NextGen DNA Sequencing

ICBR-NextGenSeq@ad.ufl.edu   ///  (352) 273-8050   ///    RRID:SCR_019152
High-throughput Sequencing Services, Library Construction, Fragmentation and More

Overview

CORE OVERVIEW

Welcome to the UF ICBR NextGen DNA Sequencing core facility. ICBR NextGen DNA Sequencing provides quality, massively parallel, high-throughput sequencing data. We currently support the following platforms: PacBio (Revio), Illumina (NovaSeq X Plus, MiSeq i100), Oxford NanoPore (PromethION-24, MinION Mk1c). These instruments allow for a broad range of sequencing applications for biological research. Customers are encouraged to consult with core staff for the purpose of tailoring data acquisition to specific research questions.

SERVICES OVERVIEW

Don’t see the service you are looking for? Visit UF | ICBR Gene Expression & Genotyping for more options.

Sequencing Services

Library Construction Services

Sample/Library QC

General Services

Sequencing

    • Illumina NovaSeq X Plus (high-throughput and high-plexicity projects , any run configuration, partial lane flexibility)
    • Illumina MiSeq i100 (metagenomics, small whole-genome, amplicon sequencing)
    • PacBio Revio (Hi-Fi long reads for de novo genome assembly and isoform analysis, etc.)
    • Oxford NanoPore PromethION-24 (Ultra-long reads for de novo genome assembly of high-throughput projects)
    • Oxford NanoPore MinION (Ultra-long reads for de novo genome assembly of small genomes or pilot runs)

Library Construction Services for Major Applications (in conjunction with GE/GT core):

    • Illumina (shotgun-genome, WGBS, RRBS, 16S metagenomics, COVID-seq, RNA-seq, Exome-seq)
    • PacBio (large-insert genome, Iso-Seq, Amplicon-seq, full-length 16S)
    • Oxford NanoPore (large-insert genome, full-length cDNA, Direct RNA-seq, full-length 16S, COVID-seq)
    • 10X Genomics (Single-cell: RNA-Seq, ATACseq, VDJ)

 

Sample/Library QC

    • Qubit
    • Covaris S220, E220
    • qPCR
    • TapeStation
    • SAGE ELF
  • Project Consultation
  • TapeStation
  • Qubit Assays
  • Assisted use of Covaris for sample fragmentation
  • Fragment Selection (ELF-based)
  • Sample/library clean-up
  • Load only sequencing instrument runs
  • Library pooling and pool quantitation

Please note that our library construction and sequencing services do not include data processing. Visit ICBR Bioinformatics to request Data Analysis services.

CORE NEWS

UF Investigators Identify Mechanism of Drug Resistance in Non-Small Cell Lung Cancer
Stars Align for Science: University of Florida and NSF NRAO Forge Path for Discovery
New Instruments in the ICBR NGS Shared Resource Lowers DNA Sequencing Costs for UF Investigators
UF plant biologist Anna-Lisa Paul receives SURA’s Distinguished Scientist Award
ICBR Closed Friday (7/18) for Team Building
ICBR Closed Friday for the 4th of July
Environmental DNA – It’s All Up in the Air
ICBR Closed Thursday for Juneteenth
UF plant biologist Anna-Lisa Paul receives SURA’s Distinguished Scientist Award
ICBR Closed Friday (7/18) for Team Building
ICBR Closed Friday for the 4th of July
Environmental DNA – It’s All Up in the Air
ICBR Closed Thursday for Juneteenth
UF Investigators Identify Mechanism of Drug Resistance in Non-Small Cell Lung Cancer
Stars Align for Science: University of Florida and NSF NRAO Forge Path for Discovery
New Instruments in the ICBR NGS Shared Resource Lowers DNA Sequencing Costs for UF Investigators

CORE INSTRUMENTS

NextGen DNA Sequencing

CORE PERSONNEL