NextGen DNA Sequencing

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NEXTGEN DNA SEQUENCING: Home | Staff & Contact | Instruments | Services | Getting Started | Sample Requirements | Core Resources

Overview

Welcome to the UF ICBR NextGen DNA Sequencing core facility. The ICBR-NGS core provides researchers with quality, massively parallel, high-throughput sequencing data using the most current instrumentation, at a reasonable cost. We currently support the most popular platforms: Illumina NextSeq500 and MiSeq, HiSeq3000 and the Pacific Biosciences SEQUEL system. Together, these instruments cover a broad range of powerful applications. Our free consultation services aim to help researchers navigate through the complex matrix of experimental options represented by the available sequencing technologies. Some of these important considerations include: read length, error rate, predominant type of error, data output/run, speed, cost, etc. The ever-broadening range of applications include (but are not limited to) de novo sequencing of whole genomes, targeted sequencing, transcriptome sequencing (RNA-Seq), chromatin immunoprecipitation (ChIP) sequencing, methylation analysis and metagenomics.

Our sample prep and library construction services are offered through both the NGS and the GeneExpression/Genotyping Cores. Please contact us if the service of your interest is not listed. We strive to support most common applications.

ICBR NEXTGEN DNA Sequencing Services

Don't see the service you are looking for? Visit ICBR Gene Expression & Genotyping for more options.

General Services

Library Construction Services

Sequencing Services

Project Consultation
Bioanalyzer, TapeStation
Qubit Assays
Assisted use of Covaris for sample fragmentation
Fragment Selection (ELF-based)
Sample/library clean-up
Load only sequencing instrument runs
Library pooling and pool quantitation

Illumina Standard DNA-Seq
Illumina PCR-free DNA-Seq
Illumina Whole Genome Bisulfite-converted WGBS)
Illumina Reduced Representation Bisulfite converted (RRBS)
Illumina Ultra-low input DNA-Seq
Illumina ATAC-seq
10X Genomics linked-reads
10X Genomics ATAC-Seq
PacBio Long-insert DNA-Seq for de novo assemblies
PacBio Barcoded bacterial DNA for multiplex sequencing
PacBio Barcoded amplicon-Seq (up to 10 Kb)
PacBio IsoSeq for transcriptome/isoform analysis
PacBio Full-length 16S metagenomics

MiSeq Illumina Sequencing

Request services for: Sequencing formats (2x300v3, 2x250v2, 2x150v2, 2x75v3, 1x300v2, 1x150v3, 1x50v2)

NextSeq500 Illumina Sequencing

Request services for: Sequencing formats (HIGH output: 2×150, 2×75, 1×150, 1×75. MID output: 2×150, 2×75, 1×150)

HiSeq3000 Illumina Sequencing

Request services for: Sequencing formats (2×100, 2×150, 2×75, 1×150, 1×50)

PacBio SEQUEL Sequencing

Sequencing formats (10-hour movies, 20-hour movies). LR v3 SMRT cells
Current chemistry (early 2019): v3. SMRT Link 6.0
Reads per SMRT cell run: 400k-600k reads

Please note that our library construction and sequencing services do not include data processing. Visit ICBR Bioinformatics to request Data Analysis services.