The NanoFCM is a nano-flow cytometry instrument that allows the measurement of liposomes, nanoparticles, biological particles such as extracellular vesicles (otherwise known as EVs or exosomes) and viruses when fluorescently labelled or not. Equipped with two lasers (Blue and Red), two fluorescence detectors and one side scatter detector. It can measure particle size, size distribution concentration and the presence of markers stemming from fluorescence labelling in only one-minute run time. This device is compatible with a wide range of labelling strategies, such as GPF, immuno-labelling with fluorescently tagged antibodies, nucleic acid dyes and membrane dyes. The NanoFCM dynamic measurement range for extracellular vesicles 40nm to 1μm, which covers most of the exosome and microvesicles fractions

Summary of the NanoFCM features:

• Scatter sensitivity: <40 nm silica nanoparticles
• Particle concentration measurement (particles/mL)
• Single photon counting avalanche photodiode detectors
• Particle integration time control for high-sensitivity detection
• Real-time, individual particle trace event: removes risk of swarm-detection
• 30μL minimum sampling volume
• Simultaneous measurement of size and concentration for fluorescently labelled samples
• Calibration standards for size, concentration and fluorescence
• One-minute measurement time
• Scatter-triggering of signal detection: no risk of false-positive events compared to fluorescence triggering detection