BioRad QX200 Digital PCR System

UF | ICBR Gene Expression & Genotyping


Use the QX200 Droplet Digital PCR (ddPCR™) system, the most precise and sensitive PCR solution, for absolute quantification of target DNA molecules.


Features of the QX200 Droplet Digital PCR system include:

  • Flexible digital PCR chemistry – optimized for TaqMan hydrolysis probe and EvaGreen applications
  • Reduced bias from amplification efficiency and PCR inhibitors
  • Flexible assay setup – scalable for high sensitivity or high throughput
  • Convenient assay design – standard curves are not required
  • Simple workflow with 96-sample throughput
  • The QX200 Droplet Digital PCR system comprises:
  • QX200™ droplet generator – partitions each ddPCR reaction mix into 20,000 nanoliter-sized droplets
  • QX200™ droplet reader – streams emulsified droplets in single file past a two-color optical detection system in a serial manner
  • QuantaSoft™ software – performs quantitation of positive/negative droplets and allows visualization in a variety of ways and determine concentrations in copies/µl
  • Associated components and consumables included: DG8™ cartridges, DG8™ gaskets, and cartridge holder


Applications of the QX200 Droplet Digital PCR System

  • Cancer biomarker studies and copy number variation
  • Measure varying degrees of cancer mutations, detect rare DNA target copies, and resolve copy number variation states with superior sensitivity and resolution. PrimePCR™ ddPCR Assays are now available for mutation and copy number detection. These are predesigned, wet-lab validated assays.
  • Pathogen detection
  • Employ the extreme precision of the QX200 System to quantify small fold changes in target DNA or RNA molecules in pathogen detection and monitoring.
  • Next generation sequencing
  • Perform accurate quantification and qualification of NGS libraries without the use of a standard curve.
  • Gene expression analysis
  • Achieve reliable and reproducible measurements of small fold changes for low abundance of mRNA and miRNA.
  • Environmental monitoring
  • Test a wide variety of environmental samples like soil and water using the QX200 System.
  • Food testing
  • Perform routine evaluation of genetically modified organisms (GMO) using validated ddPCR methods.


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