NextGen DNA Sequencing

Overview

CORE OVERVIEW

Welcome to the UF ICBR NextGen DNA Sequencing core facility. ICBR NextGen DNA Sequencing provides quality, massively parallel, high-throughput sequencing data. We currently support the following platforms: PacBio (SEQUEL IIe), Illumina (NovaSeq X Plus, MiSeq), Oxford NanoPore (PromethION-24, MinION Mk1c). These instruments allow for a broad range of sequencing applications for biological research. Customers are encouraged to consult with core staff for the purpose of tailoring data acquisition to specific research questions.

Our sample prep and library construction services are offered through both the NGS and the Gene Expression/Genotyping Cores. Please contact us if the service of your interest is not listed. We strive to support most common applications.

SERVICES OVERVIEW

Don’t see the service you are looking for? Visit UF | ICBR Gene Expression & Genotyping for more options.

Sequencing Services

Library Construction Services

Sample/Library QC

General Services

Sequencing

- Illumina NovaSeq X Plus (high-throughput and high-plexicity projects , any run configuration, partial lane flexibility)
- Illumina MiSeq (Standard v3 and v2 runs, Micro and Nano flow cell runs for pilot runs)
- Illumina iSeq (for shallow, pilot runs)
- PacBio SEQUEL IIe (Hi-Fi long reads for de novo genome assembly and isoform analysis, etc.)
- Oxford NanoPore PromethION-24 (Ultra-long reads for de novo genome assembly of high-throughput projects)
- Oxford NanoPore MinION (Ultra-long reads for de novo genome assembly of small genomes or pilot runs)

Library Construction Services for Major Applications (in conjunction with GE/GT core):

- Illumina (shotgun-genome, WGBS, RRBS, 16S metagenomics, COVID-seq, RNA-seq, Exome-seq)
- PacBio (large-insert genome, IsoSeq, Amplicon-seq, full-length 16S)
- Oxford NanoPore (large-insert genome, full-length cDNA, Direct RNA-seq, full-length 16S, COVID-seq)
- 10X Genomics (Single-cell: RNA-Seq, ATACseq, VDJ)

Sample/Library QC

- QUBIT
- Covaris S220, E220
- qPCR
- TapeStation
- SAGE ELF

Project Consultation
TapeStation
Qubit Assays
Assisted use of Covaris for sample fragmentation
Fragment Selection (ELF-based)
Sample/library clean-up
Load only sequencing instrument runs
Library pooling and pool quantitation

Please note that our library construction and sequencing services do not include data processing. Visit ICBR Bioinformatics to request Data Analysis services.

CORE NEWS

ICBR Enhances DNA Sequencing Capabilities with the Illumina NovaSeq X Plus

ICBR Enhances DNA Sequencing Capabilities with the Illumina NovaSeq X Plus The ICBR Next Generation DNA Sequencing Core is thrilled to announce the introduction of the Illumina NovaSeq X Plus DNA sequencing platform! This revolutionary system brings a multitude of advantages, including enhanced throughput, reduced run times, and uncompromised sequencing data quality, surpassing the capabilities of its predecessor, the NovaSeq6000. Perhaps one of the most remarkable features of the NovaSeq X Plus...

Signaling Molecules Help Plants Fight Microbes – ICBR Scientists Contribute to Groundbreaking Research

Signaling Molecules Help Plants Fight Microbes - ICBR Scientists Contribute to Groundbreaking Research

Signaling Molecules Help Plants Fight Microbes – ICBR Scientists Contribute to Groundbreaking Research Scientists from the University of Florida Interdisciplinary Center for Biotechnology Research (ICBR) have made a significant contribution to a study published in the journal Nature Communications that advances our understanding of plant immune response. The research study was led by Dr. Zhonglin Mou in the UF Molecular and Cell Sciences Department and Dr. Shweta Chhajed in the UF Departme...

Veterans Day: ICBR Closure on November 10th

Veterans Day: ICBR Closure on November 10th Please note that the ICBR will be closed on November 10th in observation of Veterans Day. Kindly be aware that we will not be able to accept any packages or samples during this time. We will resume regular operations on the following business day. Thank you for your understanding, and we hope you have a fantastic Veterans Day weekend! Please refer to our staff directory for additional details. ...

High School Students Dive into Biotechnology at the UF Interdisciplinary Center for Biotechnology Research

High School Students Dive into Biotech at the UF Interdisciplinary Center for Biotechnology Research Last week, high school students from Mount Dora Christian Academy visited the Interdisciplinary Center for Biotechnology Research (ICBR). During their visit, they explored the expansive world of biotechnology and they learned about the ICBR’s pivotal role in advancing discoveries in the life sciences at UF. “Hosting visits of young aspiring scientists like this allows students with an interes...

Homecoming: ICBR Closure on October 6th

Homecoming: ICBR Closure on October 6th Please note that the ICBR will be closed on October 6th for Homecoming. Kindly be aware that we will not be able to accept any packages or samples during this time. We will resume regular operations on the following business day. Thank you for your understanding, and we hope you have a fantastic homecoming weekend! Please refer to our staff directory for additional details. ...

Dr. David Moraga: 30 Years of Advancing Scientific Excellence at ICBR

Dr. David Moraga: 30 Years of Advancing Scientific Excellence at ICBR David Moraga, PhD, the NextGen Sequencing director at the ICBR, is retiring after 30 years of service. Since 1993, Dr. Moraga has played a vital role in advancing the center’s mission of providing cutting-edge scientific technology and research support services to the global scientific community. “Dr. David Moraga’s name has been almost synonymous with DNA sequencing for decades at ICBR and in the UF biotech communi...

Labor Day Notice: ICBR Closed on September 4th

Labor Day Notice: ICBR Closed on September 4th Please note that the ICBR will be closed on September 4th in observance of Labor Day. Kindly be aware that we will not be able to accept any packages or samples during this time. We will resume regular operations on the following business day. Please refer to our staff directory for additional details. ...

ICBR Closed Wednesday, August 30th for Tropical Storm Idalia

ICBR Closed Wednesday, August 30th for Tropical Storm Idalia Due to Tropical Storm Idalia, the ICBR will close beginning at noon on (8/29) through Wednesday (8/30). We will not be accepting packages or samples during this time. The university expects to make an announcement on Wednesday regarding the resumption of normal campus operations. Please visit www.ufl.edu for updates and information on storm preparation. Please refer to our staff directory for additional details. ...

CORE INSTRUMENTS