Proteomics & Mass Spectrometry | Service Fees

ICBR-Proteomics@ad.ufl.edu   ///  (352) 273-8060  ///  RRID:SCR_019151
Gel Electrophoresis, High Throughput Separation, Protein Quantification and More

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SERVICE OVERVIEW

MRM and "Mass Western" Analysis

MRM quantitative analysis (relative and absolute quantification) of small molecules and peptides/proteins. Price per sample for full service.

MRM Training and Self Service

Two-day training to learn MRM optimization to quantify small molecules and peptides. Price per hour for self service.

Luminex Biomarker Assay

Luminex biomarker immunoassays are antibody-based and there are customized panels for multiplex protein quantitation using the Luminex instrument platform. High-throughput screening up to 500 biomarkers simultaneously.

Luminex Training and Self Service

Protein Identification

Routine protein identification by tandem mass spectrometry from 1D gel bands, 2D gel spots, solution, and immunoprecipitates.

Quantitative Proteomics

Quantitative proteomics analyses. Specific methodology varies with the nature of the sample and the goals of the project.

Post-translational Modification Analysis

Mapping of post-translational modifications or derivatives (e.g. phosphorylation, ubiquitination, acetylation).

Custom Project

Schedule a consultation to discuss custom project needs and prepare statement of work with the Proteomics and Mass Spectrometry team.

1D and 2D Gel Analysis

Analysis includes 1D and 2D gel, 2D DIGE, image analysis, staining, gel excision, and Western Blot.

1D and 2D Gel Instrument Training and Self Service

Sample Fractionation

Separation of proteins and peptides by 3100 OFFGEL Fractionator or HPLC.

Workshop and Training

Workshops

  • Quantitative Proteomics
  • Data Analysis

Training per request

  • Sample preparation
  • HPLC Training and Self Service
  • Typhoon Scanner Training and Self Service

SERVICES FEES

UF | ICBR Proteomics & Mass Spectrometry

Instrumental Analysis
HPLC (SCX, SE)
LC-MS/MS - 1 h gradient for protein ID
LC-MS/MS - 1 h gradient for small molecule, per sample
LC-MS/MS - 2 hr gradient for protein ID
LC-MS/MS - 3 hr gradient for protein ID
LC-MS/MS - 4 hr gradient for protein ID
LC-MS/MS - EvoSep One
MALDI - timsTOF fleX MS per sample
MALDI Image Scan - timsTOF fleX MS per slide (per hour)
Luminex Services
Luminex Services
MRM Quantitative Services
MRM-TSQ Altis
Method Development
Build selected ion acquisition method
Custom PTM Database Searching (add SILAC, PTM)
Method development for special projects, per hour
Protein Identification
Additional data search /sample
Quantitative Analysis
iTRAQ data search
TMT Data Search
Quantitative Proteomics
iodoTMT-6 plex
MRM-Method development (per hour)
PRM-Orbitrap fusion, per sample
Quantitative Proteomic-Label Free
Quantitative Proteomics
Sample Preparation
Abundance Protein removal (concentrates low-abundance proteins), per sample
Protein Buffer exchange (3 KDa cut-off)
Metabolite EAE Extraction
EZQ protein assay per plate
Phosphopeptide enrichment
Other Post-translational modification enrichment (inquire)
Protein Extraction
Protein Precipitation
Solid Phase Extraction/Zip tip/Evo tip
Trypsin/Lys-C in gel digestion, per sample
Trypsin/Lys-C in solution digestion
Self Service Instruments
Agilent 1260 Series Infinity HPLC
TimsTOF Flex
QE Plus
Training
MRM Triple Quadruple Mass Spectrometer Training
Training 2D-PAGE
Workshops
Proteomics Workshop
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