New COVID test can quickly detect variants

New COVID test can quickly detect variants

from UF News

University of Florida researchers have invented a test that can determine within 10-15 minutes whether patients test positive for COVID and, if so, which of the five known variants of concern they have.

The research, published Saturday in The Lancet’s eBioMedicine, involves the use of a simple heating device and a cellphone. The team also used a new form of CRISPR — a means of finding and targeting a specific section of genetic material inside a cell — to quickly and effectively diagnose COVID and learn which variant of concern is in a patient sample: alpha, beta, gamma, delta, or omicron.

The finding could provide policymakers with vital information about when to require masking and prepare hospitals for a wave of infections. It could also allow patients and family members to prevent or treat a particular variant more effectively.

“For the medical and research community, knowing which variants are emerging is extremely important,” said corresponding author Piyush Jain, an assistant professor and a Shah Rising Star Professor in the Department of Chemical Engineering. “If an especially serious variant like delta or a genetically similar variant, such as deltacron, arises in the future, work like this discovery will help us track it earlier.”

The team, including professors Marco Salemi, Rhoel Dinglasan, John Lednicky and their lab members from the Emerging Pathogens Institute, made the findings in collaboration with two bioscience engineering companies registered in the Czech Republic. The companies helped the researchers engineer a prototype that could function at home, in a clinic, or in the field.

Current processes for determining COVID variants take several days to perform, which is why patients typically don’t learn which variant they have.

Advancing on recently published work, including by Long Nguyen, a PhD candidate and the first author of the study, the UF team is the first to investigate the new heat-loving CRISPR technology, which manages to combine speed, accuracy, and variant identification by amplifying and detecting coronavirus genetic material in one step.


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Dr. Piyush Jain is a user in ICBR’s Cytometry and Monoclonal Antibody cores.
Dr. Rhoel Dinglasan is a user in ICBR’s Cytometry, Electron Microscopy, Monoclonal Antibody, NextGen DNA Sequencing, and Gene Expression & Genotyping cores.
Dr. Marco Salemi is a user in ICBR’s Bioinformatics, Cytometry, NextGen DNA Sequencing and Gene Expression & Genotyping cores.
Dr. John Lednicky is a current user in UF | ICBR’s  Electron Microscopy and Gene Expression & Genotyping cores.