Cytometry

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Overview

Important COVID-19 news for ICBR Cytometry and CTAC Users:

    • ICBR Cytometry remains open to serve research deemed essential by UF Research.
    • User trainings on all instruments in CY and CTAC are now available.
    • We ask all users to have only the one person with the self-service reservation come into the core to operate the equipment- this helps with social distancing
    • We ask that all self service users wash their hands upon arrival to the core lab and put on gloves before touching computers or instruments.
    • We ask that all self service users clean the computer keyboard, mice and the instrument with sanitizing wipes and 70% ethanol when you are done running samples.
    • We ask that all sorting samples for drop off have ethanol resistant labels on the tubes and a written sample ID key for the Sorter operator.
    • We ask that our users please practice “social distancing” when setting up sorting’s with the CY staff.

The Cytometry core offers project consultingexperimental design and optimization as well as data analysis services to help researchers with large-scale and small-scale experiments acquired on our sorters and cytometry instruments. We coordinate closely with other ICBR cores (Next-Gen Sequencing, Gene Expression, Bioinformatics, Monoclonal Antibody, Electron Microscopy and Proteomics) on shared projects. Our core offers a full suite of services from staff assisted services to training courses for all users, as well as instrument training for 24/7 access to our self-service equipment usage.

The Flow Cytometry core provides a variety of tools and staff expertise for both live and fixed cell analysis. The laboratory incorporates numerous flow cytometers, from simple entry-level instruments to high-end 5-laser, 17-parameter instruments as well as a spectral cytometer to analyze your cells. We have  3 FACS ARIA’s and a SONY SH800 sorters to help users sort  your cells of interest. In addition, the laboratory offers a suite of microscopy equipment for self-service users. The microscopes are well equipped with a variety of excitation lines from near UV to red and can collect a variety of emission colors (depending on dye combinations), with viable cell time lapse and extensive computed parameter capabilities, including FRET, FRAP, image stitching, and 3D reconstruction. We offer a host of Microscope brands from a Leica SP5 Confocal, a Nikon Multiphoton Confocal system, a Nikon Live cell Imaging platform, 2 Olympus spinning Disk Confocal instruments, as well as our new Keyence BZX800.

 

Core News

Novel CD33 Antibodies Unravel Localization, Biology, and Therapeutic Implications of CD33 Isoforms Novel CD33 Antibodies Unravel Localization, Biology, and Therapeutic Implications of CD33 Isoforms
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UF | ICBR  Drs. Angel Sampson and Andria Doty recently teamed up with Dr. Jatinder Lamba in the Department of Pharmacotherapy and Translational Research in the UF College of Pharmacy, and  Dr. Soheil Meshinchi at the Fred Hutchinson Cancer Research Center, University of Washington, in an effort to further understand the therapeutic relevance of various forms of the CD33 protein as it relates to the antibody-drug therapy.  CD33 is expressed on malignant cancer cells in nearly all patients with ac...
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Novel CD33 Antibodies Unravel Localization, Biology, and Therapeutic Implications of CD33 Isoforms
Featured Video: Black Voices in Research Featured Video: Black Voices in Research
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 As part of its mission to facilitate honest and vulnerable conversations about race, the University of Florida Clinical and Translational Science Institute Diversity and Cultural Competence Council (UF CTSI DC3) presents its inaugural Black Voices in Research Storytelling Event. This event and others like it will serve to underscore the inherent value of sharing personal stories and the role that these stories can play in creating and promoting an inclusive, diverse, equitable, and accessible ...
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UF | ICBR Staff Answer Challenge to Support Local Students in Need UF | ICBR Staff Answer Challenge to Support Local Students in Need
UF | ICBR Staff Answer Challenge to Support Local Students in Need
For the third consecutive year, UF | ICBR held their annual food drive to provide food and supplies to Gainesville grade schools (event concluded 12/4). Well, although our results were tremendous in 2018 and 2019, this year took the cake. Over the course of eight weeks, our staff of 46 individuals collected over 5,150 items for Gainesville students, or approximately 112 items per employee. UF | ICBR’s HR Manager, Erica Boyd, led the charge; generating buzz, building rapport with nearby sch...
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Important Year-End Dates for UF | ICBR Users Important Year-End Dates for UF | ICBR Users
Important Year-End Dates for UF | ICBR Users
As 2020 comes to a close, ICBR’s upcoming billing schedule will be altered as follows: 12/14/2020 – Last invoices generated in 2020 12/21/2020 at noon – Deadline to submit disputes for 2020 payment. If you have expiring funds, please work with your core laboratory contact to make sure your project is completed and billed before the 12/14/2020 invoices are posted. Core-Specific Dates and Deadlines Cytometry 12/22/2020 – CY labs, both CGRC and MBI, will shut down for the holiday break and...
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Important Year-End Dates for UF | ICBR Users
Excessive Heat Stroke Induces Long‐term Physiological Changes That are Linked to Altered Epigenetic Profiles Excessive Heat Stroke Induces Long‐term Physiological Changes That are Linked to Altered Epigenetic Profiles
Excessive Heat Stroke Induces Long‐term Physiological Changes That are Linked to Altered Epigenetic Profiles
Epigenetics is the study of heritable phenotypic change that involves chemical modifications to the DNA genome sequence or associated DNA-binding proteins. These specific alterations affect how cells turn genes off or on. Exertional heat stroke (EHS) can cause long-term negative effects on health. Although epigenetic “reprogramming” of the genome is involved in the response to heat acclimation, it is not known whether the long-term effects of EHS are mediated by epigenetic reprogramming. In a st...
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Excessive Heat Stroke Induces Long‐term Physiological Changes That are Linked to Altered Epigenetic Profiles
Lots of Samples, Little Time? Try Protein Simple Wes Lots of Samples, Little Time? Try Protein Simple Wes
Lots of Samples, Little Time? Try Protein Simple Wes
Unlike traditional western blots, the Protein Simple Wes at ICBR Monoclonal Antibody runs up to 25 samples in 3 hours flat and gives you quantitative, size-based data including total protein. With a long list of applications, including cancer, SARS-CoV-2, cell & gene therapy, targeted degradation and more, there is no shortage of opportunities. The same Protein Simple Wes appeared in a recent publication featured in Nature Communications from Dr. Shinichi Someya’s research group in the Depar...
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Are BPA Alternatives Safer? New Research Takes a Look Are BPA Alternatives Safer? New Research Takes a Look
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New research conducted at the University of Florida shows that a bisphenol A (BPA) alternative for making plastics, bisphenol S (BPS), could come with its own set of health risks. After past studies revealed the widely used BPA was contaminating food and water supplies, consumers quickly searched for alternatives. Market demand for BPA-free products led to the emergence of BPS as a commonly used bisphenol analog in plastic products. The UF | ICBR Proteomics and Mass Spectrometry (PM) core contri...
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UPDATE: UF | ICBR Continues to Provide Support to UF Community UPDATE: UF | ICBR Continues to Provide Support to UF Community
UPDATE: UF | ICBR Continues to Provide Support to UF Community
With the progression of recent events, there are a lot of questions about how it affects ICBR users. Please see below, important changes that could impact your experience at ICBR. These changes will be in place for the foreseeable future. Please refer back to this page for timely updates. As always, check the UF dedicated site for campus-wide information and initiatives. LATEST NEWS ICBR is now open, Monday through Friday, to support research on the University of Florida campus. Please be...
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UPDATE: UF | ICBR Continues to Provide Support to UF Community

Instruments

Staff

NameTitleTelephoneE-mailRoom
Andria DotyCytometry Scientific Director(352) 273-8186sun30@ufl.edu292B CGRC
Mariza MirandaCytometry Core Research Specialist II(352) 273-6032marizamiranda@ufl.edu292 CGRC
Anthony BoullosaCytometry Core Research Technician III(352) 273-6032afbfl@ufl.edu292 CGRC
Doug SmithCTAC Manager(352) 392-1745dugsmith@ufl.edu