Cytometry

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Overview

Important COVID-19 news for ICBR Cytometry and CTAC Users:

    • ICBR Cytometry remains open to serve research deemed essential by UF Research.
    • User trainings on all instruments in CY and CTAC are now available.
    • We ask all users to have only the one person with the self-service reservation come into the core to operate the equipment- this helps with social distancing
    • We ask that all self service users wash their hands upon arrival to the core lab and put on gloves before touching computers or instruments.
    • We ask that all self service users clean the computer keyboard, mice and the instrument with sanitizing wipes and 70% ethanol when you are done running samples.
    • We ask that all sorting samples for drop off have ethanol resistant labels on the tubes and a written sample ID key for the Sorter operator.
    • We ask that our users please practice “social distancing” when setting up sorting’s with the CY staff.

The Cytometry core offers project consultingexperimental design and optimization as well as data analysis services to help researchers with large-scale and small-scale experiments acquired on our sorters and cytometry instruments. We coordinate closely with other ICBR cores (Next-Gen Sequencing, Gene Expression, Bioinformatics, Monoclonal Antibody, Electron Microscopy and Proteomics) on shared projects. Our core offers a full suite of services from staff assisted services to training courses for all users, as well as instrument training for 24/7 access to our self-service equipment usage.

The Flow Cytometry core provides a variety of tools and staff expertise for both live and fixed cell analysis. The laboratory incorporates numerous flow cytometers, from simple entry-level instruments to high-end 5-laser, 17-parameter instruments as well as a spectral cytometer to analyze your cells. We have  3 FACS ARIA’s and a SONY SH800 sorters to help users sort  your cells of interest. In addition, the laboratory offers a suite of microscopy equipment for self-service users. The microscopes are well equipped with a variety of excitation lines from near UV to red and can collect a variety of emission colors (depending on dye combinations), with viable cell time lapse and extensive computed parameter capabilities, including FRET, FRAP, image stitching, and 3D reconstruction. We offer a host of Microscope brands from a Leica SP5 Confocal, a Nikon Multiphoton Confocal system, a Nikon Live cell Imaging platform, 2 Olympus spinning Disk Confocal instruments, as well as our new Keyence BZX800.

 

Core News

UPDATE: ICBR Continues to Provide Support to UF Community UPDATE: ICBR Continues to Provide Support to UF Community
UPDATE: ICBR Continues to Provide Support to UF Community
With the progression of recent events, there are a lot of questions about how it affects ICBR users. Please see below, important changes that could impact your experience at ICBR. These changes will be in place for the foreseeable future. Please refer back to this page for timely updates. As always, check the UF dedicated site for campus-wide information and initiatives. LATEST NEWS ICBR is now open, Monday through Friday, to support research on the University of Florida campus. Please be...
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UPDATE: ICBR Continues to Provide Support to UF Community
Dr. Anna-Lisa Paul Appointed Director of UF ICBR Dr. Anna-Lisa Paul Appointed Director of UF ICBR
Dr. Anna-Lisa Paul Appointed Director of UF ICBR
The University of Florida Interdisciplinary Center for Biotechnology Research recently announced the appointment of its new director, Anna-Lisa Paul, Ph.D. Dr. Paul assumed the position effective March 20, 2020. For the past year, Dr. Paul has served as ICBR’s interim director. During that time, Dr. Paul spearheaded the center’s acquisition of state-of-the-art equipment and furthered ICBR’s collaborative relationship with UF Research departments. “The University of Florida Health Cancer Center h...
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Dr. Anna-Lisa Paul Appointed Director of UF ICBR
New Publication from Bioinformatics’ Tongjun Gu New Publication from Bioinformatics’ Tongjun Gu
New Publication from Bioinformatics' Tongjun Gu
Bioinformatics assistant scientist Tongjun Gu Ph.D. has developed a two-step logistic regression model for association studies where known factors can serve as controls. The model is helpful for studies seeking to identify novel regulators in the RNA editing process where the dominant enzyme was found in the first step and served as a control in the second step. Advantages of the model: It considered the sequencing depth in the analysis. It can be validated in the first step by the association ...
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New Publication from Bioinformatics’ Tongjun Gu
BI assistant scientist develops a tool for stratifying cancer patients BI assistant scientist develops a tool for stratifying cancer patients
BI assistant scientist develops a tool for stratifying cancer patients
Bioinformatics assistant scientist Tongjun Gu Ph.D. has developed an advanced deep learning method to stratify cancer patients into clinically relevant subgroups and further identify the potential biomarkers between the subgroups. This will help with the development of personalized treatments and prognosis. The method can integrate multi-platform datasets like gene expression, miRNA expression, methylation, copy number alteration, and protein expression. It can also do transfer learning between ...
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BI assistant scientist develops a tool for stratifying cancer patients
ICBR Proteomics & Mass Spectrometry (PM) aims to bring single-cell proteomics and metabolomics technologies to customers ICBR Proteomics & Mass Spectrometry (PM) aims to bring single-cell proteomics and metabolomics technologies to customers
ICBR Proteomics & Mass Spectrometry (PM) aims to bring single-cell proteomics and metabolomics technologies to customers
The cell is the basic unit of life. For multicellular organisms like animals and plants, each cell is different. Due to limited sensitivity, conventional ‘omic analyses require the input of tissues, organs or a mixture of thousands of cells, where information at the single-cell level has been diluted and averaged. This problem has severely limited our understanding of the intricate signaling and metabolic pathways and networks in different cells. In recent years, single-cell biology has become a...
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ICBR Proteomics & Mass Spectrometry (PM) aims to bring single-cell proteomics and metabolomics technologies to customers
Important Upcoming Billing Dates Important Upcoming Billing Dates
Important Upcoming Billing Dates
University of Florida and ICBR core labs will be officially closed during the UF holiday: Wednesday, December 25, through Wednesday, January 1. No samples or other packages can be accepted during this holiday closure time. Please coordinate with individual core laboratories for delivery of samples or other packages either before or after the UF winter break. Due to the winter break, ICBR’s upcoming billing schedule will be altered as follows: 12/16/2019 – Last invoices generated in 2019. ...
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Important Upcoming Billing Dates
A New Method to Examine Human Dopamine Transporter in Peripheral Blood A New Method to Examine Human Dopamine Transporter in Peripheral Blood
A New Method to Examine Human Dopamine Transporter in Peripheral Blood 
A team of UF neuroscientists led by Habibeh Khoshbouei, Ph.D., Pharm.D., Professor of Neuroscience and Psychiatry in the UF College of Medicine, has recently discovered a novel method using blood-borne immune cells to study the role of the neurotransmitter dopamine in the human brain.  This finding was reported in a publication titled ”A novel approach to study markers of dopamine signaling in peripheral immune cells” appearing in the Journal of Immunological Methods. While immunohistochemical a...
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A New Method to Examine Human Dopamine Transporter in Peripheral Blood
Dr. Tongjun Gu, from ICBR Bioinformatics, Improves Discovery Pipeline Dr. Tongjun Gu, from ICBR Bioinformatics, Improves Discovery Pipeline
Dr. Tongjun Gu, from ICBR Bioinformatics, Improves Discovery Pipeline
Bioinformatics assistant scientist Tongjun Gu Ph.D. has improved the pipeline for discovering ncRNA editing sites from four human cancers. Published in JCO Clinical Cancer Informatics, Clinical Relevance of Noncoding Adenosine-to-Inosine RNA Editing in Multiple Human Cancers, Dr. Gu’s research also presents multiple advanced statistical methods for differential and survival analysis. More information: Bioinformatics Services Contact Bioinformatics ...
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Dr. Tongjun Gu, from ICBR Bioinformatics, Improves Discovery Pipeline

Instruments

Staff

NameTitleTelephoneE-mailRoom
Andria DotyCytometry Scientific Director(352) 273-8186sun30@ufl.edu292B CGRC
Mariza MirandaCytometry Core Research Specialist II(352) 273-6032marizamiranda@ufl.edu292 CGRC
Anthony BoullosaCytometry Core Research Technician III(352) 273-6032afbfl@ufl.edu292 CGRC
Doug SmithCTAC Manager(352) 392-1745dugsmith@ufl.edu
Gary BrownCTAC Lab Technician(352) 273-6569gabrown@ufl.edu