Cytometry

ICBR-Cytometry@ad.ufl.edu   ///  (352) 273-6032   ///    RRID:SCR_019119 
Cytometers, Confocal Microscopes, Histology, In-Vivo Imaging & More

CORE OVERVIEW

The Cytometry core offers project consultingexperimental design and optimization as well as data analysis services to help researchers with large-scale and small-scale experiments acquired on our sorters and cytometry instruments. We coordinate closely with other ICBR cores (Next-Gen Sequencing, Gene Expression, Bioinformatics, Monoclonal Antibody, Electron Microscopy and Proteomics) on shared projects. Our core offers a full suite of services from staff assisted services to training courses for all users, as well as instrument training for 24/7 access to our self-service equipment usage.

The Flow Cytometry core provides a variety of tools and staff expertise for both live and fixed cell analysis. The laboratory incorporates numerous flow cytometers, from simple entry-level instruments to high-end 5-laser, 17-parameter instruments as well as a spectral cytometer to analyze your cells. We have  3 FACS ARIA’s and a SONY SH800 sorters to help users sort  your cells of interest. In addition, the laboratory offers a suite of microscopy equipment for self-service users. The microscopes are well equipped with a variety of excitation lines from near UV to red and can collect a variety of emission colors (depending on dye combinations), with viable cell time lapse and extensive computed parameter capabilities, including FRET, FRAP, image stitching, and 3D reconstruction. We offer a host of Microscope brands from a Leica SP5 Confocal, a Nikon Multiphoton Confocal system, a Nikon Live cell Imaging platform, 2 Olympus spinning Disk Confocal instruments, as well as our new Keyence BZX800.

CORE NEWS

Dr. David Moraga: 30 Years of Advancing Scientific Excellence at ICBR Dr. David Moraga: 30 Years of Advancing Scientific Excellence at ICBR
Dr. David Moraga: 30 Years of Advancing Scientific Excellence at ICBR
Dr. David Moraga: 30 Years of Advancing Scientific Excellence at ICBR David Moraga, PhD, the NextGen Sequencing director at the ICBR, is retiring after 30 years of service. Since 1993, Dr. Moraga has played a vital role in advancing the center’s mission of providing cutting-edge scientific technology and research support services to the global scientific community. “Dr. David Moraga’s name has been almost synonymous with DNA sequencing for decades at ICBR and in the UF biotech communi...
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Dr. David Moraga: 30 Years of Advancing Scientific Excellence at ICBR
Labor Day Notice: ICBR Closed on September 4th Labor Day Notice: ICBR Closed on September 4th
Labor Day Notice: ICBR Closed on September 4th
Labor Day Notice: ICBR Closed on September 4th Please note that the ICBR will be closed on September 4th in observance of Labor Day. Kindly be aware that we will not be able to accept any packages or samples during this time. We will resume regular operations on the following business day. Please refer to our staff directory for additional details. ...
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Labor Day Notice: ICBR Closed on September 4th
ICBR Closed Wednesday, August 30th for Tropical Storm Idalia ICBR Closed Wednesday, August 30th for Tropical Storm Idalia
ICBR Closed Wednesday, August 30th for Tropical Storm Idalia
ICBR Closed Wednesday, August 30th for Tropical Storm Idalia Due to Tropical Storm Idalia, the ICBR will close beginning at noon on (8/29) through Wednesday (8/30). We will not be accepting packages or samples during this time. The university expects to make an announcement on Wednesday regarding the resumption of normal campus operations. Please visit www.ufl.edu for updates and information on storm preparation.   Please refer to our staff directory for additional details. ...
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ICBR Closed Wednesday, August 30th for Tropical Storm Idalia
ICBR Cytometry Acquires BD FACSAria Fusion ICBR Cytometry Acquires BD FACSAria Fusion
ICBR Cytometry Acquires BD FACSAria Fusion
BD FACSAria Fusion Has Arrived at ICBR Cytometry The BD FACSAria Fusion cell sorter has UV, Violet, Blue, Yellow/Green and Red lasers and can detect up to 18 colors. This instrument can simultaneously sort 4 different populations into 1.5, 5 or 15 mL tubes or 96-well plates. BD FACSAria Fusion has precisely integrated fluidic and optical systems to maximize signal detection. It is also integrated into a biosafety cabinet and includes an aerosol management system to enable sterile sorting. As Fus...
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ICBR Cytometry Acquires BD FACSAria Fusion
Dr. Alberto Riva at ISMB/ECCB2023: Advancing Bioinformatics Cores and Inspiring Collaboration Dr. Alberto Riva at ISMB/ECCB2023: Advancing Bioinformatics Cores and Inspiring Collaboration
Dr. Alberto Riva at ISMB/ECCB2023: Advancing Bioinformatics Cores and Inspiring Collaboration
Audience at the BioInfo Core Session at ISMB 2023 Dr. Alberto Riva at ISMB/ECCB2023: Advancing Bioinformatics Cores and Inspiring Collaboration Dr. Alberto Riva, the Scientific Director for the ICBR Bioinformatics core, recently attended the highly acclaimed Intelligent Systems for Molecular Biology (ISMB) conference, renowned as the largest and most prestigious gathering in the field of computational biology and bioinformatics.  This year’s conference, held in Lyon, France in partnership w...
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Dr. Alberto Riva at ISMB/ECCB2023: Advancing Bioinformatics Cores and Inspiring Collaboration
ICBR’s Dr. Anna-Lisa Paul Honored as Lifetime Fellow by the AAAS ICBR’s Dr. Anna-Lisa Paul Honored as Lifetime Fellow by the AAAS
ICBR's Dr. Anna-Lisa Paul Honored as Lifetime Fellow by the AAAS
The American Association for the Advancement of Science honors 19 UF faculty as Lifetime Fellows Dr. Anna-Lisa Paul, alongside 18 other UF faculty, has been honored as a Lifetime Fellow of AAAS. Dr. Paul was honored for “distinguished contributions to the understanding of life’s ability to move beyond planet Earth, as well as extraordinary service to the space biology science community”. Her research focuses on how plants respond to environmental change, with emphasis on spaceflight...
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ICBR’s Dr. Anna-Lisa Paul Honored as Lifetime Fellow by the AAAS
Surviving Space: Extreme Plant Adaptation Surviving Space: Extreme Plant Adaptation
Surviving Space: Extreme Plant Adaptation
Which plants have the best chances for survival in space? That’s the question being addressed by Principal Investigators Anna-Lisa Paul and Robert Ferl in the Epigenetic Adaptation to the Spaceflight Environment – Accumulated Genomic Change Induced by Generations in Space (Plant Habitat-03) investigation which will soon launch to the International Space Station aboard Northrop Grumman’s 18th commercial resupply services mission for NASA. “One of the exciting things about space biology ...
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Surviving Space: Extreme Plant Adaptation
ICBR Cytometry Acquires BD FACS Symphony S6 ICBR Cytometry Acquires BD FACS Symphony S6
ICBR Cytometry Acquires BD FACS Symphony S6
ICBR Cytometry Acquires BD FACS Symphony S6 The recent acquisition of the BD FACS Symphony S6 cell sorter in the ICBR Cytometry Core will expand the capabilities of the UF research community to isolate rare and unique populations of cells from a complex, mixed populations.  Cell sorting on the S6 instrument facilitates the isolation of enriched cell pools and individual cells for downstream genomic analysis using DNA sequencing, as well as RNA-seq analysis to identify, quantify and profile mRNA...
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ICBR Cytometry Acquires BD FACS Symphony S6

CORE INSTRUMENTS

Cytometry