Proteomics & Mass Spectrometry
Services & Fees
PROTEOMICS & MASS SPECTROMETRY: Home | Staff & Contact | Services | Instruments | Core Resources
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Offered Services
MRM and "Mass Western" Analysis
MRM quantitative analysis (relative and absolute quantification) of small molecules and peptides/proteins. Price per sample for full service.
MRM Training and Self Service
Two-day training to learn MRM optimization to quantify small molecules and peptides. Price per hour for self service.
Luminex Biomarker Assay
Luminex biomarker immunoassays are antibody-based and there are customized panels for multiplex protein quantitation using the Luminex instrument platform. High-throughput screening up to 500 biomarkers simultaneously.
Luminex Training and Self Service
Protein Identification
Routine protein identification by tandem mass spectrometry from 1D gel bands, 2D gel spots, solution, and immunoprecipitates.
Quantitative Proteomics
Quantitative proteomics analyses. Specific methodology varies with the nature of the sample and the goals of the project.
Post-translational Modification Analysis
Mapping of post-translational modifications or derivatives (e.g. phosphorylation, ubiquitination, acetylation).
Custom Project
Schedule a consultation to discuss custom project needs and prepare statement of work with the Proteomics and Mass Spectrometry team.
1D and 2D Gel Analysis
Analysis includes 1D and 2D gel, 2D DIGE, image analysis, staining, gel excision, and Western Blot.
1D and 2D Gel Instrument Training and Self Service
Sample Fractionation
Separation of proteins and peptides by 3100 OFFGEL Fractionator or HPLC.
Workshop and Training
Workshops
- Quantitative Proteomics
- Data Analysis
Training per request
- Sample preparation
- HPLC Training and Self Service
- Typhoon Scanner Training and Self Service
Service Price List
Last Updated: 08/2019
Direct Infusion
| Services | UF | Non-Profit | Commercial | Details |
|---|---|---|---|---|
| Direct infusion | $15 | $25 | $40 | Service includes directly infusing pure samples to mass spectrometer; no liquid chromatography separation. |
Gel-related services
| Services | UF | Non-Profit | Commercial | Details |
|---|---|---|---|---|
| 1D PAGE mini, 7x8 cm | $75 | $112 | $150 | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot. |
| 1D PAGE mini, 7x8 cm with electroblot | $90 | $130 | $180 | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot |
| 1D PAGE sample prep per protein sample | $5 | $7.5 | $10 | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot. |
| 1D PAGE, 16x16 cm | $125 | $185 | $250 | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot. |
| 1D PAGE, 16x16 cm with Electroblot | $185 | $277 | $370 | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot. |
| 2D PAGE DIGE per gel | $600 | $800 | $1000 | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot. |
| 2D PAGE extra large, 24x24 cm | $225 | $337 | $450 | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot. |
| 2D PAGE extra large, 24x24 cm with blot | $225 | $337 | $450 | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot. |
| 2D PAGE medium, 16x16 cm | $160 | $235 | $300 | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot. |
| 2D PAGE medium, 16x16 cm with blot | $185 | $375 | $500 | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot. |
| 2D PAGE mini, 7x8 cm | $110 | $160 | $200 | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot. |
| 2D PAGE mini, 7x8 cm with blot | $125 | $180 | $225 | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot. |
| 2D PAGE Sample Prep per set of raw samples | $130 | $185 | $240 | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot. |
| 2D PAGE Spot detection per gel | $70 | $105 | $140 | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot. |
| DeCyder image analysis per hour | $60 | $90 | $120 | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot. |
| Excise protein gel plug, manual, per spot | $5 | $7.35 | $10 | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot. |
| Excise protein gel plug, ProPic, price per plate | $40 | $60 | $80 | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot. |
| IEF, Native agarose for large molecules per plate | $150 | $225 | $300 | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot. |
| IEF, Native PAGE | $100 | $150 | $200 | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot. |
| IEF, Native PAGE with electroblot | $120 | $180 | $240 | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot. |
| Preparative IEF using 3100 OFFGEL fractionator | $150 | $200 | $300 | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot. |
| Preparative SDS PAGE using mini prep cell | $200 | $450 | $600 | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot. |
| Protein stain, Colloidal blue stain per 50 ml | $10 | $15 | $20 | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot. |
| Protein stain, Deep purple stain per 50 ml | $25 | $35 | $50 | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot. |
| Protein stain, ProQ Diamond Phosphoprotein stain per 50 ml | $50 | $75 | $100 | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot. |
| Protein stain, ProQ Emerald glycoprotein stain per 50 ml | $60 | $90 | $110 | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot. |
| Protein stain, SilverQuest stain per 50 ml | $15 | $22 | $33 | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot. |
| Protein stain, Sypro Ruby stain per 50 ml | $25 | $35 | $50 | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot. |
| PVDF Membrane | $40 | $56 | $70 | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot. |
| SameSpot image analysis per hour | $80 | $100 | $120 | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot. |
| SameSpot self service image analysis per hour | $60 | $75 | $90 | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot. |
| Self service-1D PAGE mini, 7x8 cm | $15 | $23 | $30 | |
| Self service-2D PAGE DIGE per gel | $300 | $400 | $500 | |
| Self service-2D PAGE extra large, 24x24 cm | $150 | $225 | $300 | |
| Self service-2D PAGE medium, 16x16 cm | $80 | $120 | $150 | |
| Self service-2D PAGE mini, 7x8 cm | $50 | $75 | $90 | |
| Self service-2D PAGE Spot detection per gel (Typhoo scanner) | $25 | $40 | $50 | |
| Self service-Blot PVDF Membrane | $25 | $35 | $45 | |
| Self service-DeCyder image analysis per hour | $40 | $60 | $80 | |
| Self service-Excise protein gel plug, manual, per spot | $1 | $1.50 | $2 | |
| Self service-Excise protein gel plug, ProPic, price per plate | $20 | $30 | $40 | |
| Self service-IEF, Native PAGE | $30 | $45 | $60 | |
| Self service-Preparative IEF using 3100 OFFGEL fractionator | $50 | $90 | $120 | |
| Self service-Preparative SDS PAGE using mini prep cell | $150 | $225 | $300 | |
| Self service-Protein stain, Colloidal blue stain per 50 ml | $10 | $15 | $20 | |
| Self service-Protein stain, Deep purple stain per 50 ml | $25 | $35 | $50 | |
| Self service-Protein stain, ProQ Diamond Phosphoprotein stain per 50 ml | $50 | $75 | $100 | |
| Self service-Protein stain, ProQ Emerald glycoprotein stain per 50 ml | $60 | $90 | $110 | |
| Self service-Protein stain, SilverQuest stain per 50 ml | $15 | $22 | $33 | |
| Self service-Protein stain, Sypro Ruby stain per 50 ml | $25 | $35 | $50 | |
| Special project, per sample | $150 | $225 | $300 | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot. |
| Typhoon self service per scan | $25 | $37.50 | $50 | Service includes 1- and 2-D gel, 2-D DIGE, image analysis, staining, gel excision, and Western blot. |
Instrumental analysis
| Services | UF | Non-Profit | Commercial | Details |
|---|---|---|---|---|
| HPLC (SCX, SE), per sample | $100 | $150 | $200 | Sample analysis on HPLC, LCMS instruments. |
| LC-MS/MS - 1 h gradient for protein ID, per sample | $105 | $168 | $262.50 | Sample analysis on HPLC, LCMS instruments. |
| LC-MS/MS - 1 h gradient for protein ID, per sample if > 50 | $70 | $116.20 | $199.50 | Sample analysis on HPLC, LCMS instruments. |
| LC-MS/MS - 1 h gradient for protein ID, per sample if > 50, Orbitrap-Fusion | $90 | $149.40 | $220.50 | |
| LC-MS/MS - 1 h gradient for protein ID, per sample if >10 | $90 | $135 | $220.50 | Sample analysis on HPLC, LCMS instruments. |
| LC-MS/MS - 1 h gradient for protein ID, per sample if >10, Orbitrap-Fusion | $110 | $165 | $242 | |
| LC-MS/MS - 1 h gradient for protein ID, per sample, Orbitrap-Fusion | $125 | $187.50 | $290 | |
| LC-MS/MS - 1 h gradient for small molecule, per sample, Orbitrap-Fusion with high flow UPLC system | $205 | $307.50 | $410 | |
| LC-MS/MS - 2 hr gradient for protein ID, per sample | $176 | $281.60 | $383.68 | Sample analysis on HPLC, LCMS instruments. |
| LC-MS/MS - 2 hr gradient for protein ID, per sample if > 10 QE Plus | $126 | $189 | $292.32 | Sample analysis on HPLC, LCMS instruments. |
| LC-MS/MS - 2 hr gradient for protein ID, per sample if > 10, Orbitrap-Fusion | $146 | $219 | $292 | |
| LC-MS/MS - 2 hr gradient for protein ID, per sample if > 5 | $151 | $226.50 | $332.20 | Sample analysis on HPLC, LCMS instruments. |
| LC-MS/MS - 2 hr gradient for protein ID, per sample if > 5, Orbitrap-Fusion | $171 | $256.50 | $352.26 | |
| LC-MS/MS - 2 hr gradient for protein ID, per sample if > 50 QE Plus | $111 | $166.50 | $251.97 | Sample analysis on HPLC, LCMS instruments. |
| LC-MS/MS - 2 hr gradient for protein ID, per sample if > 50, Orbitrap-Fusion | $121 | $181.50 | $254.10 | |
| LC-MS/MS - 2 hr gradient for protein ID, per sample, Orbitrap-Fusion | $196 | $313.60 | $401.80 | |
| LC-MS/MS - 3 hr gradient for protein ID, per sample if > 10 Orbtrap Fusion | $210 | $315 | $420 | |
| LC-MS/MS - 3 hr gradient for protein ID, per sample if > 10 QE Plus | $170 | $255 | $340 | |
| LC-MS/MS - 3 hr gradient for protein ID, per sample if > 5 Orbtrap Fusion | $240 | $360 | $480 | |
| LC-MS/MS - 3 hr gradient for protein ID, per sample if > 5 QE Plus | $220 | $330 | $440 | |
| LC-MS/MS - 3 hr gradient for protein ID, per sample if > 50 Orbtrap Fusion | $190 | $315.40 | $380 | |
| LC-MS/MS - 3 hr gradient for protein ID, per sample if > 50 QE Plus | $160 | $240 | $320 | |
| LC-MS/MS - 3 hr gradient for protein ID, per sample Orbtrap Fusion | $270 | $432 | $540 | |
| LC-MS/MS - 3 hr gradient for protein ID, per sample QE Plus | $250 | $400 | $500 | |
| LC-MS/MS - 4 hr gradient for protein ID, per sample if > 10 Orbtrap Fusion | $260 | $390 | $520 | |
| LC-MS/MS - 4 hr gradient for protein ID, per sample if > 5 Orbtrap Fusion | $290 | $435 | $580 | |
| LC-MS/MS - 4 hr gradient for protein ID, per sample if > 50 Orbtrap Fusion | $240 | $360 | $480 | |
| LC-MS/MS - 4 hr gradient for protein ID, per sample Orbtrap Fusion | $320 | $480 | $640 | |
| LC-MS/MS - gel spot or band - 20 min gradient per sample | $82 | $131.20 | $164 | Sample analysis on HPLC, LCMS instruments. |
| LC-MS/MS - gel spot or band - 20 min gradient per sample if > 10 | $57 | $85.50 | $122.55 | Sample analysis on HPLC, LCMS instruments. |
| LC-MS/MS - gel spot or band - 20 min gradient per sample if in house | $47 | $75.20 | $110.92 | Sample analysis on HPLC, LCMS instruments. |
| LC-MS/MS - gel spot or band - 20 min gradient per sample, Orbitrap-Fusion | $102 | $163.20 | $183.60 | |
| LC-MS/MS - gel spot or band - 20 min gradient, per sample if > 10, Orbitrap-Fusion | $77 | $115.50 | $141.68 | |
| LC-MS/MS - gel spot or band - 20 min gradient, per sample if >50 | $37 | $55.50 | $86.21 | Sample analysis on HPLC, LCMS instruments. |
| LC-MS/MS - gel spot or band - 20 min gradient, per sample if >50, Orbitrap-Fusion | $57 | $85.50 | $106.02 | |
| LC-MS/MS-1 h gradient for protein ID, per sample if > 50 LTQ-Orbitrap | $35 | $52.50 | $70 | |
| LC-MS/MS-1 h gradient for protein ID, per sample if >10 LTQ-Orbitrap | $55 | $82.50 | $110 | |
| LC-MS/MS-1 h gradient for protein ID, per sample LTQ-Orbitrap | $65 | $104 | $130 | |
| LC-MS/MS-2 hr gradient for protein ID, per sample if > 10 LTQ-Orbitrap | $96 | $144 | $192 | |
| LC-MS/MS-2 hr gradient for protein ID, per sample if > 5 LTQ-Orbitrap | $116 | $174 | $232 | |
| LC-MS/MS-2 hr gradient for protein ID, per sample if > 50 LTQ-Orbitrap | $71 | $106.50 | $146.26 | |
| LC-MS/MS-2 hr gradient for protein ID, per sample LTQ-Orbitrap | $126 | $201.60 | $252 | |
| LC-MS/MS-gel spot or band-20 min gradient, per sample if >50 LTQ-Orbitrap | $28 | $42 | $58.80 | |
| LC-MS/MS-gel spot or band-20min gradient per sample if > 10 LTQ-Orbitrap | $30 | $45 | $63 | |
| LC-MS/MS-gel spot or band-20min gradient per sample if in house LTQ-Orbitrap | $25 | $41.50 | $53.75 | |
| LC-MS/MS-gel spot or band-20min gradient per sample LTQ-Orbitrap | $32 | $53.12 | $68.80 | |
| MALDI - TOF MS per sample | $35 | $50 | $125 | Sample analysis on HPLC, LCMS instruments. |
Luminex Services
MRM Quantitative Services
| Services | UF | Non-Profit | Commercial | Details |
|---|---|---|---|---|
| 4000 QTRAP MRM Self-Service | $35 | $50.75 | $59.50 | MRM quantitative analysis of peptides and small molecules. Price per hour. |
| MRM-TSQ Altis | $105 | $157.50 | $210 |
Method development
| Services | UF | Non-Profit | Commercial | Details |
|---|---|---|---|---|
| Build selected ion acquisition method | $300 | $450 | $600 | |
| Data Search (add SILAC, PTM) | $60 | $90 | $120 | |
| Method development for special projects, per hour | $60 | $100 | $200 | Method development service for special projects. |
Protein Identification
| Services | UF | Non-Profit | Commercial | Details |
|---|---|---|---|---|
| Additional data search /sample | $20 | $30 | $40 | Additional data search /sample |
Quantitative Analysis
| Services | UF | Non-Profit | Commercial | Details |
|---|---|---|---|---|
| iTRAQ data search | $320 | $480 | $560 | |
| TMT Data Search | $400 | $600 | $800 |
Quantitative Proteomics
| Services | UF | Non-Profit | Commercial | Details |
|---|---|---|---|---|
| iodoTMT-6 plex | $1600 | $2400 | $3200 | |
| MRM-4000 QTRAP, per sample | $45 | $65 | $105 | |
| MRM-Method development (per hour) | $60 | $100 | $200 | |
| PRM-Orbitrap fusion, per sample | $400 | $480 | $800 | |
| PRM-QE plus, per sample | $350 | $420 | $700 | |
| Quantitative Proteomic-Label Free | $200 | $300 | $400 | Set up fee for 1 experiment (2 to 4 samples). |
| Quantitative proteomics-iTRAQ-8 plex Analysis | $3130 | $4695 | $6260 | (Up to 8 samples, 14 fractions, including LC-MS/MS and 1 data search) |
| Quantitative proteomics-TMT-10 plex Analysis | $3550 | $5325 | $7100 | (Up to 10 samples, 14 fractions, including LC-MS/MS and 1 data search) |
| Quantitative proteomics-TMT-11 plex Analysis | $3625 | $5437.50 | $7250 | (Up to 11 samples, 14 fractions, including LC-MS/MS and 1 data search) |
| Quantitative proteomics-TMT-6 plex Analysis | $2960 | $4440 | $5920 | (Up to 6 samples, 14 fractions, including LC-MS/MS and 1 data search) |
| Quantitative proteomics-TMT-6plex sample labeling kit (not including LC-MS/MS) | $800 | $1200 | $1600 |
Reagent
| Services | UF | Non-Profit | Commercial | Details |
|---|---|---|---|---|
| PC Reagents | $10 | $10 | $10 | Charge for non routine reagents for specific projects, iTRAQ kits, and etc. |
Sample preparation
| Services | UF | Non-Profit | Commercial | Details |
|---|---|---|---|---|
| Abundance Protein removal (concentrates low-abundance proteins), per sample | $100 | $150 | $200 | |
| Buffer exchange | $20 | $25 | $35 | |
| EAE Extraction | $45 | $67.50 | $90 | |
| EZQ protein assay per plate | $66 | $99 | $132 | |
| EZQ protein assay per plate, self-service | $45 | $67.50 | $90 | |
| Phosphopeptide enrichment | $90 | $120 | $140 | Services includes various sample preparation steps such as trypsin digestion, desalting, protein assay, and enrichment for protein modifications. |
| Post-translational modification analysis (inquire) | - | - | - | Services includes various sample preparation steps such as trypsin digestion, desalting, protein assay, and enrichment for protein modifications. |
| Protein Extraction | $350 | $450 | $600 | |
| Protein Extraction, self-service | $100 | $150 | - | |
| Protein Precipitation | $150 | $200 | $250 | |
| Solid Phase Extraction, per sample | $20 | $30 | $40 | |
| Spin Column | $20 | $30 | $40 | |
| Trypsin in gel digestion, per sample | $20 | $30 | $35 | Sample preparation in protein identification. |
| Trypsin in solution digestion, per sample | $25 | $35 | $40 | Sample preparation in protein identification. |
| Zip tip, per sample | $15 | $27 | $35 | Services includes various sample preparation steps such as trypsin digestion, desalting, protein assay, and enrichment for protein modifications. |
Training
| Services | UF | Non-Profit | Commercial | Details |
|---|---|---|---|---|
| MRM Triple Quadruple Mass Spectrometer Training | $1500 | $2250 | $3000 | Two day training to learn MRM optimization to quantify peptides and small molecules. |
| Training 2D-DIGE | $600 | $900 | $1200 | |
| Training 2D-PAGE | $300 | $450 | $600 |
Workshop
| Services | UF | Non-Profit | Commercial | Details |
|---|---|---|---|---|
| Proteomics Workshop | $300 | $350 | $500 | Proteomics Workshop |