Proteomics & Mass Spectrometry

SERVICE OVERVIEW

MRM and "Mass Western" Analysis

MRM quantitative analysis (relative and absolute quantification) of small molecules and peptides/proteins. Price per sample for full service.

MRM Training and Self Service

Two-day training to learn MRM optimization to quantify small molecules and peptides. Price per hour for self service.

Luminex Biomarker Assay

Luminex biomarker immunoassays are antibody-based and there are customized panels for multiplex protein quantitation using the Luminex instrument platform. High-throughput screening up to 500 biomarkers simultaneously.

Luminex Training and Self Service

Protein Identification

Routine protein identification by tandem mass spectrometry from 1D gel bands, 2D gel spots, solution, and immunoprecipitates.

Quantitative Proteomics

Quantitative proteomics analyses. Specific methodology varies with the nature of the sample and the goals of the project.

Post-translational Modification Analysis

Mapping of post-translational modifications or derivatives (e.g. phosphorylation, ubiquitination, acetylation).

Custom Project

Schedule a consultation to discuss custom project needs and prepare statement of work with the Proteomics and Mass Spectrometry team.

1D and 2D Gel Analysis

Analysis includes 1D and 2D gel, 2D DIGE, image analysis, staining, gel excision, and Western Blot.

1D and 2D Gel Instrument Training and Self Service

Sample Fractionation

Separation of proteins and peptides by 3100 OFFGEL Fractionator or HPLC.

Workshop and Training

Workshops

Quantitative Proteomics
Data Analysis

Training per request

Sample preparation
HPLC Training and Self Service
Typhoon Scanner Training and Self Service

SERVICES FEES

UF | ICBR Proteomics & Mass Spectrometry

Instrumental Analysis

HPLC (SCX, SE)

LC-MS/MS - 1 h gradient for protein ID

LC-MS/MS - 1 h gradient for small molecule, per sample

LC-MS/MS - 2 hr gradient for protein ID

LC-MS/MS - 3 hr gradient for protein ID

LC-MS/MS - 4 hr gradient for protein ID

LC-MS/MS - EvoSep One

MALDI - timsTOF fleX MS per sample

MALDI Image Scan - timsTOF fleX MS per slide (per hour)

Luminex Services

MRM Quantitative Services

MRM-TSQ Altis

Method Development

Build selected ion acquisition method

Custom PTM Database Searching (add SILAC, PTM)

Method development for special projects, per hour

Protein Identification

Additional data search /sample

Quantitative Analysis

iTRAQ data search

TMT Data Search

Quantitative Proteomics

iodoTMT-6 plex

MRM-Method development (per hour)

PRM-Orbitrap fusion, per sample

Quantitative Proteomic-Label Free

Quantitative Proteomics

Sample Preparation

Abundance Protein removal (concentrates low-abundance proteins), per sample

Protein Buffer exchange (3 KDa cut-off)

Metabolite EAE Extraction

EZQ protein assay per plate

Phosphopeptide enrichment

Other Post-translational modification enrichment (inquire)

Protein Extraction

Protein Precipitation

Solid Phase Extraction/Zip tip/Evo tip

Trypsin/Lys-C in gel digestion, per sample

Trypsin/Lys-C in solution digestion

Self Service Instruments

Agilent 1260 Series Infinity HPLC

TimsTOF Flex

QE Plus

Training

MRM Triple Quadruple Mass Spectrometer Training

Training 2D-PAGE

Workshops

Proteomics Workshop