Proteomics & Mass Spectrometry
Acknowledge Core
Proper Citation:
University of Florida ICBR Proteomics & Mass Spectrometry Core Facility, RRID:SCR_019151
Contact
ICBR-Proteomics@ad.ufl.edu
(352) 273-8060
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MRM quantitative analysis (relative and absolute quantification) of small molecules and peptides/proteins. Price per sample for full service.
Two-day training to learn MRM optimization to quantify small molecules and peptides. Price per hour for self service.
Luminex biomarker immunoassays are antibody-based and there are customized panels for multiplex protein quantitation using the Luminex instrument platform. High-throughput screening up to 500 biomarkers simultaneously.
Routine protein identification by tandem mass spectrometry from 1D gel bands, 2D gel spots, solution, and immunoprecipitates.
Quantitative proteomics analyses. Specific methodology varies with the nature of the sample and the goals of the project.
Mapping of post-translational modifications or derivatives (e.g. phosphorylation, ubiquitination, acetylation).
Schedule a consultation to discuss custom project needs and prepare statement of work with the Proteomics and Mass Spectrometry team.
Analysis includes 1D and 2D gel, 2D DIGE, image analysis, staining, gel excision, and Western Blot.
Separation of proteins and peptides by 3100 OFFGEL Fractionator or HPLC.
Workshops
Training per request
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UF | ICBR Proteomics & Mass Spectrometry | Instrumental Analysis, Luminex Services, MRM Quantitative Services, Method Development, Protein Identification, Quantitative Analysis, Quantitative Proteomics, Reagents, Sample Preparation, Training, Workshops, Consultation, TSQ Altis | TQS Altis, TimSTOF Flex, HPLC, QE Plus | Proteomics & Mass Spectrometry | Visit Proteomics & Mass Spectrometry: Home | Contact | Services | Instruments | Core Resources | Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Instrumental Analysis | Instrumental Analysis | Proteomics & Mass Spectrometry | Contact UF | ICBR Proteomics & Mass Spectrometry for Instrument Analysis Services: ICBR-Proteomics@ad.ufl.edu /// 352.273.8060 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
HPLC (SCX, SE) | Instrumental Analysis | Proteomics & Mass Spectrometry | Sample analysis on HPLC, LCMS instruments. Per sampleUF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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LC-MS/MS - 1 h gradient for protein ID | Instrumental Analysis | Proteomics & Mass Spectrometry | 1 h gradient for protein ID. Sample analysis on HPLC, LCMS instruments. Per sample.UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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LC-MS/MS - 1 h gradient for small molecule, per sample | Instrumental Analysis | Proteomics & Mass Spectrometry | UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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LC-MS/MS - 2 hr gradient for protein ID | Instrumental Analysis | Proteomics & Mass Spectrometry | 2 hr gradient for protein ID. Sample analysis on HPLC, LCMS instruments.UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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LC-MS/MS - 3 hr gradient for protein ID | Instrumental Analysis | Proteomics & Mass Spectrometry | 3 hr gradient for protein ID. Sample analysis on HPLC, LCMS instruments.UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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LC-MS/MS - 4 hr gradient for protein ID | Instrumental Analysis | Proteomics & Mass Spectrometry | 4 hr gradient for protein IDUF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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LC-MS/MS - EvoSep One | Instrumental Analysis | Proteomics & Mass Spectrometry | LC-MS/MS - EvoSep One UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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MALDI - timsTOF fleX MS per sample | Instrumental Analysis | Proteomics & Mass Spectrometry | Sample analysis on HPLC, LCMS instruments.UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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MALDI Image Scan - timsTOF fleX MS per slide (per hour) | Instrumental Analysis | Proteomics & Mass Spectrometry | UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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Luminex Services | Luminex Services | Proteomics & Mass Spectrometry | Contact UF | ICBR Proteomics & Mass Spectrometry for Luminex Services: ICBR-Proteomics@ad.ufl.edu /// 352.273.8060 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Luminex Services | Luminex Services | Proteomics & Mass Spectrometry | No data analysis. Up to 39 sample slots.
UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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MRM Quantitative Services | MRM Quantitative Services | Proteomics & Mass Spectrometry | Contact UF | ICBR Proteomics & Mass Spectrometry for MRM Quantitative Services Services: ICBR-Proteomics@ad.ufl.edu /// 352.273.8060 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
MRM-TSQ Altis | MRM Quantitative Services | Proteomics & Mass Spectrometry | The Thermo Scientific TSQ Altis Triple Quadrupole Mass Spectrometer features segmented quadrupoles with hyperbolic faces, an enhanced dual-mode discrete-dynode electron multiplier detector, and high resolution SRM (0.2 FWHM) for unprecedented sensitivity, selectivity, and speed, as well as unmatched analytical flexibility, reproducibility, and outstanding precision. The TSQ Altis enables every analytical laboratory to successfully address its most demanding applications. It enables every analytical laboratory to successfully address its most demanding applications.
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Method Development | Method Development | Proteomics & Mass Spectrometry | Contact UF | ICBR Proteomics & Mass Spectrometry for Method Development Services: ICBR-Proteomics@ad.ufl.edu /// 352.273.8060 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Build selected ion acquisition method | Method Development | Proteomics & Mass Spectrometry | UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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Custom PTM Database Searching (add SILAC, PTM) | Method Development | Proteomics & Mass Spectrometry | Custom PTM Database Searching (add SILAC, PTM)UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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Method development for special projects, per hour | Method Development | Proteomics & Mass Spectrometry | Method development service for special projects.UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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Protein Identification | Protein Identification | Proteomics & Mass Spectrometry | Contact UF | ICBR Proteomics & Mass Spectrometry for Protein Identification Services: ICBR-Proteomics@ad.ufl.edu /// 352.273.8060 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Additional data search /sample | Protein Identification | Proteomics & Mass Spectrometry | Additional data search /sample.UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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Quantitative Analysis | Quantitative Analysis | Proteomics & Mass Spectrometry | Contact UF | ICBR Proteomics & Mass Spectrometry for Quantitative Analysis Services: ICBR-Proteomics@ad.ufl.edu /// 352.273.8060 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
iTRAQ data search | Quantitative Analysis | Proteomics & Mass Spectrometry | UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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TMT Data Search | Quantitative Analysis | Proteomics & Mass Spectrometry | UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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Quantitative Proteomics | Quantitative Proteomics | Proteomics & Mass Spectrometry | Contact UF | ICBR Proteomics & Mass Spectrometry for Quantitative Proteomics Services: ICBR-Proteomics@ad.ufl.edu /// 352.273.8060 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
iodoTMT-6 plex | Quantitative Proteomics | Proteomics & Mass Spectrometry | iodoTMT-6 plex (Up to 6 samples, labeling and enrichment, not LC-MS/MS and 1 data search)UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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MRM-Method development (per hour) | Quantitative Proteomics | Proteomics & Mass Spectrometry | Per hour.UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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PRM-Orbitrap fusion, per sample | Quantitative Proteomics | Proteomics & Mass Spectrometry | PRM-Orbitrap fusion, per sampleUF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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Quantitative Proteomic-Label Free | Quantitative Proteomics | Proteomics & Mass Spectrometry | Quantitative Proteomic-Label Free, with PRTC internal standards. Set up fee for 1 experiment (2 to 4 samples).UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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Quantitative Proteomics | Quantitative Proteomics | Proteomics & Mass Spectrometry | UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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Sample Preparation | Sample Preparation | Proteomics & Mass Spectrometry | Contact UF | ICBR Proteomics & Mass Spectrometry: ICBR-Proteomics@ad.ufl.edu /// 352.273.8060 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Abundance Protein removal (concentrates low-abundance proteins), per sample | Sample Preparation | Proteomics & Mass Spectrometry | Per sample.UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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Protein Buffer exchange (3 KDa cut-off) | Sample Preparation | Proteomics & Mass Spectrometry | Protein Buffer exchange (3 KDa cut-off)UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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Metabolite EAE Extraction | Sample Preparation | Proteomics & Mass Spectrometry | UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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EZQ protein assay per plate | Sample Preparation | Proteomics & Mass Spectrometry | Per plate.UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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Phosphopeptide enrichment | Sample Preparation | Proteomics & Mass Spectrometry | Services includes various sample preparation steps such as trypsin digestion, desalting, protein assay, and enrichment for protein modifications.UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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Other Post-translational modification enrichment (inquire) | Sample Preparation | Proteomics & Mass Spectrometry | Services includes various sample preparation steps such as trypsin digestion, desalting, protein assay, and enrichment for protein modifications. UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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Protein Extraction | Sample Preparation | Proteomics & Mass Spectrometry | UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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Protein Precipitation | Sample Preparation | Proteomics & Mass Spectrometry | UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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Solid Phase Extraction/Zip tip/Evo tip | Sample Preparation | Proteomics & Mass Spectrometry | Solid Phase Extraction/Zip tip/Evo tip, per sampleUF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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Trypsin/Lys-C in gel digestion, per sample | Sample Preparation | Proteomics & Mass Spectrometry | Trypsin/Lys-C in gel digestion, per sample. Sample preparation in protein identification.UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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Trypsin/Lys-C in solution digestion | Sample Preparation | Proteomics & Mass Spectrometry | Trypsin/Lys-C in solution digestion, per sampleUF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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Self Service Instruments | Self Service Instruments | Proteomics & Mass Spectrometry | Contact UF | ICBR Proteomics & Mass Spectrometry: ICBR-Proteomics@ad.ufl.edu /// 352.273.8060 | Yes | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Agilent 1260 Series Infinity HPLC | Self Service Instruments | Agilent 1260 Series Infinity HLPC | Proteomics & Mass Spectrometry | Agilent 1260 Series Inifinity HPLCUF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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TimsTOF Flex | Self Service Instruments | TimsTOF Flex | Proteomics & Mass Spectrometry | TimsTOF Flex UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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QE Plus | Self Service Instruments | QE Plus | Proteomics & Mass Spectrometry | QE PlusUF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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Training | Consultation, Training | Proteomics & Mass Spectrometry | Contact UF | ICBR Proteomics & Mass Spectrometry: ICBR-Proteomics@ad.ufl.edu /// 352.273.8060 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
MRM Triple Quadruple Mass Spectrometer Training | Training, Consultation | Proteomics & Mass Spectrometry | Two day training to learn MRM optimization to quantify peptides and small molecules.UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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Training 2D-PAGE | Training | Proteomics & Mass Spectrometry | UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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Workshops | Workshops, Consultation, Training | Proteomics & Mass Spectrometry | Contact UF | ICBR Proteomics & Mass Spectrometry: ICBR-Proteomics@ad.ufl.edu /// 352.273.8060 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Proteomics Workshop | Workshops, Consultation, Training | Proteomics & Mass Spectrometry | Inquire about availability and upcoming dates.UF | ICBR Proteomics & Mass Spectrometry /// ICBR-Proteomics@ad.ufl.edu /// 352.273.8060
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Back to top of page | Direct Infusion, Gel-Related Services, Instrumental Analysis, Luminex Services, MRM Quantitative Services, Method Development, Protein Identification, Quantitative Analysis, Quantitative Proteomics, Reagents, Sample Preparation, Training, Workshops, TSQ Altis | TSQ Altis | Proteomics & Mass Spectrometry |
Proper Citation:
University of Florida ICBR Proteomics & Mass Spectrometry Core Facility, RRID:SCR_019151
ICBR-Proteomics@ad.ufl.edu
(352) 273-8060