Cytometry

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Overview

Important COVID-19 news for ICBR Cytometry and CTAC Users:

    • ICBR Cytometry remains open to serve research deemed essential by UF Research.
    • User trainings on all instruments in CY and CTAC are now available.
    • We ask all users to have only the one person with the self-service reservation come into the core to operate the equipment- this helps with social distancing
    • We ask that all self service users wash their hands upon arrival to the core lab and put on gloves before touching computers or instruments.
    • We ask that all self service users clean the computer keyboard, mice and the instrument with sanitizing wipes and 70% ethanol when you are done running samples.
    • We ask that all sorting samples for drop off have ethanol resistant labels on the tubes and a written sample ID key for the Sorter operator.
    • We ask that our users please practice “social distancing” when setting up sorting’s with the CY staff.

The Cytometry core offers project consultingexperimental design and optimization as well as data analysis services to help researchers with large-scale and small-scale experiments acquired on our sorters and cytometry instruments. We coordinate closely with other ICBR cores (Next-Gen Sequencing, Gene Expression, Bioinformatics, Monoclonal Antibody, Electron Microscopy and Proteomics) on shared projects. Our core offers a full suite of services from staff assisted services to training courses for all users, as well as instrument training for 24/7 access to our self-service equipment usage.

The Flow Cytometry core provides a variety of tools and staff expertise for both live and fixed cell analysis. The laboratory incorporates numerous flow cytometers, from simple entry-level instruments to high-end 5-laser, 17-parameter instruments as well as a spectral cytometer to analyze your cells. We have  3 FACS ARIA’s and a SONY SH800 sorters to help users sort  your cells of interest. In addition, the laboratory offers a suite of microscopy equipment for self-service users. The microscopes are well equipped with a variety of excitation lines from near UV to red and can collect a variety of emission colors (depending on dye combinations), with viable cell time lapse and extensive computed parameter capabilities, including FRET, FRAP, image stitching, and 3D reconstruction. We offer a host of Microscope brands from a Leica SP5 Confocal, a Nikon Multiphoton Confocal system, a Nikon Live cell Imaging platform, 2 Olympus spinning Disk Confocal instruments, as well as our new Keyence BZX800.

 

Core News

UF | ICBR Scientists Contribute to Clinical Trial Testing Novel Treatment for Canine Atopic Dermatitis UF | ICBR Scientists Contribute to Clinical Trial Testing Novel Treatment for Canine Atopic Dermatitis
UF | ICBR Scientists Contribute to Clinical Trial Testing Novel Treatment for Canine Atopic Dermatitis
  Atopic dermatitis, or AD, is a common chronic inflammatory skin disease affecting dogs, causing defects of the skin barrier and modifications of the normal cutaneous microflora. While multiple treatment options are available, there is a growing interest in the use of alternative natural ingredient products. In human AD reports show the potential benefits of topical applications of heat-killed beneficial bacteria, with some studies demonstrating the efficacy of rapidly killed beneficial la...
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UF | ICBR Scientists Contribute to Clinical Trial Testing Novel Treatment for Canine Atopic Dermatitis
UF researchers to use AI to predict how hurricanes spread invasive plants UF researchers to use AI to predict how hurricanes spread invasive plants
UF researchers to use AI to predict how hurricanes spread invasive plants
Courtesy of UF/IFAS Written by Brad Buck Scientists project hurricane intensity and frequency will increase with climate change. That leads researchers to want to better predict how storms will disperse and establish nonnative plant invaders. Knowing where invasive plants spread will always be useful. But it may become especially handy soon as the National Hurricane Center is considering moving the official start of the Atlantic hurricane season two weeks earlier, starting it May 15, instead of ...
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UF researchers to use AI to predict how hurricanes spread invasive plants
World-Renowned UF Health Biochemistry Researcher Passes Away World-Renowned UF Health Biochemistry Researcher Passes Away
World-Renowned UF Health Biochemistry Researcher Passes Away
Courtesy of College of Medicine News March 3, 2021 — University of Florida Health biochemistry professor Mavis Agbandje-McKenna, Ph.D., whose world-renowned work on the detailed structure of viruses led to advances in gene therapy treatments for different diseases, died Wednesday at her home near Gainesville of the neurodegenerative disease amyotrophic lateral sclerosis. She was 57. Agbandje-McKenna’s work with the small, infectious particles had a worldwide impact, allowing other scientists to ...
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World-Renowned UF Health Biochemistry Researcher Passes Away
UF Health pilot program to deliver COVID-19 monoclonal antibody therapy to rural communities UF Health pilot program to deliver COVID-19 monoclonal antibody therapy to rural communities
UF Health pilot program to deliver COVID-19 monoclonal antibody therapy to rural communities
Courtesy of UF Health Written by Diana Tonnessen A University of Florida Health pilot program will bring a new COVID-19 monoclonal antibody therapy to rural communities in North Florida during the coming weeks. As part of the White House’s Operation Warp Speed initiative, the U.S. Department of Health and Human Services selected UF Health and the UF Clinical and Translational Science Institute to coordinate the distribution and administration of 1,000 doses of bamlanivimab, a monoclonal antibody...
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UF Health pilot program to deliver COVID-19 monoclonal antibody therapy to rural communities
UF offers powerful new NVIDIA supercomputer to students and researchers across State University System UF offers powerful new NVIDIA supercomputer to students and researchers across State University System
UF offers powerful new NVIDIA supercomputer to students and researchers across State University System
Courtesy of UF NEWS Written by Brittany Wise University of Florida’s new supercomputer — the fastest artificial intelligence supercomputer in higher education — will soon be available for students and faculty across the State University System, UF Provost Joe Glover announced today. The computer will give faculty and students from across the State unparalleled computing power in artificial intelligence (AI) to apply across many areas to improve lives, bolster industry, and create ec...
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UF offers powerful new NVIDIA supercomputer to students and researchers across State University System
New Approach Isolating Stem Cells Could Lead to Breakthrough In Regenerative Medicine New Approach Isolating Stem Cells Could Lead to Breakthrough In Regenerative Medicine
New Approach Isolating Stem Cells Could Lead to Breakthrough In Regenerative Medicine
A recent study conducted at the University of Florida could transform the way we isolate stem cells, validating a method that takes only minutes and can be performed just about anywhere. Stem cells are “immature” cells that have the unique ability to develop into other cell types.  For example, hematopoietic stem cells (HSCs) regenerate blood cells and mesenchymal stem cells (MSCs) regenerate cells of solid tissues. Because HSCs and MSCs are often used for clinical regenerative medicine, isolati...
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New Approach Isolating Stem Cells Could Lead to Breakthrough In Regenerative Medicine
Novel CD33 Antibodies Unravel Localization, Biology, and Therapeutic Implications of CD33 Isoforms Novel CD33 Antibodies Unravel Localization, Biology, and Therapeutic Implications of CD33 Isoforms
Novel CD33 Antibodies Unravel Localization, Biology, and Therapeutic Implications of CD33 Isoforms
UF | ICBR  Drs. Angel Sampson and Andria Doty recently teamed up with Dr. Jatinder Lamba in the Department of Pharmacotherapy and Translational Research in the UF College of Pharmacy, and  Dr. Soheil Meshinchi at the Fred Hutchinson Cancer Research Center, University of Washington, in an effort to further understand the therapeutic relevance of various forms of the CD33 protein as it relates to the antibody-drug therapy.  CD33 is expressed on malignant cancer cells in nearly all patients with ac...
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Novel CD33 Antibodies Unravel Localization, Biology, and Therapeutic Implications of CD33 Isoforms
Featured Video: Black Voices in Research Featured Video: Black Voices in Research
Featured Video: Black Voices in Research
 As part of its mission to facilitate honest and vulnerable conversations about race, the University of Florida Clinical and Translational Science Institute Diversity and Cultural Competence Council (UF CTSI DC3) presents its inaugural Black Voices in Research Storytelling Event. This event and others like it will serve to underscore the inherent value of sharing personal stories and the role that these stories can play in creating and promoting an inclusive, diverse, equitable, and accessible ...
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Featured Video: Black Voices in Research

Instruments

Staff

NameTitleTelephoneE-mailRoom
Andria DotyCytometry Scientific Director(352) 273-8186sun30@ufl.edu292B CGRC
Mariza MirandaCytometry Core Research Specialist II(352) 273-6032marizamiranda@ufl.edu292 CGRC
Anthony BoullosaCytometry Core Research Technician III(352) 273-6032afbfl@ufl.edu292 CGRC
Doug SmithCTAC Manager(352) 392-1745dugsmith@ufl.eduLG-164
Alexander Linares OPS Cytometry Lab Technician(352) 273-6032a.linares@ufl.edu292 CGRC