NextGen DNA Sequencing
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NEXTGEN DNA SEQUENCING: Home | Staff & Contact | Instruments | Services | Getting Started | Sample Requirements | Core Resources
Overview
Welcome to the UF ICBR NextGen DNA Sequencing core facility. The ICBR-NGS core provides researchers with quality, massively parallel, high-throughput sequencing data using the most current instrumentation, at a reasonable cost. We currently support the most popular platforms: Illumina NextSeq500 and MiSeq, HiSeq3000 and the Pacific Biosciences SEQUEL system. Together, these instruments cover a broad range of powerful applications. Our free consultation services aim to help researchers navigate through the complex matrix of experimental options represented by the available sequencing technologies. Some of these important considerations include: read length, error rate, predominant type of error, data output/run, speed, cost, etc. The ever-broadening range of applications include (but are not limited to) de novo sequencing of whole genomes, targeted sequencing, transcriptome sequencing (RNA-Seq), chromatin immunoprecipitation (ChIP) sequencing, methylation analysis and metagenomics.
Our sample prep and library construction services are offered through both the NGS and the GeneExpression/Genotyping Cores. Please contact us if the service of your interest is not listed. We strive to support most common applications.
ICBR NEXTGEN DNA Sequencing Services
Don't see the service you are looking for? Visit ICBR Gene Expression & Genotyping for more options.
Sequencing Services
Library Construction Services
General Services
NovaSeq 6000 Sequencing
- Request services for: Sequencing formats (S4 2×150, S4 2×100, S2 2×150, S2 2×100, S2 2×50, S1 2×150, S1 2×100, S1 2×50, SP 2×250, SP 2×150, SP 2×50)
MiSeq Illumina Sequencing
- Request services for: Sequencing formats (2x300v3, 2x250v2, 2x150v2, 2x75v3, 1x300v2, 1x150v3, 1x50v2)
NextSeq500 Illumina Sequencing
- Request services for: Sequencing formats (HIGH output: 2×150, 2×75, 1×150, 1×75. MID output: 2×150, 2×75, 1×150)
HiSeq3000 Illumina Sequencing
- Request services for: Sequencing formats (2×100, 2×150, 2×75, 1×150, 1×50)
PacBio SEQUEL Sequencing
- Sequencing formats (10-hour movies, 20-hour movies). LR v3 SMRT cells
- Current chemistry (early 2019): v3. SMRT Link 6.0
- Reads per SMRT cell run: 400k-600k reads
- Illumina Standard DNA-Seq
- Illumina PCR-free DNA-Seq
- Illumina Whole Genome Bisulfite-converted WGBS)
- Illumina Reduced Representation Bisulfite converted (RRBS)
- Illumina Ultra-low input DNA-Seq
- Illumina ATAC-seq
- 10X Genomics linked-reads
- 10X Genomics ATAC-Seq
- PacBio Long-insert DNA-Seq for de novo assemblies
- PacBio Barcoded bacterial DNA for multiplex sequencing
- PacBio Barcoded amplicon-Seq (up to 10 Kb)
- PacBio IsoSeq for transcriptome/isoform analysis
- PacBio Full-length 16S metagenomics
- Project Consultation
- Bioanalyzer, TapeStation
- Qubit Assays
- Assisted use of Covaris for sample fragmentation
- Fragment Selection (ELF-based)
- Sample/library clean-up
- Load only sequencing instrument runs
- Library pooling and pool quantitation
Please note that our library construction and sequencing services do not include data processing. Visit ICBR Bioinformatics to request Data Analysis services.
NextGen DNA Sequencing News
ICBR Faculty Director to Speak at 2019 Florida Genetics Symposium
ICBR Faculty Director to Speak at 2019 Florida Genetics Symposium
We are excited to announce that Sixue Chen, PhD will present at the 2019 Florida Genetics Symposium, presented by the University of Florida Genetics Institute. This two day event on November 4th and 5th will bring together some of the brightest minds in genetics.
Faculty Director of the ICBR Proteomics & Mass Spectrometry Core, Dr. Chen will present “Single Cell-Type Proteomics and Metabolomics: New Insights into Guard Cell Umminuty and CO2 Responses” on Monday, November 4th from 1:45pm ... Read More
The Beast is Here! ICBR Launches the NovaSeq 6000 DNA Sequencer
The Beast is Here! ICBR Launches the NovaSeq 6000 DNA Sequencer
Dr. Steven Madore, ICBR Associate Director for Science and UFHCC Associate Director for Shared Resources, next to the new NovaSeq6000 (“the Beast“) with ICBR NGS Core Scientific Director Dr. David Moraga.
The ICBR NextGen DNA Sequencing Core provides cutting edge instrumentation and technical expertise required to support campus-wide research activites. Established in 1987, the NGS Core has contributed to significant scientific discoveries on campus. For example, did you know that the first... Read More
The Luminex FLEXMAP 3D® System – A Multiplexed Analytical Platform for Novel Biomarker Discovery
The Luminex FLEXMAP 3D® System – A Multiplexed Analytical Platform for Novel Biomarker Discovery
Figure 1. Luminex bead architecture technology
ICBR Proteomics & Mass Spectrometry (PM) Core is now offering access to the Luminex FLEXMAP 3D® analytical platform for discovering and validation of protein biomarkers across a wide range of diseases. The FLEXMAP 3D® system combines differentially dyed fluorescent microsphere sets with an innovative instrument design to enable simultaneous measurement of the concentration of a large number of different analytes in a rapid multiplexed as... Read More
Dr. Anna-Lisa Paul receives NASA’s Exceptional Scientific Achievement Medal
Dr. Anna-Lisa Paul receives NASA's Exceptional Scientific Achievement Medal
Congratulations to UF’s Dr. Anna-Lisa Paul on receiving the NASA Exceptional Scientific Achievement Medal this past September! The prestigious award is reserved for individual efforts that are in line with NASA’s mission and have resulted in considerable scientific discovery or resulted in contributions of fundamental importance in their field or significantly improved the understanding of their field.
Dr. Paul is the Interim Director at UF ICBR and Research Professor in the UF Horticultural... Read More
Introducing Core Directors’ Open Consultation Meetings
Introducing Core Directors' Open Consultation Meetings
In a continued effort to provide accessible and specialized expertise to our users, UF ICBR is proud to announce our Core Directors’ Open Consultation Meetings. Each month we will be available for a two hour walk-in session to discuss the best approaches and strategies for using ICBR technologies and expertise to drive your research forward. Our first meeting will be on Monday, October 7th from 11:00am to 1:00pm in CGRC 189. No appointment is require nor do you have to be a current ICBR user t... Read More
The NovaSeq 6000 has arrived at UF ICBR!
The NovaSeq 6000 has arrived at UF ICBR!
After much anticipation, the Illumina NovaSeq 6000 has arrived at UF ICBR. Users can expect access later this fall.
The NovaSeq 6000 Sequencing System unleashes groundbreaking innovations that leverage our proven technology. Now you can get scalable throughput and flexibility for virtually any sequencing method, genome, and scale of project.
The NovaSeq will be housed in our NextGen DNA Sequencing core. Stay tuned for more information and events this fall.
For more information on the instrument... Read More
ICBR Bioinformatics Contributes to Landmark UFHCC Publication
ICBR Bioinformatics Contributes to Landmark UFHCC Publication
The ICBR Bioinformatics Core contributed to a high-profile paper from the cancer research laboratory of UFHCC Director Dr. Jonathan Licht, recently published in Cancer Discovery.
The paper, entitled “A Mutation in Histone H2B Represents a New Class of Oncogenic Driver”, describes how mutations in core histones disrupt nucleosome stability, leading to disregulation of gene expression and acting as a novel oncogenic mechanism. As part of this study the investigators used a technique ca... Read More
Help ICBR Grow in Ten Quick Questions!
Help ICBR Grow in Ten Quick Questions!
We want to hear about your experience working with UF ICBR.
What do you like?
How can we improve?
We want to hear from you. For your convenience, we kept our survey to ten quick questions. This is your opportunity to help us continue our quest to become the best resource on campus.
Click Here to Participate in our Survey
... Read More
Instruments
Staff
| Name | Title | Telephone | Room | |
|---|---|---|---|---|
| David Moraga, Ph.D. | NextGen DNA Sequencing Scientific Director | (352) 273-8053 | moraga@ufl.edu | 178J CGRC |
| Wayra Navia Gine, M.S. | NextGen DNA Sequencing Core Research Specialist II | (352) 273-8068 | wnavia@ufl.edu | 178 CGRC |
| Nedka Panayotova, M.S. | NextGen DNA Sequencing Senior Biological Scientist | (352) 273-8050 | nedpan@ufl.edu | 178 CGRC |
| Xiao Hui Zhou, B.S. | NextGen DNA Sequencing Core Research Technician II | (352) 273-8050 | zxh@ufl.edu | 178 CGRC |
| Lydia Von Borstel, B.S. | NextGen DNA Sequencing Core Research Technician III | (352) 273-8050 | lvonborstel@ufl.edu | 178 CGRC |