Access focused applications such as targeted resequencing, metagenomics, small whole-genome sequencing, targeted gene expression profiling, and more. MiSeq i100 reagents enable up to 30 Gb of output with 100 million sequencing reads and 2 × 300 bp read lengths.
The MiSeq i100 Series are powered by XLEAP-SBS chemistry, the fastest, most accurate, Illumina sequencing by synthesis (SBS) chemistry to date. Improved robustness and stability of XLEAP-SBS reagents allow for shipping and storage at room temperature, expediting run setup without waiting for reagents to thaw. MiSeq i100 Series generates highly accurate data with a minimum specification of > 90% of bases above Q30. Enhanced usability features, including load-and-go consumables, onboard denaturation, onboard cluster generation, and no post-run washing, streamline the sequencing workflow.
| Flow Cell | Cycles | Read Length | Reads Passing Filter | Output | Run Time |
| 5M | 300 | 2 x 150 bp | 5 Million | 1.5 Gb | 7 hr |
| 600 | 2 × 300 bp | 3 Gb | 15 hr | ||
| 25M | 100 | 2 x 50 bp | 25 Million | 2.5 Gb | 4 hr |
| 300 | 2 x 150 bp | 7.5 Gb | 7 hr | ||
| 600 | 2 × 300 bp | 15 Gb | 15 hr | ||
| 50M | 100 | 2 x 50 bp | 50 Million | 5 Gb | 4.5 hr |
| 300 | 2 x 150 bp | 15 Gb | 7.5 hr | ||
| 600 | 2 × 300 bp | 30 Gb | 15.5 hr | ||
| 100M | 100 | 2 x 50 bp | 100 Million | 10 | 5 hr |
| 300 | 2 x 150 bp | 30 Gb | 8 hr |
Link: https://www.illumina.com/systems/sequencing-platforms/miseq-i100.html