ICBR Cytometry Acquires BD FACS Symphony S6
The recent acquisition of the BD FACS Symphony S6 cell sorter in the ICBR Cytometry Core will expand the capabilities of the UF research community to isolate rare and unique populations of cells from a complex, mixed populations. Cell sorting on the S6 instrument facilitates the isolation of enriched cell pools and individual cells for downstream genomic analysis using DNA sequencing, as well as RNA-seq analysis to identify, quantify and profile mRNAs at unprecedented levels of detail. Compared to the existing BD FACS Aria sorters, the S6 increases the number of parameters (light scatter, fluorochromes, dyes) from 17 to 28, and the number of possible gating tiers from 8 to 16, allowing the construction of much more complex sorting panels.
Furthermore, it increases the number of sort streams from 4 to 6 distinct populations and allows sorting into both 96- and 384-well plates. The configuration of the Symphony S6 matches with the configuration of the Symphony A3 analyzer, so experiments optimized on the A3 can be seamlessly transferred onto the S6. And finally, the S6 also takes advantage of the latest improvements in imaging and sorting technology to increase sensitivity, precision and reliability across the board, so studies initiated on the Aria sorters can be performed with more precision and sensitivity on this newer advanced instrument.