GENERAL GUIDES & SOFTWARE

iLab Account Registration and Manual

Biowaste Handing, Control & Responsibilities

Management of Animal Diet Autofluorescence

Recognition of Components of the Vaporizer

Facsdivav6_lsr_quickrefguide

FCS_Express_information_email

Fluorofinder (light)

CYTOMETRY MATERIALS

Flow Cytometry Basics Guide

Minimum Information About a Flow Cytometry Experiment (MIFlowCyt)

University of Florida Sorting Contract

Titrating Antibodies on Human PBMC

Sorting Buffer Recipe

Distributions of Autofluorescence After Compensation: Be Panglossian, Fret Not

Spectral Compensation for Flow Cytometry: Visualization Artifacts, Limitations, and Caveats

BEST PRACTICES

MiSet RFC Standards: Defining a Universal Minimum Set of Standards Required for Reproducibility and Rigor in Research Flow Cytometry Experiments

Rigor and Reproducibility in Flow (Tim Bushnell)

Reproducibility in Flow Cytometry through Standardization

NIH Enhancing Reproducibility Guidelines

Scientific Rigor and Reproducibility (Kemp, 2016)

Rigor and Reproducibility of Cytometry Practices for Immuno-Oncology (Laskowski 2020)

Flow Repository (Spidlen 2012)

Click Here for Cytometry Instrument-Specific Resources

GENE EXPRESSION GUIDLEINES & FAQS

10X-Genomics-Sample-requirements-All-libraries_080219

16S-metagenomics_FAQs_080219

Bioanalyzer-TapeStation-FAQs_080219

Cell-Line-FAQs_080219

ddPCR-FAQs_080219

Fragment-FAQs_080219

RNA-Seq_FAQs_080219

NEXTGEN DOCUMENTS

10X-Genomics-scRNAseq-lib-seq-recommendations

ELF-for-PacBio-library-fragment-size-selection

Illumina-16S-metagenomics_NGS-lab-recomm

NGS_Best-practices_Notes

NGS-QC-Metrics

PacBio_gel-purified-fragments-recommend

PacBio-IsoSeq-workflow-on-the-SEQUEL_111518-

WGA-options

PROTEOMICS PROTOCOLS 

Isobaric labeling Quantification (iTRAQ/TMT/iodoTMT): Isobaric-Labeling-Quantification

SILAC: SILAC

Protein Extraction: Protein-isolation-using-Phenol

PTM Sample Preparation and Analysis: PTM-Sample-Prep-and-Analysis

MRM/SRM and PRM: MRM-SRM-PRM

Protein N-terminal Labeling and Analysis: N-Terminal-Labeling-and-Analysis

Label-Free Shotgun Proteomics: Label-Free-Shotgun

2D-DIGE: 2D-DIGE

PROTEOMICS DELIVERABLES AND DATA VISUALIZATION 

Deliverables

• -iTRAQ: Raw files and ProteinPilot 5.0 or Scaffold with Mascot 2.6 output (please choose one)
• -TMT: Raw files and Proteome Discoverer 2.2 output
• -Label-Free Proteomics: Raw files and Scaffold Q+S with Mascot 2.6 or Proteome Discoverer 2.2 output
• -identification: Raw files and Scaffold Q+S with Mascot 2.6 (*.dat)

Scaffold Viewer

www.proteomesoftware.com/products/qplus-s/

ProteinPilot Viewer

sciex.com/products/software/proteinpilot-software

Proteome Discoverer Viewer

thermo.flexnetoperations.com/

Mascot Public Search

www.matrixscience.com/cgi/search_form.pl?FORMVER=2&SEARCH=MIS

Gatorlink Request Form

2023 48×36 ICBR Poster

UF ICBR Brochure