Illumina MiSeq System

UF | ICBR NextGen DNA Sequencing

Access focused applications such as targeted resequencing, metagenomics, small genome sequencing, targeted gene expression profiling, and more. MiSeq reagents enable up to 15 Gb of output with 25 million sequencing reads and 2 × 300 bp read lengths.

The Illumina MiSeq System offers capabilities for small whole-genome sequencing, amplicon sequencing, gene expression profiling, small RNA analysis and 16s metagenomic sequencing in a compact instrument. Using a high throughput kit, MiSeq can produce as much as 15 Gb of output from 50 million paired end reads (at a read length of 300 bp.) The system also features an intuitive touch screen interface, plug-and-play reagent cartridges with RFID tracking, on-screen video tutorials and step-by-step guides throughout each sequencing workflow. The Illumina sequencing systems utilize a well-established sequencing by synthesis (SBS) method in which fluorescently labeled nucleotide bases are detected as they are incorporated into DNA template strands. All four reversible terminator-bound dNTPs are present in each sequencing cycle and this feature minimizes bias of incorporation and greatly reduces raw error rates.


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