The Cytek Aurora spectral flow cytometer uses spectral technology to optimize sensitivity and enhance dim signal detection. This 3-laser system can detect the full spectral emission of each fluorochrome off each laser excitation, accommodating panels with up to 24 colors ranging from BV421 to APC-Fire 810. Spectral unmixing works well for single color assays to high parameter panels due to less photon counting errors, tighter populations, and autofluorescence extraction making difficult high autofluorescence tissues/cells easier to analyze.