NextGen DNA Sequencing

Overview

CORE OVERVIEW

Welcome to the UF ICBR NextGen DNA Sequencing core facility. ICBR NextGen DNA Sequencing provides quality, massively parallel, high-throughput sequencing data. We currently support the following platforms: PacBio (SEQUEL IIe), Illumina (NovaSeq X Plus, MiSeq), Oxford NanoPore (PromethION-24, MinION Mk1c). These instruments allow for a broad range of sequencing applications for biological research. Customers are encouraged to consult with core staff for the purpose of tailoring data acquisition to specific research questions.

Our sample prep and library construction services are offered through both the NGS and the Gene Expression/Genotyping Cores. Please contact us if the service of your interest is not listed. We strive to support most common applications.

SERVICES OVERVIEW

Don’t see the service you are looking for? Visit UF | ICBR Gene Expression & Genotyping for more options.

Sequencing Services

Library Construction Services

Sample/Library QC

General Services

Sequencing

- Illumina NovaSeq X Plus (high-throughput and high-plexicity projects , any run configuration, partial lane flexibility)
- Illumina MiSeq (Standard v3 and v2 runs, Micro and Nano flow cell runs for pilot runs)
- Illumina iSeq (for shallow, pilot runs)
- PacBio SEQUEL IIe (Hi-Fi long reads for de novo genome assembly and isoform analysis, etc.)
- Oxford NanoPore PromethION-24 (Ultra-long reads for de novo genome assembly of high-throughput projects)
- Oxford NanoPore MinION (Ultra-long reads for de novo genome assembly of small genomes or pilot runs)

Library Construction Services for Major Applications (in conjunction with GE/GT core):

- Illumina (shotgun-genome, WGBS, RRBS, 16S metagenomics, COVID-seq, RNA-seq, Exome-seq)
- PacBio (large-insert genome, IsoSeq, Amplicon-seq, full-length 16S)
- Oxford NanoPore (large-insert genome, full-length cDNA, Direct RNA-seq, full-length 16S, COVID-seq)
- 10X Genomics (Single-cell: RNA-Seq, ATACseq, VDJ)

Sample/Library QC

- QUBIT
- Covaris S220, E220
- qPCR
- TapeStation
- SAGE ELF

Project Consultation
TapeStation
Qubit Assays
Assisted use of Covaris for sample fragmentation
Fragment Selection (ELF-based)
Sample/library clean-up
Load only sequencing instrument runs
Library pooling and pool quantitation

Please note that our library construction and sequencing services do not include data processing. Visit ICBR Bioinformatics to request Data Analysis services.

CORE NEWS

ICBR Closed for the Holidays!

Wishing you and yours a Happy Holiday and a joyful New Year. Best wishes from your friends at UF | ICBR! ICBR will be closed beginning at noon on Tuesday, December 24th through Wednesday, January 1st. We here at ICBR are thankful for you, and hope you enjoy the holiday! And a special thank you the near thousand users that came through our doors this year, including 300+ PIs from over a hundred UF departments! We look forward to working with you 2025! Normal business hours will resu...

ICBR Closed for the Thanksgiving Holiday!

Wishing you and yours a joyous Thanksgiving. Best wishes from your friends at UF | ICBR! ICBR will be closing early Wednesday afternoon at 3pm, and will be closed all day Thursday and Friday (November 28th and 29th); returning on Monday (December 2nd). We here at ICBR are thankful for you, and hope you enjoy the holiday! And a special thank you the near thousand users that came through our doors this year, including 300+ PIs from over a hundred UF departments! We look forward to working with you...

ICBR Billing Schedule Update: Key Dates for Calendar Year End 2024

Please note: Due to the Calendar Year End, the last invoices to process for 2024 will be created on Monday, December 9th. ICBR’s upcoming billing schedule will be altered as follows: 12/9/2024 – Last invoices generated in 2024 12/16/2024 at noon – Deadline to submit disputes for 2024 payment. If you have expiring funds, please work with your core laboratory contact to make sure your project is completed and billed before the 12/9/2024 invoices are posted. ...

ICBR Closed Monday for Veterans Day

ICBR Closed Friday for Veterans Day ICBR will be closed this Monday (11/11) in observation of Veterans Day We will not be accepting packages or samples on Monday. Our normal business hours will resume on Tuesday (11/12). Enjoy the the long weekend! Please refer to our staff directory for additional details. ...

ICBR and campus closed Wednesday and Thursday due to Hurricane Milton

Due to Hurricane Milton, UF ICBR will be closed Wednesday, October 9th and Thursday, October 10th, and will reopen and resume normal operations at 8am on Friday. We will not be accepting packages or samples on Wednesday or Thursday. Our normal business hours will resume on Friday. Please stay safe! Additional UF Info: Due to Hurricane Milton, the University of Florida will close its offices and cancel classes, including online classes, beginning at 12:01 a.m. Wednesday, Oct. 9 and continuing th...

ICBR Hosts Successful Inaugural Research Day

ICBR Hosts Successful Inaugural Research Day The University of Florida Interdisciplinary Center for Biotechnology Research (ICBR) recently held its inaugural ICBR Research Day. The 2024 event was a great success, attended by over 80 scientists representing the various colleges and departments across campus. The event began with two plenary talks followed by 4 separate sessions where 12 speakers presented their research and groundbreaking discoveries made possible by the support and expertise of ...

UF ICBR Empowering Research Excellence and Innovation at the University of Florida

UF ICBR Empowering Research Excellence and Innovation at the University of Florida The University of Florida Interdisciplinary Center for Biotechnology Research (UF ICBR) proudly continues to enable scientific discovery and innovation across the University of Florida research community. We provide access to world-class biotechnology expertise and instrumentation, essential for advancing research. UF Research announced impressive achievements in their 2023 Annual Report, including $1.096 billion ...

Turning Up the Heat on Tumors – ICBR Supports Landmark Study in Novel Cancer Vaccine Strategy

Turning Up the Heat on Tumors - ICBR Supports Landmark Study in Novel Cancer Vaccine Strategy

Turning Up the Heat on Tumors – ICBR Supports Landmark Study in Novel Cancer Vaccine Strategy The ICBR Cytometry and Electron Microscopy Shared Resources played an integral part in supporting a landmark study from UF cancer researchers published in the May 1 issue of the prestigious journal Cell. The study, led by physician scientist Dr. Elias Sayour, a UF Health pediatric oncologist and co-leader of the UF Health Cancer Center’s Immuno-Oncology and Microbiome research program, represents...

CORE INSTRUMENTS