Monoclonal Antibody

Services & Fees
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Offered Services

Microplate Reader Self-Service (per hour)

Walk-up, self-service use of the SpectraMax M5 system (a five-mode multi-detection microplate reader system that offers PathCheck Pathlength Measurement Technology with ready-to-read protocols) or the BioTek Synergy H4 Multimode system (all-in-one Hybrid microplate reader).

Consultation

For NEW customers and NEW projects: Schedule a consultation to discuss custom project needs and prepare a project outline with an ICBR staff member.

Monoclonal Antibody Development

The first two phases of monoclonal antibody development (immunization and fusion) are included in this request; the next phase (cloning) must be requested separately. Required information includes: intended antibody applications, antigen details, and screening plans.

Cloning

Single cell cloning of uncloned hybridoma cell line from fusion. Project will include: deposit of two monoclonal cell lines (primary and secondary), isotyping of primary cell line, mycoplasma testing of primary cell line. ICBR will screen supernatants from single cell wells by ELISA at an additional cost.

Production

In vitro production of monoclonal antibodies from cloned hybridoma cell lines. Both CL-350 (high density) and standard culture flasks (supernatant volumes range from 50 mls to 1000 mls) are available. Cells will be cultured in medium designed to support monoclonal antibody production. The medium will be supplemented with low IgG containing fetal bovine serum.

Purification

Monoclonal antibodies produced from hybridoma cell lines will be purified using affinity chromatography. Purification scale is based on antibody affinity resin binding capacity (small, standard, and large equate to 2, 5, and 10 mL).

Small scale will be used for hybridoma supernatants from standard culture flasks in volumes up to 250 mls.

Standard scale will be used for CL-350 (high density) supernatants or hybridoma supernatants from standard culture flasks in volumes greater than 250 mls.

Large scale will be used for high level antibody secreting hybridoma cell lines.

Purification services are also available for polyclonal antibodies from serum.

Deposit Cell Lines

Includes freezing 4 vials of hybridoma cells in 2 separate self-filling LN2 freezers. All cell lines accepted for deposit must be tested for mycoplasma contamination, unless cells are received directly from repositories that certify cells are mycoplasma free.

Direct Detect Training

Training session is approximately 30 minutes. Up to three trainees may participate in the same session.

Microplate Reader Training

Training session is approximately 30 minutes. Up to three trainees may participate in the same session.

Octet Service

Octet minimum session is 2 hours. Customer brings all consumables and supplies including biosensors.

Octet Training

Training session is approximately 2 hours. Customer may provide their own reagents if QC standards are met; otherwise example reagents will be provided by the core. All sensors must be supplied by the customer.

ProteinSimple Wes Service

Customer supplies all reagents and consumables including the Protein Simple 13 or 25 capillary cartridges.

ProteinSimple Wes Training

Automated Western training for self-service users; consumable cartridges and reagents are not included.

Synergy H4 Training

Training session is approximately 30 minutes.

Introduction to Mammalian Cell Culture Techniques

This workshop is designed for those new to mammalian cell culture or needing a refresher course on the basics. The workshop includes informal lectures and hands-on experience. The major topics are: maintaining mammalian cells in culture (aseptic technique, thawing, counting, expanding, freezing) and troubleshooting (rescuing sick cells and recognizing and dealing with contamination).

This two-day workshop is offered twice a year. The minimum number of registrants is 4.

Service Price List

Biotinylation

ServicesUFNon-ProfitCommercialDetails
Biotinylation $92$155.25$207Biotinylation of purified antibodies that includes biotin incorporation assay. Standard scale up to 5 mgs.

Cloning

ServicesUFNon-ProfitCommercialDetails
Cloning$400$600$800Single cell cloning of uncloned hybridoma cell line from fusion. Service will include: deposit of two monoclonal cell lines (primary and secondary), isotyping of primary cell line, mycoplasma testing of primary cell line. The Monoclonal Antibody Core will screen supernatants from single cell wells by ELISA for an additional fee.
Cloning (deposit 3 additional vials)$90$135$175
Cloning (freeze extra vials) $30$45$60Freezing of additional vials of primary and/or secondary clone. Price per vial.
Cloning (no positives after primary screen) $306$381$400Sufficient outgrowth of single colony wells, no antibody positives.
Cloning (No positives after secondary screen) $385$460$480
Cloning (No positives after tertiary screen) $387$514$540
Cloning (second cell line in parallel) $400$400$400Single cell cloning of second cell line in parallel with another cell line on the same day; deposit of up to two monoclonal cell lines.

Consultation

ServicesUFNon-ProfitCommercialDetails
Consultations, Training & Assistance (per hour) $35$40$45

Deposit

ServicesUFNon-ProfitCommercialDetails
Deposit Hybridoma Line Produced Outside ICBR $138$195.50$230This service includes producing 4 vials for deposit. The price does not include cryo-storage or mycoplasma testing.
Isotyping$34$44$52
Mycoplasma Testing: Sample #1 $80.50$115$149.50Mycoplasma Testing using Lonza Mycoalert kit and controls.
Mycoplasma Testing: Samples #2-4 $23$34.50$46Mycoplasma Testing using Lonza Mycoalert kit and controls. Samples #2-4 must run in parallel and on same day as Sample #1.

ELISA

ServicesUFNon-ProfitCommercialDetails
ELISA Screening $40$60$6596 well plate format. Price per plate.

Fusion

ServicesUFNon-ProfitCommercialDetails
Cell Fusion $850$1300$1700
Cell Fusion (No Positive After Primary Screen) $500$550$600Cell Fusion with good outgrowth of hybridomas and no antibody positives.
Cell Fusion (No Positive After Secondary Screen) $680$748$816
Cell Fusion (No Positive After Tertiary Screen) $800$880$960

Immunization

ServicesUFNon-ProfitCommercialDetails
Adjuvant CpG (10 ug) $14$15$16Adjuvant CpG, per 10 ul.
Animal Weekend VetTech Checks (Per 2 Cages) $11.53$11.53$11.53
Housing Mice$29$30$31Monthly housing charge after the first 6 months. Price per cage.
Immunization Fee $20$22$25
Mouse$30$30$30
ACS Mouse Order Standard Fee $46$50$50
MPL + TDM Adjuvant (0.1 mL) $14.30$16$17MPL + TDM Emulsion (Sigma)
Ordering Fee$10$11$15
Serum Titer Bleed$20$22$25

Octet

ServicesUFNon-ProfitCommercialDetails
Octet QKe Full Service-charged per hour (2 hour minimum) $23$40.25$57.50Does not include sensors or reagents/supplies; time cannot be split and does not rollover to multiple days.
Octet Quantitation $10$15$20IgG subclass; Includes sensors. Price per sample.

Octet Services

ServicesUFNon-ProfitCommercialDetails
Octet Self-Service per hour (2 hour minimum) $15$30$40This includes the Qke buffer and plate, gloves, and pipette tips

Plate Reader

ServicesUFNon-ProfitCommercialDetails
Plate Reader Use$13.80$13.80$13.80Price per hour.

Production

ServicesUFNon-ProfitCommercialDetails
CL 350: 3 harvests$425$500$600
CL 350: extended run (> 3 harvests) (per 3 harvests) $140$154$161Includes anyting > 3 harvests. Price per harvest.
CL 350: extended run (> 3 harvests) Flasks # 2-4 run in parallel with 1st flask (NP & COM) $0.00$40.25$40.25Monoclonal antibody production Cel-Line high density extended run; > 3 harvests. Flasks #2-4 must be run in parallel with flask #1.
Monoclonal antibody production hybridoma supernatant 100 mls$80.50$143.75$195.50Cell culture supernatant up to 100 mls.
Monoclonal antibody production hybridoma supernatant 1000 mls $371$408$427Cell culture supernatant up to 1000 mls.
Monoclonal antibody production hybridoma supernatant 250 mls$140$166.75$212.75Cell culture supernatant up to 250 mls.
Monoclonal antibody production hybridoma supernatant 50 mls $65$78.20$92Cell culture supernatant up to 50 mls
Monoclonal antibody production hybridoma supernatant 500 mls $230.50$254$265Cell culture supernatant up to 500 mls.

Protein Quantitation

ServicesUFNon-ProfitCommercialDetails
BCA Assay $50$75$100
Direct Detect Protein Quantitation $11.50$13.80$16.10Analyze proteins using Millipore Direct Detect Infrared Spectrometer. Price per card.

Purification

ServicesUFNon-ProfitCommercialDetails
Large scale purification$450$550$650Large scale purification using 10.0ml protein G column prepared from bulk matrix.
Purification $375$475$575Standard scale purification using 5.0ml protein G column prepared from bulk matrix.
Small Scale Purification $150$250$350Small scale purification using 1.0-2.0 ml protein G column prepared from bulk matrix.
Tandem Purification $75$175$200Tandem purification run on used column.

Shipping

ServicesUFNon-ProfitCommercialDetails
Dry Ice per 5lb $6.50$7$15
Handling Charges (Includes Shipping Container) $30$45$50

Spleen

ServicesUFNon-ProfitCommercialDetails
Spleen Harvest$75$125$100Includes removing spleen, processing, counting, and cryopreserving.

Storage

ServicesUFNon-ProfitCommercialDetails
Cryo-Storage Retrieval $30$75$75
Cryostorage Monthly Fee $23$23$23to be paid monthly for storage
Freeze Uncloned Hybridomas$28.75$46$63.25Limit of 2 vials per cell line. Price per cell line.
Store Uncloned Hybridomas$23$23$23Annual fee for storage of uncloned hybridoma cell lines. Price per cell line.

Subculture

ServicesUFNon-ProfitCommercialDetails
Freeze 4 Extra CryoVials Per Line $30$40$50
Subculture ICBR Produced Hybridoma Cells (includes 1 cryovial for the customer) $34.50$69$80.50Subculture of hybridoma cells; t.25 flask.

Supplies & Reagents

ServicesUFNon-ProfitCommercialDetails
Kits, supplies, reagents (per unit) $11.50$11.50$11.50
Ordering Fee$10$11$15

Western

ServicesUFNon-ProfitCommercialDetails
Traditional Western Blot Labor $90$100$105
Wes Equipment Cost Per Run$40$44$46