NextGen DNA Sequencing

Services & Fees
NEXTGEN DNA SEQUENCING:  Home   Staff & ContactInstruments  |  Services   |  Getting Started   | Sample Requirements  |  Core Resources

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Offered Services

10X Genomic Services

Request services for: a) Library construction (Genomic linked-read and Single-cell ATAC-Seq); b) QC of pre-constructed 10x libraries (Qubit and TapeStation); c) Pooling of pre-constructed 10x libraries and QC of the pool; d) Sequencing of 10x libraries (10x Single cell, 10x linked-read, 10x ATAC-Seq, and 10x CNV).

For single-cell (sc) RNAseq, sc-CNV and sc-Tcell V(D)J please see Gene expression and Genotyping core services.

Consultation

Schedule a consultation to discuss custom project needs and prepare a project outline with an ICBR staff member.

General Services

Request general services: Bioanalyzer, TapeStation, Qubit Assays, Assisted use of Covaris for sample fragmentation, Fragment Selection (ELF-based), Sample/library clean-up and Load only sequencing instrument runs, Library pooling and pool quantitation, Library construction only.

MiSeq Illumina Sequencing

Request services for: a) library construction (Standard DNA-Seq, Ultra-low input DNA-seq, PCR-free DNA-Seq, and Bisulfite-converted) with sequencing on the MiSeq; b) Sequencing of core-constructed libraries or customer-constructed libraries (i.e, any type of pre-constructed libraries). Sequencing formats (2x300v3, 2x250v2, 2x150v2, 2x75v3, 1x300v2, 1x150v3, 1x50v2).

For For single-cell (sc) RNAseq, sc-CNV, sc-Tcell V(D)J, Standard RNAseq, Exome capture library preparation, please see Gene Expression and Genotyping Core services.

NextSeq500 Illumina Sequencing

Request services for: a) library construction (Standard DNA-Seq, PCR-free DNA-Seq, and Bisulfite-converted) and sequencing on the NextSeq500; b) Sequencing of core-constructed libraries or customer-constructed libraries (i.e, any type of pre-constructed libraries). Sequencing formats (HIGH output: 2×150, 2×75, 1×150, 1×75. MID output: 2×150, 2×75, 1×150).

For For single-cell (sc) RNAseq, sc-CNV, sc-Tcell V(D)J, Standard RNAseq, Exome capture library preparation, please see Gene Expression and Genotyping Core services.

HiSeq3000 Illumina Sequencing

Request services for: a) library construction (Standard DNA-Seq, PCR-free DNA-Seq, and Bisulfite-converted) and sequencing on the HiSeq3000; b) Sequencing of core-constructed libraries or customer-constructed libraries (i.e, any type of pre-constructed libraries). Sequencing formats (2×100, 2×150, 2×75, 1×150, 1×50).

For For single-cell (sc) RNAseq, sc-CNV, sc-Tcell V(D)J, Standard RNAseq, Exome capture library preparation, please see Gene Expression and Genotyping Core services.

PacBio SEQUEL Sequencing

Request services for: a) Library construction (long-insert DNA-Seq, barcoded amplicon-Seq, and IsoSeq) and sequencing on the PacBio SEQUEL; b) Sequencing of core-constructed libraries or customer-constructed libraries (i.e, any type of pre-constructed libraries). Sequencing formats (10-hour movies, 20-hour movies).

Long-insert size: 1-25 kb with or without ELF-based size selection. Barcoded amplicons or barcoded genomic DNA: 0.5-10 kb. IsoSeq: from RNA or cDNA, with or without size-binning.

The SEQUEL has a sequencing output of 400k-600k polymerase reads (each 10-30 kb long) per SMRT cell. This instrument is ideal for de novo sequencing of any genome size. It also works well for sequencing IsoSeq libraries with or without size binning. Current (mid 2019) consumables and chemistry: LR v3 SMRT cell, v3 chemistry, SMRT Link 7.0

Service Price List

Last Updated: 08/2019

Illumina Services

Illumina Sequencing Runs

ServicesUFNon-ProfitCommercial
Illumina MiSeq: 1x150 cycles run (v3)1236.261390.791545.32
Illumina MiSeq:1x300 cycles run (v2)1399.841574.821749.80
Illumina MiSeq: 1x50 cycles run (v2)1118.271258.051397.83
Illumina MiSeq: 2x150 cycles run (v2)1399.841574.821749.80
Illumina MiSeq: 2x250 cycles run (v2)1512.291701.331890.36
Illumina MiSeq: 2x300 cycles run (v3)1910.512149.322388.13
Illumina MiSeq: 2x75 cycles run (v3)1236.311390.851545.38
Illumina MiSeq: 2x150 cycles run Micro flow cell$650.00$731.30$812.50
Illumina MiSeq: 2x150 cycles run Nano flow cell$495.00$556.90$618.80
Illumina MiSeq: 2x250 cycles run Nano flow cell$555.00$624.40$693.80
Illumina NextSeq500: High Throughput 1x150 cycles run3387.403810.824234.25
Illumina NextSeq500: High Throughput 1x75 cycles run2018.092270.362522.62
Illumina NextSeq500: High Throughput 2x150 cycles run5099.035736.416373.79
Illumina NextSeq500: High Throughput 2x75 cycles run3387.403810.824234.25
Illumina NextSeq500: MID Throughput 1x150 cycles run1647.511853.452059.39
Illumina NextSeq500: MID Throughput 2x150 cycles run2314.782604.122893.47
Illumina NextSeq500: MID Throughput 2x75 cycles run1647.511853.452059.39
Illumina: HiSeq3000, 2x150 run (one lane)2260.282542.822825.36
Illumina: HiSeq3000, 1x150 run (one lane)1435.441614.871794.30
Illumina: HiSeq3000, 2x75 run (one lane)1703.251916.152129.06
Illumina: HiSeq3000, 1x50 run (one lane)1065.871199.101332.34
Illumina: HiSeq3000, 2x100 run (one lane)1874.642108.972343.30
Illumina MiSeq, Load only runs (User provides all library QC data and sequencing kit, ICBR sets-up run), short runs170.00191.25212.50
Illumina MiSeq, Load only runs (User provides all library QC data and sequencing kit, ICBR sets-up run), standard runs288.75324.84360.94
Illumina NextSeq500, Load only runs (User provides all library QC data and sequencing kit, ICBR sets-up run)393.75442.97492.19

Illumina Library Construction

ServicesUFNon-ProfitCommercial
Illumina: Fragment library construction: single-read, paired-end, or barcoded (1-24). Fee per library170.00191.25212.50
Illumina: Fragment library construction: single-read, paired-end, or barcoded (24-48). Fee per library165.00185.63206.25
Illumina: Fragment library construction: single-read, paired-end, or barcoded (48-96). Fee per library160.00180.00200.00
Illumina: Bisulfite-converted, fragment library construction: single-read, paired-end, or barcoded (1-24). Fee shown is per library270.00303.75337.50
Illumina: Bisulfite-converted, fragment library construction: single-read, paired-end, or barcoded (25-48). Fee shown is per library260.00292.50325.00
Illumina: Enzymatic-methyl, fragment library construction: single-read, paired-end, or barcoded (1-24). Fee shown is per library235.00264.38293.75
Illumina Reduced Representation Bisulfite-Converted (RRBS) library (one).270.00303.75337.50
Illumina: PCR-free, fragment library construction: single-read, paired-end, or barcoded. Fee per library170.00191.25212.50
Illumina: Ultra-low input (0.2-5.0 ng), fragment library construction: single-read, paired-end, or barcoded. Fee per library200.00225.00250.00

Illumina Library Construction in Gene Expression & Genotyping Core

ServicesUFNon-ProfitCommercial
16 s library Automation Per Sample (batch w/48 samples)$74.00$83.25$92.50
16 s library Automation Per Sample (batch w/96 samples)$69.00$77.63$86.25
Illumina RNA-Seq libraries (Poly A)$220.00$265.00$280.00
Illumina RNA-Seq library Prep for low input, ribo-depletion or degraded RNA$320.00$380.00$480.00
Illumina RNASeq library Prep (polyA with 140 samples and up)$190.00$240.00$280.00
Illumina RNASeq library Prep (polyA with 500 samples and up)$135.00$185.00$225.00
Illumina Small RNA libray w/Barcode$300.00$354.00$375.00
NEB Directional RNASeq Library Preparation$220.00$260.00$300.00
NEB Non-directional RNASeq Library preparation$165.00$200.00$250.00
NextGen Sequencing Library Quantification$45.00$50.00$67.50
NextGen Sequencing Library Quantification (30 or more submitted at one time)$35.00$38.50$52.50
RNASeq library automation per sample (24 samples)$220.00$270.00$344.00
RNASeq library automation per sample (48 samples)$200.00$250.00$324.00
RNASeq library automation per sample (96 samples)$175.00$225.00$299.00
rRNAs Depletion$160.00$175.00$200.00
RNA Extraction$50.00$60.00$75.00
RNA Clean-up$22.00$33.00$44.00
Agilent SureSelect Target Enrichment$180.00$220.00$300.00
IDT Exome Capture Library Construction with Pre-pooling of 3 or less (per sample)$330.00$370.00$420.00
IDT Exome Capture Library Construction with Pre-pooling of 4 and up (per sample)$300.00$340.00$390.00
IDT Exome Capture Library Construction Without Pre-pooling (per sample)$370.00$410.00$460.00

PacBio Services

PacBio Sequencing Runs

ServicesUFNon-ProfitCommercial
PacBio SEQUEL 1-8 cells. Standard v3 SMRT cell. 10-hour movie. Fee per SMRT cell1595.001794.381993.75
PacBio SEQUEL 1-8 cells. LR v3 SMRT cell. 20-hour movie. Fee per SMRT cell1720.001935.002150.00
PacBio SEQUEL 9-32 cells. Standard v3 SMRT cell. 10-hour movie. Fee per SMRT cell1575.001771.881968.75
PacBio SEQUEL 9-32 cells. LR v3 SMRT cell. 20-hour movie. Fee per SMRT cell1700.001912.502125.00
PacBio SEQUEL >32 cells. Standard v3 SMRT cell. 10-hour movie. Fee per SMRT cell1550.001743.751937.50
PacBio SEQUEL >32 cells. LR v3 SMRT cell. 20-hour movie. Fee per SMRT cell1675.001884.382093.75

PacBio Library Construction

ServicesUFNon-ProfitCommercial
PacBio: Fragment library construction without size selection (1-24). Fee per library.400.00450.00500.00
PacBio: Fragment library construction without size selection (25-48). Fee per library.390.00438.75487.50
PacBio: Fragment library construction without size selection (49-96). Fee per library.380.00427.50475.00
PacBio: Fragment library construction with ELF size selection (1-24). Fee per library.632.65711.73790.81
PacBio: Fragment library construction with ELF size selection (25-48). Fee per library.615.60692.55769.50
PacBio: Fragment library construction with ELF size selection (49-96). Fee per library.600.80675.90751.00
PacBio: Barcoded genomic DNA adapter ligation intended for multiplexed sequencing, (1-24). Fee per sample.131.25147.66164.06
PacBio: Barcoded genomic DNA adapter ligation intended for multiplexed sequencing, (25-48). Fee per sample.127.05142.93158.81
PacBio: Barcoded genomic DNA adapter ligation intended for multiplexed sequencing, (49-96). Fee per sample.122.85138.21153.56
PacBio: Barcoded genomic DNA pool, damage repair, exonuclease and quantitation, (1-24). Fee per pool.336.00378.00420.00
PacBio: Barcoded amplicon adapter ligation intended for multiplexed sequencing, (1-24). Fee per sample.71.0079.8888.75
PacBio: Barcoded amplicon adapter ligation intended for multiplexed sequencing, (25-48). Fee per sample.69.0077.6386.25
PacBio: Barcoded amplicon adapter ligation intended for multiplexed sequencing, (49-96). Fee per sample.67.0075.3883.75
PacBio: Barcoded amplicon pool, damage repair, exonuclease and quantitation, (1-24). Fee per pool.294.00330.75367.50
PacBio: IsoSeq library construction from RNA (per sample), No size selection, one SMRT bell library.550.00618.75687.50
PacBio: IsoSeq library construction from RNA (per sample), large transcripts size selection and re-pooling, one SMRT bell library.925.001040.631156.25
PacBio: IsoSeq library construction from cDNA (per sample). No size selection, one SMRT bell library.475.00534.38593.75
PacBio: IsoSeq library construction from cDNA (per sample), large transcripts size selection and re-pooling, one SMRT bell library.880.00990.001100.00
PacBio, full-length 16S Metagenomics library construction, 16 samples. Starting from genomic DNA.2624.202952.233280.25
PacBio, full-length 16S Metagenomics library construction, 8 samples. Starting from genomic DNA.1604.001804.502005.00
PacBio, full-length 16S Metagenomics library construction, 16 samples. Starting from genomic DNA.2624.22952.233280.25
PacBio, full-length 16S Metagenomics library construction, 8 samples. Starting from genomic DNA.16041804.52005

10x Genomics Services

10X Genomics Library Construction

ServicesUFNon-ProfitCommercial
Illumina 10X Genomics, Linked-read library construction (one)$735$826.88$918.75
Illumina 10X Genomics, ATAC-Seq library construction (one)$1822$2049.75$2277.50

10X Genomics Library Construction in Genotyping & Gene Expression Core

ServicesUFNon-ProfitCommercial
10XGenomics Single Cell 3'/5' RNAseq Library Prep (per sample)$1,765.40 $1,985.06 $2,205.63
10XGenomics Single Human/Mouse T cell V(D)J Library Prep (per sample )$1,850.00 $2,081.25 $2,312.50

General Services

General Services

ServicesUFNon-ProfitCommercial
Amplicon or fragment library evaluation, 1-5 libraries (mass, size distribution and purity)89.25100.41111.56
Amplicon or fragment library evaluation, 6-10 libraries (mass, size distribution and purity)110.25124.03137.81
Amplicon or fragment library evaluation, 11-15 libraries (mass, size distribution and purity)141.75159.47177.19
Consultation0.000.000.00
Sequencing library construction workshop: Per participant fee. Offered when announced only.36.7541.3445.94
Covaris assisted use (One hour). No consumables provided. Call NDS lab to reserve instrument and time.21.0023.6326.25
Covaris microtubes12.6014.1815.75
Delivery media, data processing and handling (or unreturned drives)231.00259.88288.75
Fluorescence-based ds-DNA or ss-DNA by QUBIT: 1-11 samples24.1527.1730.19
Library pooling (2-12 libraries), plus QUBIT and TapeStation on the pool (User provides mass and size data on individual samples)126.00141.75157.50
Library pooling (13-24 libraries), plus QUBIT and TapeStation on the pool (User provides mass and size data on individual samples)183.75206.72229.69
Library pooling (25-48 libraries), plus QUBIT and TapeStation on the pool (User provides mass and size data on individual samples)283.50318.94354.38
Library pooling (49-96 libraries), plus QUBIT and TapeStation on the pool (User provides mass and size data on individual samples)472.50531.56590.63
MoBio PowerClean clean-up, per sample26.2529.5332.81
Processing and handling for re-delivery of data105.00118.13131.25
Library fragment selection (AMPure-based, QUBIT and bioanalyzer or TapeStation), 7-12 samples:228.22256.74285.27
Library fragment selection (AMPure-based, QUBIT and bioanalyzer or TapeStation), 2-6 samples:164.85185.46206.06
Library fragment selection (AMPure-based, QUBIT and bioanalyzer or TapeStation), One sample:112.35126.39140.44
Library or sample fragment selection (ELF-based). Fractions only. NO QUBIT, NO AMPure, NO Bioanalyzer (per sample)136.50153.56170.63
Library or sample fragment selection (ELF-based), with QUBIT, with Bioanalyzer, with AMPure on selected fraction only (per sample)257.25289.41321.56
Library or sample fragment selection: Gel-based, with QUBIT and Bioanalyzer257.25289.41321.56
Library or sample clean-up: AMPure only, per sample16.8018.9021.00
Fluorescence-based ds-DNA or ss-DNA by QUBIT: 1-11 samples24.1527.1730.19