Cytometry

ICBR-Cytometry@ad.ufl.edu   ///  (352) 273-6032   ///    RRID:SCR_019119 
Cytometers, Confocal Microscopes, Histology, In-Vivo Imaging & More

CORE OVERVIEW

The ICBR Cytometry core provides investigators with access to Cytometry and Optical Microscopy instrumentation and services. We offer project consulting, with experimental design and optimization to help researchers plan both large and small-scale experiments utilizing our cell sorters, analyzers, small particle instruments and microscopes. Training is offered for most of our instruments with an eye towards creating knowledgeable, self-sufficient operators. We coordinate closely with other ICBR cores (Next-Gen Sequencing, Gene Expression, Bioinformatics, Monoclonal Antibody, Electron Microscopy, and Proteomics) on shared projects. Our core offers a large suite of services including staff-assisted instrument operation, cytometry theory and hands-on training courses, data analysis workstations, online analysis software licenses, and 24/7 self-service access to most of our equipment. Our cytometers, small particle instruments and microscopes are distributed between two buildings on the University of Florida’s Health Science Center campus, the Cancer Genetics Research Complex (CGRC) and the McKnight Brain Institute (MBI).

The Cytometry core provides a variety of tools and staff expertise for analyzing and imaging live cells, fixed cells, tissue sections, and small particles (i.e.: like EVs, exosomes, liposomes). The laboratory incorporates numerous flow cytometers, from simple entry-level analyzers to high-end 5-laser, 28-parameter instruments to analyze and sort cells. We have sorters to help users collect cells of interest: BD Symphony S6, BD Fusion, and a Sony SH800. Our collection of analyzing cytometers includes several traditional filter-based systems (Cytoflex LX, Fortessa, Accuri, Canto, Symphony A3) as well as spectral analyzers (Sony SP6800 and Cytek Aurora). For those who are working with small particles, we also have a NanoFCM and a NanoSight NS300.

In addition, the core maintains an assortment of microscopes, in both confocal and widefield formats. The microscopes are well equipped to work with a variety of fluorescent labels from near UV out to long-red. Within in our assortment of imaging capabilities are a number of advanced functions, including FLIM, FRET, FRAP, image stitching, 3D processing, and in-vitro time-lapse imaging. We offer a range of microscope models including our White-Light Laser equipped Leica Stellaris 8 spectral confocal, a Leica SP5 spectral confocal, and two Olympus IX81-DSU spinning disk confocals. Our collection of widefield microscopes includes a Keyence BZ-X800, and a Nikon Ti-E equipped for live-cell imaging.

CORE NEWS

Stars Align for Science: University of Florida and NSF NRAO Forge Path for Discovery Stars Align for Science: University of Florida and NSF NRAO Forge Path for Discovery
Stars Align for Science: University of Florida and NSF NRAO Forge Path for Discovery
Stars Align for Science: University of Florida and NSF NRAO Forge Path for Discovery The U.S. National Science Foundation National Radio Astronomy Observatory (NSF NRAO) and the University of Florida (UF) have announced a dynamic new partnership to advance science education and public outreach at a future Next Generation Very Large Array (ngVLA) antenna site in Florida. This collaboration, signed with a Memorandum of Understanding (MOU) at the debut of the ngVLA prototype antenna in New Mexico l...
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Stars Align for Science: University of Florida and NSF NRAO Forge Path for Discovery
Florida Trend: Seeding Exploration Florida Trend: Seeding Exploration
Florida Trend: Seeding Exploration
Seeding Exploration Twelve years to get 12 grams. Twelve years of research and planning, of conducting experiments with simulated material, of traveling to the world’s most inhospitable environments. Twelve years of writing proposals and being told no. University of Florida research scientists Rob Ferl and Anna-Lisa Paul weren’t asking NASA for just 12 grams oflunar regolith – basically dust and dirt from the moon – but that was the payoff in 2022 for their perseverance. ...
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Florida Trend: Seeding Exploration
Sony ID7000 Spectral Cytometer at UF ICBR Sony ID7000 Spectral Cytometer at UF ICBR
Sony ID7000 Spectral Cytometer at UF ICBR
Sony ID7000 Spectral Cytometer at UF ICBR The Cytometry & Optical Microscopy core at UF ICBR has recently acquired the state-of-the-art Sony ID7000 Spectral Cytometer, located at McKnight Brain Institute (MBI) room L3-160. This newest addition to the facility offers unparalleled capabilities for researchers, significantly expanding their experimental possibilities. The Sony ID7000 features an impressive configuration of five lasers and 144 detectors, enabling experiments utilizing over 40 fl...
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Sony ID7000 Spectral Cytometer at UF ICBR
ICBR Billing Schedule Update: Key Dates for Fiscal Year End 2025 ICBR Billing Schedule Update: Key Dates for Fiscal Year End 2025
ICBR Billing Schedule Update: Key Dates for Fiscal Year End 2025
Please note: Due to the Fiscal Year End, the last invoices to process for FY2025 will be created on Monday, June 9th.  ICBR’s upcoming billing schedule will be altered as follows: 6/9/2025 – Last invoices generated in fiscal year 2025 6/16/2025 at noon – Deadline to submit disputes for FY2025 payment. 6/30/2025 – Invoices will begin to be created for FY2026 If you have expiring funds, please work with your core laboratory contact to make sure your project is completed and billed before the 06/...
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ICBR Billing Schedule Update: Key Dates for Fiscal Year End 2025
STED super resolution imaging is now available to UF researchers STED super resolution imaging is now available to UF researchers
STED super resolution imaging is now available to UF researchers
STED super resolution imaging is now available to UF researchers ICBR’s Leica Stellaris 8 laser scanning spectral confocal microscope has been relocated from the CGRC building to its new home in ICBR Cytometry’s Optical Imaging suite in the McKnight Brain Institute (MBI room LG-164).  During reassembly, the instrument was upgraded with Leica’s STED super resolution technology. STED (STimulated Emission Depletion) offers the ability to dramatically improve fluorescent image resolution. The new ST...
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STED super resolution imaging is now available to UF researchers
UF ICBR Closed for Martin Luther King, Jr. Day UF ICBR Closed for Martin Luther King, Jr. Day
UF ICBR Closed for Martin Luther King, Jr. Day
UF ICBR Closed for Martin Luther King, Jr. Day UF ICBR will be closed on Monday, January 20h in observation of Martin Luther King, Jr. Day. Normal hours will resume on Tuesday, January 21st. No packages will be accepted during this time! ...
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UF ICBR Closed for Martin Luther King, Jr. Day
ICBR Closed for the Holidays! ICBR Closed for the Holidays!
ICBR Closed for the Holidays!
Wishing you and yours a Happy Holiday and a joyful New Year. Best wishes from your friends at UF | ICBR! ICBR will be closed beginning at noon on Tuesday, December 24th through Wednesday, January 1st. We here at ICBR are thankful for you, and hope you enjoy the holiday! And a special thank you the near thousand users that came through our doors this year, including 300+ PIs from over a hundred UF departments! We look forward to working with you 2025!     Normal business hours will resu...
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ICBR Closed for the Holidays!
ICBR Closed for the Thanksgiving Holiday! ICBR Closed for the Thanksgiving Holiday!
ICBR Closed for the Thanksgiving Holiday!
Wishing you and yours a joyous Thanksgiving. Best wishes from your friends at UF | ICBR! ICBR will be closing early Wednesday afternoon at 3pm, and will be closed all day Thursday and Friday (November 28th and 29th); returning on Monday (December 2nd). We here at ICBR are thankful for you, and hope you enjoy the holiday! And a special thank you the near thousand users that came through our doors this year, including 300+ PIs from over a hundred UF departments! We look forward to working with you...
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ICBR Closed for the Thanksgiving Holiday!

CORE INSTRUMENTS

Cytometry