Cytometry |  Services

ICBR-Cytometry@ad.ufl.edu   ///  (352) 273-6032   ///    RRID:SCR_019119
Cytometers, Histology, Confocal Microscopes, In-vivo Imaging

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SERVICES OVERVIEW

NOTE: Cytometry services fees are currently being updated as of 9/15-9/20. Please refer to iLab for most up to date pricing.

Cell Sorting - Staff-Assisted and Full-Service

Flow cytometry sorting analysis is used to identify sub-populations for enrichment. We have large instruments that can detect up to 17 Fluorescent parameters. We offer both staff assisted sorting as well as training for self-service usage on our smaller Sony SH00 sorter. Cells can be sorted into 4 populations of interest can be sorted into Tubes (5ml FACS, 12ml Conicals) or 96 well plates. We can sort single cells into each well of the 96 well plate and index map them back to the original population of interest within the software. Customers will receive data and purified cells. ICBR staff is available to advise on specific sample requirements.

Confocal Imaging - Full-Service

An ICBR staff member will work interactively with customers to produce confocal images from live or fixed preparations. The customer is responsible for preparing stained slides or cell cultures.

Drop Off Flow Cytometry Sample Analysis

An ICBR staff member will analyze samples prepared by the customer and produce a report containing selected graphs and statistics. Prepared samples must contain dissociated cells (or subcellular particles) in suspension, usually stained with one or more fluorescent dyes.

Equipment Training

Equipment Training is available to all users, and must be requested in ilab. All equipment training will be scheduled by CY staff at their earliest availability and is intended for those who already have some knowledge of flow cytometry. Training for novice users is carried out interactively using samples prepared by the customer.

Project Consultation

Schedule a consultation to discuss custom project needs and prepare a project outline with an ICBR staff member.

This is required for all new sorting projects or any cellular drop off projects where Flow Core Staff will be running samples for customers.

Request Flowjo Login Account

Users may place a request in iLab for FlowJo annual license key.

Access is provided to UF Staff/Students if you are a registered self-service user of the Cytometry Core instruments. Access is time-limited, based on your continued use of the lab.

Training Courses

  • Flow Cytometry Course I/II

SERVICE FEES