NextGen DNA Sequencing

ICBR-NextGenSeq@ad.ufl.edu   ///  (352) 273-8050   ///    RRID:SCR_019152
High-throughput Sequencing Services, Library Construction, Fragmentation and More

Overview

CORE OVERVIEW

Welcome to the UF ICBR NextGen DNA Sequencing core facility. The ICBR-NGS core provides researchers with quality, massively parallel, high-throughput sequencing data using the most current instrumentation, at a reasonable cost. We currently support the most popular platforms: Illumina NovaSeq6000 and MiSeq and the Pacific Biosciences SEQUEL IIe system. Together, these instruments cover a broad range of powerful applications. Our free consultation services aim to help researchers navigate through the complex matrix of experimental options represented by the available sequencing technologies. Some of these important considerations include: read length, error rate, predominant type of error, data output/run, speed, cost, etc. The ever-broadening range of applications include (but are not limited to) de novo sequencing of whole genomes, targeted sequencing, transcriptome sequencing (RNA-Seq), chromatin immunoprecipitation (ChIP) sequencing, methylation analysis and metagenomics.

Our sample prep and library construction services are offered through both the NGS and the GeneExpression/Genotyping Cores. Please contact us if the service of your interest is not listed. We strive to support most common applications.

SERVICES OVERVIEW

Don’t see the service you are looking for? Visit UF | ICBR Gene Expression & Genotyping for more options.

Sequencing Services

Library Construction Services

General Services

NovaSeq 6000 Sequencing

  • Request services for: Sequencing formats (S4 2×150, S4 2×100, S2 2×150, S2 2×100, S2 2×50, S1 2×150, S1 2×100, S1 2×50, SP 2×250, SP 2×150, SP 2×50)

MiSeq Illumina Sequencing 

  • Request services for: Sequencing formats (2x300v3, 2x250v2, 2x150v2, 2x75v3, 1x300v2, 1x150v3, 1x50v2)

PacBio SEQUEL IIe Sequencing 

  • Sequencing formats (10 to 30 hour movies). LR SMRT cells
  • Current chemistry (early 2021): SMRT Link 10.0
  • Reads per SMRT cell run: 4-5 million reads
  • Illumina Standard DNA-Seq
  • Illumina PCR-free DNA-Seq
  • Illumina Whole Genome Bisulfite-converted WGBS)
  • Illumina Reduced Representation Bisulfite converted (RRBS)
  • Illumina Ultra-low input DNA-Seq
  • Illumina ATAC-seq
  • Illumina COVID-Seq
  • 10X Genomics ATAC-Seq
  • PacBio Long-insert DNA-Seq for de novo assemblies
  • PacBio Barcoded bacterial DNA for multiplex sequencing
  • PacBio Barcoded amplicon-Seq (up to 10 Kb)
  • PacBio IsoSeq for transcriptome/isoform analysis
  • PacBio Full-length 16S metagenomics
  • Project Consultation
  • Bioanalyzer, TapeStation
  • Qubit Assays
  • Assisted use of Covaris for sample fragmentation
  • Fragment Selection (ELF-based)
  • Sample/library clean-up
  • Load only sequencing instrument runs
  • Library pooling and pool quantitation

Please note that our library construction and sequencing services do not include data processing. Visit ICBR Bioinformatics to request Data Analysis services.

CORE NEWS

ICBR Closed Monday for the 4th of July ICBR Closed Monday for the 4th of July
ICBR Closed Monday for the 4th of July
ICBR Closed Monday for the 4th of July ICBR will be closed this Monday (7/4) in observation of the 4th of July   We will not be accepting packages or samples on Monday. Our normal business hours will resume on Tuesday (7/5). Enjoy the the long weekend and celebrate responsibly!   Please refer to our staff directory for additional details. ...
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ICBR Closed Monday for the 4th of July
Plants can grow in Moon dirt — here’s why knowing that is so important Plants can grow in Moon dirt — here’s why knowing that is so important
Plants can grow in Moon dirt — here’s why knowing that is so important
Plants can grow in Moon dirt — here’s why knowing that is so important The Hill Anna-Lisa Paul and Robert Ferl Humans are once again headed to the Moon. This return to the Moon means we will, for the first time, witness humans not only visiting, but living and working on the Moon. We will witness our civilization literally moving off the surface of the Earth. The lunar landings of 1969 through 1972 were short stays. The demands of getting to and returning from the Moon with the technology of tha...
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Plants can grow in Moon dirt — here’s why knowing that is so important
ICBR attends AGBT 2022 General Meeting! ICBR attends AGBT 2022 General Meeting!
ICBR attends AGBT 2022 General Meeting!
This June, ICBR attended the Advances in Genome Biology and Technology (AGBT) General Meeting in Orlando, the preeminent genome science and technology conference for top global researchers, leaders and innovators. AGBT delivers a premier experience where heads of labs, institutions, businesses, financial analysts and other high-level stakeholders come together to advance the field and drive game-changing innovation. Significant scientific advances are announced and showcased, science and industr...
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ICBR attends AGBT 2022 General Meeting!
In 27,000-acre Preserve, Scientists Begin ‘Age of Discovery’ In 27,000-acre Preserve, Scientists Begin ‘Age of Discovery’
In 27,000-acre Preserve, Scientists Begin ‘Age of Discovery’
In 27,000-acre Preserve, Scientists Begin ‘Age of Discovery’ UF Explore Cindy Spence Brent Sellers never tires of visitors’ reaction when he gives them a tour of the DeLuca Preserve, a 27,000-acre gem donated to the University of Florida in 2020. His four-wheel drive Silverado 2500 is like a time machine as he opens a gate and steers it straight into the past, into a Florida known today mostly in history book pictures. It’s wild and unpopulated, not the Florida most people see every day, and he ...
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In 27,000-acre Preserve, Scientists Begin ‘Age of Discovery’
UF researchers evaluate how to improve dignity for seriously ill patients UF researchers evaluate how to improve dignity for seriously ill patients
UF researchers evaluate how to improve dignity for seriously ill patients
UF researchers evaluate how to improve dignity for seriously ill patients UF Health Diana Tonnessen Hearing that you have a life-threatening illness can be physically and emotionally challenging. And while great strides have been made in helping people with a terminal illness manage their physical symptoms toward the end of their lives, helping them cope with the accompanying emotional distress is another story. “There is a real need for therapeutic interventions that improve the ability of peop...
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UF researchers evaluate how to improve dignity for seriously ill patients
Sea turtle conservation gets boost from new DNA detection method Sea turtle conservation gets boost from new DNA detection method
Sea turtle conservation gets boost from new DNA detection method
Sea turtle conservation gets boost from new DNA detection method UF News Natalie van Hoose DNA “fingerprints” left behind by sea turtles offer scientists a simple, powerful way of tracking the health and whereabouts of these endangered animals, a key step forward in their conservation. A study led by University of Florida researchers is the first to sequence environmental DNA, or eDNA, from sea turtles — genetic material shed as they travel over beaches and in water. The research project is also...
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Sea turtle conservation gets boost from new DNA detection method
Important Dates for Fiscal Year End Important Dates for Fiscal Year End
Important Dates for Fiscal Year End
Please note: Due to the Fiscal Year End, the last invoices to process for FY2022 will be created on Monday, June 13th.  Due to Fiscal Year End, ICBR’s upcoming billing schedule will be altered as follows: 6/13/2022 – Last invoices generated in fiscal year 2022 6/23/2022 at noon – Deadline to submit disputes for FY2022 payment. 7/5/2022 – Invoices will begin to be created for FY2023 If you have expiring funds, please work with your core laboratory contact to make sure your project is completed ...
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Important Dates for Fiscal Year End
A first: Scientists grow plants in soil from the Moon A first: Scientists grow plants in soil from the Moon
A first: Scientists grow plants in soil from the Moon
A first: Scientists grow plants in soil from the Moon from UF/IFAS by Samantha Murray Scientists have grown plants in soil from the Moon, a first in human history and a milestone in lunar and space exploration. In a new paper published in the journal “Communications Biology,” University of Florida researchers showed that plants can successfully sprout and grow in lunar soil. Their study also investigated how plants respond biologically to the Moon’s soil, also known as lunar regolith, which is r...
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A first: Scientists grow plants in soil from the Moon

CORE INSTRUMENTS

NextGen DNA Sequencing

CORE PERSONNEL

NameTelephoneE-mailRoom

UF | ICBR Administration

Administration
Name/Title/DepartmentPhoneEmailLocationAdministration

Dr. Anna-Lisa Paul
Director, UF | ICBR

Administration

(352) 273-8029alp@ufl.edu160 CGRCAdministration

Dr. Steven Madore
Associate Director of Science, UF | ICBR
Administration

(352) 273-8038smadore@ufl.edu156 CGRCAdministration

Heather Voet
Associate Director of Administration
Administration

(352) 273-9461hvoet@ufl.edu161 CGRCAdministration



Administration

UF | ICBR Business Services

Business Services
Name/Title/DepartmentPhoneEmailLocationBusiness Services
Heather Voet
Associate Director of Administration
Business Services
(352) 273-9461hvoet@ufl.edu161 CGRCBusiness Services
Tim Hillmann
Finance Manager
Business Services
(352) 273-8027tjh4501@ufl.edu163 CGRCBusiness Services
Erica Boyd
Human Resources Generalist II
Business Services
(352) 273-8271ericalboyd@ufl.edu158 CGRCBusiness Services
Tina Hasko
Business Administrative Specialist III
Business Services
(352) 273-8048thasko@ufl.edu154 CGRCBusiness Services
Mackenzie Madore
Administrative Specialist II
Business Services
(352) 273-8065mmadore@ufl.edu157 CGRCBusiness Services
Tensi Krpan
Administrative Specialist II
Business Services
(352) 273-8026tenser9@ufl.edu181 CGRCBusiness Services

Kimberly Backer-Kelley
Core Research Facility Technician II
Administration

(352) 273-5341klbk@ufl.edu185 CGRCBusiness Services



Business Services

UF | ICBR Cyberinfrastructure

Cyberinfrastructure
Name/Title/DepartmentPhoneEmailLocationCyberinfrastructure
Jeremy Jensen
Cyberinfrastructure Section Director
Cyberinfrastructure
(352) 273-9274jeremy.jensen@ufl.edu188 CGRCCyberinfrastructure
Ben Thebaut
IT Analyst II
Cyberinfrastructure
(352) 273-6814ben.thebaut@ufl.edu188 CGRCCyberinfrastructure
Cameron Smith
Systems Administrator/Programmer II
Cyberinfrastructure 
(352) 273-9651pc.smith@ufl.edu188 CGRCCyberinfrastructure
Manuel Luciano
Systems Administrator/Programmer II
Cyberinfrastructure 
188 CGRCCyberinfrastructure
Daniel Tarlini 
IT Analyst I
Cyberinfrastructure
(352) 273-8170tarlinid@ufl.edu188 CGRCCyberinfrastructure



Cyberinfrastructure

UF | ICBR Communications

Communications
Name/Title/DepartmentPhoneEmailLocationCommunications
Chris Dillon
Communications Section Director
Communications
(352) 273-8168csdillon@ufl.edu162 CGRCCommunications



Communications

UF | ICBR Bioinformatics

Email Core

Bioinformatics
Name/Title/DepartmentPhoneEmailLocationBioinformatics
Dr. Alberto Riva
Scientific Director
Bioinformatics
(352) 294-5475ariva@ufl.edu191 CGRCBioinformatics
Dr. Fahong Yu
Scientist
Bioinformatics
(352) 273-8064fyu@ufl.edu191 CGRCBioinformatics
Dr. Tongjun Gu
Assistant Scientist
Bioinformatics
(352) 273-8058tgu@ufl.edu191 CGRCBioinformatics



Bioinformatics

UF | ICBR Cytometry

Email Core

Cytometry
Name/Title/DepartmentPhoneEmailLocationCytometry
Dr. Patrick Kellish
Interim Scientific Director
Cytometry
(352) 273-8550pckellish@ufl.edu292 CGRCCytometry
Dr. Mariza Miranda
Core Research Specialist III
Cytometry
(352) 273-6032marizamiranda@ufl.edu292 CGRCCytometry
Doug Smith
Senior Laboratory Technician  
Cytometry
(352) 392-1745dugsmith@ufl.eduLG-164Cytometry
Alexander Linares
Core Research Facility Technician III
Cytometry
(352) 273-6032a.linares@ufl.eduCGRC 292Cytometry



Cytometry

UF | ICBR Electron Microscopy

Email Core

Electron Microscopy
Name/Title/DepartmentPhoneEmailLocationElectron Microscopy
Paul R. Chipman
Scientific Director
Electron Microscopy
(352) 294-1790pchipman@ufl.edu1012 MCSElectron Microscopy
Karen Kelley
Core Research Facility Specialist III
Electron Microscopy
(352) 273-8547vau@ufl.edu1012 MCS / 185 CGRCElectron Microscopy
Rudy Alvarado
Core Research Facility Technician III
Electron Microscopy
(352) 846-0251ralvaradojr@ufl.edu1012 MCSElectron Microscopy



Electron Microscopy

UF | ICBR Gene Expression & Genotyping

Email Core

Gene Expression & Genotyping
Name/Title/DepartmentPhoneEmailLocationGene Expression & Genotyping
Dr. Yanping Zhang
Scientific Director
Gene Expression & Genotyping
(352) 273-8043yanp@ufl.edu178F CGRCGene Expression & Genotyping
Brandon Pate
Core Research Facility Specialist II
Gene Expression & Genotyping
(352) 273-8036Bpate1@ufl.edu178 CGRCGene Expression & Genotyping
Dr. Junli Zhang
Core Research Facility Specialist II
Gene Expression & Genotyping
(352) 273-8036zjl1983@ufl.edu178 CGRCGene Expression & Genotyping
Yasemin Can
Core Research Facility Technician III
Gene Expression & Genotyping
(352) 273-8036ycan@ufl.edu178 CGRCGene Expression & Genotyping
Leyna Xoi
Core Research Facility Technician III
Gene Expression & Genotyping
(352) 273-8036leynaxoi@ufl.edu178 CGRCGene Expression & Genotyping
Sewande Mudiyanselage
Student Assistant
Gene Expression & Genotyping
(352) 273-8036s.mudiyanselage@ufl.edu178 CGRCGene Expression & Genotyping
Paul Fernandez
Student Assistant
Gene Expression & Genotyping
(352) 273-8036fernandezp@ufl.edu178 CGRCGene Expression & Genotyping
Ashlyn Lake
Student Assistant
Gene Expression & Genotyping
(352) 273-8036ashlynlake@ufl.edu178 CGRCGene Expression & Genotyping



Gene Expression & Genotyping

UF | ICBR Monoclonal Antibody

Email Core

Monoclonal Antibody
Name/Title/DepartmentPhoneEmailLocationMonoclonal Antibody
Dr. Angel Sampson
Scientific Director
Monoclonal Antibody
(352) 273-8039angel.sampson@ufl.edu175 CGRCMonoclonal Antibody
Shadi Bootorabi
Senior Biological Scientist
Monoclonal Antibody
(352) 273-8041shb@ufl.edu171A CGRCMonoclonal Antibody
Katrina Witas
Core Research Facility Technician III
Monoclonal Antibody
(352) 273-8040kaekelly@ufl.edu171 CGRCMonoclonal Antibody
Lourdes Guallpa
Student Assistant
Monoclonal Antibody
(352) 273-8040lguallpa@ufl.edu171 CGRCMonoclonal Antibody



Monoclonal Antibody

UF | ICBR NextGen DNA Sequencing

Email Core

NextGen DNA Sequencing
Name/Title/DepartmentPhoneEmailLocationNextGen DNA Sequencing
Dr. David Moraga
Scientific Director
NextGen DNA Sequencing
(352) 273-8053moraga@ufl.edu178J CGRCNextGen DNA Sequencing
Dr. Diansy Zincke
Core Research Facility Specialist II
NextGen DNA Sequencing
(352) 273-8050dzincke@ufl.edu178 CGRCNextGen DNA Sequencing
Dr. Liming Zhao
Core Research Facility Specialist II
NextGen DNA Sequencing
(352) 273-8050lmzhao@ufl.edu178 CGRCNextGen DNA Sequencing
Xiao Hui Zhou
Core Research Facility Technician III
NextGen DNA Sequencing
(352) 273-8050zxh@ufl.edu178 CGRCNextGen DNA Sequencing
Benjamin Hoffman
Core Research Facility Technician III
NextGen DNA Sequencing
(352) 273-8050semplar@ufl.edu178 CGRCNextGen DNA Sequencing
Iniya Thiyagarajan
Lab Assistant
NextGen DNA Sequencing
(352) 273-8050ithiyagarajan@ufl.edu178 CGRCNextGen DNA Sequencing



NextGen DNA Sequencing

UF | ICBR Proteomics & Mass Spectrometry

Email Core

Proteomics & Mass Spectrometry
Name/Title/DepartmentPhoneEmailLocationProteomics & Mass Spectrometry
Dr. Sixue Chen
Faculty Director
Proteomics & Mass Spectrometry
(352) 273-8057schen@ufl.edu169A CGRCProteomics & Mass Spectrometry
Dr. Jin Koh
Scientific Director
Proteomics & Mass Spectrometry
(352) 273-8059jinkoh@ufl.edu168A CGRCProteomics & Mass Spectrometry
Ran Zheng
Core Research Facility Specialist I
Proteomics & Mass Spectrometry
(352) 273-8061ranzheng@ufl.edu169 CGRCProteomics & Mass Spectrometry
Dr. Ning Zhu
Core Research Facility Specialist I
Proteomics & Mass Spectrometry
(352) 273-8067nzhu@ufl.edu169 CGRCProteomics & Mass Spectrometry
Fanchao Zhu
Core Research Facility Technician III
Proteomics & Mass Spectrometry
(352) 273-8060fzhu9482@ufl.edu169 CGRCProteomics & Mass Spectrometry