Cytometry

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Overview

The Cytometry core offers project consultingexperimental design and optimization as well as data analysis services to help researchers with large-scale and small-scale experiments acquired on our sorters and cytometry instruments. We coordinate closely with other ICBR cores (Next-Gen Sequencing, Gene Expression, Bioinformatics, Monoclonal Antibody, Electron Microscopy and Proteomics) on shared projects. Our core offers a full suite of services from staff assisted services to training courses for all users, as well as instrument training for 24/7 access to our self-service equipment usage.

The Flow Cytometry core provides a variety of tools and staff expertise for both live and fixed cell analysis. The laboratory incorporates numerous flow cytometers, from simple entry-level instruments to high-end 5-laser, 17-parameter instruments as well as a spectral cytometer to analyze your cells. We have  3 FACS ARIA’s and a SONY SH800 sorters to help users sort  your cells of interest. In addition, the laboratory offers a suite of microscopy equipment for self-service users. The microscopes are well equipped with a variety of excitation lines from near UV to red and can collect a variety of emission colors (depending on dye combinations), with viable cell time lapse and extensive computed parameter capabilities, including FRET, FRAP, image stitching, and 3D reconstruction. We offer a host of Microscope brands from a Leica SP5 Confocal, a Nikon Multiphoton Confocal system, a Nikon Live cell Imaging platform, 2 Olympus spinning Disk Confocal instruments, as well as our new Keyence BZX800.

 

Core News

ICBR Faculty Director to Speak at 2019 Florida Genetics Symposium ICBR Faculty Director to Speak at 2019 Florida Genetics Symposium
ICBR Faculty Director to Speak at 2019 Florida Genetics Symposium
We are excited to announce that Sixue Chen, PhD will present at the 2019 Florida Genetics Symposium, presented by the University of Florida Genetics Institute. This two day event on November 4th and 5th will bring together some of the brightest minds in genetics. Faculty Director of the ICBR Proteomics & Mass Spectrometry Core, Dr. Chen will present “Single Cell-Type Proteomics and Metabolomics: New Insights into Guard Cell Umminuty and CO2 Responses” on Monday, November 4th from 1:45pm ...
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ICBR Faculty Director to Speak at 2019 Florida Genetics Symposium
The Beast is Here!  ICBR Launches the NovaSeq 6000 DNA Sequencer The Beast is Here! ICBR Launches the NovaSeq 6000 DNA Sequencer
The Beast is Here! ICBR Launches the NovaSeq 6000 DNA Sequencer
Dr. Steven Madore, ICBR Associate Director for Science and UFHCC Associate Director for Shared Resources, next to the new NovaSeq6000 (“the Beast“) with ICBR NGS Core Scientific Director Dr. David Moraga. The ICBR NextGen DNA Sequencing Core provides cutting edge instrumentation and technical expertise required to support campus-wide research activites. Established in 1987, the NGS Core has contributed to significant scientific discoveries on campus. For example, did you know that the first...
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The Beast is Here!  ICBR Launches the NovaSeq 6000 DNA Sequencer
The Luminex FLEXMAP 3D®  System – A Multiplexed Analytical Platform for  Novel Biomarker Discovery The Luminex FLEXMAP 3D® System – A Multiplexed Analytical Platform for Novel Biomarker Discovery
The Luminex FLEXMAP 3D® System – A Multiplexed Analytical Platform for Novel Biomarker Discovery
Figure 1. Luminex bead architecture technology ICBR Proteomics & Mass Spectrometry (PM) Core is now offering access to the Luminex FLEXMAP 3D®   analytical platform for discovering and validation of protein biomarkers across a wide range of diseases. The FLEXMAP 3D® system combines differentially dyed fluorescent microsphere sets with an innovative instrument design to enable simultaneous measurement of  the concentration of a large number of different analytes in a rapid multiplexed as...
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The Luminex FLEXMAP 3D®  System – A Multiplexed Analytical Platform for  Novel Biomarker Discovery
Dr. Anna-Lisa Paul receives NASA’s Exceptional Scientific Achievement Medal Dr. Anna-Lisa Paul receives NASA’s Exceptional Scientific Achievement Medal
Dr. Anna-Lisa Paul receives NASA's Exceptional Scientific Achievement Medal
Congratulations to UF’s Dr. Anna-Lisa Paul on receiving the NASA Exceptional Scientific Achievement Medal this past September! The prestigious award is reserved for individual efforts that are in line with NASA’s mission and have resulted in considerable scientific discovery or resulted in contributions of fundamental importance in their field or significantly improved the understanding of their field. Dr. Paul is the Interim Director at UF ICBR and Research Professor in the UF Horticultural...
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Dr. Anna-Lisa Paul receives NASA’s Exceptional Scientific Achievement Medal
Introducing Core Directors’ Open Consultation Meetings Introducing Core Directors’ Open Consultation Meetings
Introducing Core Directors' Open Consultation Meetings
In a continued effort to provide accessible and specialized expertise to our users, UF ICBR is proud to announce our Core Directors’ Open Consultation Meetings. Each month we will be available for a two hour walk-in session to discuss the best approaches and strategies for using ICBR technologies and expertise to drive your research forward. Our first meeting will be on Monday, October 7th from 11:00am to 1:00pm in CGRC 189. No appointment is require nor do you have to be a current ICBR user t...
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Introducing Core Directors’ Open Consultation Meetings
The NovaSeq 6000 has arrived at UF ICBR! The NovaSeq 6000 has arrived at UF ICBR!
The NovaSeq 6000 has arrived at UF ICBR!
After much anticipation, the Illumina NovaSeq 6000 has arrived at UF ICBR. Users can expect access later this fall. The NovaSeq 6000 Sequencing System unleashes groundbreaking innovations that leverage our proven technology. Now you can get scalable throughput and flexibility for virtually any sequencing method, genome, and scale of project. The NovaSeq will be housed in our NextGen DNA Sequencing core. Stay tuned for more information and events this fall. For more information on the instrument...
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The NovaSeq 6000 has arrived at UF ICBR!
ICBR Bioinformatics Contributes to Landmark UFHCC Publication ICBR Bioinformatics Contributes to Landmark UFHCC Publication
ICBR Bioinformatics Contributes to Landmark UFHCC Publication
The ICBR Bioinformatics Core contributed to a high-profile paper from the cancer research laboratory of UFHCC Director Dr. Jonathan Licht, recently published in Cancer Discovery. The paper, entitled “A Mutation in Histone H2B Represents a New Class of Oncogenic Driver”, describes how mutations in core histones disrupt nucleosome stability, leading to disregulation of gene expression and acting as a novel oncogenic mechanism. As part of this study the investigators used a technique ca...
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ICBR Bioinformatics Contributes to Landmark UFHCC Publication
Help ICBR Grow in Ten Quick Questions! Help ICBR Grow in Ten Quick Questions!
Help ICBR Grow in Ten Quick Questions!
We want to hear about your experience working with UF ICBR. What do you like? How can we improve? We want to hear from you. For your convenience, we kept our survey to ten quick questions. This is your opportunity to help us continue our quest to become the best resource on campus. Click Here to Participate in our Survey ...
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Help ICBR Grow in Ten Quick Questions!

Instruments

Staff

NameTitleTelephoneE-mailRoom
Andria DotyCytometry Scientific Director(352) 273-8186sun30@ufl.edu292B CGRC
Mariza MirandaCytometry Core Research Specialist II(352) 273-6032marizamiranda@ufl.edu292 CGRC
Anthony BoullosaCytometry Core Research Technician III(352) 273-6032afbfl@ufl.edu292 CGRC
Kimberly Backer-KelleyCore Research Technician II(352) 273-5341klbk@ufl.edu185 CGRC
Doug SmithCTAC Manager(352) 392-1745dugsmith@ufl.edu
Gary BrownCTAC Lab Technician(352) 273-6569gabrown@ufl.edu