and celebrate responsibly! Please refer to our staff directory for additional details. ...

Plants can grow in Moon dirt — here’s why knowing that is so important

Plants can grow in Moon dirt — here’s why knowing that is so important The Hill Anna-Lisa Paul and Robert Ferl Humans are once again headed to the Moon. This return to the Moon means we will, for the first time, witness humans not only visiting, but living and working on the Moon. We will witness our civilization literally moving off the surface of the Earth. The lunar landings of 1969 through 1972 were short stays. The demands of getting to and returning from the Moon with the technology of tha...

ICBR attends AGBT 2022 General Meeting!

This June, ICBR attended the Advances in Genome Biology and Technology (AGBT) General Meeting in Orlando, the preeminent genome science and technology conference for top global researchers, leaders and innovators. AGBT delivers a premier experience where heads of labs, institutions, businesses, financial analysts and other high-level stakeholders come together to advance the field and drive game-changing innovation. Significant scientific advances are announced and showcased, science and industr...

In 27,000-acre Preserve, Scientists Begin ‘Age of Discovery’

In 27,000-acre Preserve, Scientists Begin ‘Age of Discovery’ UF Explore Cindy Spence Brent Sellers never tires of visitors’ reaction when he gives them a tour of the DeLuca Preserve, a 27,000-acre gem donated to the University of Florida in 2020. His four-wheel drive Silverado 2500 is like a time machine as he opens a gate and steers it straight into the past, into a Florida known today mostly in history book pictures. It’s wild and unpopulated, not the Florida most people see every day, and he ...

UF researchers evaluate how to improve dignity for seriously ill patients

UF researchers evaluate how to improve dignity for seriously ill patients UF Health Diana Tonnessen Hearing that you have a life-threatening illness can be physically and emotionally challenging. And while great strides have been made in helping people with a terminal illness manage their physical symptoms toward the end of their lives, helping them cope with the accompanying emotional distress is another story. “There is a real need for therapeutic interventions that improve the ability of peop...

Sea turtle conservation gets boost from new DNA detection method

Sea turtle conservation gets boost from new DNA detection method UF News Natalie van Hoose DNA “fingerprints” left behind by sea turtles offer scientists a simple, powerful way of tracking the health and whereabouts of these endangered animals, a key step forward in their conservation. A study led by University of Florida researchers is the first to sequence environmental DNA, or eDNA, from sea turtles — genetic material shed as they travel over beaches and in water. The research project is also...

Important Dates for Fiscal Year End

Please note: Due to the Fiscal Year End, the last invoices to process for FY2022 will be created on Monday, June 13th. Due to Fiscal Year End, ICBR’s upcoming billing schedule will be altered as follows: 6/13/2022 – Last invoices generated in fiscal year 2022 6/23/2022 at noon – Deadline to submit disputes for FY2022 payment. 7/5/2022 – Invoices will begin to be created for FY2023 If you have expiring funds, please work with your core laboratory contact to make sure your project is completed ...

A first: Scientists grow plants in soil from the Moon

A first: Scientists grow plants in soil from the Moon from UF/IFAS by Samantha Murray Scientists have grown plants in soil from the Moon, a first in human history and a milestone in lunar and space exploration. In a new paper published in the journal “Communications Biology,” University of Florida researchers showed that plants can successfully sprout and grow in lunar soil. Their study also investigated how plants respond biologically to the Moon’s soil, also known as lunar regolith, which is r...

CORE INSTRUMENTS