Cytometry

ICBR-Cytometry@ad.ufl.edu   ///  (352) 273-8186   ///    RRID:SCR_019119 
Cytometers, Confocal Microscopes, Histology, In-Vivo Imaging & More

CORE OVERVIEW

Important COVID-19 news for ICBR Cytometry and CTAC Users:

    • We ask that all self service users wash their hands upon arrival to the core lab and put on gloves before touching computers or instruments.
    • We ask that all self service users clean the computer keyboard, mice and the instrument with sanitizing wipes and 70% ethanol when you are done running samples.
    • We ask that all sorting samples for drop off have ethanol resistant labels on the tubes and a written sample ID key for the Sorter operator.
    • We ask that our users please practice “social distancing” when setting up sorting’s with the CY staff.

The Cytometry core offers project consultingexperimental design and optimization as well as data analysis services to help researchers with large-scale and small-scale experiments acquired on our sorters and cytometry instruments. We coordinate closely with other ICBR cores (Next-Gen Sequencing, Gene Expression, Bioinformatics, Monoclonal Antibody, Electron Microscopy and Proteomics) on shared projects. Our core offers a full suite of services from staff assisted services to training courses for all users, as well as instrument training for 24/7 access to our self-service equipment usage.

The Flow Cytometry core provides a variety of tools and staff expertise for both live and fixed cell analysis. The laboratory incorporates numerous flow cytometers, from simple entry-level instruments to high-end 5-laser, 17-parameter instruments as well as a spectral cytometer to analyze your cells. We have  3 FACS ARIA’s and a SONY SH800 sorters to help users sort  your cells of interest. In addition, the laboratory offers a suite of microscopy equipment for self-service users. The microscopes are well equipped with a variety of excitation lines from near UV to red and can collect a variety of emission colors (depending on dye combinations), with viable cell time lapse and extensive computed parameter capabilities, including FRET, FRAP, image stitching, and 3D reconstruction. We offer a host of Microscope brands from a Leica SP5 Confocal, a Nikon Multiphoton Confocal system, a Nikon Live cell Imaging platform, 2 Olympus spinning Disk Confocal instruments, as well as our new Keyence BZX800.

CORE NEWS

UF ICBR Launches New Self-Service Timer UF ICBR Launches New Self-Service Timer
UF ICBR Launches New Self-Service Timer
UF | ICBR has launched their latest version of our self-service timer. Please see below, a guide on how the new timer can benefit your experience.   Accessing the Application To access the application, follow these steps: Navigate to selfservice.biotech.ufl.edu Click on Access the Self Service Suite button Authenticate with your GatorLink credentials Click on Timer Browsing the Cores To browse the list of cores, follow these steps: On the navigation bar on top of the screen, click on C...
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UF ICBR Launches New Self-Service Timer
UF Researcher Dr. Marco Salemi Utilizes ICBR Genomics Expertise to Understand COVID19 Epidemiology UF Researcher Dr. Marco Salemi Utilizes ICBR Genomics Expertise to Understand COVID19 Epidemiology
UF Researcher Dr. Marco Salemi Utilizes ICBR Genomics Expertise to Understand COVID19 Epidemiology
By Steven Madore, Ph.D. In recent years, public health emergencies caused by epidemics have led to the use of genome sequencing to identify and characterize viral pathogens. Rapid acquisition of high quality viral genomic sequences is critical for understanding viral pathogen origin, transmission and epidemiological spread. Ultimately deciphering the molecular characteristics of viruses accelerates the development of diagnostic assays and vaccine development and drug design and is important in u...
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UF Researcher Dr. Marco Salemi Utilizes ICBR Genomics Expertise to Understand COVID19 Epidemiology
UF researchers use AI datasets to track feral pigs, minimize disease risk UF researchers use AI datasets to track feral pigs, minimize disease risk
UF researchers use AI datasets to track feral pigs, minimize disease risk
Courtesy of UF/IFAS Written by Brad Buck  Feral pigs cost the agriculture industry at least $1.5 billion in damage, disease and control costs around the United States annually, running rampant on large swaths of grazing lands. The swine root in soil and eat most everything in sight. Farmers and ranchers will benefit from research by University of Florida scientists who are using artificial intelligence to gather data on feral hog reproduction and movement. As they roam grazing land, feral swine ...
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UF researchers use AI datasets to track feral pigs, minimize disease risk
UF | ICBR Scientists Contribute to Clinical Trial Testing Novel Treatment for Canine Atopic Dermatitis UF | ICBR Scientists Contribute to Clinical Trial Testing Novel Treatment for Canine Atopic Dermatitis
UF | ICBR Scientists Contribute to Clinical Trial Testing Novel Treatment for Canine Atopic Dermatitis
  Atopic dermatitis, or AD, is a common chronic inflammatory skin disease affecting dogs, causing defects of the skin barrier and modifications of the normal cutaneous microflora. While multiple treatment options are available, there is a growing interest in the use of alternative natural ingredient products. In human AD reports show the potential benefits of topical applications of heat-killed beneficial bacteria, with some studies demonstrating the efficacy of rapidly killed beneficial la...
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UF | ICBR Scientists Contribute to Clinical Trial Testing Novel Treatment for Canine Atopic Dermatitis
UF Researchers to Use AI to Predict How Hurricanes Spread Invasive Plants UF Researchers to Use AI to Predict How Hurricanes Spread Invasive Plants
UF Researchers to Use AI to Predict How Hurricanes Spread Invasive Plants
Courtesy of UF/IFAS Written by Brad Buck Scientists project hurricane intensity and frequency will increase with climate change. That leads researchers to want to better predict how storms will disperse and establish nonnative plant invaders. Knowing where invasive plants spread will always be useful. But it may become especially handy soon as the National Hurricane Center is considering moving the official start of the Atlantic hurricane season two weeks earlier, starting it May 15, instead of ...
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UF Researchers to Use AI to Predict How Hurricanes Spread Invasive Plants
World-Renowned UF Health Biochemistry Researcher Passes Away World-Renowned UF Health Biochemistry Researcher Passes Away
World-Renowned UF Health Biochemistry Researcher Passes Away
Courtesy of College of Medicine News University of Florida Health biochemistry professor Mavis Agbandje-McKenna, Ph.D., whose world-renowned work on the detailed structure of viruses led to advances in gene therapy treatments for different diseases, died Wednesday at her home near Gainesville of the neurodegenerative disease amyotrophic lateral sclerosis. She was 57. Agbandje-McKenna’s work with the small, infectious particles had a worldwide impact, allowing other scientists to be more precise ...
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World-Renowned UF Health Biochemistry Researcher Passes Away
UF Health Virologist Talks about Variants and the Future of the Coronavirus UF Health Virologist Talks about Variants and the Future of the Coronavirus
UF Health Virologist Talks about Variants and the Future of the Coronavirus
Courtesy of UF Health Written by Bill Levesque The novel coronavirus is doing what viruses do — in fact, what all life does. It’s mutating and creating new variants. This is evolution 101. Much of the discussion about the coronavirus in recent weeks has been about the potential impact of these variants on COVID-19 vaccines now being administered worldwide. Just what does the future hold? University of Florida Health virologist John Lednicky, Ph.D., a professor in the in the UF College of Public ...
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UF Health Virologist Talks about Variants and the Future of the Coronavirus
UF Health pilot program to deliver COVID-19 monoclonal antibody therapy to rural communities UF Health pilot program to deliver COVID-19 monoclonal antibody therapy to rural communities
UF Health pilot program to deliver COVID-19 monoclonal antibody therapy to rural communities
Courtesy of UF Health Written by Diana Tonnessen A University of Florida Health pilot program will bring a new COVID-19 monoclonal antibody therapy to rural communities in North Florida during the coming weeks. As part of the White House’s Operation Warp Speed initiative, the U.S. Department of Health and Human Services selected UF Health and the UF Clinical and Translational Science Institute to coordinate the distribution and administration of 1,000 doses of bamlanivimab, a monoclonal antibody...
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UF Health pilot program to deliver COVID-19 monoclonal antibody therapy to rural communities

CORE INSTRUMENTS

Cytometry