NextGen DNA Sequencing

ICBR-NextGenSeq@ad.ufl.edu   ///  (352) 273-8050   ///    RRID:SCR_019152
High-throughput Sequencing Services, Library Construction, Fragmentation and More

Overview

CORE OVERVIEW

Welcome to the UF ICBR NextGen DNA Sequencing core facility. ICBR NextGen DNA Sequencing provides quality, massively parallel, high-throughput sequencing data. We currently support the following platforms: PacBio (SEQUEL IIe), Illumina (NovaSeq6000, MiSeq), Oxford NanoPore (PromethION-24, MinION), and 10X Genomics Chromium,. These instruments allow for a broad range of sequencing applications for biological research. Customers are encouraged to consult with core staff for the purpose of tailoring data acquisition to specific research questions.

Our sample prep and library construction services are offered through both the NGS and the GeneExpression/Genotyping Cores. Please contact us if the service of your interest is not listed. We strive to support most common applications.

SERVICES OVERVIEW

Don’t see the service you are looking for? Visit UF | ICBR Gene Expression & Genotyping for more options.

Sequencing Services

Library Construction Services

Sample/Library QC

General Services

Sequencing

    • Illumina NovaSeq6000 (high-throughput and high-plexicity projects , any run configuration, partial lane flexibility)
    • Illumina MiSeq (Standard v3 and v2 runs, Micro and Nano flow cell runs for pilot runs)
    • Illumina iSeq (for shallow, pilot runs)
    • PacBio SEQUEL IIe (Hi-Fi long reads for de novo genome assembly and isoform analysis, etc.)
    • Oxford NanoPore PromethION-24 (Ultra-long reads for de novo genome assembly of high-throughput projects)
    • Oxford NanoPore MinION (Ultra-long reads for de novo genome assembly of small genomes or pilot runs)

Library Construction Services for Major Applications (in conjunction with GE/GT core):

    • Illumina (shotgun-genome, WGBS, RRBS, 16S metagenomics, COVID-seq, RNA-seq, Exome-seq)
    • PacBio (large-insert genome, IsoSeq, Amplicon-seq, full-length 16S
    • Oxford NanoPore (large-insert genome, full-length cDNA, Direct RNA-seq, full-length 16S, COVID-seq)
    • 10X Genomics (Single-cell: RNA-Seq, ATACseq, VDJ)

 

Sample/Library QC

    • QUBIT
    • Covaris S220, E220
    • qPCR
    • TapeStation
    • SAGE ELF
  • Project Consultation
  • Bioanalyzer, TapeStation
  • Qubit Assays
  • Assisted use of Covaris for sample fragmentation
  • Fragment Selection (ELF-based)
  • Sample/library clean-up
  • Load only sequencing instrument runs
  • Library pooling and pool quantitation

Please note that our library construction and sequencing services do not include data processing. Visit ICBR Bioinformatics to request Data Analysis services.

CORE NEWS

Important Dates for Fiscal Year End Important Dates for Fiscal Year End
Important Dates for Fiscal Year End
Please note: Due to the Fiscal Year End, the last invoices to process for FY2022 will be created on Monday, June 13th.  Due to Fiscal Year End, ICBR’s upcoming billing schedule will be altered as follows: 12/12/2022 – Last invoices generated in 2022 12/19/2022 at noon – Deadline to submit disputes for 2022 payment. If you have expiring funds, please work with your core laboratory contact to make sure your project is completed and billed before the 6/13/2022 invoices are posted. ...
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Important Dates for Fiscal Year End
ICBR Closed over Thanksgiving Holiday ICBR Closed over Thanksgiving Holiday
ICBR Closed over Thanksgiving Holiday
ICBR will be closed Thursday (November 24th) and Friday (November 25th) for the Thanksgiving holiday. We here at ICBR are thankful for you, and hope you enjoy the holiday!   Normal business hours will resume on Monday, November 28th.     ...
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ICBR Closed over Thanksgiving Holiday
Scientific expedition to study human impact on Florida Everglades Scientific expedition to study human impact on Florida Everglades
Scientific expedition to study human impact on Florida Everglades
Scientific expedition to study human impact on Florida Everglades A team of five that includes a female University of Florida professor as its lead scientist will embark Thursday on a dangerous trek across the Florida Everglades to assess the impact of humans on the world’s largest subtropical wilderness. The group will retrace an 1897 canoe journey that was first completed by explorer and scientist Hugh de Laussat Willoughby. Tracie Baker, an associate professor of environmental and global h...
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Scientific expedition to study human impact on Florida Everglades
Big Changes for ICBR Electron Microscopy Core in 2023 Big Changes for ICBR Electron Microscopy Core in 2023
Big Changes for ICBR Electron Microscopy Core in 2023
We are excited to announce that our EM core is going to be relocated entirely in CGRC. The move should not only offer our users additional convenience with a singular location and increased proximity to our peer cores, but the renovation will provide our users with a revamped lab space that will expand the capabilities and offerings of our EM team. We are very excited about the next steps, but there is still a lot of work left to do. EM is currently doing their best to facilitate this move while...
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Big Changes for ICBR Electron Microscopy Core in 2023
ICBR Announces Special Pilot Grant Opportunity ICBR Announces Special Pilot Grant Opportunity
ICBR Announces Special Pilot Grant Opportunity
DEADLINE EXTENDED: ICBR Announces Special Pilot Grant Opportunity The mission of ICBR is to champion and enable scientific discovery and innovation throughout the University of Florida research community by providing access to world-class biotechnology expertise and instrumentation. To introduce the UF research community to the use of Oxford Nanopore Technology PromethION nanopore DNA sequencing, the ICBR is requesting pilot project proposals utilizing this technology for funding consideration. ...
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ICBR Announces Special Pilot Grant Opportunity
Repeat performance: UF again ranked No. 5 among public universities by U.S. News & World Report Repeat performance: UF again ranked No. 5 among public universities by U.S. News & World Report
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Repeat performance: UF again ranked No. 5 among public universities by U.S. News & World Report UF News For the second year in a row, the University of Florida is listed as one of the top five public research universities in the nation, according to the 2023 Best Colleges rankings released today by U.S. News & World Report. The No. 5 recognition reaffirms UF’s status as an academic powerhouse in research, innovation, educational excellence, student outcomes and technology transfer. ...
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Repeat performance: UF again ranked No. 5 among public universities by U.S. News & World Report
Study uncovers differences in saliva bacteria of students with recent suicidal thoughts Study uncovers differences in saliva bacteria of students with recent suicidal thoughts
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Study uncovers differences in saliva bacteria of students with recent suicidal thoughts Samantha Murray A new University of Florida study has found that bacteria in the saliva of college students who reported recent thoughts of suicide differed in significant ways from those found in students who had not experienced recent suicidal thinking. While there is a growing body of research on mental health and the human microbiome, this is the first study to look at bacterial differences in the sa...
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Ant queens control insulin to boost lifespan, reproduction Eric Hamilton In most of the animal world, there’s a sad trade-off to make: The more babies you have, the shorter you live. But ants buck the system. The queens — the only individuals in a nest that reproduce — also live five, 10, up to 30 times longer than their genetically identical worker sisters. How do they pull off what the rest of animalkind cannot? A new study from University of Florida biologist Hua Yan and his collea...
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Ant queens control insulin to boost lifespan, reproduction

CORE INSTRUMENTS

NextGen DNA Sequencing

CORE PERSONNEL

NameTelephoneE-mailRoom

UF | ICBR Administration

Administration
Name/Title/DepartmentPhoneEmailLocationAdministration

Dr. Anna-Lisa Paul
Director, UF | ICBR

Administration

(352) 273-8029alp@ufl.edu160 CGRCAdministration

Dr. Steven Madore
Associate Director of Science, UF | ICBR
Administration

(352) 273-8038smadore@ufl.edu156 CGRCAdministration

Heather Voet
Associate Director of Administration
Administration

(352) 273-9461hvoet@ufl.edu161 CGRCAdministration



Administration

UF | ICBR Business Services

Business Services
Name/Title/DepartmentPhoneEmailLocationBusiness Services
Heather Voet
Associate Director of Administration
Business Services
(352) 273-9461hvoet@ufl.edu161 CGRCBusiness Services
Tim Hillmann
Finance Manager
Business Services
(352) 273-8027tjh4501@ufl.edu163 CGRCBusiness Services
Erica Boyd
Human Resources Generalist III
Business Services
(352) 273-8271ericalboyd@ufl.edu158 CGRCBusiness Services
Tina Hasko
Business Administrative Specialist III
Business Services
(352) 273-8048thasko@ufl.edu154 CGRCBusiness Services
Mackenzie Madore
Administrative Specialist II
Business Services
(352) 273-8065mmadore@ufl.edu157 CGRCBusiness Services
Tensi Krpan
Administrative Specialist II
Business Services
(352) 273-8026tenser9@ufl.edu181 CGRCBusiness Services

Kimberly Backer-Kelley
Core Research Facility Technician II
Administration

(352) 273-5341klbk@ufl.edu185 CGRCBusiness Services



Business Services

UF | ICBR Cyberinfrastructure

Cyberinfrastructure
Name/Title/DepartmentPhoneEmailLocationCyberinfrastructure
Ben Thebaut
IT Analyst II
Cyberinfrastructure
(352) 273-6814ben.thebaut@ufl.edu188 CGRCCyberinfrastructure
Cameron Smith
Systems Administrator/Programmer II
Cyberinfrastructure 
(352) 273-9651pc.smith@ufl.edu188 CGRCCyberinfrastructure
Manuel Luciano
Systems Administrator/Programmer II
Cyberinfrastructure 
(352) 273-8054manuel.luciano@ufl.edu188 CGRCCyberinfrastructure
Daniel Tarlini 
IT Analyst I
Cyberinfrastructure
(352) 273-8170tarlinid@ufl.edu188 CGRCCyberinfrastructure



Cyberinfrastructure

UF | ICBR Communications

Communications
Name/Title/DepartmentPhoneEmailLocationCommunications
Chris Dillon
Communications Section Director
Communications
(352) 273-8168csdillon@ufl.edu162 CGRCCommunications



Communications

UF | ICBR Bioinformatics

Email Core

Bioinformatics
Name/Title/DepartmentPhoneEmailLocationBioinformatics
Dr. Alberto Riva
Scientific Director
Bioinformatics
(352) 294-5475ariva@ufl.edu191 CGRCBioinformatics
Dr. Fahong Yu
Scientist
Bioinformatics
(352) 273-8064fyu@ufl.edu191 CGRCBioinformatics
Dr. Tongjun Gu
Assistant Scientist
Bioinformatics
(352) 273-8058tgu@ufl.edu191 CGRCBioinformatics



Bioinformatics

UF | ICBR Cytometry

Email Core

Cytometry
Name/Title/DepartmentPhoneEmailLocationCytometry
Dr. Mariza Miranda
Scientific Director
Cytometry
(352) 273-6032marizamiranda@ufl.edu292 CGRCCytometry
Doug Smith
Senior Laboratory Technician  
Cytometry
(352) 392-1745dugsmith@ufl.eduLG-164Cytometry
Alexander Linares
Core Research Facility Technician III
Cytometry
(352) 273-6032a.linares@ufl.eduCGRC 292Cytometry



Cytometry

UF | ICBR Electron Microscopy

Email Core

Electron Microscopy
Name/Title/DepartmentPhoneEmailLocationElectron Microscopy
Paul R. Chipman
Scientific Director
Electron Microscopy
(352) 294-1790pchipman@ufl.edu1012 MCSElectron Microscopy
Karen Kelley
Core Research Facility Specialist III
Electron Microscopy
(352) 273-8547vau@ufl.edu1012 MCS / 185 CGRCElectron Microscopy
Rudy Alvarado
Core Research Facility Specialist I
Electron Microscopy
(352) 846-0251ralvaradojr@ufl.edu1012 MCSElectron Microscopy
Mary Gragg
OPS Lab Technician
Electron Microscopy
(352) 846-0251mary.gragg@ufl.edu1012 MCSElectron Microscopy



Electron Microscopy

UF | ICBR Gene Expression & Genotyping

Email Core

Gene Expression & Genotyping
Name/Title/DepartmentPhoneEmailLocationGene Expression & Genotyping
Dr. Yanping Zhang
Scientific Director
Gene Expression & Genotyping
(352) 273-8043yanp@ufl.edu178F CGRCGene Expression & Genotyping
Brandon Pate
Core Research Facility Specialist II
Gene Expression & Genotyping
(352) 273-8036Bpate1@ufl.edu178 CGRCGene Expression & Genotyping
Mark Renshaw
Core Research Facility Specialist II
Gene Expression & Genotyping
(352) 273-8036marenshaw@ufl.edu178 CGRCGene Expression & Genotyping
Yasemin Can
Core Research Facility Technician III
Gene Expression & Genotyping
(352) 273-8036ycan@ufl.edu178 CGRCGene Expression & Genotyping
Sewande Mudiyanselage
Student Assistant
Gene Expression & Genotyping
(352) 273-8036s.mudiyanselage@ufl.edu178 CGRCGene Expression & Genotyping
Paul Fernandez
Student Assistant
Gene Expression & Genotyping
(352) 273-8036fernandezp@ufl.edu178 CGRCGene Expression & Genotyping
Ashlyn Lake
Student Assistant
Gene Expression & Genotyping
(352) 273-8036ashlynlake@ufl.edu178 CGRCGene Expression & Genotyping



Gene Expression & Genotyping

UF | ICBR Monoclonal Antibody

Email Core

Monoclonal Antibody
Name/Title/DepartmentPhoneEmailLocationMonoclonal Antibody
Dr. Angel Sampson
Scientific Director
Monoclonal Antibody
(352) 273-8039angel.sampson@ufl.edu175 CGRCMonoclonal Antibody
Shadi Bootorabi
Senior Biological Scientist
Monoclonal Antibody
(352) 273-8041shb@ufl.edu171A CGRCMonoclonal Antibody
Katrina Witas
Core Research Facility Technician III
Monoclonal Antibody
(352) 273-8040kaekelly@ufl.edu171 CGRCMonoclonal Antibody
Lourdes Guallpa
Student Assistant
Monoclonal Antibody
(352) 273-8040lguallpa@ufl.edu171 CGRCMonoclonal Antibody



Monoclonal Antibody

UF | ICBR NextGen DNA Sequencing

Email Core

NextGen DNA Sequencing
Name/Title/DepartmentPhoneEmailLocationNextGen DNA Sequencing
Dr. David Moraga
Scientific Director
NextGen DNA Sequencing
(352) 273-8053moraga@ufl.edu178J CGRCNextGen DNA Sequencing
Dr. Diansy Zincke
Core Research Facility Specialist III
NextGen DNA Sequencing
(352) 273-8050dzincke@ufl.edu178 CGRCNextGen DNA Sequencing
Dr. Liming Zhao
Core Research Facility Specialist II
NextGen DNA Sequencing
(352) 273-8050lmzhao@ufl.edu178 CGRCNextGen DNA Sequencing
Xiao Hui Zhou
Core Research Facility Technician III
NextGen DNA Sequencing
(352) 273-8050zxh@ufl.edu178 CGRCNextGen DNA Sequencing
Benjamin Hoffman
Core Research Facility Technician III
NextGen DNA Sequencing
(352) 273-8050semplar@ufl.edu178 CGRCNextGen DNA Sequencing
Daina Elsayed
OPS Lab Technican
NextGen DNA Sequencing
(352) 273-8050relsayed@ufl.edu178 CGRCNextGen DNA Sequencing



NextGen DNA Sequencing

UF | ICBR Proteomics & Mass Spectrometry

Email Core

Proteomics & Mass Spectrometry
Name/Title/DepartmentPhoneEmailLocationProteomics & Mass Spectrometry
Dr. Jin Koh
Scientific Director
Proteomics & Mass Spectrometry
(352) 273-8059jinkoh@ufl.edu168A CGRCProteomics & Mass Spectrometry
Ran Zheng
Core Research Facility Specialist II
Proteomics & Mass Spectrometry
(352) 273-8061ranzheng@ufl.edu169 CGRCProteomics & Mass Spectrometry
Ning Zhu
Core Research Facility Specialist II
Proteomics & Mass Spectrometry
(352) 273-8067nzhu@ufl.edu169 CGRCProteomics & Mass Spectrometry
Fanchao Zhu
Core Research Facility Specialist I
Proteomics & Mass Spectrometry
(352) 273-8060fzhu9482@ufl.edu169 CGRCProteomics & Mass Spectrometry

Sequencing

    • Illumina NovaSeq6000 (high-throughput and high-plexicity projects , any run configuration, partial lane flexibility)
    • Illumina MiSeq (Standard v3 and v2 runs, Micro and Nano flow cell runs for pilot runs)
    • Illumina iSeq (for shallow, pilot runs)
    • PacBio SEQUEL IIe (Hi-Fi long reads for de novo genome assembly and isoform analysis, etc.)
    • Oxford NanoPore PromethION-24 (Ultra-long reads for de novo genome assembly of high-throughput projects)
    • Oxford NanoPore MinION (Ultra-long reads for de novo genome assembly of small genomes or pilot runs)