Olympus IX81-DSU Spinning Disk Confocal Microscope

Two filter based spinning disk confocal microscopes with Z-drive for basic confocal Z-stack imaging. We have 2 of these systems, one in our MBI building location equipped with a motorized XY stage, and the other, in our CGRC location, equipped with a manually controlled XY stage. Inverted configuration.

Objectives: 10x dry, 20x dry, 20x dry LWD, 40x dry and 60x water (60x BROKEN)

Fluorescent filter sets: DAPI, GFP/FITC, TRITC, Texas Red, and CY5

LED fluorescence excitation light source: CoolLED pE-300 (white) with 3 LED channels.

Camera: Hamamatsu Flash 4.0 LT (16 bit monochrome) sCMOS

Software: Olympus cellSens

Two locations: MBI Imaging Suite (MBI room LG-164), and CGRC Cytometry core (CGRC room 292).

Designed by Olympus, the DSU disk contains a pattern of slits that creates a virtual pinhole as the disk spins at 3,000 rpm. Designed to optimize the tradeoff between confocality and light throughput, the DSU uses an LED illumination source for fluorescence excitation.