Cytometry

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Overview

The Cytometry core offers project consultingexperimental design and optimization as well as data analysis services to help researchers with large-scale and small-scale experiments acquired on our sorters and cytometry instruments. We coordinate closely with other ICBR cores (Next-Gen Sequencing, Gene Expression, Bioinformatics, Monoclonal Antibody, Electron Microscopy and Proteomics) on shared projects. Our core offers a full suite of services from staff assisted services to training courses for all users, as well as instrument training for 24/7 access to our self-service equipment usage.

The Flow Cytometry core provides a variety of tools and staff expertise for both live and fixed cell analysis. The laboratory incorporates numerous flow cytometers, from simple entry-level instruments to high-end 5-laser, 17-parameter instruments as well as a spectral cytometer to analyze your cells. We have  3 FACS ARIA’s and a SONY SH800 sorters to help users sort  your cells of interest. In addition, the laboratory offers a suite of microscopy equipment for self-service users. The microscopes are well equipped with a variety of excitation lines from near UV to red and can collect a variety of emission colors (depending on dye combinations), with viable cell time lapse and extensive computed parameter capabilities, including FRET, FRAP, image stitching, and 3D reconstruction. We offer a host of Microscope brands from a Leica SP5 Confocal, a Nikon Multiphoton Confocal system, a Nikon Live cell Imaging platform, 2 Olympus spinning Disk Confocal instruments, as well as our new Keyence BZX800.

 

Core News

ICBR Bioinformatics Contributes to Landmark UFHCC Publication ICBR Bioinformatics Contributes to Landmark UFHCC Publication
ICBR Bioinformatics Contributes to Landmark UFHCC Publication
The ICBR Bioinformatics Core contributed to a high-profile paper from the cancer research laboratory of UFHCC Director Dr. Jonathan Licht, recently published in Cancer Discovery. The paper, entitled “A Mutation in Histone H2B Represents a New Class of Oncogenic Driver”, describes how mutations in core histones disrupt nucleosome stability, leading to disregulation of gene expression and acting as a novel oncogenic mechanism. As part of this study the investigators used a technique ca...
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ICBR Bioinformatics Contributes to Landmark UFHCC Publication
Help ICBR Grow in Ten Quick Questions! Help ICBR Grow in Ten Quick Questions!
Help ICBR Grow in Ten Quick Questions!
We want to hear about your experience working with UF ICBR. What do you like? How can we improve? We want to hear from you. For your convenience, we kept our survey to ten quick questions. This is your opportunity to help us continue our quest to become the best resource on campus. Click Here to Participate in our Survey ...
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Help ICBR Grow in Ten Quick Questions!
Instrument Spotlight: Protein Simple Wes Instrument Spotlight: Protein Simple Wes
Instrument Spotlight: Protein Simple Wes
Wes™ lets you separate and analyze proteins by size from 2-440 kDa. He also gets you down to pg-level sensitivity with just 3 µL of starting material. Got a lot of samples and no time? No problem. Wes runs up to 25 samples in 3 hours flat and gives you quantitative, size-based data including total protein. Don’t wait to discover, get going today with Wes! Our Simple Western systems offer the right throughput for your needs. Whether you are looking to process up to 24 samples per run or ...
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Instrument Spotlight: Protein Simple Wes
Instrument Spotlight: PacBio Sequel Instrument Spotlight: PacBio Sequel
Instrument Spotlight: PacBio Sequel
GAIN A COMPREHENSIVE VIEW OF GENOMES, TRANSCRIPTOMES, AND EPIGENOMES – Create high-quality whole genome de novo assemblies of eukaryotic organisms – Survey large population cohorts and resolve structural variants – Read full-length transcripts to characterize isoform diversity – Target all variant types across relevant genomic regions – Detect epigenetic modifications simultaneously Get Started: Contact ICBR NextGen DNA Sequencing Click here to see SEQUEL Services &...
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Instrument Spotlight: PacBio Sequel
ICBR Closed: September 2nd and September 3rd ICBR Closed: September 2nd and September 3rd
ICBR Closed: September 2nd and September 3rd
ICBR will be closed on Monday, September 2nd in observation of Labor Day, and on Tuesday, September 3rd due to Hurricane Dorian. There is a chance we will be closed on Wednesday; please confirm our availability via phone or email. ...
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ICBR Closed: September 2nd and September 3rd
Single Cell Genomics on the 10X Genomics Chromium System Single Cell Genomics on the 10X Genomics Chromium System
Single Cell Genomics on the 10X Genomics Chromium System
The ICBR and UFHCC have partnered in the acquisition of new cutting-edge technology with the capability of analyzing gene structure and gene activity at the single cell level. The Chromium instrument developed by California-based 10X Genomics inc. provides two unique key technologies to the UF research community. In one application, the instrument uses synthetic gel beads to partition thousands of separate enzymatic reactions necessary for downstream DNA sequencing. In another application, short...
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Single Cell Genomics on the 10X Genomics Chromium System
CRISPR at UF ICBR CRISPR at UF ICBR
CRISPR at UF ICBR
In collaboration with the UFHCC, ICBR is pleased to announce several new CRISPR genome editing services now available to the UF research community. The CRISPR/Cas9 system allows researchers to easily introduce deletions, insertions and edited DNA sequences into mammalian cell genomes. It can be used to introduce very specific surgical edits to any gene and can in fact introduce foreign genes into specific regions of the animal cell genome. At the University of Florida, CRISPR is used within re...
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CRISPR at UF ICBR
Is Your Monoclonal Antibody Authentic? Confirming Hybridoma Cell Line Identity by IgC cDNA Sequencing Is Your Monoclonal Antibody Authentic? Confirming Hybridoma Cell Line Identity by IgC cDNA Sequencing
Is Your Monoclonal Antibody Authentic? Confirming Hybridoma Cell Line Identity by IgC cDNA Sequencing
Widespread reports of irreproducibility in health sciences research has prompted the NIH to update new grant application guidelines to include a brief description of methods that will be used to ensure identity and validity of key biological and/or chemical resources used in the proposed study. In this regard there is a significant effort now underway using DNA “fingerprinting” as a way to use microsatellite repeat lengths as a powerful tool for human cell line authentication. This method ha...
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Is Your Monoclonal Antibody Authentic? Confirming Hybridoma Cell Line Identity by IgC cDNA Sequencing

Instruments

Staff

NameTitleTelephoneE-mailRoom
Andria DotyCytometry Scientific Director(352) 273-8186sun30@ufl.edu292B CGRC
Mariza MirandaCytometry Core Research Specialist II(352) 273-6032marizamiranda@ufl.edu292 CGRC
Anthony BoullosaCytometry Core Research Technician III(352) 273-6032afbfl@ufl.edu292 CGRC
Kimberly Backer-KelleyCore Research Technician II(352) 273-5341klbk@ufl.edu185 CGRC
Doug SmithCTAC Manager(352) 392-1745dugsmith@ufl.edu
Gary BrownCTAC Lab Technician(352) 273-6569gabrown@ufl.edu