CORE OVERVIEW

The Cytometry core offers project consulting, experimental design and optimization as well as data analysis services to help researchers with large-scale and small-scale experiments acquired on our sorters and cytometry instruments. We coordinate closely with other ICBR cores (Next-Gen Sequencing, Gene Expression, Bioinformatics, Monoclonal Antibody, Electron Microscopy and Proteomics) on shared projects. Our core offers a full suite of services from staff assisted services to training courses for all users, as well as instrument training for 24/7 access to our self-service equipment usage.

The Flow Cytometry core provides a variety of tools and staff expertise for both live and fixed cell analysis. The laboratory incorporates numerous flow cytometers, from simple entry-level instruments to high-end 5-laser, 17-parameter instruments as well as a spectral cytometer to analyze your cells. We have  3 FACS ARIA’s and a SONY SH800 sorters to help users sort  your cells of interest. In addition, the laboratory offers a suite of microscopy equipment for self-service users. The microscopes are well equipped with a variety of excitation lines from near UV to red and can collect a variety of emission colors (depending on dye combinations), with viable cell time lapse and extensive computed parameter capabilities, including FRET, FRAP, image stitching, and 3D reconstruction. We offer a host of Microscope brands from a Leica SP5 Confocal, a Nikon Multiphoton Confocal system, a Nikon Live cell Imaging platform, 2 Olympus spinning Disk Confocal instruments, as well as our new Keyence BZX800.

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