Lunch and Learn: Bruker Spatial Biology
Integrated Single-Cell Functional Discovery with Beacon® — From Antibody Discovery to TCR Functional Screening
Join us for a focused seminar to learn how researchers can perform single-cell, real-time analysis of B and T cells to identify high-affinity antibodies, characterize TCR specificity, and accelerate candidate selection with direct functional readouts. The session will cover workflows for Antibody Discovery and T Cell Profiling applications; including cytotoxicity, cell surface and cytokine profiling, cell–cell interaction assays, and clone recovery for downstream sequencing and engineering.
The Beacon® platform enables researchers to sort and analyze tens of thousands of live cells with a variety of functional assays over time. Cell sorting is accomplished with light, preserving cell health and allowing for the creation of complex co-cultures. Analysis is done in single-cell microfluidic chambers, unlocking the ability to measure complex biology at single cell resolution. Assays are performed on live cells, and data is captured over-time to provide dynamic and longitudinal insights. Finally, single cells are collected post-assay for scale-up or downstream molecular analysis. These integrated capabilities have accelerated development of therapeutics, diagnostics, and reagents; and has identified novel biomarkers for complex biologics. Attendees will leave the seminar understanding:
- The Beacon Platform’s Technical Capabilities and Applications
- Antibody Discovery & BCR/TCR Repertoire Analysis – Sequencing B cells to develop antibodies against known and novel /unknown antigens in one day
- Cell-Cell Interaction Analysis – Characterizing T cell cytotoxicity, cytokine secretion, cell surface protein expression, and cell motility in live cell co-cultures to understand functionality of CARs/TCRs/BiTEs
- Functional Biology + Spatial Biology – A case study interrogating functional TCRs in a patient tumor and spatially mapping T cell receptors to understand cancer pathology
Speakers:
Joseph Valdez, Ph.D. | Technical Sales Manager
Dr. Valdez accelerates researchers’ goals by translating their operations onto the Beacon. Dr. Valdez trained in molecular and cellular analysis in the field of oncology and holds deep experience in cutting-edge analytical methods
Amanda M. Dossat, Ph.D. | Field Applications Scientist
Dr. Dossat brings a strong background in molecular biology and high-content confocal imaging. Dr. Dossat has applied these tools across neuroscience, immunology, oncology, and multi-omics research domains.