Monoclonal Antibody

Services & Fees
MONOCLONAL ANTIBODY:     Home   |   Staff & Contact   |  Services  |   Instruments  |  Core Resources  |  Reagents

UF ICBR’s billing and service requests are powered by Agilent CrossLab. Ready to request a service? Log into CrossLab and submit a submission ID. New to CrossLab? Click here to become a customer

Offered Services

Microplate Reader Self-Service (per hour)

Walk-up, self-service use of the SpectraMax M5 system (a five-mode multi-detection microplate reader system that offers PathCheck Pathlength Measurement Technology with ready-to-read protocols) or the BioTek Synergy H4 Multimode system (all-in-one Hybrid microplate reader).

Consultation

For NEW customers and NEW projects: Schedule a consultation to discuss custom project needs and prepare a project outline with an ICBR staff member.

Monoclonal Antibody Development

The first two phases of monoclonal antibody development (immunization and fusion) are included in this request; the next phase (cloning) must be requested separately. Required information includes: intended antibody applications, antigen details, and screening plans.

Cloning

Single cell cloning of uncloned hybridoma cell line from fusion. Project will include: deposit of two monoclonal cell lines (primary and secondary), isotyping of primary cell line, mycoplasma testing of primary cell line. ICBR will screen supernatants from single cell wells by ELISA at an additional cost.

Production

In vitro production of monoclonal antibodies from cloned hybridoma cell lines. Both CL-350 (high density) and standard culture flasks (supernatant volumes range from 50 mls to 1000 mls) are available. Cells will be cultured in medium designed to support monoclonal antibody production. The medium will be supplemented with low IgG containing fetal bovine serum.

Purification

Monoclonal antibodies produced from hybridoma cell lines will be purified using affinity chromatography. Purification scale is based on antibody affinity resin binding capacity (small, standard, and large equate to 2, 5, and 10 mL).

Small scale will be used for hybridoma supernatants from standard culture flasks in volumes up to 250 mls.

Standard scale will be used for CL-350 (high density) supernatants or hybridoma supernatants from standard culture flasks in volumes greater than 250 mls.

Large scale will be used for high level antibody secreting hybridoma cell lines.

Purification services are also available for polyclonal antibodies from serum.

Deposit Cell Lines

Includes freezing 4 vials of hybridoma cells in 2 separate self-filling LN2 freezers. All cell lines accepted for deposit must be tested for mycoplasma contamination, unless cells are received directly from repositories that certify cells are mycoplasma free.

Direct Detect Training

Training session is approximately 30 minutes. Up to three trainees may participate in the same session.

Microplate Reader Training

Training session is approximately 30 minutes. Up to three trainees may participate in the same session.

Octet Service

Octet minimum session is 2 hours. Customer brings all consumables and supplies including biosensors.

Octet Training

Training session is approximately 2 hours. Customer may provide their own reagents if QC standards are met; otherwise example reagents will be provided by the core. All sensors must be supplied by the customer.

ProteinSimple Wes Service

Customer supplies all reagents and consumables including the Protein Simple 13 or 25 capillary cartridges.

ProteinSimple Wes Training

Automated Western training for self-service users; consumable cartridges and reagents are not included.

Synergy H4 Training

Training session is approximately 30 minutes.

Introduction to Mammalian Cell Culture Techniques

This workshop is designed for those new to mammalian cell culture or needing a refresher course on the basics. The workshop includes informal lectures and hands-on experience. The major topics are: maintaining mammalian cells in culture (aseptic technique, thawing, counting, expanding, freezing) and troubleshooting (rescuing sick cells and recognizing and dealing with contamination).

This two-day workshop is offered twice a year. The minimum number of registrants is 4.

Service Price List

Biotinylation

Cloning

Consultation

Deposit

ELISA

Fusion

Immunization

Octet

Octet Services

Plate Reader

Production

Protein Quantitation

Purification

Shipping

Spleen

Storage

Subculture

Supplies & Reagents

Western