Proteomics & Mass Spectrometry

Services & Fees
PROTEOMICS & MASS SPECTROMETRY:     Home   |   Staff & Contact   |  Services  |   Instruments  |   Core Resources

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Offered Services

MRM and "Mass Western" Analysis

MRM quantitative analysis (relative and absolute quantification) of small molecules and peptides/proteins. Price per sample for full service.

MRM Training and Self Service

Two-day training to learn MRM optimization to quantify small molecules and peptides. Price per hour for self service.

Luminex Biomarker Assay

Luminex biomarker immunoassays are antibody-based and there are customized panels for multiplex protein quantitation using the Luminex instrument platform. High-throughput screening up to 500 biomarkers simultaneously.

Luminex Training and Self Service

Protein Identification

Routine protein identification by tandem mass spectrometry from 1D gel bands, 2D gel spots, solution, and immunoprecipitates.

Quantitative Proteomics

Quantitative proteomics analyses. Specific methodology varies with the nature of the sample and the goals of the project.

Post-translational Modification Analysis

Mapping of post-translational modifications or derivatives (e.g. phosphorylation, ubiquitination, acetylation).

Custom Project

Schedule a consultation to discuss custom project needs and prepare statement of work with the Proteomics and Mass Spectrometry team.

1D and 2D Gel Analysis

Analysis includes 1D and 2D gel, 2D DIGE, image analysis, staining, gel excision, and Western Blot.

1D and 2D Gel Instrument Training and Self Service

Sample Fractionation

Separation of proteins and peptides by 3100 OFFGEL Fractionator or HPLC.

Workshop and Training

Workshops

  • Quantitative Proteomics
  • Data Analysis

Training per request

  • Sample preparation
  • HPLC Training and Self Service
  • Typhoon Scanner Training and Self Service

Service Price List

Last Updated: 08/2019

Direct Infusion

ServicesUFNon-ProfitCommercialDetails
Direct infusion $15$25$40Service includes directly infusing pure samples to mass spectrometer; no liquid chromatography separation.

Gel-related services

ServicesUFNon-ProfitCommercialDetails
1D PAGE mini, 7x8 cm$75$112$150Service includes 1- and  2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot.
1D PAGE mini, 7x8 cm with electroblot $90$130$180Service includes 1- and  2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot
1D PAGE sample prep per protein sample $5$7.5$10Service includes 1- and  2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot.
1D PAGE, 16x16 cm $125$185$250Service includes 1- and  2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot.
1D PAGE, 16x16 cm with Electroblot $185$277$370Service includes 1- and  2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot.
2D PAGE DIGE per gel $600$800$1000Service includes 1- and  2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot.
2D PAGE extra large, 24x24 cm $225$337$450Service includes 1- and  2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot.
2D PAGE extra large, 24x24 cm with blot $225$337$450Service includes 1- and  2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot.
2D PAGE medium, 16x16 cm $160$235$300Service includes 1- and  2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot.
2D PAGE medium, 16x16 cm with blot $185$375$500Service includes 1- and  2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot.
2D PAGE mini, 7x8 cm $110$160$200Service includes 1- and  2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot.
2D PAGE mini, 7x8 cm with blot $125$180$225Service includes 1- and  2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot.
2D PAGE Sample Prep per set of raw samples $130$185$240Service includes 1- and  2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot.
2D PAGE Spot detection per gel $70$105$140Service includes 1- and  2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot.
DeCyder image analysis per hour $60$90$120Service includes 1- and  2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot.
Excise protein gel plug, manual, per spot$5$7.35$10Service includes 1- and  2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot.
Excise protein gel plug, ProPic, price per plate $40$60$80Service includes 1- and  2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot.
IEF, Native agarose for large molecules per plate $150$225$300Service includes 1- and  2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot.
IEF, Native PAGE $100$150$200Service includes 1- and  2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot.
IEF, Native PAGE with electroblot $120$180$240Service includes 1- and  2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot.
Preparative IEF using 3100 OFFGEL fractionator $150$200$300Service includes 1- and  2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot.
Preparative SDS PAGE using mini prep cell $200$450$600Service includes 1- and  2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot.
Protein stain, Colloidal blue stain per 50 ml $10$15$20Service includes 1- and  2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot.
Protein stain, Deep purple stain per 50 ml$25$35$50Service includes 1- and  2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot.
Protein stain, ProQ Diamond Phosphoprotein stain per 50 ml $50$75$100Service includes 1- and  2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot.
Protein stain, ProQ Emerald glycoprotein stain per 50 ml $60$90$110Service includes 1- and  2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot.
Protein stain, SilverQuest stain per 50 ml $15$22$33Service includes 1- and  2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot.
Protein stain, Sypro Ruby stain per 50 ml $25$35$50Service includes 1- and  2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot.
PVDF Membrane $40$56$70Service includes 1- and  2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot.
SameSpot image analysis per hour $80$100$120Service includes 1- and  2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot.
SameSpot self service image analysis per hour $60$75$90Service includes 1- and  2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot.
Self service-1D PAGE mini, 7x8 cm$15$23$30
Self service-2D PAGE DIGE per gel $300$400$500
Self service-2D PAGE extra large, 24x24 cm $150$225$300
Self service-2D PAGE medium, 16x16 cm $80$120$150
Self service-2D PAGE mini, 7x8 cm $50$75$90
Self service-2D PAGE Spot detection per gel (Typhoo scanner) $25$40$50
Self service-Blot PVDF Membrane $25$35$45
Self service-DeCyder image analysis per hour $40$60$80
Self service-Excise protein gel plug, manual, per spot $1$1.50$2
Self service-Excise protein gel plug, ProPic, price per plate $20$30$40
Self service-IEF, Native PAGE $30$45$60
Self service-Preparative IEF using 3100 OFFGEL fractionator $50$90$120
Self service-Preparative SDS PAGE using mini prep cell $150$225$300
Self service-Protein stain, Colloidal blue stain per 50 ml $10$15$20
Self service-Protein stain, Deep purple stain per 50 ml $25$35$50
Self service-Protein stain, ProQ Diamond Phosphoprotein stain per 50 ml $50$75$100
Self service-Protein stain, ProQ Emerald glycoprotein stain per 50 ml $60$90$110
Self service-Protein stain, SilverQuest stain per 50 ml $15$22$33
Self service-Protein stain, Sypro Ruby stain per 50 ml$25$35$50
Special project, per sample $150$225$300Service includes 1- and  2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot.
Typhoon self service per scan$25$37.50$50Service includes 1- and  2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot.

Instrumental analysis

ServicesUFNon-ProfitCommercialDetails
HPLC (SCX, SE), per sample $100$150$200Sample analysis on HPLC, LCMS instruments.
LC-MS/MS - 1 h gradient for protein ID, per sample $105$168$262.50Sample analysis on HPLC, LCMS instruments.
LC-MS/MS - 1 h gradient for protein ID, per sample if > 50 $70$116.20$199.50Sample analysis on HPLC, LCMS instruments.
LC-MS/MS - 1 h gradient for protein ID, per sample if > 50, Orbitrap-Fusion$90$149.40$220.50
LC-MS/MS - 1 h gradient for protein ID, per sample if >10$90$135$220.50Sample analysis on HPLC, LCMS instruments.
LC-MS/MS - 1 h gradient for protein ID, per sample if >10, Orbitrap-Fusion $110$165$242
LC-MS/MS - 1 h gradient for protein ID, per sample, Orbitrap-Fusion $125$187.50$290
LC-MS/MS - 1 h gradient for small molecule, per sample, Orbitrap-Fusion with high flow UPLC system$205$307.50$410
LC-MS/MS - 2 hr gradient for protein ID, per sample $176$281.60$383.68Sample analysis on HPLC, LCMS instruments.
LC-MS/MS - 2 hr gradient for protein ID, per sample if > 10 QE Plus$126$189$292.32Sample analysis on HPLC, LCMS instruments.
LC-MS/MS - 2 hr gradient for protein ID, per sample if > 10, Orbitrap-Fusion $146$219$292
LC-MS/MS - 2 hr gradient for protein ID, per sample if > 5 $151$226.50$332.20Sample analysis on HPLC, LCMS instruments.
LC-MS/MS - 2 hr gradient for protein ID, per sample if > 5, Orbitrap-Fusion $171$256.50$352.26
LC-MS/MS - 2 hr gradient for protein ID, per sample if > 50 QE Plus $111$166.50$251.97Sample analysis on HPLC, LCMS instruments.
LC-MS/MS - 2 hr gradient for protein ID, per sample if > 50, Orbitrap-Fusion$121$181.50$254.10
LC-MS/MS - 2 hr gradient for protein ID, per sample, Orbitrap-Fusion $196$313.60$401.80
LC-MS/MS - 3 hr gradient for protein ID, per sample if > 10 Orbtrap Fusion $210$315$420
LC-MS/MS - 3 hr gradient for protein ID, per sample if > 10 QE Plus $170$255$340
LC-MS/MS - 3 hr gradient for protein ID, per sample if > 5 Orbtrap Fusion$240$360$480
LC-MS/MS - 3 hr gradient for protein ID, per sample if > 5 QE Plus$220$330$440
LC-MS/MS - 3 hr gradient for protein ID, per sample if > 50 Orbtrap Fusion $190$315.40$380
LC-MS/MS - 3 hr gradient for protein ID, per sample if > 50 QE Plus $160$240$320
LC-MS/MS - 3 hr gradient for protein ID, per sample Orbtrap Fusion $270$432$540
LC-MS/MS - 3 hr gradient for protein ID, per sample QE Plus $250$400$500
LC-MS/MS - 4 hr gradient for protein ID, per sample if > 10 Orbtrap Fusion $260$390$520
LC-MS/MS - 4 hr gradient for protein ID, per sample if > 5 Orbtrap Fusion$290$435$580
LC-MS/MS - 4 hr gradient for protein ID, per sample if > 50 Orbtrap Fusion $240$360$480
LC-MS/MS - 4 hr gradient for protein ID, per sample Orbtrap Fusion $320$480$640
LC-MS/MS - gel spot or band - 20 min gradient per sample $82$131.20$164Sample analysis on HPLC, LCMS instruments.
LC-MS/MS - gel spot or band - 20 min gradient per sample if > 10 $57$85.50$122.55Sample analysis on HPLC, LCMS instruments.
LC-MS/MS - gel spot or band - 20 min gradient per sample if in house$47$75.20$110.92Sample analysis on HPLC, LCMS instruments.
LC-MS/MS - gel spot or band - 20 min gradient per sample, Orbitrap-Fusion $102$163.20$183.60
LC-MS/MS - gel spot or band - 20 min gradient, per sample if > 10, Orbitrap-Fusion$77$115.50$141.68
LC-MS/MS - gel spot or band - 20 min gradient, per sample if >50$37$55.50$86.21Sample analysis on HPLC, LCMS instruments.
LC-MS/MS - gel spot or band - 20 min gradient, per sample if >50, Orbitrap-Fusion $57$85.50$106.02
LC-MS/MS-1 h gradient for protein ID, per sample if > 50 LTQ-Orbitrap $35$52.50$70
LC-MS/MS-1 h gradient for protein ID, per sample if >10 LTQ-Orbitrap $55$82.50$110
LC-MS/MS-1 h gradient for protein ID, per sample LTQ-Orbitrap $65$104$130
LC-MS/MS-2 hr gradient for protein ID, per sample if > 10 LTQ-Orbitrap $96$144$192
LC-MS/MS-2 hr gradient for protein ID, per sample if > 5 LTQ-Orbitrap $116$174$232
LC-MS/MS-2 hr gradient for protein ID, per sample if > 50 LTQ-Orbitrap $71$106.50$146.26
LC-MS/MS-2 hr gradient for protein ID, per sample LTQ-Orbitrap $126$201.60$252
LC-MS/MS-gel spot or band-20 min gradient, per sample if >50 LTQ-Orbitrap $28$42$58.80
LC-MS/MS-gel spot or band-20min gradient per sample if > 10 LTQ-Orbitrap $30$45$63
LC-MS/MS-gel spot or band-20min gradient per sample if in house LTQ-Orbitrap $25$41.50$53.75
LC-MS/MS-gel spot or band-20min gradient per sample LTQ-Orbitrap $32$53.12$68.80
MALDI - TOF MS per sample $35$50$125Sample analysis on HPLC, LCMS instruments.

Luminex Services

MRM Quantitative Services

ServicesUFNon-ProfitCommercialDetails
4000 QTRAP MRM Self-Service $35$50.75$59.50MRM quantitative analysis of peptides and small molecules. Price per hour.
MRM-TSQ Altis$105$157.50$210

Method development

ServicesUFNon-ProfitCommercialDetails
Build selected ion acquisition method $300$450$600
Data Search (add SILAC, PTM) $60$90$120
Method development for special projects, per hour $60$100$200Method development service for special projects.

Protein Identification

ServicesUFNon-ProfitCommercialDetails
Additional data search /sample $20$30$40Additional data search /sample

Quantitative Analysis

ServicesUFNon-ProfitCommercialDetails
iTRAQ data search$320$480$560
TMT Data Search $400$600$800

Quantitative Proteomics

ServicesUFNon-ProfitCommercialDetails
iodoTMT-6 plex $1600$2400$3200
MRM-4000 QTRAP, per sample $45$65$105
MRM-Method development (per hour) $60$100$200
PRM-Orbitrap fusion, per sample $400$480$800
PRM-QE plus, per sample $350$420$700
Quantitative Proteomic-Label Free $200$300$400Set up fee for 1 experiment (2 to 4 samples).
Quantitative proteomics-iTRAQ-8 plex Analysis$3130$4695$6260(Up to 8 samples, 14 fractions, including LC-MS/MS and 1 data search)
Quantitative proteomics-TMT-10 plex Analysis$3550$5325$7100(Up to 10 samples, 14 fractions, including LC-MS/MS and 1 data search)
Quantitative proteomics-TMT-11 plex Analysis$3625$5437.50$7250(Up to 11 samples, 14 fractions, including LC-MS/MS and 1 data search)
Quantitative proteomics-TMT-6 plex Analysis$2960$4440$5920(Up to 6 samples, 14 fractions, including LC-MS/MS and 1 data search)
Quantitative proteomics-TMT-6plex sample labeling kit (not including LC-MS/MS) $800$1200$1600

Reagent

ServicesUFNon-ProfitCommercialDetails
PC Reagents $10$10$10Charge for non routine reagents for specific projects, iTRAQ kits, and etc.

Sample preparation

ServicesUFNon-ProfitCommercialDetails
Abundance Protein removal (concentrates low-abundance proteins), per sample $100$150$200
Buffer exchange $20$25$35
EAE Extraction $45$67.50$90
EZQ protein assay per plate $66$99$132
EZQ protein assay per plate, self-service $45$67.50$90
Phosphopeptide enrichment $90$120$140Services includes various sample preparation steps such as trypsin digestion, desalting, protein assay, and enrichment for protein modifications.
Post-translational modification analysis (inquire)---Services includes various sample preparation steps such as trypsin digestion, desalting, protein assay, and enrichment for protein modifications.
Protein Extraction$350$450$600
Protein Extraction, self-service$100$150-
Protein Precipitation $150$200$250
Solid Phase Extraction, per sample $20$30$40
Spin Column$20$30$40
Trypsin in gel digestion, per sample $20$30$35Sample preparation in protein identification.
Trypsin in solution digestion, per sample $25$35$40Sample preparation in protein identification.
Zip tip, per sample $15$27$35Services includes various sample preparation steps such as trypsin digestion, desalting, protein assay, and enrichment for protein modifications.

Training

ServicesUFNon-ProfitCommercialDetails
MRM Triple Quadruple Mass Spectrometer Training $1500$2250$3000Two day training to learn MRM optimization to quantify peptides and small molecules.
Training 2D-DIGE$600$900$1200
Training 2D-PAGE$300$450$600

Workshop

ServicesUFNon-ProfitCommercialDetails
Proteomics Workshop $300$350$500Proteomics Workshop